WO1998043042A1 - Spectral bio-imaging methods for cell classification - Google Patents
Spectral bio-imaging methods for cell classification Download PDFInfo
- Publication number
- WO1998043042A1 WO1998043042A1 PCT/US1998/004867 US9804867W WO9843042A1 WO 1998043042 A1 WO1998043042 A1 WO 1998043042A1 US 9804867 W US9804867 W US 9804867W WO 9843042 A1 WO9843042 A1 WO 9843042A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- classification
- spectral
- pixels
- cell
- image
- Prior art date
Links
- 230000003595 spectral effect Effects 0.000 title claims abstract description 190
- 238000003384 imaging method Methods 0.000 title claims abstract description 60
- 238000001228 spectrum Methods 0.000 claims abstract description 145
- 238000000034 method Methods 0.000 claims abstract description 124
- 230000003287 optical effect Effects 0.000 claims abstract description 59
- 210000004027 cell Anatomy 0.000 claims description 182
- 206010028980 Neoplasm Diseases 0.000 claims description 60
- 238000004422 calculation algorithm Methods 0.000 claims description 50
- 238000013528 artificial neural network Methods 0.000 claims description 44
- 238000005259 measurement Methods 0.000 claims description 27
- 201000011510 cancer Diseases 0.000 claims description 26
- 210000004940 nucleus Anatomy 0.000 claims description 18
- 108010077544 Chromatin Proteins 0.000 claims description 16
- 210000003483 chromatin Anatomy 0.000 claims description 16
- 238000010186 staining Methods 0.000 claims description 14
- 239000006185 dispersion Substances 0.000 claims description 10
- 230000001427 coherent effect Effects 0.000 claims description 9
- 201000009030 Carcinoma Diseases 0.000 claims description 5
- 206010025323 Lymphomas Diseases 0.000 claims description 5
- 206010039491 Sarcoma Diseases 0.000 claims description 5
- 210000000805 cytoplasm Anatomy 0.000 claims description 5
- 230000003426 interchromosomal effect Effects 0.000 claims description 5
- 208000032839 leukemia Diseases 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 238000002738 Giemsa staining Methods 0.000 claims description 4
- 238000007490 hematoxylin and eosin (H&E) staining Methods 0.000 claims description 3
- 238000007447 staining method Methods 0.000 claims description 2
- 238000013425 morphometry Methods 0.000 abstract description 20
- 230000003562 morphometric effect Effects 0.000 abstract description 15
- 239000013598 vector Substances 0.000 description 43
- 239000000523 sample Substances 0.000 description 38
- 230000006870 function Effects 0.000 description 35
- 238000004458 analytical method Methods 0.000 description 29
- 210000002569 neuron Anatomy 0.000 description 25
- 208000026310 Breast neoplasm Diseases 0.000 description 24
- 206010073096 invasive lobular breast carcinoma Diseases 0.000 description 21
- 230000001413 cellular effect Effects 0.000 description 19
- 238000000513 principal component analysis Methods 0.000 description 18
- 206010006187 Breast cancer Diseases 0.000 description 17
- 238000000701 chemical imaging Methods 0.000 description 17
- 238000011065 in-situ storage Methods 0.000 description 17
- 238000012549 training Methods 0.000 description 17
- 238000004611 spectroscopical analysis Methods 0.000 description 15
- 230000005540 biological transmission Effects 0.000 description 14
- 238000013459 approach Methods 0.000 description 13
- 201000003714 breast lobular carcinoma Diseases 0.000 description 12
- 208000035269 cancer or benign tumor Diseases 0.000 description 12
- 230000000875 corresponding effect Effects 0.000 description 12
- 239000011159 matrix material Substances 0.000 description 12
- 230000008520 organization Effects 0.000 description 12
- 208000000265 Lobular Carcinoma Diseases 0.000 description 11
- 239000000470 constituent Substances 0.000 description 11
- 238000003745 diagnosis Methods 0.000 description 11
- 230000035945 sensitivity Effects 0.000 description 11
- 239000007787 solid Substances 0.000 description 11
- 210000001519 tissue Anatomy 0.000 description 11
- 208000006402 Ductal Carcinoma Diseases 0.000 description 10
- 206010000496 acne Diseases 0.000 description 10
- 230000008901 benefit Effects 0.000 description 10
- 238000011156 evaluation Methods 0.000 description 10
- 238000000386 microscopy Methods 0.000 description 10
- 238000004393 prognosis Methods 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
- 238000007619 statistical method Methods 0.000 description 9
- 230000007170 pathology Effects 0.000 description 8
- 238000012545 processing Methods 0.000 description 8
- 230000005855 radiation Effects 0.000 description 8
- 230000002596 correlated effect Effects 0.000 description 7
- 239000000975 dye Substances 0.000 description 7
- 238000011282 treatment Methods 0.000 description 7
- 108020004414 DNA Proteins 0.000 description 6
- 238000004364 calculation method Methods 0.000 description 6
- 239000003086 colorant Substances 0.000 description 6
- 230000008878 coupling Effects 0.000 description 6
- 238000010168 coupling process Methods 0.000 description 6
- 238000005859 coupling reaction Methods 0.000 description 6
- 238000009826 distribution Methods 0.000 description 6
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 6
- 238000010191 image analysis Methods 0.000 description 6
- 238000013507 mapping Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 230000009466 transformation Effects 0.000 description 6
- KFZNPGQYVZZSNV-UHFFFAOYSA-M azure B Chemical compound [Cl-].C1=CC(N(C)C)=CC2=[S+]C3=CC(NC)=CC=C3N=C21 KFZNPGQYVZZSNV-UHFFFAOYSA-M 0.000 description 5
- 210000000481 breast Anatomy 0.000 description 5
- 201000008275 breast carcinoma Diseases 0.000 description 5
- 230000036541 health Effects 0.000 description 5
- 206010073095 invasive ductal breast carcinoma Diseases 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 230000000877 morphologic effect Effects 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 5
- 238000012935 Averaging Methods 0.000 description 4
- 208000037162 Ductal Breast Carcinoma Diseases 0.000 description 4
