DESCRIPTION
PRODUCTION OF HUMAN BASIC FGF MUTEIN ' Technical Field The present invention relates to a technique for producing a mutein of a mature human basic fibroblast growth factor (hereinafter also briefly referred to as hbFGF) in which at least one constituent amino acid of hbFGF is replaced by another amino acid which can be used as a healing promoter for wounds. Background Art
Basic fibroblast growth' factor (bFGF) is a basic polypeptide hormone having a molecular weight of about 17,000 and is mainly secreted by a pituitary gland. bFGF was first isolated as a factor exhibiting strong growth promoting action on fibroblasts such as BALB/c3T3 cell [D. Gospodarowicz, Nature 249, 123 (1974)]. It is now known that FGF exhibits growth promoting action on almost all mesoblast-derived cells [D. Gospodarowicz et al., National Cancer Institute Monograph 48, 109 (1978)]. In particular, the angiogenic action of the bFGF, together with its cell growth promoting action, suggests a potential for the application thereof as a therapeutic medicine for traumas, and as a preventive and therapeutic medicine for thrombosis, arteriosclerosis, etc. Genes coding for hbFGFs have been cloned by Abraham et al. [The EMBO Journal 5, 2523-2528 (1986)] and Kurokawa et al. [FEBS Letters 213, 189-194 (1987)], and expressed in
animal cells [FEBS Letters 213, 189-194 (1987)], yeast [The Journal of Biological Chemistry 263, 16471-16478 (1988)] and Escherichia coli [The Journal of Biological Chemistry 263, 16297-16302 (1988)]. However, their production is not always sufficient and the resulting samples- are too unstable to use as medicines. As a result of various studies to solve the unstability, it has been revealed that compounds in which cysteine residues of hbFGF molecules are replaced by serine residues have high stability and exhibit the same biological activity [Biochemical and Biophysical Research Communication 151, 701-708 (1988)].
Further, there has been developed a method for producing a mutein in which at least one constituent amino acid of hbFGF is replaced by another amino acid by genetic engineering techniques (European Patent Publication No. 281,822). . .
When recombinant proteins are produced using E. coli, water-insoluble inclusion bodies are frequently formed to accumulate the proteins. When the desired substances are isolated and purified from such inclusion bodies, the inclusion bodies are usually solubilized by adding protein denaturing agents. As for hbFGF muteins, however, the proteins thus solubilized are inactive and no technique for reactivating them has been established yet. The hbFGF muteins can be used as medicines if the muteins can be accumulated in large amounts and in an active state by using genetic engineering techniques, and further
isolated and purified in high yield. It is therefore an important object to establish a method for producing the muteins at high efficiency.
In general, when gene products are produced in large amounts by using genetic engineering techniques, the selection of host-vector systems and promoters is important and differs depending on each gene. Various studies on efficient expression systems of hbFGF muteins have revealed that an E. coli gene expression system using a T7 promoter [F. W. Studier et al., Journal of Molecular Biology 189,
113-130 (1986)] is excellent for expression of the genes of the hbFGF muteins. The combination of the T7 promoter and hbFGF mutein genes is novel. This T7 promoter is known to be a strong promoter. In some cases, however, the accumulated proteins such as human interleukin-2, prolactin, form inclusion bodies, and most of them are accumulated in an inactive state. The present inventors have found the interleukin-2 case, and Paris, N. et al. have found the prolactin case [Paris, N. et al., Biotechnology and Applied Biochemistry, 12, 436-449 (1990)].
Disclosure of Invention The present inventors conducted intensive investigations to accumulate considerable amounts of the hbFGF mutein in an active state, and have found that addition of isopropylthiogalactopyranosyde in a low concentration is effective.. In addition, the present inventors have further established a method for isolating
and purifying the hbFGF mutein efficiently, thus completing the present invention.
The present invention provides:
(1) a vector containing a nucleotide sequence coding for a mutein in which at least one constituent amino acid of a mature human basic fibroblast growth factor (hbFGF) is replaced by another amino acid, and a T7 promoter upstream therefrom,
(2) a transformant transformed by the vector described in the above item (1),
(3) the transformant described in the above item (2), in which a host is E^ coli having a T7 RNA polymerase gene downstream from a lac promoter,
(4) a method for producing the mutein in which at least one constituent amino acid of the mature hbFGF is replaced by another amino acid, which comprises cultivating the transformant described in the above item (2) in a culture medium,
(5) the method described in the above item (4), in which about 3 to 500 μM of isopropylthiogalactopyranoside
(hereinafter also briefly referred to as IPTG) is added to the culture medium on a logarithmic growth phase of the transformant described in the above item (3), followed by cultivation, and (6) the method described in the above item (5), in which a resultant mutein-containing solution is purified by chromatography using a crosslinked polysaccharide sulfate, a
__
synthetic polymer having a sulfonic acid group as an exchange group and/or a synthetic polymer for gel filtration as a carrier.
According to the present invention, the substitution type muteins of mature hbFGFs can be efficiently produced. The present invention can therefore be advantageously used for industrial production of the muteins.
Brief description of the Drawings Fig. 1 shows a DNA nucleotide sequence of rhbFGF mutein CS23 used in Example 1 and an amino acid sequence of a protein for which the nucleotide sequence codes;
Fig. 2 is a schematic representation showing the construction of plasmid pTB960 obtained in Example 1;
Fig. 3 shows a SDS-PAGE pattern of a purified sample obtained in Example 5 and a marker; and
Figs. 4 to 6 show patterns of high performance liquid chromatography of the purified sample obtained in Example 5. Figs. 7 to 9 are schematic representations showing the construction of plasmids pHP901, pME901 and pCM901 obtained in Example 8.
Detailed Description of the Inventions The mature hbFGF in the present invention is a peptide consisting of 146 amino acids, counting the amino acid Pro next to Met of the N-terminus as the 1st and the amino acid Ser of the C-terminus as the 146th, in Fig. 1. .
Examples of the hbFGF muteins as shown in the present invention include muteins in which at least one constituent
amino acid of the mature hbFGF is replaced by another amino acid, as described in European Patent Publication No. 281,822 and Biochemical and Biophysical Research Communication 151, 701-708 (1988). As to the number of hbFGF-constituent amino acids before substitution in the mutein, which has at least one hbFGF-constituent amino acid substituted by another amino acid, it may be any number as long as FGF characteristics, such as the characteristics of angiogenesis, cell growth stimulating activity and cell differentiating activity, are not lost.
