WO2000042560A3 - Methods for making character strings, polynucleotides and polypeptides - Google Patents

Methods for making character strings, polynucleotides and polypeptides Download PDF

Info

Publication number
WO2000042560A3
WO2000042560A3 PCT/US2000/001202 US0001202W WO0042560A3 WO 2000042560 A3 WO2000042560 A3 WO 2000042560A3 US 0001202 W US0001202 W US 0001202W WO 0042560 A3 WO0042560 A3 WO 0042560A3
Authority
WO
WIPO (PCT)
Prior art keywords
silico
nucleic acid
methods
nucleic acids
character strings
Prior art date
Application number
PCT/US2000/001202
Other languages
French (fr)
Other versions
WO2000042560A2 (en
Inventor
Sergey A Selifonov
Willem P C Stemmer
Claes Gustafsson
Matthew Tobin
Cardayre Stephen Del
Phillip A Patten
Jeremy Minshull
Original Assignee
Maxygen Inc
Sergey A Selifonov
Willem P C Stemmer
Claes Gustafsson
Matthew Tobin
Cardayre Stephen Del
Phillip A Patten
Jeremy Minshull
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/408,393 external-priority patent/US6436675B1/en
Priority claimed from US09/408,392 external-priority patent/US6376246B1/en
Priority to IL14082300A priority Critical patent/IL140823A/en
Priority to EP00902439A priority patent/EP1151409A1/en
Priority to IL13800200A priority patent/IL138002A0/en
Priority to MXPA01001477A priority patent/MXPA01001477A/en
Priority to AU24152/00A priority patent/AU2415200A/en
Priority to KR1020007010375A priority patent/KR20010042037A/en
Priority to EP00909922A priority patent/EP1062614A1/en
Priority to CA2337949A priority patent/CA2337949C/en
Priority to JP2000594066A priority patent/JP4899024B2/en
Priority to CA2721213A priority patent/CA2721213A1/en
Priority to CA002320714A priority patent/CA2320714A1/en
Priority to AU32101/00A priority patent/AU3210100A/en
Application filed by Maxygen Inc, Sergey A Selifonov, Willem P C Stemmer, Claes Gustafsson, Matthew Tobin, Cardayre Stephen Del, Phillip A Patten, Jeremy Minshull filed Critical Maxygen Inc
Priority to JP2000594067A priority patent/JP2002534965A/en
Priority to US09/495,668 priority patent/US6961664B2/en
Priority to US09/539,486 priority patent/US7058515B1/en
Priority to US09/618,579 priority patent/US7024312B1/en
Publication of WO2000042560A2 publication Critical patent/WO2000042560A2/en
Publication of WO2000042560A3 publication Critical patent/WO2000042560A3/en
Priority to US11/075,231 priority patent/US7421347B2/en
Priority to US11/339,090 priority patent/US7620502B2/en
Priority to US11/975,638 priority patent/US7853410B2/en
Priority to US11/982,405 priority patent/US7904249B2/en
Priority to US12/557,463 priority patent/US7957912B2/en
Priority to US13/095,797 priority patent/US20110257892A1/en
Priority to US13/229,228 priority patent/US8457903B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00686—Automatic
    • B01J2219/00689—Automatic using computers

Abstract

'In silico' nucleic acid recombination methods, related integrated systems utilizing genetic operators and libraries made by in silico shuffling methods are provided. An evolutionary process which includes an information manipulation step (application of a genetic algorithm to a character string representing a biopolymer such as a nucleic acid or protein), to create a set of defined information elements (e.g., character strings) which serve as templates for synthesizing physical nucleic acids. The information elements can be placed into a database or otherwise manipulated in silico, e.g., by the recursive application of a GA to the sequences which are produced. Corresponding physical nucleic acids can be subjected to recombination/selection or other diversity generating procedures, with the nucleic acids being deconvoluted (e.g., sequenced or otherwise analyzed) and the overall process repeated, as appropriate, to achieve a desired nucleic acid.
PCT/US2000/001202 1999-01-18 2000-01-18 Methods for making character strings, polynucleotides and polypeptides WO2000042560A2 (en)

Priority Applications (23)

Application Number Priority Date Filing Date Title
JP2000594067A JP2002534965A (en) 1999-01-19 2000-01-18 Methods for producing character strings, polynucleotides and polypeptides having desired characteristics
EP00909922A EP1062614A1 (en) 1999-01-19 2000-01-18 Methods for making character strings, polynucleotides and polypeptides
CA2337949A CA2337949C (en) 1999-01-18 2000-01-18 Methods of populating data structures for use in evolutionary simulations
IL13800200A IL138002A0 (en) 1999-01-19 2000-01-18 Methods for making character strings, polynucleotides and polypeptides having desired characteristics
MXPA01001477A MXPA01001477A (en) 1999-01-18 2000-01-18 Methods of populating data structures for use in evolutionary simulations.
AU24152/00A AU2415200A (en) 1999-01-18 2000-01-18 Methods of populating data structures for use in evolutionary simulations
KR1020007010375A KR20010042037A (en) 1999-01-19 2000-01-18 Methods for making character strings, polynucleotides and polypeptides having desired characteristics
IL14082300A IL140823A (en) 1999-01-19 2000-01-18 Method of populating data structures for use in evolutionary simulations
EP00902439A EP1151409A1 (en) 1999-01-18 2000-01-18 Methods of populating data stuctures for use in evolutionary simulations
JP2000594066A JP4899024B2 (en) 1999-01-18 2000-01-18 How to populate a data structure for use in evolutionary simulation
CA2721213A CA2721213A1 (en) 1999-01-18 2000-01-18 A system for generating labels from sequences of biological molecules
CA002320714A CA2320714A1 (en) 1999-01-19 2000-01-18 Methods for making character strings, polynucleotides and polypeptides having desired characteristics
AU32101/00A AU3210100A (en) 1999-01-19 2000-01-18 Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US09/495,668 US6961664B2 (en) 1999-01-19 2000-02-01 Methods of populating data structures for use in evolutionary simulations
US09/539,486 US7058515B1 (en) 1999-01-19 2000-03-30 Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US09/618,579 US7024312B1 (en) 1999-01-19 2000-07-18 Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US11/075,231 US7421347B2 (en) 1999-01-19 2005-03-07 Identifying oligonucleotides for in vitro recombination
US11/339,090 US7620502B2 (en) 1999-01-19 2006-01-24 Methods for identifying sets of oligonucleotides for use in an in vitro recombination procedure
US11/975,638 US7853410B2 (en) 1999-01-19 2007-10-18 Method for making polynucleotides having desired characteristics
US11/982,405 US7904249B2 (en) 1999-01-19 2007-10-31 Methods for identifying sets of oligonucleotides for use in an in vitro recombination procedures
US12/557,463 US7957912B2 (en) 1999-01-19 2009-09-10 Methods for identifying and producing polypeptides
US13/095,797 US20110257892A1 (en) 1999-01-19 2011-04-27 Methods for identifying sets of oligonucleotides for use in an in vitro recombination procedure
US13/229,228 US8457903B1 (en) 1999-01-19 2011-09-09 Method and/or apparatus for determining codons