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 4
- 230000004069 differentiation Effects 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 201000004933 in situ carcinoma Diseases 0.000 description 4
- 230000004060 metabolic process Effects 0.000 description 4
- 230000000394 mitotic effect Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000010183 spectrum analysis Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000002560 therapeutic procedure Methods 0.000 description 4
- 238000002834 transmittance Methods 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 210000002308 embryonic cell Anatomy 0.000 description 3
- 238000000799 fluorescence microscopy Methods 0.000 description 3
- 238000002189 fluorescence spectrum Methods 0.000 description 3
- 230000003118 histopathologic effect Effects 0.000 description 3
- 230000010354 integration Effects 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 230000003902 lesion Effects 0.000 description 3
- 230000007774 longterm Effects 0.000 description 3
- 229920002521 macromolecule Polymers 0.000 description 3
- 208000016044 mixed lobular and ductal breast carcinoma Diseases 0.000 description 3
- 238000007491 morphometric analysis Methods 0.000 description 3
- 239000003973 paint Substances 0.000 description 3
- 230000010287 polarization Effects 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 206010027476 Metastases Diseases 0.000 description 2
- 125000000129 anionic group Chemical group 0.000 description 2
- 230000002146 bilateral effect Effects 0.000 description 2
- 235000009120 camo Nutrition 0.000 description 2
- 244000213578 camo Species 0.000 description 2
- 231100000504 carcinogenesis Toxicity 0.000 description 2
- 125000002091 cationic group Chemical group 0.000 description 2
- 210000003855 cell nucleus Anatomy 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 230000010428 chromatin condensation Effects 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 238000010224 classification analysis Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000002380 cytological effect Effects 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 208000028715 ductal breast carcinoma in situ Diseases 0.000 description 2
- 238000000684 flow cytometry Methods 0.000 description 2
- HLUCICHZHWJHLL-UHFFFAOYSA-N hematein Chemical compound C12=CC=C(O)C(O)=C2OCC2(O)C1=C1C=C(O)C(=O)C=C1C2 HLUCICHZHWJHLL-UHFFFAOYSA-N 0.000 description 2
- 230000002962 histologic effect Effects 0.000 description 2
- 238000012333 histopathological diagnosis Methods 0.000 description 2
- 208000024312 invasive carcinoma Diseases 0.000 description 2
- 238000012886 linear function Methods 0.000 description 2
- 239000004973 liquid crystal related substance Substances 0.000 description 2
- 230000009401 metastasis Effects 0.000 description 2
- 238000010606 normalization Methods 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 210000003463 organelle Anatomy 0.000 description 2
- 210000004205 output neuron Anatomy 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000005316 response function Methods 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 238000012800 visualization Methods 0.000 description 2
- RCRNXDJXXKPOTE-LURJTMIESA-N (2s)-2-amino-4-tert-butylsulfanylbutanoic acid Chemical compound CC(C)(C)SCC[C@H](N)C(O)=O RCRNXDJXXKPOTE-LURJTMIESA-N 0.000 description 1
- AGIJRRREJXSQJR-UHFFFAOYSA-N 2h-thiazine Chemical compound N1SC=CC=C1 AGIJRRREJXSQJR-UHFFFAOYSA-N 0.000 description 1
- CGBMFNIHABGKSQ-UHFFFAOYSA-N 9H-xanthen-1-ol hydrobromide Chemical compound Br.O1C2=CC=CC=C2CC2=C1C=CC=C2O CGBMFNIHABGKSQ-UHFFFAOYSA-N 0.000 description 1
- 241001270131 Agaricus moelleri Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- KSFOVUSSGSKXFI-GAQDCDSVSA-N CC1=C/2NC(\C=C3/N=C(/C=C4\N\C(=C/C5=N/C(=C\2)/C(C=C)=C5C)C(C=C)=C4C)C(C)=C3CCC(O)=O)=C1CCC(O)=O Chemical compound CC1=C/2NC(\C=C3/N=C(/C=C4\N\C(=C/C5=N/C(=C\2)/C(C=C)=C5C)C(C=C)=C4C)C(C)=C3CCC(O)=O)=C1CCC(O)=O KSFOVUSSGSKXFI-GAQDCDSVSA-N 0.000 description 1
- 101000822695 Clostridium perfringens (strain 13 / Type A) Small, acid-soluble spore protein C1 Proteins 0.000 description 1
- 101000655262 Clostridium perfringens (strain 13 / Type A) Small, acid-soluble spore protein C2 Proteins 0.000 description 1
- 108050006400 Cyclin Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 108010022894 Euchromatin Proteins 0.000 description 1
- 108010034791 Heterochromatin Proteins 0.000 description 1
- 206010050808 Hyperchromasia Diseases 0.000 description 1
- 208000037396 Intraductal Noninfiltrating Carcinoma Diseases 0.000 description 1
- 241000428199 Mustelinae Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 101000655256 Paraclostridium bifermentans Small, acid-soluble spore protein alpha Proteins 0.000 description 1
- 101000655264 Paraclostridium bifermentans Small, acid-soluble spore protein beta Proteins 0.000 description 1
- 102000009339 Proliferating Cell Nuclear Antigen Human genes 0.000 description 1
- 244000007853 Sarothamnus scoparius Species 0.000 description 1
- 108010085012 Steroid Receptors Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000006096 absorbing agent Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 208000014581 breast ductal adenocarcinoma Diseases 0.000 description 1
- 201000010983 breast ductal carcinoma Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000002059 diagnostic imaging Methods 0.000 description 1
- 238000003748 differential diagnosis Methods 0.000 description 1
- 238000011496 digital image analysis Methods 0.000 description 1
- KSCFJBIXMNOVSH-UHFFFAOYSA-N dyphylline Chemical compound O=C1N(C)C(=O)N(C)C2=C1N(CC(O)CO)C=N2 KSCFJBIXMNOVSH-UHFFFAOYSA-N 0.000 description 1
- 238000000295 emission spectrum Methods 0.000 description 1
- SEACYXSIPDVVMV-UHFFFAOYSA-L eosin Y Chemical compound [Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C([O-])=C(Br)C=C21 SEACYXSIPDVVMV-UHFFFAOYSA-L 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 102000015694 estrogen receptors Human genes 0.000 description 1
- 108010038795 estrogen receptors Proteins 0.000 description 1