Examples of the constituent amino acids before substitution include cysteine and amino acids other than cysteine. In particular, cysteine is preferred. The amino acids other than cysteine as the constituent amino acids before substitution include aspartic acid, arginine, glycine and valine.
When the constituent amino acid before substitution is cysteine, neutral amino acids are preferred as the substituting amino acids. .Specific examples of the neutral amino acids include glycine, valine, alanine, leucine, isoleucine, tyrosine, phenylalanine, histidine, tryptophan, serine, threonine and methionine. In particular, serine and threonine are preferred. When the constituent amino acid before substitution is an amino acid other than cysteine, there are selected as the other substituting amino acids, for example, amino acids
different in hydrophilicity, hydrophobicity or electric charge from the constituent amino acid before substitution. Specifically, when the amino acid before substitution is aspartic acid, the substituting amino acids include asparagine, threonine, valine, phenylalanine and arginine, Asparagine and arginine are particularly preferred.
When the amino acid before substitution is arginine, the substituting amino acids include glutamine, threonine, leucine, phenylalanine and aspartic acid. Glutamine is especially preferable.
When the constituent amino acid before substitution is glycine, the substituting amino acids include threonine, leucine, phenylalanine, serine, glutamic acid and arginine. Threonine is particularly preferable. When the constituent amino acid before substitution is serine, the substituting amino acids include methionine, alanine, leucine, cysteine, glutamine, arginine and aspartic acid. In particular, methionine is preferred.
When the constituent amino acid before substitution is valine, the substituting amino acids include serine, leucine, proline, glycine, lysine and aspartic acid. Serine is especially preferred.
As the original constituent amino acids before substitution, aspartic acid, arginine, glycine, serine and valine are preferably selected.
As the substituting amino acids, asparagine, glutamine, arginine, threonine, methionine, serine and leucine are
preferably selected.
The most preferred substituted muteins include a mutein in which cysteine, the constituent amino acid, is replaced by serine. In the above substitution, the substitution of at least two constituent amino acids may be simultaneously carried out. In particular, it is preferable to substitute two or three constituent amino acids.
Preferred examples of the hbFGF muteins in the present invention include a mutein in which at least one cysteine residue of the mature hbFGF mutein is replaced by a serine residue.
As the mutein, recombinant hbFGF mutein CS23 (hereinafter also briefly referred to as rhbFGF mutein CS23) is particularly preferred in which cysteine residues at the 69- and 87- positions of the mature hbFGF are replaced by serine residues, respectively. The position of the amino acids of the above hbFGF are numbered, by counting the amino acid Pro next to Met of the N-terminus of the amino acid sequence as shown in Fig. 1 as the 1st.
In order to produce the muteins, site-directed mutagenesis is employed. This technique is well known and described in R. F. Lather and J. P. Lecoq, Genetic Engineering, p.31-50, Academic Press (1983). Mutagenesis directed to oligonucleotide is described in M. Smith and S. Gillam, Genetic Engineering: Principles and Methods, vol.3, p.1-32, Plenum Press (1981).
The production of a structural gene which encodes the mutein is carried out, for example, by the steps of:
(a) hybridizing a single-stranded DNA comprising a single strand of the structural gene of the hbFGF with a mutagenic oligonucleotide primer (the above primer is complementary to a region including a codon for cysteine to be replaced by this single strand or including an anti-sense triplet which forms a pair with this codon in some cases, provided this does not apply to disparity with other codons for the amino acid than the above codon, or with the anti- sense triplet in some cases),
(b) elongating the primer with DNA polymerase to form a mutational heteroduplex, and
(c) replicating this mutational heteroduplex. Then, phage DNA for transferring the mutagenized gene is isolated and introduced into a plasmid.
As the T7 promoter used in the present invention, there may be used any of 17 kinds of promoters discovered on T7 DNA [J. L. Oakley et al., Proc. Natl. Acad. Sci. U.S.A. 74, 4266-4270 (1977); M. D. Rosa, Cell 16," 815-825 (1979); N. Panayotatos et al., Nature 280, 35 (1979); J. J. Dunn et al., J. Mol. Biol. 166, 477-535 (1983)], but a $Z_>10 promoter [A. H. Rosenberg et al., Gene 56, 125-135 (1987)] is preferably used. As a transcription terminator used in the present invention, any terminator may be used as long as it functions in E. coli systems, but a T <p terminater [F. W.
Studier et al., J. Mol. .Biol. 189, 113-130 (1986) is preferably used.
The T7 RNA polymerase genes used in the present invention include T7 gene 1. [F. W. Studier et al. , J. Mol. Biol. 189, 113-130 (1986)].
Examples of vectors from which the vectors used in the present invention are formed include pBR322, pUC8, pUC9, pMB9, pKC7, pACYC177 and pKN410.
The vectors used in .the present invention are constructed by incorporating the T7 promoter and the T7 terminate-" into the above vectors. Such vectors include pET-1, pET-2, ρET-3, pET-4 and pET-5 [A. H. Rosenberg, Gene 56, 125-135 (1987)], but pET-3C (ibid.) is preferably used. As the host for the transformant used in the present invention, any of E^ coli strains into which the T7 RNA polymerase gene (T7 gene 1) [F. W. Studier et al. ,. J. Mol. Biol. 189, 113-130 (1986)-] is incorporated, such as MM294, DH-1, C600 and BL21, may be used. The strains MM294 and BL21 are preferably used in which a λphage including T7 gene 1 is lysogenized. The T7 RNA polymerase gene can also be harbored as a plasmid having different origin from that of expression vector. In this case, as the promoter for T7 gene 1, .there is used the lac promoter whose expression is induced with isopropyl-1-thio-β-D-galactopyranoside (IPTG) . The transformants used in the present invention are obtained by transforming the E_;_ coli. strains into which the above T7 gene 1 (RNA polymerase gene) have been
incorporated, with the plasmids having the gene-transcription terminators for T7 promoter expression, according to methods known in the art such as the methods described in Proc. Natl. Acad. Sci. U.S.A. 69, 2110 (1972) and Gene 17, 107 (1982). In this case, the hosts to be used may be preliminarily transformed with plasmids having the T7 lysozy e gene so that the resulting transformants have two kinds of different plasmids simultaneously.