Applications Claiming Priority (16)

Application Number Priority Date Filing Date Title
US11644799P 1999-01-19 1999-01-19
US60/116,447 1999-01-19
US11885499P 1999-02-05 1999-02-05
US11881399P 1999-02-05 1999-02-05
US60/118,813 1999-02-05
US60/118,854 1999-02-05
US14104999P 1999-06-24 1999-06-24
US60/141,049 1999-06-24
US09/408,392 1999-09-28
US09/408,393 US6436675B1 (en) 1999-09-28 1999-09-28 Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US09/408,393 1999-09-28
US09/408,392 US6376246B1 (en) 1999-02-05 1999-09-28 Oligonucleotide mediated nucleic acid recombination
US41683799A 1999-10-12 1999-10-12
US41637599A 1999-10-12 1999-10-12
US09/416,375 1999-10-12
US09/416,837 1999-10-12

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
US41637599A Continuation-In-Part 1999-01-18 1999-10-12
US09/484,850 Continuation-In-Part US6368861B1 (en) 1999-01-18 2000-01-18 Oligonucleotide mediated nucleic acid recombination

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US09/494,282 Continuation-In-Part US6917882B2 (en) 1999-01-18 2000-01-18 Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US09/495,668 Continuation-In-Part US6961664B2 (en) 1999-01-19 2000-02-01 Methods of populating data structures for use in evolutionary simulations
US09/539,486 Continuation-In-Part US7058515B1 (en) 1999-01-19 2000-03-30 Methods for making character strings, polynucleotides and polypeptides having desired characteristics

Publications (2)

Publication Number Publication Date
WO2000042560A2 WO2000042560A2 (en) 2000-07-20
WO2000042560A3 true WO2000042560A3 (en) 2000-11-23

Family

ID=27574833

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2000/001202 WO2000042560A2 (en) 1999-01-18 2000-01-18 Methods for making character strings, polynucleotides and polypeptides
PCT/US2000/001203 WO2000042561A2 (en) 1999-01-19 2000-01-18 Oligonucleotide mediated nucleic acid recombination

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/US2000/001203 WO2000042561A2 (en) 1999-01-19 2000-01-18 Oligonucleotide mediated nucleic acid recombination

Country Status (11)

Country Link
US (1) US7058515B1 (en)
EP (6) EP1072010B1 (en)
JP (2) JP2002534965A (en)
AT (1) ATE465459T1 (en)
AU (2) AU3210100A (en)
CA (2) CA2320714A1 (en)
DE (1) DE60044223D1 (en)
DK (1) DK1072010T3 (en)
ES (1) ES2341217T3 (en)
IL (2) IL137868A0 (en)
WO (2) WO2000042560A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9409139B2 (en) 2013-08-05 2016-08-09 Twist Bioscience Corporation De novo synthesized gene libraries
US9512382B2 (en) 2009-10-16 2016-12-06 Bunge Global Innovation, Llc Oil degumming methods