- 210000000632 euchromatin Anatomy 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 201000007741 female breast cancer Diseases 0.000 description 1
- 201000002276 female breast carcinoma Diseases 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000002509 fluorescent in situ hybridization Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 210000004458 heterochromatin Anatomy 0.000 description 1
- 238000010562 histological examination Methods 0.000 description 1
- 210000003917 human chromosome Anatomy 0.000 description 1
- 238000003703 image analysis method Methods 0.000 description 1
- 238000003709 image segmentation Methods 0.000 description 1
- 238000012744 immunostaining Methods 0.000 description 1
- 210000002364 input neuron Anatomy 0.000 description 1
- 238000005305 interferometry Methods 0.000 description 1
- 230000016507 interphase Effects 0.000 description 1
- 208000030776 invasive breast carcinoma Diseases 0.000 description 1
- 208000023418 invasive ductal and lobular carcinoma Diseases 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 208000028138 melanocytic neoplasm Diseases 0.000 description 1
- 201000001441 melanoma Diseases 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 235000020938 metabolic status Nutrition 0.000 description 1
- 238000002409 microspectrofluorometry Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 238000005535 overpotential deposition Methods 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 238000003909 pattern recognition Methods 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 150000004713 phosphodiesters Chemical group 0.000 description 1
- 230000000886 photobiology Effects 0.000 description 1
- 238000001907 polarising light microscopy Methods 0.000 description 1
- 229950003776 protoporphyrin Drugs 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000002207 retinal effect Effects 0.000 description 1
- -1 stained dark blue Proteins 0.000 description 1
- 238000011272 standard treatment Methods 0.000 description 1
- 102000005969 steroid hormone receptors Human genes 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 150000004897 thiazines Chemical class 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 238000000411 transmission spectrum Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/12—Generating the spectrum; Monochromators
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/12—Generating the spectrum; Monochromators
- G01J3/1256—Generating the spectrum; Monochromators using acousto-optic tunable filter
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/12—Generating the spectrum; Monochromators
- G01J3/26—Generating the spectrum; Monochromators using multiple reflection, e.g. Fabry-Perot interferometer, variable interference filters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/2823—Imaging spectrometer
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
- G01J3/4406—Fluorescence spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/45—Interferometric spectrometry
- G01J3/453—Interferometric spectrometry by correlation of the amplitudes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J5/00—Radiation pyrometry, e.g. infrared or optical thermometry
- G01J5/10—Radiation pyrometry, e.g. infrared or optical thermometry using electric radiation detectors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
- G06V10/00—Arrangements for image or video recognition or understanding
- G06V10/88—Image or video recognition using optical means, e.g. reference filters, holographic masks, frequency domain filters or spatial domain filters
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
- G06V20/00—Scenes; Scene-specific elements
- G06V20/60—Type of objects
- G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J2003/2866—Markers; Calibrating of scan
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
Landscapes
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Urology & Nephrology (AREA)
- Pathology (AREA)
- Genetics & Genomics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Multimedia (AREA)
- Theoretical Computer Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP54574798A JP2001523334A (en) | 1997-03-25 | 1998-03-16 | Spectral bioimaging method for cell classification |
IL13195398A IL131953A (en) | 1997-03-25 | 1998-03-16 | Spectral bio-imaging methods for cell classifications |
EP98910346A EP1015844A4 (en) | 1997-03-25 | 1998-03-16 | Spectral bio-imaging methods for cell classification |
AU64607/98A AU6460798A (en) | 1997-03-25 | 1998-03-16 | Spectral bio-imaging methods for cell classification |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/824,234 | 1997-03-25 | ||
US08/824,234 US5991028A (en) | 1991-02-22 | 1997-03-25 | Spectral bio-imaging methods for cell classification |
Publications (2)
Publication Number | Publication Date |
---|---|
WO1998043042A1 true WO1998043042A1 (en) | 1998-10-01 |
WO1998043042A9 WO1998043042A9 (en) | 1999-03-04 |
Family
ID=25240913
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1998/004867 WO1998043042A1 (en) | 1997-03-25 | 1998-03-16 | Spectral bio-imaging methods for cell classification |
Country Status (6)
Country | Link |
---|---|
US (1) | US5991028A (en) |
EP (1) | EP1015844A4 (en) |
JP (1) | JP2001523334A (en) |
AU (1) | AU6460798A (en) |
IL (1) | IL131953A (en) |
WO (1) | WO1998043042A1 (en) |
Cited By (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003503135A (en) * | 1999-07-02 | 2003-01-28 | ハイパーメッド イメイジング インコーポレイテッド | Imaging device and sample analysis method |
JP2003513268A (en) * | 1999-10-29 | 2003-04-08 | サイティック コーポレイション | Cytological imaging system and method |
WO2003091729A1 (en) * | 2002-04-26 | 2003-11-06 | Azurtec, Inc. | Bio-spectral imaging system and methods for diagnosing cell disease state |
WO2006081547A1 (en) * | 2005-01-27 | 2006-08-03 | Cambridge Research And Instrumentation, Inc. | Classifying image features |
EP2378275A1 (en) | 2006-12-20 | 2011-10-19 | Ventana Medical Systems, Inc. | Quantitative, multispectral image analysis of tissue specimens stained with quantum dots |
