US5962225A - Methods and compositions for detection of specific nucleotide sequences - Google Patents
Methods and compositions for detection of specific nucleotide sequences Download PDFInfo
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- US5962225A US5962225A US08/739,069 US73906996A US5962225A US 5962225 A US5962225 A US 5962225A US 73906996 A US73906996 A US 73906996A US 5962225 A US5962225 A US 5962225A
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- nucleic acid
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
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- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Abstract
Description
TABLE 1 __________________________________________________________________________ Properties of some mammalian nucleases Mode of Reaction Mol. Enzyme Substrate action.sup.1 pH.sup.2 Mg.sup.1 product.sup.3 wt. __________________________________________________________________________ DNAse I ds/ss DNA Endo 7.1 + 5' oligos 31 Kdal DNAse II ds/ss DNA Endo 4.1 - 3' oligos 38 Kdal DNAse III ss Duplex DNA Exo 8.5 + 5' monodinu- 52 Kdal nucleotides DNAse IV Duplex DNA Exo 3'5' 8.5 + 5' mono 42 Kdal DNAse V Duplex DNA Exo 8.8 + 5' mono 12 Kdal 3'5'/5'3' DNAse VI ssDNA Endo 9.5 + 5' oligo 45 Kdal DNAse VII ss and nickel & Exo 3'5' 7.8 + 5' mono 43 Kdal dsDNA DNA VIII 5' ss and nickel Exo 5'3' 9.5 + 5' oligos 31 Kdal Correxo ss DNA nickel Exo 8.0 + 5' oligos 30-35 UV'd ds DNA.sup.4 3'5'/5'3' Kdal Lysosomal or RNA or DNA Exo 5'3' 5.5 - 3' mono 70 Kdal spleen with 5' OH exonuclease __________________________________________________________________________ .sup.1 Endo = endonucleolytic. Exo = exonucleolytic .sup.2 optimum pH .sup.3 Oligonucleotide shown is the main reaction product. oligos = oligonucleotides mono = mononucleotides .sup.4 UV irradiated double stranded DNA
5'-TTT TCT TTT CCC CCC T-3'
5'-ACT GCC ATT TGT ACT GCT GT-Dig-3'
5'-GAA TAG TAG ACA TAA TAG TA-FITC-3'
TFO: 5'-TCC GCC TTT TGT TGT TTT TC-3'
5'-CCA GGC AAA TCT ACT GAA ACG CTG-Dig-3'
5'-TAG ACA AGC TTG AGC TTA AAG-FITC-3'
5'-GGG GCG ACG ACG GGT GAC GGG G-3'
5'-TCT GAC CTA TCA GCT TTG GAC GGT-Dig-5'
5'-TAG ATG TGG TAG CCG TTT CTC AGG-FITC-3'
5'-CCA TTT TTC CCT GAG CTT TTT-3'
5'-TAA TTC TTC AGA GTT AAT GGG A-Dig-5'
5'-AAC ATG AAG ATG GCT ATC GTG TC-FITC-3'
______________________________________ Target Nucleic Acid Protection Molecule ______________________________________ ss DNA ssDNA ss DNA with crosslinkers ss DNA that covers restriction sites with homologous bases/non-cross linking ss DNA with crosslinkers and 5' non-homologous tail ss RNA with crosslinkers ss RNA with non-homologous RNA tail on either side of target ss RNA with crosslinkers ss RNA ss RNA + Antibody to hybrid ss RNA with non-complementary RNA tail (either one or both sides of target sequence).sup.1 ss RNA with non-complementary RNA tail, 3' side target.sup.1 ss DNA ss RNA with non-complementary RNA tail, 5' side target.sup.1 ss DNA extending beyond flanking regions and covering restriction sites.sup.3 ss DNA homologous to target site.sup.3 ss DNA complementary to target ss RNA with non-complementary DNA tail (either one or both sides of target sequence).sup.1 ss RNA with non-complementary DNA tail, 3' side target.sup.1 ss RNA with non-complementary DNA tail, 5' side target ss DNA with non-complementary RNA tail (either one or both sides of target sequence) ss DNA with non-complementary RNA tail, 3' side target ss DNA with non-complementary RNA tail, 5' side target ss DNA with non-complementary 5' DNA tail ss RNA + DNA ss DNA ss RNA with non-complementary RNA tail, 5' side target.sup.1 ss DNA target specific only with TFO ss DNA with non-complementary 5'tail.sup.2 ss RNA with non-complementary RNA tail on both sides of target + antibody to hydbrid ss RNA with non-complementary RNA tail on both sides of target ss RNA with non-complementary 5'tail.sup.2 ss RNA with non-complementary 5' DNA tail ds DNA TFO DNA or RNA PNA PNA with non-complementary 5' DNA tail Sequence specific protein ss RNA PNA ss DNA + Antibody to DNA/RNA hybrid ss RNA.sup.3 ss DNA.sup.3 ss RNA with cross linkers ss Dna with cross linkers ss RNA Sequence specific protein ds RNA TFO (RNA) TFO (DNA) PNA ______________________________________ .sup.1 +Antibody to DNA/RNA hybrid can also be used to aid in binding .sup.2 binding aided by pH and ionic strength .sup.3 PNA can also be added.