When the transformants are cultivated, liquid media are particularly suitable as media used for culture.. Carbon sources, nitrogen sources, inorganic compounds and others necessary for growth of the transformants are contained therein. The carbon sources include, for example, glucose, dextrin, soluble starch and sucrose. The nitrogen sources include inorganic or organic materials such as ammonium salts, nitrates, corn steep liquor, peptone, casein," casamino acids, meat extracts, soybean meal and potato extract solution. Examples of the inorganic compounds include calcium chloride, sodium dihydrogenphosphate, and magnesium chloride. Yeast extracts, vitamins, growth promoting factors and the like may be further added thereto. The pH of the media is desirably about 6 to 8. As the medium used for cultivation of the E_^ coli transformants, there is preferred, for example, M9 medium (Miller, Journal of Experiments in Molecular Genetics, 431-433, Cold Spring Harbor Laboratory, New York, 1972) supplemented with glucose and casamino acids. Iron ion
sources may be further added to this medium. The iron ion sources are materials which dissociate into iron ions in solution or materials utilized in iron ion form. Such materials include salts of iron, preferably ferrous or ferric inorganic salts such as ferrous chloride, ferric chloride, ferrous sulfate, ferric sulfate, ferric phosphate and ferric nitrate. The iron ion sources are added in an amount of about 10 -6 to 10-4 M, preferably in an amount of about 5X10-6 to 5X10~5 M. The cultivation is usually carried out at about 15 to 43°C for about 3 to 72 hours, preferably for about 12 to 48 hours, with aeration or agitation if necessary.
For the gene expression, it is preferable to add IPTG in the course of cultivation. Thereby, T7 gene 1 (RNA polymerase gene) ligated downstream from the lac promoter is expressed, and T7 phage RNA polymerase 1 thus produced specifically recognize the T7 promoter. According to the prior art, IPTG is added in an amount of 0.1 to 20 mM, preferably in an amount of 1 to 2 mM for expression of the lac promoter [up to 1 mM of IPTG: L^bner-Olesen et al., Cell, 57, 881-889 (1989); 2 mM of IPTG: Ernst H. et al. , Gene, 68, 345-355 (1988)]; 20 mM of IPTG: Luck, D. N. et al., DNA, _5, 21-28 (1986)]. However, it has been revealed that proteins accumulated in inducing expression by addition of IPTG of this amount form inclusion bodies, which sometimes results in accumulation of inactive proteins. As a result of various studies on methods for accumulating the
proteins in a dissolved state, it has- been discovered that the object is first attained by adding about 3 to 500 yM, preferably about 3 to 300 μM, more preferably 6 to 200 μM, and most preferably about 6 to 80 μM of IPTG in the course of cultivation. When large-scale cultivation such as tank cultivation is carried out, it is preferred that IPTG is added in an amount of about 10 to 500 μM, preferably 10 to 200 μM, more preferably 10 to 100 μM, still more preferably 10 to 80 μM. When small-scale cultivation such as flask cultivation is carried out, it is preferred that IPTG is added in an amount of about 3 to 100 μM, more preferably 6 to 80 μM. IPTG is first added about 1 to 24 hours, preferably about 3 to 12 hours after the initiation of cultivation, and it is preferred that IPTG is added on a logarithmic growth phase. IPTG is hereafter added intermittently or continuously as required. The media containing IPTG are cultivated at a temperature of about 20 to 42°C, preferably about 20 to 30°C.- In lieu of isopropylthiogalactopyranoside, there are used in some cases, for example, propylthiogalactopyranoside, methylthiogalactopyranoside, butylthiogalactopyranoside and cyclohexylthiogalactopyranoside.
The hbFGF muteins in the present invention can be isolated and purified from the cultures obtained above, for example, by the following methods.
When the hbFGF muteins in the.present invention are extracted from the cultivated cells, the cells are collected
after cultivation by methods known in the art, such as centrifugation. Then the collected cells are subjected to disruption by glass beads, French press, ultrasonic treatment, lysozyme treatment and/or freeze-thawing. In particular, the disruption by glass beads is preferable.
Various studies have been made on methods for purifying the. hbFGF muteins according to the present invention from the supernatants obtained above. As a result, high-purity samples have been obtained in very high yield by combining affinity chromatography using a crosslinked polysaccharide sulfate as a carrier, ion exchange chromatography whose carrier is a synthetic polymer having a sulfonic acid group as an exchange group and chromatography using a synthetic polymer for gel filtration as a carrier with one another at least once.
The crosslinked polysaccharide sulfates used in the present invention include crosslinked cellulose sulfates, crosslinked agarose sulfates and crosslinked dextran sulfates. The above cellulose is a polysaccharide composed of glucose linked by β-1,4 bonds, and its molecular weight is preferably about 50,000 to 2,000,000. Specific examples thereof include Avicel (crystalline cellulose, Asahi Chemical Industry, Japan) and Cellulofine (Chisso Corporation, Japan).
The above agarose is a polysaccharide which is the main component of agar, and has the recurring structure of
D-galactosyl-(βl-^4)-3,6-anhydro-L-garactosyl-(αl-3) . Its molecular weight is preferably about 10,000 to 5,000,000. Specific examples thereof include Sepharose 2B, Sepharose 4B and Sepharose 6B (Pharmacia, Sweden). The above dextran is a D-glucose polymer mainly comprising α(1^6) bonds formed, for example, by the action of a microorganism such as Leuconostoc mesenteroides on sucrose. Its average molecular weight is preferably about 1,000 to 40,000,000. The crosslinked polysaccharide sulfates used in the present invention are prepared by treating crosslinked polysaccharides such as the above dextran, agarose and cellulose with known crosslinking agents such as epichlorohydrin and 2,3- dibromopropanol according to methods known in the art.
The crosslinked polysaccharides are commercially available and can be purchased from Pharmacia (Sweden) under the trade names of Sephadex G-10, Sephadex G-15, Sephadex G- 25, Sephadex G-50 and Sephadex G-100 (crosslinked dextran), and under the trade names of Sepharose. CL-2B, Sepharose CL- 4B and Sepharose CL-6B (crosslinked agarose). Also, crosslinked cellulose can be purchased from Chisso Corporation (Japan) under the trade name of Cellulofine (crosslinked cellulose). The desired crosslinked polysaccharide sulfates can be synthesized by allowing known sulfating agents, such as chlorosulfonic acid and sulfuric anhydride esters, to react with these crosslinked polysaccharides.