US8126205B2 (en) | 2006-09-25 | 2012-02-28 | Cambridge Research & Instrumentation, Inc. | Sample imaging and classification |
US8199999B2 (en) | 2008-06-17 | 2012-06-12 | Cambridge Research & Instrumentation, Inc. | Image classifier training |
US8280134B2 (en) | 2008-09-22 | 2012-10-02 | Cambridge Research & Instrumentation, Inc. | Multi-spectral imaging including at least one common stain |
US8330087B2 (en) | 2007-10-16 | 2012-12-11 | Cambridge Research & Instrumentation, Inc. | Spectral imaging system with dynamic optical correction |
US8385615B2 (en) | 2003-09-23 | 2013-02-26 | Cambridge Research & Instrumentation, Inc. | Spectral imaging of biological samples |
US8462981B2 (en) | 2010-04-07 | 2013-06-11 | Cambridge Research & Instrumentation, Inc. | Spectral unmixing for visualization of samples |
US8644580B2 (en) | 2008-08-07 | 2014-02-04 | Cambridge Research & Instrumentation, Inc. | Detection of RNA in tissue samples |
US8649580B2 (en) | 2009-09-01 | 2014-02-11 | Olympus Corporation | Image processing method, image processing apparatus, and computer-readable recording medium storing image processing program |
US8879812B2 (en) | 2003-09-23 | 2014-11-04 | Cambridge Research & Instrumentation, Inc. | Spectral imaging of biological samples |
US9002077B2 (en) | 2009-08-10 | 2015-04-07 | Cambridge Research & Instrumentation, Inc. | Visualization of stained samples |
US9418414B2 (en) | 2012-05-30 | 2016-08-16 | Panasonic Intellectual Property Management Co., Ltd. | Image measurement apparatus, image measurement method and image measurement system |
US9541504B2 (en) | 2010-08-05 | 2017-01-10 | Cambridge Research & Instrumentation, Inc. | Enhancing visual assessment of samples |
CN109073547A (en) * | 2016-05-09 | 2018-12-21 | 住友电气工业株式会社 | Tumour cell detection method and tumour cell detection device |
FR3074596A1 (en) * | 2017-12-01 | 2019-06-07 | Universite De Reims Champagne Ardenne | SAMPLE CHARACTERIZATION METHOD USING NEURON NETWORKS |
US10799111B2 (en) | 2014-06-10 | 2020-10-13 | Carl Zeiss Meditec, Inc. | Frequency-domain interferometric based imaging systems and methods |
Families Citing this family (130)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6718053B1 (en) * | 1996-11-27 | 2004-04-06 | Chromavision Medical Systems, Inc. | Method and apparatus for automated image analysis of biological specimens |
US6215892B1 (en) * | 1995-11-30 | 2001-04-10 | Chromavision Medical Systems, Inc. | Method and apparatus for automated image analysis of biological specimens |
US6738529B1 (en) * | 1996-10-09 | 2004-05-18 | Symyx Technologies, Inc. | Analysis of chemical data from images |
CA2279574C (en) | 1997-01-31 | 2007-07-24 | The Horticulture & Food Research Institute Of New Zealand Ltd. | Optical apparatus |
US6466687B1 (en) * | 1997-02-12 | 2002-10-15 | The University Of Iowa Research Foundation | Method and apparatus for analyzing CT images to determine the presence of pulmonary tissue pathology |
EP0980523B1 (en) * | 1997-05-01 | 2006-12-27 | Sophion Bioscience A/S | An automatic electrode positioning apparatus |
GB9714347D0 (en) * | 1997-07-09 | 1997-09-10 | Oxoid Ltd | Image analysis systems and devices for use therewith |
US6149867A (en) | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
US6400973B1 (en) * | 1998-01-20 | 2002-06-04 | Bowden's Automated Products, Inc. | Arterial blood flow simulator |
US6134002A (en) * | 1999-01-14 | 2000-10-17 | Duke University | Apparatus and method for the rapid spectral resolution of confocal images |
US7151847B2 (en) * | 2001-02-20 | 2006-12-19 | Cytokinetics, Inc. | Image analysis of the golgi complex |
US6651008B1 (en) | 1999-05-14 | 2003-11-18 | Cytokinetics, Inc. | Database system including computer code for predictive cellular bioinformatics |
US6743576B1 (en) | 1999-05-14 | 2004-06-01 | Cytokinetics, Inc. | Database system for predictive cellular bioinformatics |
US6738502B1 (en) | 1999-06-04 | 2004-05-18 | Kairos Scientific, Inc. | Multispectral taxonomic identification |
US6673554B1 (en) * | 1999-06-14 | 2004-01-06 | Trellie Bioinformatics, Inc. | Protein localization assays for toxicity and antidotes thereto |
US6750964B2 (en) * | 1999-08-06 | 2004-06-15 | Cambridge Research And Instrumentation, Inc. | Spectral imaging methods and systems |
AU6530100A (en) * | 1999-08-25 | 2001-03-19 | Resolution Sciences Corporation | System for processing and imaging of samples |
GB2355521A (en) * | 1999-09-30 | 2001-04-25 | Marconi Avionics | An imaging system |
US6665060B1 (en) | 1999-10-29 | 2003-12-16 | Cytyc Corporation | Cytological imaging system and method |
US7208265B1 (en) | 1999-11-24 | 2007-04-24 | Xy, Inc. | Method of cryopreserving selected sperm cells |
US6694048B2 (en) * | 2000-04-20 | 2004-02-17 | Green Vision Systems Ltd. | Method for generating intra-particle morphological concentration/density maps and histograms of a chemically pure particulate substance |
EP1301894B1 (en) * | 2000-04-24 | 2009-06-24 | International Remote Imaging Systems, Inc. | Multi-neural net imaging apparatus and method |
PT1301894E (en) | 2000-04-24 | 2009-08-21 | Iris Int Inc | Multi-neural net imaging apparatus and method |
US7236623B2 (en) * | 2000-04-24 | 2007-06-26 | International Remote Imaging Systems, Inc. | Analyte recognition for urinalysis diagnostic system |
US6944338B2 (en) * | 2000-05-11 | 2005-09-13 | Becton Dickinson And Company | System for identifying clusters in scatter plots using smoothed polygons with optimal boundaries |
WO2001088850A2 (en) * | 2000-05-17 | 2001-11-22 | University Of South Florida | Statistical image analysis |
EP1322788A2 (en) * | 2000-06-23 | 2003-07-02 | Cytokinetics, Inc. | Image analysis for phenotyping sets of mutant cells |
US6738511B1 (en) * | 2000-10-04 | 2004-05-18 | Veeco Instruments, Inc. | Reduced noise sensitivity method and apparatus for converting an interferogram phase map to a surface profile map |
WO2002043486A1 (en) | 2000-11-29 | 2002-06-06 | Xy, Inc. | System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations |
