WO1994026883A3 - Process for dust-free enzyme manufacture - Google Patents

Process for dust-free enzyme manufacture Download PDF

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Publication number
WO1994026883A3
WO1994026883A3 PCT/EP1994/001642 EP9401642W WO9426883A3 WO 1994026883 A3 WO1994026883 A3 WO 1994026883A3 EP 9401642 W EP9401642 W EP 9401642W WO 9426883 A3 WO9426883 A3 WO 9426883A3
Authority
WO
WIPO (PCT)
Prior art keywords
granules
enzyme
dust
pore size
free enzyme
Prior art date
Application number
PCT/EP1994/001642
Other languages
French (fr)
Other versions
WO1994026883A2 (en
WO1994026883A9 (en
Inventor
Carl Sidonius Maria Andela
Jan Feijen
Marc Dillissen
Original Assignee
Gist Brocades Nv
Carl Sidonius Maria Andela
Jan Feijen
Marc Dillissen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades Nv, Carl Sidonius Maria Andela, Jan Feijen, Marc Dillissen filed Critical Gist Brocades Nv
Priority to KR1019950700195A priority Critical patent/KR950702625A/en
Priority to JP6525000A priority patent/JPH07509141A/en
Priority to PL94307854A priority patent/PL307854A1/en
Priority to EP94918349A priority patent/EP0675952A1/en
Priority to AU69705/94A priority patent/AU677342B2/en
Publication of WO1994026883A2 publication Critical patent/WO1994026883A2/en
Publication of WO1994026883A9 publication Critical patent/WO1994026883A9/en
Priority to FI950183A priority patent/FI950183A/en
Publication of WO1994026883A3 publication Critical patent/WO1994026883A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38672—Granulated or coated enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/98—Preparation of granular or free-flowing enzyme compositions

Abstract

New enzyme granules are provided with improved properties. The granules are based on core particles having a good pore size and pore size distribution to allow an enzyme solution to enter into the particle. Accordingly, the core material comprises the enzyme in liquid form, thus eliminating the drawback of processing powdered enzymes. Still, good flowability and metering properties are obtained. The enzyme-containing granules are preferably coated with a film-forming macromolecular material. These granules have lower dustiness and improved mechanical strength. Also a method of preparing these enzyme granules is provided.
PCT/EP1994/001642 1993-05-18 1994-05-18 Process for dust-free enzyme manufacture WO1994026883A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
KR1019950700195A KR950702625A (en) 1993-05-18 1994-05-18 PROCESS FOR DUST-FREE ENZYME MANUFACTURE
JP6525000A JPH07509141A (en) 1993-05-18 1994-05-18 Method for producing enzymes that do not contain fine dust
PL94307854A PL307854A1 (en) 1993-05-18 1994-05-18 Method of obtaining dust-free enzyme
EP94918349A EP0675952A1 (en) 1993-05-18 1994-05-18 Process for dust-free enzyme manufacture
AU69705/94A AU677342B2 (en) 1993-05-18 1994-05-18 Process for dust-free enzyme manufacture
FI950183A FI950183A (en) 1993-05-18 1995-01-16 A method for producing a non-dusting enzyme

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP93201428 1993-05-18
NL93201428.5 1993-05-18

Publications (3)

Publication Number Publication Date
WO1994026883A2 WO1994026883A2 (en) 1994-11-24
WO1994026883A9 WO1994026883A9 (en) 1995-01-05
WO1994026883A3 true WO1994026883A3 (en) 1995-03-02

Family

ID=8213835

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1994/001642 WO1994026883A2 (en) 1993-05-18 1994-05-18 Process for dust-free enzyme manufacture

Country Status (8)

Country Link
EP (1) EP0675952A1 (en)
KR (1) KR950702625A (en)
CN (1) CN1110059A (en)
AU (1) AU677342B2 (en)
CA (1) CA2140530A1 (en)
FI (1) FI950183A (en)
PL (1) PL307854A1 (en)
WO (1) WO1994026883A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE43135E1 (en) 2001-05-18 2012-01-24 Danisco A/S Method of improving dough and bread quality
USRE43341E1 (en) 1995-06-07 2012-05-01 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US8889371B2 (en) 2004-07-16 2014-11-18 Dupont Nutrition Biosciences Aps Lipolytic enzyme: uses thereof in the food industry