Examples of the crosslinked cellulose sulfates include the product put on the market by Seikagaku Kogyo (Japan) under the trade name of Sulfated Cellulofine (crosslinked cellulose sulfate). Examples of the crosslinked dextran sulfates include sulfated Sephadex.
Examples of the crosslinked agarose sulfates include sulfated Sepharose.
The crosslinked polysaccharide sulfates used in the present invention may be in the form of the corresponding salts. ' Examples of the salts include sodium, potassium, ammonium "and trimethylammonium salts. In particular, the sodium salts are preferably used.
The crosslinked polysaccharide sulfates used in the present invention are insoluble in water, and therefore it is preferred to use them in their gelatinous state by hydration.
The methods for purifying the hbFGF muteins using the crosslinked polysaccharide sulfates in the present invention include affinity chromatography described below. hbFGF mutein-containing aqueous media are solutions containing the hbFGF muteins. The aqueous media, include water and media mainly composed of water, and are preferably adjusted to the pH range of about 3 to 10 with buffer solutions such as phosphate buffer, citrate buffer and
Tris-hydrochloric acid buffer, to prevent inactivation of the hbFGF muteins.
The hbFGF mutein-containing solutions are next readjusted to a pH range of about 5.0 to 9.0, and then diluted with distilled water as is required, so that they have an electric conductivity of about 15 mU" or less. The hbFGF mutein-containing solutions thus obtained are brought into contact with crosslinked polysaccharide sulfate gel. For this purpose, both batch and column methods may be used.
The column method is however more suitable due to its simple operation. In the case of the column method, the crosslinked polysaccharide sulfate gel is filled, into a column, and thereafter to equilibrate the column it is thoroughly washed with a suitable buffer solution such as 50 mM citrate buffer (pH 7.0) containing 0.4 M NaCl. The amount of the gel to be used depends on the nature of the loaded hbFGF mutein-containing solution, but the range of about 1 to 50 ml per mg of hbFGF mutein is preferable.
The hbFGF mutein-containing solutions described above are then loaded on the column. The loading speed is selected in the space velocity (SV) range of about 0.1 to 5.0. After loading, the column is thoroughly washed, and the ionic strength of the buffer solution is increased by conventional methods to elute and recover the hbFGF muteins. In order to increase the ionic strength, salts such as NaCl are added or buffer solutions high in concentration are used so that the electric conductivity is increased to at least about 15 mU, preferably at least 30 mU*. For elution, both batch and concentration gradient elution methods may be
used. When the concentration gradient elution method is used, for example, the concentration of NaCl is gradually increased from about 0 M to 2.0 M, thereby conducting elution and recovery. Thus, highly purified hbFGF muteins can be obtained in high yield.
Examples of the synthetic polymers having sulfonic acid groups as exchange groups which are used in the present invention include polymers in which sulfonic acid groups are directly or. indirectly introduced into hydrophilic vinyl polymers, styrene-divinylbenzene polymers, acrylamide polymers and the like. In particular, SP (sulfopropyl)- Toyopearl (Tosoh, Japan) in which sulfonic groups are introduced into the hydrophilic vinyl polymer, is preferably used from the viewpoints of recovery and operation. When chromatographed, the hbFGF mutein-containing solutions partially purified are adjusted to about 50 mM or less in salt concentration, for example, in the case of phosphate buffer, and allowed to be adsorbed on the above resins within the pH range of about 5 to 7. For adsorption, both batch and column systems may be used, but the column system is preferably used from the viewpoint of operation. Elution from the resins is carried out by increasing the salt concentration. For elution, both batch and concentration gradient methods may be used. When the batch method is used, for example, a buffer solution prepared by adding NaCl to the above phosphate buffer to a concentration of about 500 mM to 1 M can be used. In lieu of the
phosphate buffer, citrate buffer can be used. The elution is conducted at a temperature of about 1 to 25 C, preferably ' about 1 to 10°C, more preferably about 4 C.
Examples of the synthetic polymers for gel filtration used in the present invention include hydrophilic vinyl polymers and acrylamide polymers. In particular, Toyopearl HW (Tosoh, Japan), the hydrophilic vinyl polymer, is preferably used from the viewpoints of gel durability and operation. When the hbFGF mutein-containing solutions' partially purified are treated, for example, on a column of Toyopearl HW-50F, buffer solutions such as phosphate buffer and citrate buffer can be used as developing solvents. It is however advantageous to use 50 mM citrate buffer (pH 7.0). The treatment is conducted at a temperature of about 1 to 25 C, preferably about 1 to 10°C, more preferably 'about 4 C.
A method other than the above methods may be used as one of the purifying procedures. In such methods, for example, natural products such as cellulose, agarose and dextran and inorganic materials such as glass beads can also be used as carriers for ion exchange chromatography.
As carriers for gel filtration, there can be similarly used gels mainly composed of natural products such as cellulose, agarose and dextran and gels based on inorganic materials such as glass beads.
The samples thus obtained can .also be dialyzed and lyophilized to form dried powders. Further, it is suitable
to add serum albumin as a carrier to the samples to store them, because the samples can be prevented from being adsorbed on vessels.
The coexistence with trace amounts of reducing agents in the course of purification or storage is suitable to prevent the samples from being oxidized. The reducing agents include S-mercaptoethanol, dithiothreitol and glutathione.
According to the present invention, substantially pure hbFGF muteins, essentially free from pyrogens and endotoxins can be obtained. The substantially pure hbFGF muteins according to the present invention include products which contain the hbFGF muteins according to the present invention in an amount of 95% (w/w) or more as protein content, more preferably in an amount of 98% (w/w) or more. The hbFGF muteins obtained by the above methods of the present invention have fibroblast growth promoting activity, vascular endothelial cell growth promoting activity and angiogenic activity, are high in stability and have low toxicity. They can therefore be used as healing promoters for burns, wounds, postoperative tissues and the like, or as therapeutic medicines based on their angiogenic activity for thrombosis, arteriosclerosis and the like. They can also be used as reagents for promoting cell cultivation. In particular, the mutein in which at least one of constituent cysteine residues is replaced by a serine residue is preferable because of their high stability.
When the hbFGF muteins according to the present invention are used as pharmaceutical preparations, they can be safely administered parenterally or orally to warm¬ blooded animals (such as humans, mice, rats, hamsters, rabbits, dogs and cats), in a powder form as such, or as pharmaceutical compositions (such as injections, tablets, capsules, solutions and ointments) with pharmacologically acceptable carriers, excipients and diluents.