US7713687B2 (en) | 2000-11-29 | 2010-05-11 | Xy, Inc. | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
US7171030B2 (en) * | 2000-11-30 | 2007-01-30 | University Of Medicine & Denistry Of New Jersey | Systems for analyzing microtissue arrays |
US7079673B2 (en) * | 2002-02-05 | 2006-07-18 | University Of Medicine & Denistry Of Nj | Systems for analyzing microtissue arrays |
US7027633B2 (en) * | 2000-11-30 | 2006-04-11 | Foran David J | Collaborative diagnostic systems |
US7218764B2 (en) * | 2000-12-04 | 2007-05-15 | Cytokinetics, Inc. | Ploidy classification method |
FR2817986B1 (en) * | 2000-12-07 | 2003-03-28 | Lyon Ecole Centrale | METHOD FOR CLASSIFYING A COLOR IMAGE ACCORDING TO OUTDOOR OR INDOOR SHOOTING |
US6599694B2 (en) | 2000-12-18 | 2003-07-29 | Cytokinetics, Inc. | Method of characterizing potential therapeutics by determining cell-cell interactions |
EP1368689B1 (en) * | 2001-02-02 | 2006-06-14 | Cellomics, Inc. | Method for estimating the best initial focus position |
US6956961B2 (en) * | 2001-02-20 | 2005-10-18 | Cytokinetics, Inc. | Extracting shape information contained in cell images |
US7016787B2 (en) | 2001-02-20 | 2006-03-21 | Cytokinetics, Inc. | Characterizing biological stimuli by response curves |
WO2002097716A1 (en) * | 2001-05-29 | 2002-12-05 | Tissueinformatics, Inc. | Robust stain detection and quantification for histological specimens based on a physical model for stain absorption |
US6834125B2 (en) * | 2001-06-25 | 2004-12-21 | Science And Technology Corp. | Method of improving a digital image as a function of its dynamic range |
US20040071328A1 (en) * | 2001-09-07 | 2004-04-15 | Vaisberg Eugeni A. | Classifying cells based on information contained in cell images |
US20030087456A1 (en) * | 2001-10-08 | 2003-05-08 | Jones Howland D.T. | Within-sample variance classification of samples |
FR2830962B1 (en) * | 2001-10-12 | 2004-01-30 | Inst Nat Rech Inf Automat | IMAGE PROCESSING DEVICE AND METHOD FOR DETECTION OF EVOLUTIVE LESIONS |
JP4497923B2 (en) * | 2001-12-05 | 2010-07-07 | ザ ジェイ. デビッド グラッドストーン インスティテューツ | Robot microscope inspection system |
MXPA05001100A (en) | 2002-08-01 | 2005-04-28 | Xy Inc | Low pressure sperm cell separation system. |
US8486618B2 (en) | 2002-08-01 | 2013-07-16 | Xy, Llc | Heterogeneous inseminate system |
MXPA05001654A (en) | 2002-08-15 | 2005-10-18 | Xy Inc | High resolution flow cytometer. |
US7169548B2 (en) | 2002-09-13 | 2007-01-30 | Xy, Inc. | Sperm cell processing and preservation systems |
CA2404891C (en) * | 2002-10-25 | 2003-11-18 | Nir Technologies Inc. | Method of in-vivo measurement of fat content of a body and apparatus therefor |
GB0227160D0 (en) * | 2002-11-21 | 2002-12-24 | Qinetiq Ltd | Histological assessment of pleomorphism |
JP2004286666A (en) * | 2003-03-24 | 2004-10-14 | Olympus Corp | Pathological diagnosis support apparatus and pathological diagnosis support program |
MX347048B (en) | 2003-03-28 | 2017-04-07 | Inguran Llc * | Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm. |
US20040197832A1 (en) * | 2003-04-03 | 2004-10-07 | Mor Research Applications Ltd. | Non-invasive prenatal genetic diagnosis using transcervical cells |
US20050181429A1 (en) * | 2003-04-03 | 2005-08-18 | Monaliza Medical Ltd. | Non-invasive prenatal genetic diagnosis using transcervical cells |
ES2541121T3 (en) | 2003-05-15 | 2015-07-16 | Xy, Llc | Efficient classification of haploid cells by flow cytometry systems |
WO2004111832A1 (en) * | 2003-05-27 | 2004-12-23 | Purdue Research Foundation | Improved method for diagnosing and treating breast cancer |
US20050014217A1 (en) * | 2003-07-18 | 2005-01-20 | Cytokinetics, Inc. | Predicting hepatotoxicity using cell based assays |
WO2005010677A2 (en) * | 2003-07-18 | 2005-02-03 | Cytokinetics, Inc. | Characterizing biological stimuli by response curves |
US7235353B2 (en) * | 2003-07-18 | 2007-06-26 | Cytokinetics, Inc. | Predicting hepatotoxicity using cell based assays |
WO2005047515A1 (en) * | 2003-11-14 | 2005-05-26 | The State Of Israel - Ministry Of Agriculture & Rural Development | Transgenic disease resistant banana |
CA2561661C (en) | 2004-03-29 | 2015-11-24 | Monsanto Technology Llc | Sperm suspensions for sorting into x or y chromosome-bearing enriched populations |
US20050273271A1 (en) * | 2004-04-05 | 2005-12-08 | Aibing Rao | Method of characterizing cell shape |
US7323318B2 (en) * | 2004-07-15 | 2008-01-29 | Cytokinetics, Inc. | Assay for distinguishing live and dead cells |
US20070031818A1 (en) * | 2004-07-15 | 2007-02-08 | Cytokinetics, Inc., A Delaware Corporation | Assay for distinguishing live and dead cells |
AR049732A1 (en) | 2004-07-22 | 2006-08-30 | Monsanto Co | PROCESS TO ENRICH A Sperm Cell Population |
JP2006038511A (en) * | 2004-07-23 | 2006-02-09 | Tokyo Univ Of Agriculture & Technology | Soil analyzing method and soil analyzer |
US7490009B2 (en) * | 2004-08-03 | 2009-02-10 | Fei Company | Method and system for spectroscopic data analysis |
US20060050376A1 (en) * | 2004-09-02 | 2006-03-09 | Houston Edward S | Robotic microscopy apparatus for high throughput observation of multicellular organisms |
SE527664C2 (en) * | 2004-09-28 | 2006-05-02 | Bostroem & Holmdahl Biotech Hb | Method and apparatus for reproducibly measuring and quantifying intracellular objects |
JP4214124B2 (en) * | 2005-03-14 | 2009-01-28 | 株式会社バイオエコーネット | Ear thermometer |
US20070135999A1 (en) * | 2005-12-13 | 2007-06-14 | Applied Spectral Imaging Ltd. | Method, apparatus and system for characterizing pathological specimen |
JP4856436B2 (en) * | 2006-02-08 | 2012-01-18 | 日本分光株式会社 | Mapping data display method, program, and apparatus |
GB0608258D0 (en) * | 2006-04-26 | 2006-06-07 | Perkinelmer Singapore Pte Ltd | Spectroscopy using attenuated total internal reflectance (ATR) |
JP4890096B2 (en) * | 2006-05-19 | 2012-03-07 | 浜松ホトニクス株式会社 | Image acquisition apparatus, image acquisition method, and image acquisition program |
JP2008043396A (en) * | 2006-08-11 | 2008-02-28 | Olympus Corp | Endoscope system |
MX2009002397A (en) * | 2006-09-05 | 2009-03-16 | Veridex Llc | Methods for ranking cellular images. |
