WO1993025912A3 - Automated preparation of nucleic acids - Google Patents

Automated preparation of nucleic acids Download PDF

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Publication number
WO1993025912A3
WO1993025912A3 PCT/GB1993/001222 GB9301222W WO9325912A3 WO 1993025912 A3 WO1993025912 A3 WO 1993025912A3 GB 9301222 W GB9301222 W GB 9301222W WO 9325912 A3 WO9325912 A3 WO 9325912A3
Authority
WO
WIPO (PCT)
Prior art keywords
assay plate
nucleic acids
automated preparation
controlling
preparing
Prior art date
Application number
PCT/GB1993/001222
Other languages
French (fr)
Other versions
WO1993025912A2 (en
Inventor
Trevor Leonard Hawkins
John Edward Sulston
Andrew Robert Watson
Original Assignee
Medical Res Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Res Council filed Critical Medical Res Council
Priority to AU43439/93A priority Critical patent/AU4343993A/en
Publication of WO1993025912A2 publication Critical patent/WO1993025912A2/en
Publication of WO1993025912A3 publication Critical patent/WO1993025912A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/005—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1081—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane
    • G01N35/109—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane with two horizontal degrees of freedom

Abstract

Disclosed is apparatus for preparing a DNA sequence of interest from a complex sample comprising use of magnetic particles in an assay plate, said apparatus comprising: means for moving an assay plate in three-dimensional space; means for supplying liquids to the assay plate; thermal regulation means for controlling the temperature of the assay plate; magnetic separation means for manipulating magnetic particles in the assay plate; and computer control means for controlling and co-ordinating operation of all the above means and an automated method of preparing DNA from a complex sample.
PCT/GB1993/001222 1992-06-09 1993-06-09 Automated preparation of nucleic acids WO1993025912A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU43439/93A AU4343993A (en) 1992-06-09 1993-06-09 Automated preparation of nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB929212164A GB9212164D0 (en) 1992-06-09 1992-06-09 Preparation of nucleic acids
GB9212164.9 1992-06-09

Publications (2)

Publication Number Publication Date
WO1993025912A2 WO1993025912A2 (en) 1993-12-23
WO1993025912A3 true WO1993025912A3 (en) 1994-03-17

Family

ID=10716759

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/GB1993/001223 WO1993025709A1 (en) 1992-06-09 1993-06-09 Preparation of nucleic acids
PCT/GB1993/001222 WO1993025912A2 (en) 1992-06-09 1993-06-09 Automated preparation of nucleic acids

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/GB1993/001223 WO1993025709A1 (en) 1992-06-09 1993-06-09 Preparation of nucleic acids

Country Status (3)

Country Link
AU (2) AU4344093A (en)
GB (1) GB9212164D0 (en)
WO (2) WO1993025709A1 (en)

Cited By (8)

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Publication number Priority date Publication date Assignee Title
US8691969B2 (en) 1994-12-12 2014-04-08 Life Technologies As Isolation of nucleic acid
US8840848B2 (en) 2010-07-23 2014-09-23 Beckman Coulter, Inc. System and method including analytical units
US8973736B2 (en) 2011-11-07 2015-03-10 Beckman Coulter, Inc. Magnetic damping for specimen transport system
US9046506B2 (en) 2011-11-07 2015-06-02 Beckman Coulter, Inc. Specimen container detection
US9446418B2 (en) 2011-11-07 2016-09-20 Beckman Coulter, Inc. Robotic arm
US9482684B2 (en) 2011-11-07 2016-11-01 Beckman Coulter, Inc. Centrifuge system and workflow
US9506943B2 (en) 2011-11-07 2016-11-29 Beckman Coulter, Inc. Aliquotter system and workflow
US9598723B2 (en) * 1998-05-01 2017-03-21 Gen-Probe Incorporated Automated analyzer for performing a nucleic acid-based assay