Families Citing this family (207)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6764835B2 (en) 1995-12-07 2004-07-20 Diversa Corporation Saturation mutageneis in directed evolution
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
CA2345203A1 (en) 1998-10-07 2000-04-13 Maxygen Inc. Dna shuffling to produce nucleic acids for mycotoxin detoxification
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US20070065838A1 (en) 1999-01-19 2007-03-22 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US7873477B1 (en) 2001-08-21 2011-01-18 Codexis Mayflower Holdings, Llc Method and system using systematically varied data libraries
US7024312B1 (en) * 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US8457903B1 (en) * 1999-01-19 2013-06-04 Codexis Mayflower Holdings, Llc Method and/or apparatus for determining codons
US7702464B1 (en) * 2001-08-21 2010-04-20 Maxygen, Inc. Method and apparatus for codon determining
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
US7430477B2 (en) * 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
AU1456101A (en) 1999-11-03 2001-05-14 Maxygen, Inc. Antibody diversity generation
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
AU782580B2 (en) 2000-01-10 2005-08-11 Maxygen, Inc. G-CSF conjugates
DK1257295T3 (en) 2000-02-11 2009-08-10 Bayer Healthcare Llc Factor VII or VIIA-like molecules
AU2001241939A1 (en) * 2000-02-28 2001-09-12 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
AU4964101A (en) * 2000-03-30 2001-10-15 Maxygen Inc In silico cross-over site selection
FR2810339B1 (en) * 2000-06-14 2004-12-10 Hoechst Marion Roussel Inc COMBINATORIAL BANKS IMPROVED BY RECOMBINATION IN YEAST AND METHOD OF ANALYSIS
EP1360290A2 (en) 2000-06-23 2003-11-12 Maxygen, Inc. Co-stimulatory molecules
US6994963B1 (en) * 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
WO2002006469A2 (en) * 2000-07-18 2002-01-24 Enchira Biotechnology Corporation Methods of ligation mediated chimeragenesis utilizing populations of scaffold and donor nucleic acids
WO2005012515A2 (en) 2003-04-29 2005-02-10 Pioneer Hi-Bred International, Inc. Novel glyphosate-n-acetyltransferase (gat) genes
SK5222003A3 (en) 2000-10-30 2004-12-01 Maxygen Inc Novel glyphosate N-acetyltransferase (GAT) genes
JP2004533228A (en) * 2001-01-19 2004-11-04 エジー バイオサイエンシーズ， インコーポレイテッド Computer-based assembly of a polynucleotide encoding a target polypeptide
WO2002079502A1 (en) * 2001-03-28 2002-10-10 The University Of Queensland A method for nucleic acid sequence analysis
AU2002368549A1 (en) * 2001-05-09 2008-10-23 Maxygen, Inc. Methods, systems, and software for regulated oligonucleotide-mediated recombination
AU2002314997A1 (en) * 2001-06-05 2002-12-16 Gorilla Genomics, Inc. Methods for low background cloning of dna using long oligonucleotides
AU2002333269A1 (en) * 2001-07-23 2003-02-17 Dsm Ip Assets B.V. Process for preparing variant polynucleotides
SE0102812D0 (en) * 2001-08-23 2001-08-23 Jan Biro New conversion method
EP1288835A1 (en) * 2001-08-31 2003-03-05 Hitachi, Ltd. A method for defining gene hunting studies
US20050084907A1 (en) 2002-03-01 2005-04-21 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
EP2278509B1 (en) 2002-03-01 2014-11-19 Codexis Mayflower Holdings, LLC Methods, systems, and software for identifying functional biomolecules
US20040132101A1 (en) 2002-09-27 2004-07-08 Xencor Optimized Fc variants and methods for their generation
US7747391B2 (en) 2002-03-01 2010-06-29 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
US7657380B2 (en) 2003-12-04 2010-02-02 Xencor, Inc. Methods of generating variant antibodies with increased host string content
JP4851687B2 (en) 2002-03-09 2012-01-11 マキシジェン， インコーポレイテッド Crossover optimization for directed evolution
US9321832B2 (en) 2002-06-28 2016-04-26 Domantis Limited Ligand
DE10233022B4 (en) * 2002-07-20 2004-09-16 Zinn, Peter, Dr. Process for solving tasks in adaptive chemistry
EP2275536A1 (en) 2002-08-06 2011-01-19 Verdia, Inc. AP1 amine oxidase variants
ES2562177T3 (en) 2002-09-27 2016-03-02 Xencor Inc. Optimized Fc variants and methods for their generation
US7871799B2 (en) * 2002-11-22 2011-01-18 Lawrence Livermore National Security, Llc Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions
DE10260805A1 (en) * 2002-12-23 2004-07-22 Geneart Gmbh Method and device for optimizing a nucleotide sequence for expression of a protein
EP2390262A1 (en) * 2003-05-16 2011-11-30 Intermune, Inc. Synthetic chemokine receptor ligands and methods of use thereof
US7524664B2 (en) 2003-06-17 2009-04-28 California Institute Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome P450
US7580909B2 (en) * 2003-08-26 2009-08-25 Northrop Grumman Corporation Visual representation tool for structured arguments
US8883147B2 (en) 2004-10-21 2014-11-11 Xencor, Inc. Immunoglobulins insertions, deletions, and substitutions
US8399618B2 (en) 2004-10-21 2013-03-19 Xencor, Inc. Immunoglobulin insertions, deletions, and substitutions
JP4843778B2 (en) * 2003-10-08 2011-12-21 独立行政法人産業技術総合研究所 siRNA RNAi effect calculation device
PT2184360E (en) 2004-02-25 2015-12-02 Pioneer Hi Bred Int Novel bacillus thuringiensis crystal polypeptides, polynucleotides, and compositions thereof
WO2005092925A2 (en) 2004-03-24 2005-10-06 Xencor, Inc. Immunoglobulin variants outside the fc region
WO2005123929A2 (en) 2004-06-09 2005-12-29 Pioneer Hi-Bred International, Inc. Plastid transit peptides
EP2325207B1 (en) 2004-11-12 2017-03-15 Xencor, Inc. FC variants with altered binding to FCRN
JP2006158287A (en) * 2004-12-07 2006-06-22 Hitachi Software Eng Co Ltd Method and device for designing and displaying primer
JP4937138B2 (en) 2005-01-05 2012-05-23 エフ−シュタール・ビオテヒノロギシェ・フォルシュングス−ウント・エントヴィックルングスゲゼルシャフト・ミット・ベシュレンクテル・ハフツング Synthetic immunoglobulin domains with binding properties designed in a region of the molecule that is different from the complementarity-determining region
EP1844144B1 (en) * 2005-01-10 2010-04-14 MedImmune Limited Method of mutagenesis
GB0500417D0 (en) 2005-01-10 2005-02-16 Cambridge Antibody Tech Method of mutagenesis
GB0500703D0 (en) * 2005-01-14 2005-02-23 Bioinvent Int Ab Molecular biology method
AU2006208199B2 (en) 2005-01-25 2012-06-07 Cell Therapeutics, Inc. Conjugates of biologically active proteins having a modified in vivo half-life
US8715988B2 (en) 2005-03-28 2014-05-06 California Institute Of Technology Alkane oxidation by modified hydroxylases
ES2843401T3 (en) 2005-10-28 2021-07-16 Dow Agrosciences Llc New herbicide resistance genes
US20070161031A1 (en) * 2005-12-16 2007-07-12 The Board Of Trustees Of The Leland Stanford Junior University Functional arrays for high throughput characterization of gene expression regulatory elements
EP2322643B1 (en) 2006-03-07 2014-07-09 Cargill, Incorporated Aldolases, nucleic acids encoding them and methods for making and using them
CA2649296A1 (en) 2006-04-13 2007-10-25 Peptimmune, Inc. Methods for designing and synthesizing directed sequence polymer compositions via the directed expansion of epitope permeability
AT503861B1 (en) 2006-07-05 2008-06-15 F Star Biotech Forsch & Entw METHOD FOR MANIPULATING T-CELL RECEPTORS
AT503889B1 (en) 2006-07-05 2011-12-15 Star Biotechnologische Forschungs Und Entwicklungsges M B H F MULTIVALENT IMMUNE LOBULINE
AT503902B1 (en) 2006-07-05 2008-06-15 F Star Biotech Forsch & Entw METHOD FOR MANIPULATING IMMUNE LOBULINS
EP2069513A4 (en) 2006-08-04 2012-03-21 California Inst Of Techn Methods and systems for selective fluorination of organic molecules