JP4203831B2 (en) * | 2006-11-30 | 2009-01-07 | 独立行政法人産業技術総合研究所 | Precision measurement method for group refractive index of optical materials |
JP2008148791A (en) * | 2006-12-14 | 2008-07-03 | Olympus Corp | Endoscope system |
US9070006B2 (en) * | 2006-12-19 | 2015-06-30 | Hologic, Inc. | Method and system for processing an image of a biological specimen |
US8280144B2 (en) * | 2007-02-21 | 2012-10-02 | Goldfinch Solutions, Llc | System and method for analyzing material properties using hyperspectral imaging |
US8013991B2 (en) * | 2007-08-08 | 2011-09-06 | Chemimage Corporation | Raman difference spectra based disease classification |
WO2009100404A2 (en) * | 2008-02-06 | 2009-08-13 | Fei Company | A method and system for spectrum data analysis |
JP4985480B2 (en) * | 2008-03-05 | 2012-07-25 | 国立大学法人山口大学 | Method for classifying cancer cells, apparatus for classifying cancer cells, and program for classifying cancer cells |
WO2009146425A1 (en) * | 2008-05-29 | 2009-12-03 | Northeastern University | Method of reconstituting cellular spectra useful for detecting cellular disorders |
WO2010041423A1 (en) * | 2008-10-09 | 2010-04-15 | 日本電気株式会社 | Histopathologic diagnosis support system, histopathologic diagnosis support program, and histopathologic diagnosis support method |
WO2010080611A2 (en) * | 2008-12-19 | 2010-07-15 | The Trustees Of Darthmouth College | Apparatus and method for surgical instrument with integral automated tissue classifier |
US8774469B2 (en) | 2009-01-10 | 2014-07-08 | Goldfinch Solutions, Llc | System and method for analyzing properties of meat using multispectral imaging |
US20100201800A1 (en) * | 2009-02-09 | 2010-08-12 | Olympus Corporation | Microscopy system |
JP2011095225A (en) * | 2009-11-02 | 2011-05-12 | Olympus Corp | Apparatus and method for processing image, and microscope system |
JP5387146B2 (en) * | 2009-06-03 | 2014-01-15 | 日本電気株式会社 | Pathological tissue image analysis apparatus, pathological tissue image analysis method, pathological tissue image analysis program |
JP2011002341A (en) * | 2009-06-18 | 2011-01-06 | Olympus Corp | Microscopic system, specimen observation method, and program |
CA2766219A1 (en) * | 2009-07-24 | 2011-01-27 | Galderma Research & Development | Infrared imaging of cutaneous melanoma |
WO2011097508A2 (en) * | 2010-02-04 | 2011-08-11 | University Of Southern California | Combined spectral and polarimetry imaging and diagnostics |
JP2011185843A (en) | 2010-03-10 | 2011-09-22 | Fujifilm Corp | INTRACELLULAR pH IMAGING METHOD USING FLUORESCENCE LIFETIME AND APPARATUS THEREFOR |
JP5437864B2 (en) | 2010-03-10 | 2014-03-12 | 富士フイルム株式会社 | Operating method of pH measuring device, operating method of detecting device, operating method of biological material analyzing device, and each device |
JP5841315B2 (en) | 2010-04-28 | 2016-01-13 | ソニー株式会社 | Fine particle analyzer |
JP5384453B2 (en) | 2010-09-09 | 2014-01-08 | シャープ株式会社 | Measuring device, measuring system, measuring method, control program, and recording medium |
US8934698B2 (en) * | 2011-06-22 | 2015-01-13 | The Johns Hopkins University | System and device for characterizing cells |
ES2648307T3 (en) | 2011-07-08 | 2017-12-29 | Covestro Deutschland Ag | Procedure for the preparation of diaryl carbonates |
RU2014104305A (en) | 2011-07-08 | 2015-08-20 | Байер Интеллектуэль Проперти Гмбх | METHOD FOR PRODUCING DIARYL CARBONATES |
JP6144916B2 (en) * | 2012-01-30 | 2017-06-07 | キヤノン株式会社 | Biological tissue image noise reduction processing method and apparatus |
US8725237B2 (en) * | 2012-03-19 | 2014-05-13 | Genetic Innovations, Inc. | Devices, systems, and methods for virtual staining |
US8664595B2 (en) | 2012-06-28 | 2014-03-04 | Fei Company | Cluster analysis of unknowns in SEM-EDS dataset |
US9188555B2 (en) | 2012-07-30 | 2015-11-17 | Fei Company | Automated EDS standards calibration |
US9123120B2 (en) * | 2012-08-24 | 2015-09-01 | DR Vision Technologies LLC | Progressive decision for cellular process selection |
JP6124548B2 (en) * | 2012-10-17 | 2017-05-10 | キヤノン株式会社 | Ophthalmic imaging method and ophthalmic apparatus |
US9778215B2 (en) | 2012-10-26 | 2017-10-03 | Fei Company | Automated mineral classification |
US8937282B2 (en) | 2012-10-26 | 2015-01-20 | Fei Company | Mineral identification using mineral definitions including variability |
US9091635B2 (en) | 2012-10-26 | 2015-07-28 | Fei Company | Mineral identification using mineral definitions having compositional ranges |
US9048067B2 (en) | 2012-10-26 | 2015-06-02 | Fei Company | Mineral identification using sequential decomposition into elements from mineral definitions |
US9194800B2 (en) * | 2012-10-29 | 2015-11-24 | Tokitae Llc | Systems, devices, and methods employing angular-resolved scattering and spectrally resolved measurements for classification of objects |
WO2014093838A2 (en) | 2012-12-14 | 2014-06-19 | The J. David Gladstone Institutes | Automated robotic microscopy systems |
US9194829B2 (en) | 2012-12-28 | 2015-11-24 | Fei Company | Process for performing automated mineralogy |
US9786050B2 (en) * | 2013-03-15 | 2017-10-10 | The Board Of Trustees Of The University Of Illinois | Stain-free histopathology by chemical imaging |
WO2015017798A2 (en) * | 2013-08-02 | 2015-02-05 | CRIXlabs, Inc. | Method and system for predicting spatial and temporal distributions of therapeutic substance carriers |
JP2015040818A (en) * | 2013-08-23 | 2015-03-02 | 住友電気工業株式会社 | Method and apparatus for grain classification |
US9714908B2 (en) | 2013-11-06 | 2017-07-25 | Fei Company | Sub-pixel analysis and display of fine grained mineral samples |
US11093869B2 (en) | 2014-02-13 | 2021-08-17 | Brewmetrix Inc. | Analytical system with iterative method of analyzing data in web-based data processor with results display designed for non-experts |
CN104049755B (en) * | 2014-06-18 | 2017-01-18 | 中国科学院自动化研究所 | Information processing method and device |
WO2016080813A1 (en) * | 2014-11-21 | 2016-05-26 | Samsung Electronics Co., Ltd. | Method and apparatus for processing medical image |