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DE4409436A1 (en) * 1994-03-19 1995-09-21 Boehringer Mannheim Gmbh Process for processing nucleic acids
DE4421058A1 (en) 1994-06-16 1995-12-21 Boehringer Mannheim Gmbh Process for the magnetic separation of liquid components
US5705628A (en) * 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
WO1996011406A1 (en) * 1994-10-07 1996-04-18 Flinders Technologies Pty. Ltd. Apparatus and method for storage, purification or reaction and processing of a biopolymer
DE19512368A1 (en) * 1995-04-01 1996-10-02 Boehringer Mannheim Gmbh Nucleic acid release and isolation system
US6919175B1 (en) 1995-04-01 2005-07-19 Roche Diagnostics Gmbh System for releasing and isolating nucleic acids
US5683875A (en) * 1995-05-04 1997-11-04 Hewlett-Packard Company Method for detecting a target nucleic acid analyte in a sample
GB9525794D0 (en) * 1995-12-18 1996-02-21 Hale Alan Biotechnological process
US6277332B1 (en) 1995-12-18 2001-08-21 Solid Phase Sciences Corporation Reaction plenum with magnetic separation and/or ultrasonic agitation
US5779985A (en) * 1995-12-18 1998-07-14 Solid Phase Sciences Corporation Reaction plenum
US6984491B2 (en) 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7098320B1 (en) 1996-07-29 2006-08-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6582921B2 (en) 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
ES2287956T3 (en) 1996-07-29 2007-12-16 Nanosphere Inc. NANOPARTICLES THAT HAVE OLIGONUCLEOTIDES UNITED TO THE SAME AND USES OF THE SAME.
US6506564B1 (en) 1996-07-29 2003-01-14 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6750016B2 (en) 1996-07-29 2004-06-15 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6008010A (en) 1996-11-01 1999-12-28 University Of Pittsburgh Method and apparatus for holding cells
FR2760844B1 (en) * 1997-03-17 1999-05-21 Genethon Ii DEVICE AND METHOD FOR AUTOMATIC ANALYSIS OF SAMPLES ON GELS
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
DE19801730A1 (en) * 1998-01-19 1999-07-22 Fraunhofer Ges Forschung Analyzing cell contents, especially nucleic acids, useful for medical diagnostics
US5912129A (en) * 1998-03-05 1999-06-15 Vinayagamoorthy; Thuraiayah Multi-zone polymerase/ligase chain reaction
US8337753B2 (en) 1998-05-01 2012-12-25 Gen-Probe Incorporated Temperature-controlled incubator having a receptacle mixing mechanism
AU2003271382B2 (en) * 1998-05-01 2006-07-20 Gen-Probe Incorporated Automated Diagnostic Analyzer and Method
AU2006230730B2 (en) * 1998-05-01 2009-03-05 Gen-Probe Incorporated Multiple ring assembly for providing specimen to reaction receptacles within an automated analyzer
DE19854003A1 (en) * 1998-11-18 2000-05-25 Jenoptik Jena Gmbh Simultaneous magnetic particle handling in a two-dimensional arrangement
WO2001002554A2 (en) * 1999-07-05 2001-01-11 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Method for high-throughput selection of interacting molecules
DE19943374A1 (en) * 1999-09-10 2001-03-29 Max Planck Gesellschaft Method for binding nucleic acids to a solid phase
DE19954840A1 (en) * 1999-11-09 2001-05-17 Biopsytec Gmbh Device for the preparation of biological samples for DNA analysis
CA2396113C (en) 2000-01-13 2009-04-07 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
JP2004515208A (en) * 2000-03-28 2004-05-27 ナノスフェアー インコーポレイテッド Oligonucleotide-attached nanoparticles and methods of use
ATE324465T1 (en) 2000-07-11 2006-05-15 Univ Northwestern DETECTION METHOD USING INCREASED SILVER TINTING
JP2002022752A (en) * 2000-07-13 2002-01-23 Suzuki Motor Corp Specimen testing device
AU2001281248A1 (en) * 2000-08-11 2002-03-13 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
EP1978110B1 (en) 2000-09-06 2010-05-26 Transnetyx, Inc. Computer-based method and system for screening genomic DNA
EP1356109A2 (en) * 2000-12-08 2003-10-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
DE10065148B4 (en) * 2000-12-22 2004-01-22 Institut für Physikalische Hochtechnologie e.V. Magnetic gripping-holding arrangement
US7186814B2 (en) 2001-11-09 2007-03-06 Nanosphere, Inc. Bioconjugate-nanoparticle probes
AU2003247788B2 (en) 2002-07-02 2007-04-19 Nanosphere Inc. Nanoparticle polyanion conjugates and methods of use thereof in detecting analytes
WO2006015326A2 (en) 2004-07-30 2006-02-09 Agencourt Bioscience Corporation Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers
JPWO2006059386A1 (en) * 2004-12-02 2008-06-05 独立行政法人農業生物資源研究所 Method for isolating double-stranded DNA containing simple repetitive sequence
US7932081B2 (en) 2005-03-10 2011-04-26 Gen-Probe Incorporated Signal measuring system for conducting real-time amplification assays
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
CN103403533B (en) 2011-02-24 2017-02-15 简.探针公司 Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector
EP2776848B1 (en) 2011-11-07 2019-12-25 Beckman Coulter, Inc. System and method for transporting sample containers

