WO2000075286A3 - 3-dimensional in vitro models of mammaliam tissues - Google Patents

3-dimensional in vitro models of mammaliam tissues Download PDF

Info

Publication number
WO2000075286A3
WO2000075286A3 PCT/CA2000/000650 CA0000650W WO0075286A3 WO 2000075286 A3 WO2000075286 A3 WO 2000075286A3 CA 0000650 W CA0000650 W CA 0000650W WO 0075286 A3 WO0075286 A3 WO 0075286A3
Authority
WO
WIPO (PCT)
Prior art keywords
models
dimensional
tissues
mammaliam
novel
Prior art date
Application number
PCT/CA2000/000650
Other languages
French (fr)
Other versions
WO2000075286A2 (en
Inventor
Herve Jouishomme
Jenny Phipps
Michel Phipps
Suzanne Lacelle
Original Assignee
Ca Nat Research Council
Herve Jouishomme
Jenny Phipps
Michel Phipps
Suzanne Lacelle
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ca Nat Research Council, Herve Jouishomme, Jenny Phipps, Michel Phipps, Suzanne Lacelle filed Critical Ca Nat Research Council
Priority to EP00934826A priority Critical patent/EP1185619A2/en
Priority to AU50560/00A priority patent/AU784149B2/en
Priority to CA002375788A priority patent/CA2375788A1/en
Priority to IL14689200A priority patent/IL146892A0/en
Publication of WO2000075286A2 publication Critical patent/WO2000075286A2/en
Publication of WO2000075286A3 publication Critical patent/WO2000075286A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/25—Urinary tract cells, renal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/30—Coculture with; Conditioned medium produced by tumour cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/04—Screening or testing on artificial tissues

Abstract

Novel 3-dimensional in vitro models of mammalian tissues are provided. In a preferred embodiment, the models simulate behavior of human cancerous tissues at various stages of a solid tumour progression or the cell senescence, apoptosis or tissue regeneration in normal or cancerous human cells. The models are particularly useful for screening and testing of anticancerous drugs and assessing their toxicity for both the tumour and the surrounding tissue. Novel cellular automaton models for analysis and simulation of 3-dimensional cell organoids have also been developed. These models are useful for studying intercellular communication and predicting effects of gap junction channels modulating drugs.
PCT/CA2000/000650 1999-06-03 2000-06-02 3-dimensional in vitro models of mammaliam tissues WO2000075286A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP00934826A EP1185619A2 (en) 1999-06-03 2000-06-02 3-dimensional in vitro models of mammaliam tissues
AU50560/00A AU784149B2 (en) 1999-06-03 2000-06-02 3-dimensional in vitro models of mammaliam tissues
CA002375788A CA2375788A1 (en) 1999-06-03 2000-06-02 3 dimensional cell system (organoids) and associated protocols
IL14689200A IL146892A0 (en) 1999-06-03 2000-06-02 3-dimensional in vitro models of mammalian tissue

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13759699P 1999-06-03 1999-06-03
US60/137,596 1999-06-03

Publications (2)

Publication Number Publication Date
WO2000075286A2 WO2000075286A2 (en) 2000-12-14
WO2000075286A3 true WO2000075286A3 (en) 2001-05-03

Family

ID=22478179

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2000/000650 WO2000075286A2 (en) 1999-06-03 2000-06-02 3-dimensional in vitro models of mammaliam tissues

Country Status (5)

Country Link
EP (1) EP1185619A2 (en)
AU (1) AU784149B2 (en)
CA (1) CA2375788A1 (en)
IL (1) IL146892A0 (en)
WO (1) WO2000075286A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003025157A1 (en) * 2001-09-14 2003-03-27 The Genetics Company A coculture system to identify proteins triggering redifferentiation of tumor cells
BR0307279A (en) 2002-01-29 2004-12-28 Wyeth Corp Methods for closing and opening a semichannel and a cell, organic tissue or organ, to prevent or treat the stress of organic tissue or organ in a mammal, to increase intracellular communication of the gap junction in a cell, organic tissue or organ. for the treatment of burns, thrombosis, respiratory and metabolic acidosis, and focal arrhythmia, for the treatment and prevention of cell and organ tissue damage resulting from elevated blood glucose levels, for the treatment of chronic atrial fibrillation and epilepsy, to create compound candidates that modulate semi-channel function, to cytoprotect organic tissue or an organ of a mammal in need of such treatment, and to prevent or treat reperfusion damage in a mammal
US10006904B2 (en) * 2011-12-19 2018-06-26 Umc Utrecht Holding B.V. Rapid quantitative assay to measure CFTR function in a primary intestinal culture model
JP7316745B2 (en) * 2016-05-20 2023-07-28 株式会社リコー three-dimensional organization
WO2017199820A1 (en) 2016-05-20 2017-11-23 Ricoh Company, Ltd. Three-dimensional tissue
WO2018071797A1 (en) * 2016-10-14 2018-04-19 Wake Forest University Health Sciences Compositions, cell constructs, and methods of making and using the same
WO2019048689A1 (en) 2017-09-11 2019-03-14 Imba - Institut Für Molekulare Biotechnologie Gmbh Tumor organoid model
CN116539862B (en) * 2022-07-20 2024-03-12 成都诺医德医学检验实验室有限公司 Method for testing tumor metastasis capability based on organoid symbiotic long platform
CN115927164B (en) * 2022-10-11 2023-09-26 成都诺医德医学检验实验室有限公司 Culture method and application of vascularized tumor organoids