US10755078B2 (en) | 2015-04-10 | 2020-08-25 | President And Fellows Of Harvard College | Methods and devices for live cell imaging analysis |
GB2542764A (en) * | 2015-09-23 | 2017-04-05 | Pathxl Ltd | Image processing method and apparatus for normalisation and artefact correction |
EP3190394A3 (en) * | 2015-12-15 | 2017-11-15 | Applied Spectral Imaging Ltd. | System and method for spectral imaging |
JP6944060B2 (en) * | 2017-09-21 | 2021-10-06 | バイタル バイオサイエンセズ インク | Imaging of living tissue or other objects |
US11380422B2 (en) * | 2018-03-26 | 2022-07-05 | Uchicago Argonne, Llc | Identification and assignment of rotational spectra using artificial neural networks |
US20190340589A1 (en) * | 2018-05-02 | 2019-11-07 | SOURCE Ltd. | System and method for optimizing routing of transactions over a computer network |
US20220366709A1 (en) * | 2019-10-10 | 2022-11-17 | Georgia Tech Research Corporation | Label-Free Hematology and Pathology Analysis Using Deep-Ultraviolet Microscopy |
US20220375604A1 (en) * | 2021-04-18 | 2022-11-24 | Mary Hitchcock Memorial Hospital, For Itself And On Behalf Of Dartmouth-Hitchcock Clinic | System and method for automation of surgical pathology processes using artificial intelligence |
CN114136867B (en) * | 2021-11-23 | 2023-10-03 | 清华大学 | Optical path design method and device for fluorescence dispersion of flow cytometer |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5377003A (en) * | 1992-03-06 | 1994-12-27 | The United States Of America As Represented By The Department Of Health And Human Services | Spectroscopic imaging device employing imaging quality spectral filters |
US5539517A (en) * | 1993-07-22 | 1996-07-23 | Numetrix Ltd. | Method for simultaneously measuring the spectral intensity as a function of wavelength of all the pixels of a two dimensional scene |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5134662A (en) * | 1985-11-04 | 1992-07-28 | Cell Analysis Systems, Inc. | Dual color camera microscope and methodology for cell staining and analysis |
US5440388A (en) * | 1993-08-02 | 1995-08-08 | Erickson; Jon W. | Chemical analysis and imaging by discrete fourier transform spectroscopy |
-
1997
- 1997-03-25 US US08/824,234 patent/US5991028A/en not_active Expired - Lifetime
-
1998
- 1998-03-16 WO PCT/US1998/004867 patent/WO1998043042A1/en active Application Filing
- 1998-03-16 IL IL13195398A patent/IL131953A/en not_active IP Right Cessation
- 1998-03-16 EP EP98910346A patent/EP1015844A4/en not_active Withdrawn
- 1998-03-16 AU AU64607/98A patent/AU6460798A/en not_active Abandoned
- 1998-03-16 JP JP54574798A patent/JP2001523334A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5377003A (en) * | 1992-03-06 | 1994-12-27 | The United States Of America As Represented By The Department Of Health And Human Services | Spectroscopic imaging device employing imaging quality spectral filters |
US5539517A (en) * | 1993-07-22 | 1996-07-23 | Numetrix Ltd. | Method for simultaneously measuring the spectral intensity as a function of wavelength of all the pixels of a two dimensional scene |
Non-Patent Citations (1)
Title |
---|
See also references of EP1015844A4 * |
Cited By (43)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4849755B2 (en) * | 1999-07-02 | 2012-01-11 | ハイパースペクトラル・イメージング・インコーポレイテツド | Imaging apparatus and sample analysis method |
JP2003503135A (en) * | 1999-07-02 | 2003-01-28 | ハイパーメッド イメイジング インコーポレイテッド | Imaging device and sample analysis method |
JP2003513268A (en) * | 1999-10-29 | 2003-04-08 | サイティック コーポレイション | Cytological imaging system and method |
WO2003091729A1 (en) * | 2002-04-26 | 2003-11-06 | Azurtec, Inc. | Bio-spectral imaging system and methods for diagnosing cell disease state |
US8385615B2 (en) | 2003-09-23 | 2013-02-26 | Cambridge Research & Instrumentation, Inc. | Spectral imaging of biological samples |
US9588099B2 (en) | 2003-09-23 | 2017-03-07 | Cambridge Research & Intrumentation, Inc. | Spectral imaging |
US8879812B2 (en) | 2003-09-23 | 2014-11-04 | Cambridge Research & Instrumentation, Inc. | Spectral imaging of biological samples |
EP2237189A3 (en) * | 2005-01-27 | 2014-07-16 | Cambridge Research & Instrumentation, Inc. | Classifying image features |
US8639043B2 (en) | 2005-01-27 | 2014-01-28 | Cambridge Research & Instrumentation, Inc. | Classifying image features |
EP3444751A3 (en) * | 2005-01-27 | 2019-02-27 | Cambridge Research & Instrumentation, Inc. | Classifying image features |
US7953264B2 (en) | 2005-01-27 | 2011-05-31 | Cambridge Research & Instrumentation, Inc. | Classifying image features |
US7555155B2 (en) | 2005-01-27 | 2009-06-30 | Cambridge Research & Instrumentation, Inc. | Classifying image features |
WO2006081547A1 (en) * | 2005-01-27 | 2006-08-03 | Cambridge Research And Instrumentation, Inc. | Classifying image features |
US8280140B2 (en) | 2005-01-27 | 2012-10-02 | Cambridge Research & Instrumentation, Inc. | Classifying image features |
US9262697B2 (en) | 2006-09-25 | 2016-02-16 | Cambridge Research & Instrumentation, Inc. | Sample imaging and classification |
US8126205B2 (en) | 2006-09-25 | 2012-02-28 | Cambridge Research & Instrumentation, Inc. | Sample imaging and classification |
US8244021B2 (en) | 2006-12-20 | 2012-08-14 | Ventana Medical Systems, Inc. | Quantitative, multispectral image analysis of tissue specimens stained with quantum dots |
US8290236B2 (en) | 2006-12-20 | 2012-10-16 | Ventana Medical Systems, Inc. | Quantitative, multispectral image analysis of tissue specimens stained with quantum dots |
US8290235B2 (en) | 2006-12-20 | 2012-10-16 | Ventana Medical Systems, Inc | Quantitative, multispectral image analysis of tissue specimens stained with quantum dots |
US8285024B2 (en) | 2006-12-20 | 2012-10-09 | Ventana Medical Systems, Inc. | Quantitative, multispectral image analysis of tissue specimens stained with quantum dots |
US8280141B2 (en) | 2006-12-20 | 2012-10-02 | Ventana Medical Systems, Inc. | Quantitative, multispectral image analysis of tissue specimens stained with quantum dots |
EP2381242A1 (en) | 2006-12-20 | 2011-10-26 | Ventana Medical Systems, Inc. | Quantitative, multispectral image analysis of tissue specimens stained with quantum dots |