US8252559B2 (en) 2006-08-04 2012-08-28 The California Institute Of Technology Methods and systems for selective fluorination of organic molecules
US20090118130A1 (en) 2007-02-12 2009-05-07 Codexis, Inc. Structure-activity relationships
WO2008112127A2 (en) * 2007-03-08 2008-09-18 Switchgear Genomics Functional arrays for high throughput characterization of regulatory elements in untranslated regions of genes
CA2686835C (en) 2007-05-09 2020-04-21 Dow Agrosciences Llc Novel herbicide resistance genes
WO2009000006A1 (en) 2007-06-26 2008-12-31 F-Star Biotechnologische Forschungs- Und Entwicklungsges.M.B.H. Display of binding agents
US9533037B2 (en) 2007-10-16 2017-01-03 Declion Holdings Llc Methods for designing and preparing vaccines comprising directed sequence polymer compositions via the directed expansion of epitopes
US7682809B2 (en) 2008-01-11 2010-03-23 Agilent Technologies, Inc. Direct ATP release sequencing
US8768871B2 (en) 2008-02-12 2014-07-01 Codexis, Inc. Method of generating an optimized, diverse population of variants
DK2250595T3 (en) 2008-02-12 2017-09-04 Codexis Inc PROCEDURE FOR SELECTING AN OPTIMIZED VARIETY OF VARIETIES
EP2113255A1 (en) 2008-05-02 2009-11-04 f-star Biotechnologische Forschungs- und Entwicklungsges.m.b.H. Cytotoxic immunoglobulin
CA2990650C (en) 2008-05-23 2021-02-09 Pioneer Hi-Bred International, Inc. Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
US8383346B2 (en) 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
WO2010025287A2 (en) 2008-08-27 2010-03-04 Codexis, Inc. Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
SI2329013T1 (en) 2008-08-27 2016-03-31 Codexis, Inc. Ketoreductase polypeptides for the production of a 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
WO2010030171A1 (en) * 2008-09-11 2010-03-18 Keygene N.V. Method for diagnostic marker development
KR102025502B1 (en) 2008-09-26 2019-09-25 토카겐 인크. Gene therapy vectors and cytosine deaminases
US8731843B2 (en) * 2009-02-03 2014-05-20 Complete Genomics, Inc. Oligomer sequences mapping
US8615365B2 (en) * 2009-02-03 2013-12-24 Complete Genomics, Inc. Oligomer sequences mapping
EP2393944A4 (en) * 2009-02-03 2013-11-27 Complete Genomics Inc Indexing a reference sequence for oligomer sequence mapping
CN102459315B (en) 2009-04-17 2016-03-02 陶氏益农公司 DIG-3 insecticidal cry toxins
EP2430441B1 (en) * 2009-04-29 2018-06-13 Complete Genomics, Inc. Method and system for calling variations in a sample polynucleotide sequence with respect to a reference polynucleotide sequence
US8815779B2 (en) * 2009-09-16 2014-08-26 SwitchGear Genomics, Inc. Transcription biomarkers of biological responses and methods
SG179197A1 (en) 2009-09-18 2012-04-27 Codexis Inc Reduced codon mutagenesis
US9234208B1 (en) 2010-05-10 2016-01-12 Dow Agrosciences Llc DIG-13 insecticidal cry toxins
CA2805941A1 (en) 2010-08-13 2012-02-16 Pioneer Hi-Bred International, Inc. Methods and compositions for targeting sequences of interest to the chloroplast
DK2606131T3 (en) 2010-08-20 2017-06-19 Codexis Inc Use of proteins from glycoside hydrolase 61 family for cellulose processing
US9322027B2 (en) 2010-08-20 2016-04-26 Shell Oil Company Expression constructs comprising fungal promoters
EP2691517A4 (en) 2011-03-30 2015-04-22 Codexis Inc Pentose fermentation by a recombinant microorganism
BR112013033614A2 (en) 2011-06-30 2018-05-29 Codexis Inc pentose fermentation by recombinant microorganism
US9322007B2 (en) 2011-07-22 2016-04-26 The California Institute Of Technology Stable fungal Cel6 enzyme variants
DK2748317T3 (en) 2011-08-22 2017-07-17 Codexis Inc GH61 glycoside hydrolase protein variants and cofactors that enhance GH61 activity
EP2760997A4 (en) 2011-09-30 2015-02-11 Codexis Inc Fungal proteases
EP2794871A4 (en) 2011-12-20 2015-12-30 Codexis Inc Endoglucanase 1b (eg1b) variants
AP2014007885A0 (en) 2012-02-01 2014-08-31 Dow Agrosciences Llc Novel class of glyphosate resistance genes
CA2872096A1 (en) 2012-05-04 2013-11-07 E. I. Du Pont De Nemours And Company Compositions and methods comprising sequences having meganuclease activity
WO2013188305A2 (en) 2012-06-11 2013-12-19 Codexis, Inc. Fungal beta-xylosidase variants
AR092482A1 (en) 2012-09-07 2015-04-22 Dow Agrosciences Llc ENRICHMENT OF THE CLASSIFICATION OF FLUORESCENCE ACTIVATED CELLS (FACS) TO GENERATE PLANTS
EP2912169A4 (en) 2012-10-29 2016-11-23 Univ Rochester Artemisinin derivatives, methods for their preparation and their use as antimalarial agents
AU2013348272A1 (en) 2012-11-20 2015-05-14 Shell Internationale Research Maatschappij B.V. Pentose fermentation by a recombinant microorganism
KR101600902B1 (en) * 2012-12-11 2016-03-10 주식회사 셀레믹스 Method of synthesis of gene library using codon randomization and mutagenesis
KR102215219B1 (en) 2013-01-31 2021-02-16 코덱시스, 인코포레이티드 Methods, systems, and software for identifying bio-molecules using models of multiplicative form
WO2014153234A1 (en) 2013-03-14 2014-09-25 Pioneer Hi-Bred International, Inc. Compositions having dicamba decarboxylase activity and methods of use
WO2014153242A1 (en) 2013-03-14 2014-09-25 Pioneer Hi-Bred International, Inc. Compositions having dicamba decarboxylase activity and methods of use
EP2971000A4 (en) 2013-03-15 2016-11-23 Pioneer Hi Bred Int Phi-4 polypeptides and methods for their use
PE20151775A1 (en) 2013-04-18 2015-12-02 Codexis Inc POLYPEPTIDES OF PHENYLALANINE AMMONIA LIASA MODIFIED BY GENETIC ENGINEERING
EA030896B1 (en) 2013-08-16 2018-10-31 Пайонир Хай-Бред Интернэшнл, Инк. Insecticidal proteins and methods for their use
CN105637099B (en) 2013-08-23 2020-05-19 深圳华大智造科技有限公司 Long fragment de novo assembly using short reads
BR122021005579B1 (en) 2013-09-13 2022-11-29 Pioneer Hi-Bred International, Inc DNA CONSTRUCTION, METHOD FOR OBTAINING A TRANSGENIC PLANT, FUSION PROTEIN, METHOD FOR CONTROLLING AN INSECT PEST POPULATION, METHOD FOR INHIBITING THE GROWTH OR KILLING AN INSECT PEST
SG11201601695WA (en) 2013-09-27 2016-04-28 Codexis Inc Automated screening of enzyme variants
WO2015073555A1 (en) 2013-11-13 2015-05-21 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
WO2015100277A2 (en) 2013-12-23 2015-07-02 University Of Rochester Methods and compositions for ribosomal synthesis of macrocyclic peptides
CN117903266A (en) 2014-02-07 2024-04-19 先锋国际良种公司 Insecticidal proteins and methods of use thereof
CN114763376A (en) 2014-02-07 2022-07-19 先锋国际良种公司 Insecticidal proteins and methods of use thereof
AR099800A1 (en) 2014-03-21 2016-08-17 Agrigenetics Inc CRY1D TO CONTROL THE CORN WORM
CA2943432A1 (en) 2014-04-16 2015-10-22 Codexis, Inc. Engineered tyrosine ammonia lyase
EP3167052B1 (en) 2014-07-09 2020-01-01 Codexis, Inc. P450-bm3 variants with improved activity
BR112017007932A2 (en) 2014-10-16 2018-01-23 Du Pont insecticide proteins and methods for their use
SG10202012428PA (en) 2014-11-25 2021-01-28 Codexis Inc Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
DK3237621T3 (en) 2014-12-22 2023-06-19 Codexis Inc VARIANTS OF HUMAN ALPHA-GALACTOSIDASE
CN114213511A (en) 2014-12-30 2022-03-22 美国陶氏益农公司 Modified Cry1Ca toxins useful for controlling insect pests
CA2975855A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016130412A1 (en) 2015-02-10 2016-08-18 Codexis, Inc. Ketoreductase polypeptides for the synthesis of chiral compounds
MX2017011525A (en) 2015-03-11 2018-01-30 Pioneer Hi Bred Int Insecticidal combinations of pip-72 and methods of use.
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
NZ736220A (en) 2015-05-07 2024-03-22 Codexis Inc Penicillin-g acylases