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3670284B2 (en) * 1994-02-21 2005-07-13 ノボザイムス アクティーゼルスカブ Method for producing immobilized enzyme preparation and use of immobilized enzyme preparation
US5851975A (en) * 1995-05-29 1998-12-22 Kao Corporation Enzyme-containing granulated substance and preparation process thereof
AU2568797A (en) * 1996-04-12 1997-11-07 Novo Nordisk A/S Enzyme-containing granules and process for the production thereof
TW409035B (en) 1997-06-04 2000-10-21 Gist Brocades Bv Starch-based enzyme granulates
EP0913468A3 (en) * 1997-07-22 2001-06-13 Dsm N.V. Bread improving comüposition
CA2243769A1 (en) * 1997-07-22 1999-01-22 Gist-Brocades B.V. Novel bread improving composition
US6156548A (en) 1997-12-23 2000-12-05 Novo Nordisk A/S Immobilization of enzymes with a fluidized bed for use in an organic medium
JP4307713B2 (en) * 1997-12-23 2009-08-05 ノボザイムス アクティーゼルスカブ Enzyme immobilization method
US6630436B1 (en) 1998-10-30 2003-10-07 The Procter & Gamble Company Impact resistant solid component
GB2343191A (en) * 1998-10-30 2000-05-03 Procter & Gamble Low dusting components for detergent compositions
DE60010381T2 (en) 1999-02-10 2004-09-09 Basf Ag FEED GRANULES CONTAINING ENZYMES
DE19929257A1 (en) * 1999-06-25 2000-12-28 Basf Ag Production of polymer-coated granulated animal feed additive, useful in production of pelletized animal feed, involves granulating mixture of carrier and enzyme and coating with suitable organic polymer
RU2346462C2 (en) 2002-01-15 2009-02-20 Басф Акциенгезелльшафт Granulates containing food enzymes
US7501269B2 (en) 2002-01-15 2009-03-10 Basf Aktiengesellschaft Granulates containing feed-enzymes
DE102006053071A1 (en) * 2006-11-10 2008-05-15 Ab Enzymes Gmbh Protein-containing substance with increased temperature stability
US9560877B2 (en) 2012-03-13 2017-02-07 General Mills, Inc. Cereal-based product with improved eating quality fortified with dietary fiber and/or calcium
US20200318037A1 (en) * 2017-10-16 2020-10-08 Novozymes A/S Low dusting granules

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0193829A2 (en) * 1985-03-05 1986-09-10 SOLVAY ENZYMES, INC. (a Delaware corporation) Dust free particulate enzyme formulation
EP0206418A2 (en) * 1985-06-28 1986-12-30 The Procter & Gamble Company Dry bleach and stable enzyme granular composition
EP0430395A1 (en) * 1989-11-24 1991-06-05 Kingston Diagnostics, L.P. Stabilized dispersed enzymes
US5177013A (en) * 1989-07-31 1993-01-05 Ajinomoto Co., Inc. Preparation of an immobilized lipase having a low water content without drying
EP0542351A1 (en) * 1991-11-11 1993-05-19 Akzo Nobel N.V. Process for the preparation of salt granulates

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2446317A1 (en) * 1979-01-12 1980-08-08 Solvay COMPLEX GRANULES CONTAINING ACTIVE PROTEIN SUBSTANCES AND METHODS FOR THEIR MANUFACTURE, USE, AND REGENERATION
AU677166B2 (en) * 1991-10-07 1997-04-17 Genencor International, Inc. Coated enzyme containing granule

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0193829A2 (en) * 1985-03-05 1986-09-10 SOLVAY ENZYMES, INC. (a Delaware corporation) Dust free particulate enzyme formulation
EP0206418A2 (en) * 1985-06-28 1986-12-30 The Procter & Gamble Company Dry bleach and stable enzyme granular composition
US5177013A (en) * 1989-07-31 1993-01-05 Ajinomoto Co., Inc. Preparation of an immobilized lipase having a low water content without drying
EP0430395A1 (en) * 1989-11-24 1991-06-05 Kingston Diagnostics, L.P. Stabilized dispersed enzymes
EP0542351A1 (en) * 1991-11-11 1993-05-19 Akzo Nobel N.V. Process for the preparation of salt granulates

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE43341E1 (en) 1995-06-07 2012-05-01 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
USRE43135E1 (en) 2001-05-18 2012-01-24 Danisco A/S Method of improving dough and bread quality
US8889371B2 (en) 2004-07-16 2014-11-18 Dupont Nutrition Biosciences Aps Lipolytic enzyme: uses thereof in the food industry

Also Published As

Publication number Publication date
AU6970594A (en) 1994-12-12
EP0675952A1 (en) 1995-10-11
AU677342B2 (en) 1997-04-17
WO1994026883A2 (en) 1994-11-24
CA2140530A1 (en) 1994-11-24
FI950183A0 (en) 1995-01-16
CN1110059A (en) 1995-10-11
PL307854A1 (en) 1995-06-26
KR950702625A (en) 1995-07-29
FI950183A (en) 1995-01-16

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