EP2378276A1 (en) | 2006-12-20 | 2011-10-19 | Ventana Medical Systems, Inc. | Quantitative, multispectral image analysis of tissue specimens stained with quantum dots |
EP2378275A1 (en) | 2006-12-20 | 2011-10-19 | Ventana Medical Systems, Inc. | Quantitative, multispectral image analysis of tissue specimens stained with quantum dots |
US8798394B2 (en) | 2006-12-20 | 2014-08-05 | Ventana Medical Systems, Inc. | Quantitave, multispectral image analysis of tissue specimens stained with quantum dots |
US8330087B2 (en) | 2007-10-16 | 2012-12-11 | Cambridge Research & Instrumentation, Inc. | Spectral imaging system with dynamic optical correction |
US8379962B2 (en) | 2008-06-17 | 2013-02-19 | Cambridge Research & Instrumentation, Inc. | Image classifier training |
US8199999B2 (en) | 2008-06-17 | 2012-06-12 | Cambridge Research & Instrumentation, Inc. | Image classifier training |
US8644580B2 (en) | 2008-08-07 | 2014-02-04 | Cambridge Research & Instrumentation, Inc. | Detection of RNA in tissue samples |
US8280134B2 (en) | 2008-09-22 | 2012-10-02 | Cambridge Research & Instrumentation, Inc. | Multi-spectral imaging including at least one common stain |
US11644395B2 (en) | 2008-09-22 | 2023-05-09 | Cambridge Research & Instrumentation, Inc. | Multi-spectral imaging including at least one common stain |
US9002077B2 (en) | 2009-08-10 | 2015-04-07 | Cambridge Research & Instrumentation, Inc. | Visualization of stained samples |
US8649580B2 (en) | 2009-09-01 | 2014-02-11 | Olympus Corporation | Image processing method, image processing apparatus, and computer-readable recording medium storing image processing program |
US8666113B2 (en) | 2010-04-07 | 2014-03-04 | Cambridge Research & Instrumentation, Inc. | Spectral unmixing for visualization of samples |
US8462981B2 (en) | 2010-04-07 | 2013-06-11 | Cambridge Research & Instrumentation, Inc. | Spectral unmixing for visualization of samples |
US9541504B2 (en) | 2010-08-05 | 2017-01-10 | Cambridge Research & Instrumentation, Inc. | Enhancing visual assessment of samples |
US9418414B2 (en) | 2012-05-30 | 2016-08-16 | Panasonic Intellectual Property Management Co., Ltd. | Image measurement apparatus, image measurement method and image measurement system |
US10799111B2 (en) | 2014-06-10 | 2020-10-13 | Carl Zeiss Meditec, Inc. | Frequency-domain interferometric based imaging systems and methods |
US11890052B2 (en) | 2014-06-10 | 2024-02-06 | Carl Zeiss Meditec, Inc. | Frequency-domain interferometric based imaging systems and methods |
CN109073547A (en) * | 2016-05-09 | 2018-12-21 | 住友电气工业株式会社 | Tumour cell detection method and tumour cell detection device |
WO2019106179A3 (en) * | 2017-12-01 | 2019-07-25 | Universite De Reims Champagne Ardenne | Method for characterizing samples using neural networks |
FR3074596A1 (en) * | 2017-12-01 | 2019-06-07 | Universite De Reims Champagne Ardenne | SAMPLE CHARACTERIZATION METHOD USING NEURON NETWORKS |
US11828652B2 (en) | 2017-12-01 | 2023-11-28 | Universite De Reims Champagne Ardenne | Method for characterising samples using neural networks |
Also Published As
Publication number | Publication date |
---|---|
EP1015844A1 (en) | 2000-07-05 |
US5991028A (en) | 1999-11-23 |
JP2001523334A (en) | 2001-11-20 |
AU6460798A (en) | 1998-10-20 |
EP1015844A4 (en) | 2005-04-27 |
IL131953A0 (en) | 2001-03-19 |
IL131953A (en) | 2002-08-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US5991028A (en) | Spectral bio-imaging methods for cell classification | |
US5995645A (en) | Method of cancer cell detection | |
WO1998043042A9 (en) | Spectral bio-imaging methods for cell classification | |
US6690817B1 (en) | Spectral bio-imaging data for cell classification using internal reference | |
EP0832417B1 (en) | Method for simultaneous detection of multiple fluorophores for in situ hybridization and chromosome painting | |
Pelagotti et al. | Multispectral imaging of paintings | |
Malik et al. | Fourier transform multipixel spectroscopy for quantitative cytology | |
US7990533B2 (en) | System and method for analyzing biological samples using Raman molecular imaging | |
US8013991B2 (en) | Raman difference spectra based disease classification | |
US7808633B2 (en) | Spectroscopic system and method for predicting outcome of disease | |
US7956996B2 (en) | Distinguishing between invasive ductal carcinoma and invasive lobular carcinoma using raman molecular imaging | |
EP0896631A1 (en) | METHOD FOR SIMULTANEOUS DETECTION OF MULTIPLE FLUOROPHORES FOR $i(IN SITU) HYBRIDIZATION | |
Levenson et al. | Spectral imaging in biomedicine: A selective overview | |
Barshack et al. | Spectral morphometric characterization of breast carcinoma cells | |
Malik et al. | Novel spectral imaging system combining spectroscopy with imaging applications for biology | |
Vo-Dinh | Biological Imaging Spectroscopy | |
Rothmann et al. | Spectral imaging of histological and cytological specimens |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
WWE | Wipo information: entry into national phase |
Ref document number: 131953 Country of ref document: IL |
|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH GM GW HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN ML MR NE SN TD TG |
|
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
COP | Corrected version of pamphlet |
Free format text: PAGES 4/13 AND 13/13, DRAWINGS, REPLACED BY NEW PAGES 4/13 AND 13/13; DUE TO LATE TRANSMITTAL BY THE RECEIVING OFFICE |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
ENP | Entry into the national phase |
Ref country code: JP Ref document number: 1998 545747 Kind code of ref document: A Format of ref document f/p: F |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1998910346 Country of ref document: EP |
|
REG | Reference to national code |
Ref country code: DE Ref legal event code: 8642 |
|
WWP | Wipo information: published in national office |
Ref document number: 1998910346 Country of ref document: EP |
|
NENP | Non-entry into the national phase |
Ref country code: CA |