CA2985198A1 (en) 2015-05-19 2016-11-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US9683220B2 (en) 2015-07-07 2017-06-20 Codexis, Inc. P450-BM3 variants with improved activity
MX2018001523A (en) 2015-08-06 2018-03-15 Pioneer Hi Bred Int Plant derived insecticidal proteins and methods for their use.
CN108026149B (en) 2015-08-17 2022-04-29 美国陶氏益农公司 Engineered CRY6A insecticidal proteins
US10844373B2 (en) 2015-09-18 2020-11-24 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
KR20180058772A (en) 2015-09-22 2018-06-01 트위스트 바이오사이언스 코포레이션 Flexible substrate for nucleic acid synthesis
US9895673B2 (en) 2015-12-01 2018-02-20 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
WO2017105987A1 (en) 2015-12-18 2017-06-22 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2017192560A1 (en) 2016-05-04 2017-11-09 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3452587B1 (en) 2016-05-05 2021-09-08 Codexis, Inc. Penicillin-g acylases
WO2017213758A1 (en) 2016-06-09 2017-12-14 Codexis, Inc. Biocatalysts and methods for hydroxylation of chemical compounds
BR112018075932A2 (en) 2016-06-15 2019-10-01 Codexis Inc unnaturally occurring beta-glycosidase variant, recombinant polynucleotide, vector, host cell, methods for producing at least one unnaturally occurring beta-glucosidase variant for conversion of a glycosyl group-acceptor substrate to a beta-glycosylated product and for transglycosylation of a substrate, and, composition.
EP3954202A1 (en) 2016-07-01 2022-02-16 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
CA3034769A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
CN109863162B (en) 2016-08-26 2023-03-17 科德克希思公司 Engineered imine reductases and methods for reductive amination of ketone and amine compounds
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
US11021716B2 (en) 2016-11-01 2021-06-01 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CA3044404A1 (en) 2016-12-14 2018-06-21 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
HUE049372T2 (en) 2016-12-14 2020-09-28 Biological Res Centre Mutagenizing intracellular nucleic acids
KR102514213B1 (en) 2016-12-16 2023-03-27 트위스트 바이오사이언스 코포레이션 Immune synaptic variant library and its synthesis
WO2018118811A1 (en) 2016-12-22 2018-06-28 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CA3048475A1 (en) 2017-01-05 2018-07-12 Codexis, Inc. Penicillin-g acylases
KR102606350B1 (en) 2017-02-03 2023-11-23 코덱시스, 인코포레이티드 Engineered glycosyltransferase and steviol glycoside glucosylation method
WO2018148001A1 (en) 2017-02-08 2018-08-16 Pioneer Hi-Bred International Inc Insecticidal combinations of plant derived insecticidal proteins and methods for their use
SG11201906880VA (en) 2017-02-13 2019-08-27 Codexis Inc Engineered phenylalanine ammonia lyase polypeptides
EP3586255A4 (en) 2017-02-22 2021-03-31 Twist Bioscience Corporation Nucleic acid based data storage
CN110913865A (en) 2017-03-15 2020-03-24 特韦斯特生物科学公司 Library of variants of immune synapses and synthesis thereof
CA3061133A1 (en) 2017-04-27 2018-11-01 Codexis, Inc. Ketoreductase polypeptides and polynucleotides
EP3635101B1 (en) 2017-05-08 2021-08-18 Codexis, Inc. Engineered ligase variants
MX2019013321A (en) 2017-05-11 2020-02-10 Pioneer Hi Bred Int Insecticidal proteins and methods for their use.
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
SG11201912057RA (en) 2017-06-12 2020-01-30 Twist Bioscience Corp Methods for seamless nucleic acid assembly
CN111032682B (en) 2017-06-14 2024-04-12 科德克希思公司 Engineered transaminase polypeptides for industrial biocatalysis
CA3064577A1 (en) 2017-06-27 2019-01-03 Codexis, Inc. Penicillin-g acylases
CN111417724A (en) 2017-06-30 2020-07-14 科德克希思公司 T7RNA polymerase variants
CN111032863A (en) 2017-06-30 2020-04-17 科德克希思公司 T7RNA polymerase variants
US11634400B2 (en) 2017-08-19 2023-04-25 University Of Rochester Micheliolide derivatives, methods for their preparation and their use as anticancer and antiinflammatory agents
CN111566125A (en) 2017-09-11 2020-08-21 特韦斯特生物科学公司 GPCR binding proteins and synthesis thereof
US10894242B2 (en) 2017-10-20 2021-01-19 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
JP2021502068A (en) 2017-11-07 2021-01-28 コデクシス， インコーポレイテッド Transglutaminase variant
CN111699250B (en) 2017-12-13 2024-01-09 科德克希思公司 Carboxylesterase polypeptides for amide coupling
KR20200106067A (en) 2018-01-04 2020-09-10 트위스트 바이오사이언스 코포레이션 DNA-based digital information storage
CN108341342B (en) * 2018-01-23 2019-04-12 浙江大学 The overhead crane model construction of SVM method of prominent ring crossover operation RNA-GA
WO2019222706A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
CA3102968A1 (en) 2018-06-12 2019-12-19 Codexis, Inc. Engineered tyrosine ammonia lyase
WO2020014047A1 (en) 2018-07-09 2020-01-16 Codexis, Inc. Engineered purine nucleoside phosphorylase variant enzymes
KR20210032417A (en) 2018-07-09 2021-03-24 코덱시스, 인코포레이티드 Engineered deoxyribose-phosphate aldolase
WO2020014046A1 (en) 2018-07-09 2020-01-16 Codexis, Inc. Engineered phosphopentomutase variant enzymes
CN112673091A (en) 2018-07-09 2021-04-16 科德克希思公司 Engineered pantothenate kinase variant enzymes
JP2021530982A (en) 2018-07-09 2021-11-18 コデクシス， インコーポレイテッド Manipulated galactose oxidase variant enzyme
WO2020013951A1 (en) 2018-07-12 2020-01-16 Codexis, Inc. Engineered phenylalanine ammonia lyase polypeptides
JP2021532757A (en) 2018-07-30 2021-12-02 コデクシス， インコーポレイテッド Engineered Glycosyltransferase and Steviol Glycoside Glycosylation Methods
CN112585687A (en) * 2018-08-15 2021-03-30 齐默尔根公司 Bioaccessible predictive tool with biological sequence selection
MX2021004925A (en) 2018-10-29 2021-06-15 Codexis Inc Engineered dna polymerase variants.
SG11202105668PA (en) 2018-12-14 2021-06-29 Codexis Inc Engineered tyrosine ammonia lyase
EA202191735A1 (en) 2018-12-20 2021-11-24 Кодексис, Инк. HUMAN ALPHA-GALACTOSIDASE OPTIONS
CN113785057A (en) 2019-02-26 2021-12-10 特韦斯特生物科学公司 Variant nucleic acid libraries for antibody optimization
CA3131689A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for glp1 receptor
US11332738B2 (en) 2019-06-21 2022-05-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
JP2023521772A (en) 2020-04-10 2023-05-25 コデクシス， インコーポレイテッド Engineered transaminase polypeptide
US20230235352A1 (en) 2020-07-14 2023-07-27 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2022047264A1 (en) 2020-08-28 2022-03-03 Codexis, Inc. Engineered amylase variants
AU2021334371A1 (en) 2020-08-28 2023-03-09 Codexis, Inc. Engineered protease variants
IL303470A (en) 2020-12-18 2023-08-01 Codexis Inc Engineered uridine phosphorylase variant enzymes
EP4314261A1 (en) 2021-04-02 2024-02-07 Codexis, Inc. Engineered acetate kinase variant enzymes
CN117120600A (en) 2021-04-02 2023-11-24 科德克希思公司 Engineered adenylate kinase variant enzymes
CA3214975A1 (en) 2021-04-02 2022-10-06 Codexis, Inc. Engineered cyclic gmp-amp synthase (cgas) variant enzymes
CN117120599A (en) 2021-04-02 2023-11-24 科德克希思公司 Engineered guanylate kinase variant enzymes
EP4320148A1 (en) * 2021-04-09 2024-02-14 Abterra Biosciences, Inc. Method for antibody identification from protein mixtures
WO2023173084A1 (en) 2022-03-11 2023-09-14 University Of Rochester Cyclopeptibodies and uses thereof
WO2024040020A1 (en) 2022-08-15 2024-02-22 Absci Corporation Quantitative affinity activity specific cell enrichment
WO2024047178A1 (en) 2022-09-02 2024-03-07 Eleven Therapeutics Ltd A method for simultaneous synthesis of a plurality of oligonucleotides