The injections are prepared by conventional methods using, for example, physiological saline or aqueous solutions containing glucose or other auxiliary agents. The pharmaceutical compositions such as tablets and capsules can also be prepared in accordance with conventional methods. When the hbFGF muteins according to the present invention are used as the above pharmaceutical preparations, they are administered, for example, to the above warm¬ blooded animals in an appropriate amount ranging from about 1 ng/kg body weight to 100 μg/kg body weight daily, taking into account the route of administration, symptoms, etc. Further, when the hbFGF muteins according to the present invention are used as the reagents for accelerating cell cultivation, they are preferably added to culture media so as to be contained in an amount of about 0.01 to 10 μg per liter of medium, more preferably in an amount of about o.l to 10 μg per liter of medium.
When bases, amino acids and so on are indicated by the abbreviations in this specification and the drawings, the
abbreviations adopted by IUPAC-IUB Commission on Biochemical Nomenclature or commonly used in the art are employed. For example, the following abbreviations are used. When the optical isomer is capable of existing with respect to the amino acids, the L-form is represented unless otherwise specified.
DNA : Deoxyribonucleic acid cDNA : Complementary deoxyribonucleic acid
A : Adenine T : Thymine
G . : Guanine
C .: Cytosine
RNA : Ribonucleic acid dATP : Deoxyadenosine triphosphate dTTP : Deoxythymidine triphosphate dGTP : Deoxyguanosine triphosphate dCTP : Deoxycytidine triphosphate
ATP : Adenosine triphosphate
Tdr : Thymidine EDTA : Ethylenediaminetetraacetic acid
SDS : Sodium dodecyl sulfate
Gly : Glycine
Ala : Alanine
Val : Valine Leu : Leucine -
Ile : Isoleucine
Ser : Serine
Thr : Threonine-
Cys : Cysteine
Met : Methionine
Glu : Glutamic acid Asp : Arginine
Lys : Lysine
Arg : Arginine
His : Histidine
Phe : Phenylalanine Tyr : Tyrosine
Trp : Tryptophan
Pro : Proline
Asn : Asparagine
Gin : Glutamine Referring to Fig. 1, with respect to the number of the hbFGF-constituent amino acids, Met of the N-terminμs of the amino acid sequence is numbered as the 1st. In this specification, however, Pro next to the Met is numbered as the 1st. Transformant E^_ coli MM294/pTB762 carrying plasmid pTB762 used in Example 1 described below, transformant E. coli MM294(DE3)/pTB960 produced in Example 1, transformant E. coli MM294(DE3)/pCM901 produced in Example 8 and E. coli DHl/pTBl004 which harbors plasmid pTBl004 (Example 1) have been deposited with the Institute for Fermentation, Osaka (IF0), Japan, and with the Fermentation Research Institute, Agency of Industrial Science and Technology, Ministry of
International Trade and Industry (FRI) , Japan. Their accession numbers and deposit dates are shown in Table 1. The deposit of E. coli MM294/pTB762 in FRI was initially made under accession numbers denoted by FERM P numbers. Said deposit was converted to the deposit under the Budapest Treaty and the transformants have been stored at FRI under accession numbers denoted by FERM BP numbers.
Table 1
The present invention will hereinafter be described in detail with the following Examples. It is understood of course that these are not intended to limit the scope of the invention.
Best Mode for Carrying Out the Invention Example 1
Preparation of rhbFGF Mutein CS23 Production Recombinant
Plasmid pTB762 containing .a gene coding for rhbFGF mutein CS23 in which the cysteine residues at positions 69
and 87 of four cysteine residues existing in hbFGF were replaced by serine residues [Senoo et al., Biochemical and - Biophysical Research Communication 151, 701- 708 (1988), European Patent Publication No. 281,822] was treated with EcoRI and PstI to cut out a DNA fragment coding for rhbFGF mutein CS23. Then, plasmid pTBl004 (European Patent Publication No. 336,383) was completely digested with EcoRI and PstI, and thereafter this fragment was ligated to the- resulting largest fragment by T4 DNA ligase to construct plasmid pTB921. Then, the plasmid pTB921 was Cleaved with restriction enzyme EcoRI, and mungbean (yaenari) nuclease was reacted with the product to change the termini thereof to flush ends, followed by cleavage with restriction enzyme Bglll to prepare a DNA fragment containing the gene coding for rhbFGF mutein CS23. Additionally, vector pET3C carrying a > 10 promoter for a T7 phage [F. W. Studier et al.. Journal of Molecular Biology 189, 113-130 (1986)] was cleaved with restriction enzyme Ndel,' and the product was treated with mungbean nuclease to change the termini thereof to flush ends, followed by reaction with restriction enzyme BamHl. A DNA fragment containing rhbFGF mutein CS23 was ligated to the resulting DNA fragment by T4 ligase to obtain expression plasmid pTB960 (Fig. 2).
E. coli μM294 was lysogenized with ^ hage DE3 in which an RNA polymerase gene of the T7 phage was inserted [F. W. Studier et al.. Journal of Molecular Biology 189, 113-130 (1986)] to prepare E_;_ coli strain MM294(DE3). This
E. coli strain was transformed by using the expression plasmid pTB960, thereby obtaining recombinant
E. coli MM294(DE3)/pTB960 (IFO 14979, FERM BP-2690) carrying the gene coding for rhbFGF mutein CS23 shown in Fig. 1. Example 2
30 ml of LB medium (10 g/1 of bactotryptone, 5 g/1 of bactoyeast extract and 5 g/1 of NaCl) containing 50 mg/1 of sodium ampicillin was inoculated with one loopful of the recombinant E^_ coli MM294(DE3)/pTB960 (IFO 14979, FERM BP- 2690), and incubated at 37°C overnight with shaking. 1.5 ml portions of this culture solution were transferred to 30 ml of M-9 medium (16.8 g/1 of Na2HP04'12H-0, 3 g/1 of KH.PO^ 1 g/1 of NH.C1, 0.5 g/1 of NaCl and 0.246g/l of MgS0 '7H 0) . supplemented with 15 g/1 of glucose, 15 g/1 of casamino acids, 1 mg/1 of thiamine hydrochloride and 50 mg/1 of sodium ampicillin, and the cultivation with shaking was carried out at 37 C. When the culture was grown to a Klett unit of 100 to 120 in turbidity, IPTG was added to obtain various concentrations and the cultivation was further continued for 4 hours. Cells were collected by centrifugation and stored at -20 C.
