WO2000052146A3 - Encryption of traits using split gene sequences - Google Patents

Encryption of traits using split gene sequences Download PDF

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Publication number
WO2000052146A3
WO2000052146A3 PCT/US2000/005448 US0005448W WO0052146A3 WO 2000052146 A3 WO2000052146 A3 WO 2000052146A3 US 0005448 W US0005448 W US 0005448W WO 0052146 A3 WO0052146 A3 WO 0052146A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene sequences
methods
split
encryption
organisms
Prior art date
Application number
PCT/US2000/005448
Other languages
French (fr)
Other versions
WO2000052146A2 (en
Inventor
Phillip A Patten
Michael Lassner
Takashi Yamamoto
Brian Carr
Jon E Ness
Ericka R Bermudez
Original Assignee
Maxygen Inc
Phillip A Patten
Michael Lassner
Takashi Yamamoto
Brian Carr
Jon E Ness
Ericka R Bermudez
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Maxygen Inc, Phillip A Patten, Michael Lassner, Takashi Yamamoto, Brian Carr, Jon E Ness, Ericka R Bermudez filed Critical Maxygen Inc
Priority to AU33919/00A priority Critical patent/AU3391900A/en
Priority to EP00912139A priority patent/EP1165757A1/en
Priority to CA002364997A priority patent/CA2364997A1/en
Publication of WO2000052146A2 publication Critical patent/WO2000052146A2/en
Priority to US09/710,686 priority patent/US6531316B1/en
Publication of WO2000052146A3 publication Critical patent/WO2000052146A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Abstract

Methods of unencrypting trait encrypted gene sequences to provide unencrypted RNAs or polypeptides. The invention also relates to methods of encrypting traits including splitting genes between two parental organisms or between a host organism and a vector. The gene sequences are unencrypted when the two parental organisms are mated or when the vector infects the host organism by trans-splicing either the split RNAs or split polypeptides upon expression of the split gene sequences. The invention also includes methods of providing multiple levels of trait encryption and reliable methods of producing hybrid organisms. Additional methods include those related to unencrypting engineered genetic elements to provide polypeptide functions and those directed at recombining non-overlapping gene sequences. The invention also includes integrated systems and various compositions related to the disclosed methods.
PCT/US2000/005448 1999-03-05 2000-03-03 Encryption of traits using split gene sequences WO2000052146A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU33919/00A AU3391900A (en) 1999-03-05 2000-03-03 Encryption of traits using split gene sequences
EP00912139A EP1165757A1 (en) 1999-03-05 2000-03-03 Encryption of traits using split gene sequences
CA002364997A CA2364997A1 (en) 1999-03-05 2000-03-03 Encryption of traits using split gene sequences
US09/710,686 US6531316B1 (en) 1999-03-05 2000-11-09 Encryption of traits using split gene sequences and engineered genetic elements

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US12294399P 1999-03-05 1999-03-05
US60/122,943 1999-03-05
US14229999P 1999-07-02 1999-07-02
US60/142,299 1999-07-02
US16461799P 1999-11-10 1999-11-10
US16461899P 1999-11-10 1999-11-10
US60/164,617 1999-11-10
US60/164,618 1999-11-10

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/005573 Continuation-In-Part WO2000052155A2 (en) 1999-03-05 2000-03-03 Recombination of insertion modified nucleic acids

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US09/710,686 Continuation-In-Part US6531316B1 (en) 1999-03-05 2000-11-09 Encryption of traits using split gene sequences and engineered genetic elements

Publications (2)

Publication Number Publication Date
WO2000052146A2 WO2000052146A2 (en) 2000-09-08
WO2000052146A3 true WO2000052146A3 (en) 2001-07-19

Family

ID=27494459

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2000/005573 WO2000052155A2 (en) 1999-03-05 2000-03-03 Recombination of insertion modified nucleic acids
PCT/US2000/005448 WO2000052146A2 (en) 1999-03-05 2000-03-03 Encryption of traits using split gene sequences

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2000/005573 WO2000052155A2 (en) 1999-03-05 2000-03-03 Recombination of insertion modified nucleic acids

Country Status (5)

Country Link
US (4) US6365377B1 (en)
EP (2) EP1165775A2 (en)
AU (2) AU3391900A (en)
CA (2) CA2362737A1 (en)
WO (2) WO2000052155A2 (en)

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