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997020078A1 (en) * 1995-11-30 1997-06-05 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
WO1997035966A1 (en) * 1996-03-25 1997-10-02 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
WO1998042832A1 (en) * 1997-03-25 1998-10-01 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers

Family Cites Families (88)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4994379A (en) 1983-03-09 1991-02-19 Cetus Corporation Modified signal peptides
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4959312A (en) 1985-05-31 1990-09-25 The University Of Tennessee Research Corporation Full spectrum mutagenesis
US5866363A (en) 1985-08-28 1999-02-02 Pieczenik; George Method and means for sorting and identifying biological information
US5824469A (en) 1986-07-17 1998-10-20 University Of Washington Method for producing novel DNA sequences with biological activity
US5447839A (en) 1988-09-09 1995-09-05 Hoffmann-La Roche Inc. Detection of human papillomavirus by the polymerase chain reaction
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
FR2641793B1 (en) 1988-12-26 1993-10-01 Setratech METHOD OF IN VIVO RECOMBINATION OF DNA SEQUENCES HAVING BASIC MATCHING
US5043272A (en) 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5023171A (en) * 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
US5071743A (en) * 1989-10-27 1991-12-10 Her Majesty The Queen In Right Of Canada, As Represented By The National Research Council Of Canada Process for conducting site-directed mutagenesis
US5264563A (en) 1990-08-24 1993-11-23 Ixsys Inc. Process for synthesizing oligonucleotides with random codons
WO1992003917A1 (en) 1990-08-29 1992-03-19 Genpharm International Homologous recombination in mammalian cells
IL99553A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing oligonucleotides linked to expression elements,a kit for the preparation of vectors useful for the expression of a diverse population of random peptides and methods utilizing the same
US5512463A (en) 1991-04-26 1996-04-30 Eli Lilly And Company Enzymatic inverse polymerase chain reaction library mutagenesis
US5675253A (en) 1991-11-20 1997-10-07 Auburn International, Inc. Partial least square regression techniques in obtaining measurements of one or more polymer properties with an on-line nmr system
US5650722A (en) 1991-11-20 1997-07-22 Auburn International, Inc. Using resin age factor to obtain measurements of improved accuracy of one or more polymer properties with an on-line NMR system
US5519319A (en) 1991-11-20 1996-05-21 Auburn International, Inc. Obtaining measurements of improved accuracy of one or more polymer properties with an on-line NMR system
US5869644A (en) 1992-04-15 1999-02-09 The Johns Hopkins University Synthesis of diverse and useful collections of oligonucleotidies
US5319308A (en) 1992-10-13 1994-06-07 Auburn International, Inc. Real time magnetic resonance analysis with non-linear regression means
US5408181A (en) 1993-08-30 1995-04-18 Auburn International, Inc. NMR system for measuring polymer properties
US5426039A (en) 1993-09-08 1995-06-20 Bio-Rad Laboratories, Inc. Direct molecular cloning of primer extended DNA containing an alkane diol
PT733059E (en) 1993-12-09 2001-03-30 Univ Jefferson COMPOUNDS AND METHODS FOR LOCAL MUTACOES IN EUCARIOTIC CELLS
JP3082549B2 (en) 1993-12-27 2000-08-28 株式会社村田製作所 Method for producing ceramic green sheet with supporting film
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US5521077A (en) 1994-04-28 1996-05-28 The Leland Stanford Junior University Method of generating multiple protein variants and populations of protein variants prepared thereby
US5580730A (en) 1994-08-19 1996-12-03 Olympus America, Inc. Enzyme digestion method for the detection of amplified DNA
US5717085A (en) 1994-11-23 1998-02-10 Terrapin Technologies, Inc. Process for preparing codon amidites
US5514588A (en) 1994-12-13 1996-05-07 Exxon Research And Engineering Company Surfactant-nutrients for bioremediation of hydrocarbon contaminated soils and water
WO1996020947A1 (en) 1994-12-30 1996-07-11 Chiron Corporation Method for the controlled synthesis of polynucleotide mixtures which encode desired mixtures of peptides
KR19990008000A (en) 1995-04-24 1999-01-25 로버트 에스. 화이트 헤드 How to create a new metabolic pathway and screen it
FI104465B (en) 1995-06-14 2000-02-15 Valio Oy Protein hydrolyzates for the treatment and prevention of allergies and their preparation and use
US6004788A (en) 1995-07-18 1999-12-21 Diversa Corporation Enzyme kits and libraries
US6030779A (en) 1995-07-18 2000-02-29 Diversa Corporation Screening for novel bioactivities
US6057103A (en) 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
US5958672A (en) 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
DE69620766T2 (en) 1995-08-11 2004-11-18 Novozymes A/S METHOD FOR THE PRODUCTION OF POLYPEPTIDE COMBS
US5962258A (en) 1995-08-23 1999-10-05 Diversa Corporation Carboxymethyl cellulase fromthermotoga maritima
US5756316A (en) 1995-11-02 1998-05-26 Genencor International, Inc. Molecular cloning by multimerization of plasmids
US5965408A (en) * 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US5962283A (en) 1995-12-07 1999-10-05 Diversa Corporation Transminases and amnotransferases
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US5814473A (en) 1996-02-09 1998-09-29 Diversa Corporation Transaminases and aminotransferases
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
CN1210557A (en) 1996-01-10 1999-03-10 诺沃挪第克公司 Method for in vivo production of mutant library in cells
US5942430A (en) 1996-02-16 1999-08-24 Diversa Corporation Esterases
US5958751A (en) 1996-03-08 1999-09-28 Diversa Corporation α-galactosidase
AU2074397A (en) 1996-03-15 1997-10-01 Amway Corporation Powder detergent composition having improved solubility
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US5783431A (en) 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US5760012A (en) 1996-05-01 1998-06-02 Thomas Jefferson University Methods and compounds for curing diseases caused by mutations
US5789228A (en) 1996-05-22 1998-08-04 Diversa Corporation Endoglucanases
US5877001A (en) 1996-06-17 1999-03-02 Diverso Corporation Amidase
US5763239A (en) 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
EP0949930B1 (en) 1996-06-19 2004-10-06 Diversa Corporation Thermostable phosphatases
US5939300A (en) 1996-07-03 1999-08-17 Diversa Corporation Catalases
AU4597197A (en) 1996-09-27 1998-04-17 Introgene B.V. Methods for optimization of gene therapy by recursive sequence shuffling and selection
JP2001504325A (en) 1996-09-27 2001-04-03 マキシジェン，インコーポレイテッド A method for optimization of gene therapy by recursive sequence shuffling and selection
ATE295850T1 (en) 1996-12-06 2005-06-15 Diversa Corp GLYCOSIDASE ENZYMES
JPH10185925A (en) 1996-12-20 1998-07-14 Seibutsu Bunshi Kogaku Kenkyusho:Kk Structural alignment method for double dynamic programming
WO1998028416A1 (en) 1996-12-20 1998-07-02 Novo Nordisk A/S In vivo recombination
AU743305C (en) 1997-01-17 2006-03-30 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
WO1998036080A1 (en) 1997-02-13 1998-08-20 The Dow Chemical Company Recombinant haloaliphatic dehalogenases
WO1998041622A1 (en) 1997-03-18 1998-09-24 Novo Nordisk A/S Method for constructing a library using dna shuffling
DK0973940T3 (en) 1997-03-18 2008-10-20 Novozymes As An in vitro method for constructing a DNA library
NZ337910A (en) 1997-03-18 2001-10-26 Novo Nordisk As Shuffling of heterologous DNA sequences using conserved regions
US5948653A (en) 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
US6188965B1 (en) 1997-04-11 2001-02-13 California Institute Of Technology Apparatus and method for automated protein design
PL330759A1 (en) 1997-04-22 1999-05-24 Gist Brocades Bv Method of obtaining deacylated cephalosporins by fermentation process
EP0979311A1 (en) * 1997-04-30 2000-02-16 Of The University Of Minnesota Regents $i(IN VIVO) USE OF RECOMBINAGENIC OLIGONUCLEOBASES TO CORRECT GENETIC LESIONS IN HEPATOCYTES
GB9712512D0 (en) 1997-06-16 1997-08-20 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
US5948666A (en) 1997-08-06 1999-09-07 Diversa Corporation Isolation and identification of polymerases
US5876997A (en) 1997-08-13 1999-03-02 Diversa Corporation Phytase
US6610528B1 (en) 1997-08-26 2003-08-26 Diversa Corporation Microbial enrichment using a container having a plurality of solid support particles
US6846627B2 (en) 1997-10-15 2005-01-25 Diversa Corporation Screening for novel compounds which regulate biological interactions
WO1999021979A1 (en) 1997-10-28 1999-05-06 Maxygen, Inc. Human papillomavirus vectors
US6596539B1 (en) 1997-10-31 2003-07-22 Maxygen, Inc. Modification of virus tropism and host range by viral genome shuffling
AU2674199A (en) 1998-02-11 1999-08-30 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
EP1054973A1 (en) 1998-02-11 2000-11-29 Maxygen, Inc. Antigen library immunization
US5914245A (en) 1998-04-20 1999-06-22 Kairos Scientific Inc. Solid phase enzyme kinetics screening in microcolonies
US6403312B1 (en) 1998-10-16 2002-06-11 Xencor Protein design automatic for protein libraries
US9922588B2 (en) 2014-01-29 2018-03-20 Sharp Kabushiki Kaisha Image display device
US10336999B2 (en) 2015-05-29 2019-07-02 Zymergen Inc. Compositions and methods for activating silent gene clusters