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5314805A (en) * 1991-10-28 1994-05-24 Molecular Probes, Inc. Dual-fluorescence cell viability assay using ethidium homodimer and calcein AM
US5518915A (en) * 1986-04-18 1996-05-21 Advanced Tissue Sciences, Inc. Three-Dimensional mucosal cell and tissue culture system

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5518915A (en) * 1986-04-18 1996-05-21 Advanced Tissue Sciences, Inc. Three-Dimensional mucosal cell and tissue culture system
US5314805A (en) * 1991-10-28 1994-05-24 Molecular Probes, Inc. Dual-fluorescence cell viability assay using ethidium homodimer and calcein AM

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DE RIDDER L: "Autologous confrontation of brain tumor derived spheroids with human dermal spheroids.", ANTICANCER RESEARCH, vol. 17, no. 6B, November 1997 (1997-11-01), pages 4119 - 4120, XP000971885, ISSN: 0250-7005 *
FRESHNEY R I: "Culture of animal cells", 1987, ALAN R. LISS, NEW YORK, US, XP002157618 *
NYGAARD S J T ET AL: "Glioma cell invasion visualized by scanning confocal laser microscopy in an in vitro co-culture system.", INVASION & METASTASIS, vol. 15, no. 5-6, 1995, pages 179 - 188, XP000971895, ISSN: 0251-1789 *
NYGAARD SVEIN J T ET AL: "Dynamic determination of human glioma invasion in vitro.", JOURNAL OF NEUROSURGERY, vol. 89, no. 3, 1998, pages 441 - 447, XP000971892, ISSN: 0022-3085 *

Also Published As

Publication number Publication date
IL146892A0 (en) 2002-08-14
EP1185619A2 (en) 2002-03-13
AU5056000A (en) 2000-12-28
CA2375788A1 (en) 2000-12-14
WO2000075286A2 (en) 2000-12-14
AU784149B2 (en) 2006-02-09

Similar Documents

Publication Publication Date Title
WO2003083044A3 (en) Tissue analogs for in vitro testing and method of use therefor
WO2000075286A3 (en) 3-dimensional in vitro models of mammaliam tissues
PL329644A1 (en) Virtual electroacoustic audiometry for examination of hearing efficiency without any hearing aid, with simulated hearing aid and with a real hearing aid
DE69907153D1 (en) CANCER TREATMENT
AU2002252160A1 (en) Connexin enhances chemotherapy-induced apoptosis in human cancer cells inhibiting tumor cell proliferation
WO2005017189A3 (en) Neural cell assay
AU3182999A (en) Methods of diagnosis and triage using cell activation measures
WO2003014299A3 (en) SPHKs AS MODIFIERS OF THE p53 PATHWAY AND METHODS OF USE
EP1135531A4 (en) Methods for introducing heterologous cells into fish
WO2002099068A3 (en) LCES AS MODIFIERS OF THE p53 PATHWAY AND METHODS OF USE
WO2004099364A3 (en) Multicellular compositions of pluripotent human embryonic stem cells and cancer cells
NO20020666L (en) Use of ginkgo extract
WO2003051905A3 (en) Taojiks as modifiers of the beta-catenin pathway and methods of use
WO2004016739A3 (en) Cell modulation using a cytoskeletal protein
WO2004024879A3 (en) RORs AS MODIFIERS OF THE p21 PATHWAY AND METHODS OF USE
EP1110087A4 (en) Methods for the temporal analysis of programmed cell death in living cells
AU2003237172A1 (en) Cell cultures from animal models of alzheimer's disease for screening and testing drug efficacy
WO1997030354A3 (en) Foetal cell analysis
ATE482979T1 (en) UPS AS BETA-CATENIN PATHWAY MODIFYING SUBSTANCES AND METHODS OF APPLICATION
AU2001290761A1 (en) Methods of diagnosing breast cancer and screening for modulators
WO2005003305A3 (en) Usps as modifiers of the beta catenin pathway and methods of use
WO2003052070A3 (en) Vps41s as modifiers of cell death and methods of use
EP1826560A3 (en) Methods for introducing heterologous cells into fish
WO2004061123A3 (en) Mbcats as modifiers of the beta-catenin pathway and methods of use
WO2003074725A3 (en) MBCATs AS MODIFIERS OF THE BETA-CATENIN PATHWAY AND METHODS OF USE

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

ENP Entry into the national phase

Ref document number: 2375788

Country of ref document: CA

Kind code of ref document: A

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2000934826

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 50560/00

Country of ref document: AU

Ref document number: 200050560

Country of ref document: AU

WWP Wipo information: published in national office

Ref document number: 2000934826

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: JP

WWG Wipo information: grant in national office

Ref document number: 200050560

Country of ref document: AU

WWR Wipo information: refused in national office

Ref document number: 2000934826

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 2000934826

Country of ref document: EP