Two identical samples were prepared. To one sample was added 7 M guanidine hydrochloride, and the cells were sufficiently loosened, followed by standing for 1 hour. Then, a supernatant was obtained by centrifugation (total amount of bFGF mutein) . To the other sample was added a 50 μg/ml lysozyme solution (10% sucrose, 10 mM EDTA, 100 mM
NaCl, 1 mM APMSF and 10 mM Tris-HCl, pH 7.6), and the mixture was allowed to stand at 4°C for 1 hour. Then, the cells were disrupted by ultrasonication (Kubota Insonater 200 M), followed by centrifugation to obtain a supernatant (amount of soluble hbFGF mutein) . Both the extracted solutions were diluted, and then the amount of rhbFGF mutein CS23 was determined using an ELISA. The results are shown in Table 2.
Table 2
Amount of Amount of accumulated Formation ratio of added IPTG rhbFGF mutein CS23 soluble rhbFGF mutein (μM) (mg/1) CS23 (%)
400 35 13
100 41 12
75 62 25
50 73 28
25 81 32
6.3 96 60
3.2 15 80
1.6 ζ 5
As is shown above, in the case of small-scale cultivation such as cultivation in flasks, the effect of the present invention can be obtained in the range of small amounts of added IPTG. Namely, about 1 mM of IPTG was added to induce the lac promoter. When IPTG was added in an amount of 100 μM or more, the productivity was low and most of the desired product was accumulated in an insoluble
state. In contrast, according to the present invention in which IPTG was added within a range from 3 μM to 100 μM, especially 6 μM to 80 μM, ther were obtained the entirely unexpected results of significant improvements in productivity and also in ratio of the mutein accumulated as a soluble protein. Example 3
M-9 medium (pH 6.8) containing 15 g/1 of glucose, 15 g/1 of casamino acids and' 5 mg/1 of thiamine hydrochloride was placed in an amount of 2.5 liter in a 5 liter jar fermentor, and sterilized. Then, the medium was inoculated with 125 ml of a strain culture solution prepared in the manner described in Example 2, followed by cultivation adjusting the pH to 6.8, at 37°C, with aeration at a rate of 2.5 1/min and with stirring at 1,000 rp . When the turbidity reached 120 Klett units during cultivation (3 hours after the initiation of cultivation) , IPTG was added so as to be contained in an amount of 10 μM. 8 hours after the initiation of cultivation, glucose and casamino acids were further added thereto so as to be contained in an amount of 10 g/1, repectively. The cultivation was further continued for 15 hours as a whole. As a result, 440 mg/1 of rhbFGF mutein CS23 was accumulated in a soluble state. In contrast, when 400 μM of IPTG was added under the same conditions, the production of rhbFGF mutein CS23 was only 17.5 mg/1.
Example 4
A seed culture solution prepared in the manner described in Example 2 was transferred to a 5 liter jar fermentor in which the same medium as with Example 3 was placed, and the cultivation was initiated at 30 C, at pH 6.8, with aeration at a rate of 2.5 1/min and with stirring at 1,000 rpm. When the turbidity reached about 700 Klett units 7 hours after the initiation of cultivation, 42 μM of IPTG was added. 8.5 hours after the initiation of cultivation, 20 g/1 of glucose and 20 g/1 of casamino acids were added at pH 6.8. The cultivation was carried out for 36 hours. In the culture solution, 860 mg/1 of rhbFGF mutein CS23 was accumulated in a soluble state. Example 5 M-9 medium (pH 6.8) containing 15 g/1 of glucose, 15 g/1 of casamino acids and 5 mg/1 of thiamine hydrochloride was placed in an amount of 2.5 liter in a 5 liter jar fermentor, and sterilized. Then, iron ions were added thereto at concentrations shown in Table 3 after sterilization by filtration. The resulting medium was inoculated with 125 ml portions of a strain culture solution prepared in the manner described in Example 2, followed by cultivation adjusting the pH to 6.8, at 30°C, with aeration at a rate of 2.5 1/min and with stirring at 1,000 rpm. When the culture was grown to a Klett unit of about 300 in turbidity (about 5.5 to 6 hours after the initiation of cultivation) , IPTG was added so as to be contained in an
amount of 42 μM, and at the same time the cultivation temperature was lowered to 25°C, followed' by cultivation for 23.5 hours. 7 to 7.5 hours after the initiation of cultivation, each of glucose and casamino acids was added in a ratio of 15 g/1, and the pH was maintained at 6.8 during cultivation. The results are shown in Table 3.