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997020078A1 (en) * 1995-11-30 1997-06-05 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
WO1997035966A1 (en) * 1996-03-25 1997-10-02 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
WO1998042832A1 (en) * 1997-03-25 1998-10-01 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
FENGZHU SUN: "Modeling DNA Shuffling", PROCEEDINGS OF THE SECOND ANNUAL INTERNATIONAL CONFERENCE ON COMPUTATIONAL MOLECULAR BIOLOGY, 22 March 1998 (1998-03-22) - 25 March 1998 (1998-03-25), NY, USA, pages 251 - 257, XP002145606 *
GIVER L ET AL: "COMBINATORAL PROTEIN DESIGN BY IN VITRO RECOMBINATION", CURRENT OPINION IN CHEMICAL BIOLOGY,GB,CURRENT BIOLOGY LTD, LONDON, vol. 2, no. 3, June 1998 (1998-06-01), pages 335 - 338, XP000892913, ISSN: 1367-5931 *
PATTEN P A ET AL: "APPLICATIONS OF DNA SHUFFLING TO PHARMACEUTICALS AND VACCINES", CURRENT OPINION IN BIOTECHNOLOGY,GB,LONDON, vol. 8, 1997, pages 724 - 733, XP002916609, ISSN: 0958-1669 *
STEMMER W P C: "Rapid evolution of a protein in vitro by DNA shuffling", NATURE, 4 August 1994 (1994-08-04), XP002082182 *
VENKATASUBRAMANIAN V ET AL: "EVOLUTIONARY DESIGN OF MOLECULES WITH DESIRED PROPERTIES USING THE GENETIC ALGORITHM", JOURNAL OF CHEMICAL INFORMATION AND COMPUTER SCIENCES,US,AMERICAN CHEMICAL SOCIETY, COLOMBUS,OHIO, vol. 35, no. 2, 1 March 1995 (1995-03-01), pages 188 - 195, XP000576025, ISSN: 0095-2338 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9512382B2 (en) 2009-10-16 2016-12-06 Bunge Global Innovation, Llc Oil degumming methods
US9409139B2 (en) 2013-08-05 2016-08-09 Twist Bioscience Corporation De novo synthesized gene libraries