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EP0212663A2 (en) * 1985-08-30 1987-03-04 Tosoh Corporation Automated immunoassay analyser
FR2633310A1 (en) * 1988-06-24 1989-12-29 Pasteur Institut Apparatus for the repeated automatic execution of a thermal cycle in several successive stages, especially for the enzymatic amplification of nucleic acid sequences
EP0438883A2 (en) * 1989-12-22 1991-07-31 Beckman Instruments, Inc. Heated cover device
WO1991016675A1 (en) * 1990-04-06 1991-10-31 Applied Biosystems, Inc. Automated molecular biology laboratory
WO1992005443A1 (en) * 1990-09-15 1992-04-02 Medical Research Council Reagent separation
EP0479448A2 (en) * 1990-10-02 1992-04-08 Beckman Instruments, Inc. Magnetic separation device
EP0351988B1 (en) * 1988-07-16 1993-09-01 Quatro Biosystems Limited Bio-fluid assay apparatus

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GB8507706D0 (en) * 1985-03-25 1985-05-01 Genetics Int Inc Magnetic nucleic acid sequences
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EP0212663A2 (en) * 1985-08-30 1987-03-04 Tosoh Corporation Automated immunoassay analyser
FR2633310A1 (en) * 1988-06-24 1989-12-29 Pasteur Institut Apparatus for the repeated automatic execution of a thermal cycle in several successive stages, especially for the enzymatic amplification of nucleic acid sequences
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EP0438883A2 (en) * 1989-12-22 1991-07-31 Beckman Instruments, Inc. Heated cover device
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Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691969B2 (en) 1994-12-12 2014-04-08 Life Technologies As Isolation of nucleic acid
US9598723B2 (en) * 1998-05-01 2017-03-21 Gen-Probe Incorporated Automated analyzer for performing a nucleic acid-based assay
US9046455B2 (en) 2010-07-23 2015-06-02 Beckman Coulter, Inc. System and method including multiple processing lanes executing processing protocols
US9140715B2 (en) 2010-07-23 2015-09-22 Beckman Coulter, Inc. System and method for controlling thermal cycler modules
US8962308B2 (en) 2010-07-23 2015-02-24 Beckman Coulter, Inc. System and method including thermal cycler modules
US8840848B2 (en) 2010-07-23 2014-09-23 Beckman Coulter, Inc. System and method including analytical units
US8996320B2 (en) 2010-07-23 2015-03-31 Beckman Coulter, Inc. System and method including analytical units
US9519000B2 (en) 2010-07-23 2016-12-13 Beckman Coulter, Inc. Reagent cartridge
US8932541B2 (en) 2010-07-23 2015-01-13 Beckman Coulter, Inc. Pipettor including compliant coupling
US8956570B2 (en) 2010-07-23 2015-02-17 Beckman Coulter, Inc. System and method including analytical units
US9274132B2 (en) 2010-07-23 2016-03-01 Beckman Coulter, Inc. Assay cartridge with reaction well
US9285382B2 (en) 2010-07-23 2016-03-15 Beckman Coulter, Inc. Reaction vessel
US9446418B2 (en) 2011-11-07 2016-09-20 Beckman Coulter, Inc. Robotic arm
US9482684B2 (en) 2011-11-07 2016-11-01 Beckman Coulter, Inc. Centrifuge system and workflow
US9506943B2 (en) 2011-11-07 2016-11-29 Beckman Coulter, Inc. Aliquotter system and workflow
US9046506B2 (en) 2011-11-07 2015-06-02 Beckman Coulter, Inc. Specimen container detection
US8973736B2 (en) 2011-11-07 2015-03-10 Beckman Coulter, Inc. Magnetic damping for specimen transport system

Also Published As

Publication number Publication date
AU4344093A (en) 1994-01-04
WO1993025912A2 (en) 1993-12-23
WO1993025709A1 (en) 1993-12-23
AU4343993A (en) 1994-01-04
GB9212164D0 (en) 1992-07-22

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