Table 3
Amount of added Productivity of rhbFGF mutein iron ion CS23 o : IToo
2X10"6 M 1.42
9X10"6 M 2.60
3.6X10"5 M 2.06
1X10 4 M 1.23
(Note: The amount of added iron ions indicates the amount of iron ions further added to the medium. The productivity indicates the weight ratio taking as 1 when the amount of added iron ions is 0.) Example 6
1 liter of LB medium containing 50 mg/1 of sodium ampicillin was inoculated with 1 ml of the master cells of recombinant E-_ coli MM294(DE3)/pTB960 (IFO 14979, FERM BP- 2690), and the cultivation with shaking was carried out at 30 C for 8 hours. The total amount thereof was transferred to 20 liter of LB medium (containing 50 mg/1 of sodium ampicillin) placed in a 50 liter culture tank, and the
cultivation was carried out at 30 C, under an inner pressure of 1.0 kg/cm G, with aeration at a rate of 10 1/min and with stirring at 300 rpm for 7 hours. Then, 18 liters of the resulting culture solution was transferred to 360 liter of a fermentation medium (M-9 medium containing 15 g/1 of glucose, 15 g/1 of casamino acids, 5 mg/1 of vitamin B. and
5 mg/1 of FeSO.* 7H-.0), and the cultivation was initiated at
30 C, under an inner pressure of 1.0 kg/cm G, with aeration at a rate of 240 1/min and with stirring at 250 rpm. When the culture was grown to a Klett unit of about 1,000, IPTG was added in an amount corresponding to 100 μM, and the cultivation temperature was lowered to 25°C. At the same time, a 50 liter sterilized solution containing 18 kg of glucose and 5.4 kg of casamino acids was added at a rate of about 2 1/hour, and then the cultivation was continued for 36 hours. As a result, 1.2 g/1 of rhbFGF mutein CS23 was accumulated in a soluble state. The cultivated product thus obtained was subjected to a Sharpless centrifuge to collect wet cells, and the cells were frozen at -80 C to store them. Example 7
Purification of rhbFGF Mutein CS23
1 kg of the cells (E_;_ coli MM294(DE3)/pTB960) freeze- stored at -80°C in Example 6 was suspended in 4 liter of a buffer containing 25 mM phosphate buffer (pH 6.0), 0.1 mM p-amidinophenylmethanesulfonyl fluoride hydrochloride (APMSF) and 2 mM dithiothreitol (DTT). The cells were disrupted 5 cycles under ice cooling with a 5 liter Dynomill
Model KD-5 (Willybachfen, Switzerland) using 4 liter of glass beads. The glass beads were washed with about 5 liter of the buffer, and the washings were collected together with the extract. About 10 li'ter of the resulting solution was' centrifuged at 10,000 rpm (Model 21, Beckman, U.S.A.) for 60 minutes to give a supernatant. The supernatant thus obtained was poured into a column (25.2 cm ID X 50 cm) of Sulfated Cellulofine (Seikagaku Kogyo, Japan). After adsorption, the column was washed with 25 liter of 25 mM phosphate buffer (pH 7.0) containing 0.5 M NaCl. Then, elution was effected using 30 liter of 25 mM phosphate buffer (pH 7.0) containing 1 M NaCl. The eluate was concentrated using an ultrafilter (Pellicon Casset System, Millipore, U.S.A.) until the absorbance at 280 nm became 3 to 4. The resulting concentrated solution was dialyzed against about 60 liter of 25 mM phosphate buffer (pH 6.0) overnight. The dialysate was centrifuged with a centrifuge (Beckman, U.S.A.) at 4,200 rpm for 30 minutes to give a supernatant. The supernatant was poured into a column (17.0 cm ID X 33.0 cm) of SP-Toyopearl 650M (Tosoh, Japan). The column was washed with 7 liter of 25 mM phosphate buffer (pH 6.0) containing 200 mM NaCl, and then elution was conducted using 10 liter of 25 mM phosphate buffer (pH 6.0) containing 500 mM NaCl. The eluate was poured into a column (15.0 cm ID X 50.0 cm) of Sulfated Cellulofine (Seikagaku Kogyo) using a high performance liquid chromatography apparatus (Gilson, France). Then, elution was effected by a linear
gradient between 20 liter of 50 mM citrate buffer (pH 7.0) and 30 liter of 50 mM citrate buffer (pH 7.0) containing 2 M NaCl. The main fractions were collected, and diluted with 10 mM citrate buffer (pH 6.0) to a conductivity of 20 mmho or less. The diluted solution was poured into a column
(14.0 cm-ID X 39.0 cm) of SP-Toyopearl 650 M (Tosoh, Japan). The column was washed with' 10 liter of 25 mM citrate buffer (pH 6.0), and then elution was conducted by using 10 liter of 25 mM citrate buffer (pH 6.0) containing 0.5 M NaCl. The eluate was poured into a column (14.0 cm ID X 90.0 cm) of Toyopearl HW-50F (Tosoh, Japan), and developed by using 50 mM citrate buffer (pH 7.0). The main fractions were collected, and sterilized by passing through a Millipak 20 filter (Millipore, U.S.A.) to provide a purified stock solution of rhbFGF mutein CS23. The yield was 17.0 g (the percent yield was 70%). The results of N-terminal. amino acid sequence analysis, C- terminal amino acid analysis and amino acid composition analysis are shown in Tables 4 to 6, respectively.
Table 4 N-terminal amino acid sequence analysis
Amino acid residue (pmol) Amino acid deduced rhbFGF mutein CS23 from nucleotide
Cycle No. (750 pmol). sequence
__
Ala Leu Pro Glu Asp Gly Gly Ser Gly Ala Phe Pro Pro Gly His Phe Lys Asp
Pro
* Analyzed with a Model 477A protein sequencer (Applied Biosystems)
Table 5 C-terminal amino acid analysis
C-terminal amino acid Yield (%) Ser 41.0 * Hydrazinolysis method
** Analyzed with a Model 6330 amino acid analyzer (Beckman)
Table 6 Amino acid composition analysis
Amino acid rhbFGF mutein CS23 Theoretical value
Asp/Asn 12
Thr 5
Ser 12 Glu/Gln 12
Pro 9
Gly 15
Ala 9
Cys 2'
Val 7
Met 2
Ile 4
Leu 13
Tyr 7
Phe 8
His 3
Lys 14
- Not assayed
* Hydrolysis method with hydrochloric acid ** Analyzed with a Model 6330 amino acid analyzer (Beckman) 1) Edelhoch method
Further, Fig. 3 shows the results of SDS-PAGE obtained under reducing conditions (100 mM. DTT, 50 C, 15 minutes). Referring to Fig. 3, (1) and (2) represent the results for the marker and rhbFGF mutein CS23, respectively. Fig. 4 shows the result of high performance liquid chromatography using a column (7.5 mm ID X 7.5 cm) having heparin-5PW (Tosoh, Japan) as a carrier under the following conditions:
Eluents: A solution [50 mM phosphate buffer (pH 7) ] B solution [50 mM phosphate buffer (pH 7) containing 2 M NaCl] Flow rate: 0.8 ml/mi Detection wave length: 280 nm
Fig. 5 shows the result of high performance liquid chromatography using a column (4.6 mm ID X 150 cm) having ODP-50 (Asahi Chemical Industry, Japan) as a carrier under the following conditions:
Eluents: A solution (0.1% trifluoroacetic acid)
B solution (80% acetonitrile containing 0.1% trifluoroacetic acid)
Flow rate: 1.0 l/min Detection wave length: 280 nm
Fig. 6 shows the result of high performance liquid chromatography using a column (7.5 mm ID X 7.5 cm) having G2000SW (Tosoh, Japan) as a carrier under the following conditions:
Developing solution: 0.1 M phosphate buffer (pH 7)
+ 0.1 M sodium sulfate
Flow rate: 0.5 ml/min
Detection wave length: 280 nm The biological activity was assayed by the method in which the growth promoting activity of fetal calf cardiac endothelial cells was given as an indication of the biological activity. As a result, an activity equivalent to that of the sample was shown. Example 8
Preparation of rhbFGF Mutein CS23 Production
Recombinant Having a Tetracycline Resistant Marker
Plasmid pTB762 containing a gene coding for rhbFGF mutein CS23 in which the cysteine residues at positions 69 and 87 of four cysteine residues existing in hbFGF were replaced by serine residues [Senoo et al., Biochemical and
Biophysical Research Communication 151, 701- 708 (1988),
European Patent Publication No. 281,822] was completely digested with Aval and PstI to obtain a DNA fragment of about 0.45 kbase pairs containing most of rhbFGF mutein CS23. A synthetic DNA ( GATCTGC ) was ligated to
ACGTCTAGACG the resulting fragment with T4DNA ligase, and then the resultant was digested with Aval and PstI to obtain a fragment A in which a PstI cleavage site was modified to a
Bglll cleavage site. Thereafter, plasmid pTB955 into which a gene coding for rhbFGF mutein C128 being deleted
C-terminus of hbFGF [Seno et al., European Journal of
Biochemistry, 188, 239-245 (1990)] was completely digested with Sail and BamHl to obtain a fragment B of about 4.1 kbase pairs. pTB955 was completely digested with Aval and Sail to obtain a fragment- C of about 390 base pairs. The three fragments A, B and C were ligated with T4DNA ligase each other to obtain an expression plasmid pHP901 containing an ampicillin resistant marker (Fig. 7). pHP901 was completely digested with EcoRI and Bglll to obtain a fragment of about 1.1 kbases containing a gene coding for rhbFGF mutein CS23, T7 promoter and T7 terminator. The fragment was ligated to pUClδ completely digested with EcoRI and BamHl by T4DNA ligase to obtain plasmid pME901 (Fig. 8). pME901 was completely digested with EcoRV and Hindlll to obtain a fragment of about 0.77 kbases containing a gene coding for rhbFGF mutein CS23, T7 promoter and T7 terminator. The fragment was incubated with T4DNA polymerase to change the termini thereof to flush ends. The fragment was ligated to pBR322 digested with Seal by T4DNA ligase to obtain an expression plasmid pCM901 containing a tetracycline marker (Fig. 9).
E. coli μM294 was lysogenized with phage DE3 in which an RNA polymerase gene of the T7 phage was inserted [F. W. Studier et al.. Journal of Molecular Biology 189, 113-130 (1986)3 to prepare E. ' coli strain MM294(DE3). This E. coli strain was transformed by using the expression plasmid pCM901, thereby obtaining recombinant
E. coli MM294(DE3)/ρCM901 (IFO 15104, FERM BP-3168) carrying the gene coding for rhbFGF mutein CS23. Example 9
1 liter of LB medium containing 5 mg/1 of tetracycline hydrochloride was inoculated with 1 ml of the master cells of recombinant E^ coli MM294(DE3)/pCM901 (IFO 15104, FERM BP-3168), and the cultivation with shaking was carried .out at 30°C for 8 hours. The total amount thereof was transferred to 20 liter of LB medium (containing 5 mg/1 of tetracycline hydrochloride) placed in a 50 liter culture tank, and the cultivation was carried out at 30 C, under an
2 inner pressure of 1.0 kg/cm G, with aeration at a rate of 10
1/min and with stirring at 300 rpm up to a Klett unit of 700. Then, the total amount of the resulting culture solution was transferred to 360 liter of a fermentation medium (M-9 medium containing 15 g/1 of glucose, 15 g/1 of casamino acids, 16.8 g/1 of N 2HP04 *12H20, 3 g/1 of KH2P04, 1 g/1 of NH Cl, 0.5 g/1 of sodium chloride, 0.5 g/1 of MgSO *7H70, 5 mg/1 of thiamin hydrochloride, 2.5 mg/1 of FeSO.* 7H20 and 0.2 g/1 of antifoaming agent), and the cultivation was initiated at 28°C, under an inner pressure of 1.0 kg/cm G, with aeration at a rate of 240 1/min and with stirring at 250 rpm. When the culture was grown to a Klett unit of about 450, 10 mg/1 (42 μM) of IPTG was added thereto. Since the remaining sugar was about 1%, a solution containing a mixture of glucose (60 g/1) and casein hydrolysate (15 g/1) was fed and the cultivation was
continued for 40 hours. As a result, 1.6 g/1 of rhbFGF mutein CS23 was accumulated in a soluble state. The cultivated product thus obtained was subjected to a Sharpless centrifuge to collect wet cells, and the cells were frozen at -80 C to store them.
1 kg of the obtained cells were treated by the manner similar to Example 7 to obtain a purified rhbFGF mutein CS23 original solution (yield 25.0 g) . Quality of the solution was equal to that of the sample obtained in Example 7 in both of a biological activity and physical and chemical assay.
Incorporated by reference
Nature 2 9, 123 (1974)
National Cancer Institute Monograph .48., 109 (1978)
The EMBO Journal 5_, 2523-2528 (1986)
FEBS Letters 213, 189-194 (1987)
The Journal of Biological Chemistry 263, 16471-16478 (1988)
The Journal of Biological Chemistry 26 > 16297-16302 (1988)
Biochemical and Biophysical Research Communication 151,
701-708 (1988) European Patent Publication No. 281,822 Journal of Molecular Biology 18_9, 113-130 (1986) Biotechnology and Applied Biochemistry, !L2, 436-449 (1990) Biochemical and Biophysical Research Communication 151, 701-708 (1988)
Genetic Engineering, p.31-50, Academic Press (1983)
Genetic Engineering: Principles and Methods, vol.3, p.1-32, Plenum Press (1981)
Proc. Natl. Acad. Sci. U.S.A. 74, 4266-4270 (1977)
Cell 16., 815-825 (1979)
Nature .280.' 35 (1979)
J . Mol . Biol . 18ji , 113-130 ( 1986 )
Gene 56., 125-135 ( 1987 )
Proc. Natl. Acad. Sci. U.S.A. 6.9, 2110 (1972)
Gene 17, 107 (1982)
Journal of Experiments in Molecular Genetics, 431-433, Cold Spring Harbor Laboratory, New York, 1972
Cell, 57, 881-889 (1989)
Gene, 8, 345-355 (1988)
DNA, 5 , 21-28 (1986)
European Journal of Biochemistry, 188, 239-245 (1990)