Also Published As

Publication number Publication date
CA2320714A1 (en) 2000-07-20
CA2320697A1 (en) 2000-07-20
EP1062614A1 (en) 2000-12-27
IL138002A0 (en) 2001-10-31
AU3210200A (en) 2000-08-01
AU3210100A (en) 2000-08-01
DK1072010T3 (en) 2010-06-21
IL137868A0 (en) 2001-10-31
WO2000042561A2 (en) 2000-07-20
EP1108781A3 (en) 2001-09-12
US7058515B1 (en) 2006-06-06
JP2002534966A (en) 2002-10-22
WO2000042560A2 (en) 2000-07-20
EP1108783A2 (en) 2001-06-20
EP1072010A2 (en) 2001-01-31
EP2253704B1 (en) 2015-08-19
ES2341217T3 (en) 2010-06-17
EP1108781A2 (en) 2001-06-20
JP2002534965A (en) 2002-10-22
EP1108783A3 (en) 2001-09-05
EP2253704A1 (en) 2010-11-24
WO2000042561A3 (en) 2000-12-07
CA2320697C (en) 2014-11-18
ATE465459T1 (en) 2010-05-15
DE60044223D1 (en) 2010-06-02
EP1072010B1 (en) 2010-04-21
EP1130093A1 (en) 2001-09-05

Similar Documents

Publication Publication Date Title
WO2000042560A3 (en) Methods for making character strings, polynucleotides and polypeptides
DK448988A (en) PREPARATION OF AN ALFA AMIDIZATION ENZYME AND METHOD OF PREPARING AND USING THEREOF
ATE216427T1 (en) METHOD FOR PRODUCING POLYPEPTIDE DERIVATIVES
ATE309342T1 (en) METHOD FOR PRODUCING RECOMBINED NUCLEOTIDE SEQUENCES IN VITRO, AND GENE BANKS AND SEQUENCES PRODUCED THEREFORE
WO2001045492A3 (en) Protein kinase stress-related proteins and methods of use in plants
DE60213826T8 (en) DEVELOPMENT OF NEW MOLECULAR FUNCTIONS
DE69940984D1 (en) Method for mitigating pest infestation of plants
EP1652928A4 (en) Plant producing hyaluronic acid
ATE470718T1 (en) RECOMBINANT VIRAL SWITCHES FOR CONTROLLING GENE EXPRESSION IN PLANTS
ATE320486T1 (en) ISOLATION OF BIOLOGICAL MODULATORS FROM LIBRARIES WITH BIOLOGICALLY DIVERSE GENE FRAGMENTS
NO961165L (en) Method and kit for efficient sequencing of nucleic acid
ATE283917T1 (en) AGOUTI RELATED GEN
DE60323067D1 (en) METHOD FOR GENERATING DOUBLE-STRENGTHED DNA WITH A 3'-SINGLE STRING PART AND USE THESE COMPLEXES FOR RECOMBINATION
DE69728878D1 (en) METHOD FOR PROPAGING HEMOPROTE PRODUCTION IN FILAMENTING FUNGI
Stein et al. A model for the origin of biological catalysis.
WO2001075132A3 (en) Method for producing authentic cytokines in plants
EP0763599A3 (en) DNA encoding thermostable pyrophosphatase
WO2004057003A3 (en) Method for producing aminoacids
WO2002088338A3 (en) Method for replicating the hepatitis c virus
DE60234377D1 (en) STRESS ASSOCIATED, PROTEIN KINASE-SPECIFIC POLYPEPTIDES AND METHOD FOR USE IN PLANTS
WO2004113539A3 (en) Method for producing a recombinant protein of interest and protein thus produced
WO2003057899A3 (en) Ion transporter stress-related polypeptides and methods of use in plants
WO2002068634A3 (en) Method for producing gene libraries
ATE319821T1 (en) METHOD AND PRODUCT FOR REGULATING THE RESPONSE OF CELLS TO EXTERNAL SIGNALS

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

ENP Entry into the national phase

Ref document number: 2320714

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 138002

Country of ref document: IL

WWE Wipo information: entry into national phase

Ref document number: 32101/00

Country of ref document: AU

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: PA/a/2000/009026

Country of ref document: MX

WWE Wipo information: entry into national phase

Ref document number: 1020007010375

Country of ref document: KR

WPC Withdrawal of priority claims after completion of the technical preparations for international publication
WWE Wipo information: entry into national phase

Ref document number: 2000909922

Country of ref document: EP

AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWP Wipo information: published in national office

Ref document number: 2000909922

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: 1020007010375

Country of ref document: KR

WWW Wipo information: withdrawn in national office

Ref document number: 2000909922

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1020007010375

Country of ref document: KR