__________________________________________________________________________ # SEQUENCE LISTING - (1) GENERAL INFORMATION: - (iii) NUMBER OF SEQUENCES: 15 - (2) INFORMATION FOR SEQ ID NO:1: - (i) SEQUENCE CHARACTERISTICS: #pairs (A) LENGTH: 16 base (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear - (ii) MOLECULE TYPE: cDNA - (iii) HYPOTHETICAL: YES - (iv) ANTI-SENSE: NO - (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1: # 16 - (2) INFORMATION FOR SEQ ID NO:2: - (i) SEQUENCE CHARACTERISTICS: #pairs (A) LENGTH: 20 base (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear - (ii) MOLECULE TYPE: cDNA - (iii) HYPOTHETICAL: YES - (iv) ANTI-SENSE: NO - (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2: # 20 CTGT - (2) INFORMATION FOR SEQ ID NO:3: - (i) SEQUENCE CHARACTERISTICS: #pairs (A) LENGTH: 20 base (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear - (ii) MOLECULE TYPE: cDNA - (iii) HYPOTHETICAL: NO - (iv) ANTI-SENSE: NO - (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3: # 20 AGTA - (2) INFORMATION FOR SEQ ID NO:4: - (i) SEQUENCE CHARACTERISTICS: #pairs (A) LENGTH: 20 base (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear - (ii) MOLECULE TYPE: cDNA - (iii) HYPOTHETICAL: YES - (iv) ANTI-SENSE: NO - (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4: # 20 TTTC - (2) INFORMATION FOR SEQ ID NO:5: - (i) SEQUENCE CHARACTERISTICS: #pairs (A) LENGTH: 24 base (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear - (ii) MOLECULE TYPE: cDNA - (iii) HYPOTHETICAL: YES - (iv) ANTI-SENSE: NO - (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5: # 24AAAC GCTG - (2) INFORMATION FOR SEQ ID NO:6: - (i) SEQUENCE CHARACTERISTICS: #pairs (A) LENGTH: 21 base (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear - (ii) MOLECULE TYPE: cDNA - (iii) HYPOTHETICAL: YES - (iv) ANTI-SENSE: NO - (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6: #21 TAAA G - (2) INFORMATION FOR SEQ ID NO:7: - (i) SEQUENCE CHARACTERISTICS: #pairs (A) LENGTH: 18 base (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear - (ii) MOLECULE TYPE: cDNA - (iii) HYPOTHETICAL: YES - (iv) ANTI-SENSE: NO - (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7: # 18 GC - (2) INFORMATION FOR SEQ ID NO:8: - (i) SEQUENCE CHARACTERISTICS: #pairs (A) LENGTH: 23 base (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear - (ii) MOLECULE TYPE: cDNA - (iii) HYPOTHETICAL: YES - (iv) ANTI-SENSE: NO - (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8: # 23GCTC CTC - (2) INFORMATION FOR SEQ ID NO:9: - (i) SEQUENCE CHARACTERISTICS: #pairs (A) LENGTH: 24 base (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear - (ii) MOLECULE TYPE: cDNA - (iii) HYPOTHETICAL: YES - (iv) ANTI-SENSE: NO - (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9: # 24ATAC TGAT - (2) INFORMATION FOR SEQ ID NO:10: - (i) SEQUENCE CHARACTERISTICS: #pairs (A) LENGTH: 22 base (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear - (ii) MOLECULE TYPE: cDNA - (iii) HYPOTHETICAL: YES - (iv) ANTI-SENSE: NO - (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10: # 22CGG GG - (2) INFORMATION FOR SEQ ID NO:11: - (i) SEQUENCE CHARACTERISTICS: #pairs (A) LENGTH: 24 base (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear - (ii) MOLECULE TYPE: cDNA - (iii) HYPOTHETICAL: YES - (iv) ANTI-SENSE: NO - (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11: # 24TGGA CGGT - (2) INFORMATION FOR SEQ ID NO:12: - (i) SEQUENCE CHARACTERISTICS: #pairs (A) LENGTH: 24 base (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear - (ii) MOLECULE TYPE: cDNA - (iii) HYPOTHETICAL: YES - (iv) ANTI-SENSE: NO - (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12: # 24TTCT CAGG - (2) INFORMATION FOR SEQ ID NO:13: - (i) SEQUENCE CHARACTERISTICS: #pairs (A) LENGTH: 21 base (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear - (ii) MOLECULE TYPE: cDNA - (iii) HYPOTHETICAL: YES - (iv) ANTI-SENSE: NO - (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13: #21 TTTT T - (2) INFORMATION FOR SEQ ID NO:14: - (i) SEQUENCE CHARACTERISTICS: #pairs (A) LENGTH: 22 base (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear - (ii) MOLECULE TYPE: cDNA - (iii) HYPOTHETICAL: YES - (iv) ANTI-SENSE: NO - (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14: # 22TGG GA - (2) INFORMATION FOR SEQ ID NO:15: - (i) SEQUENCE CHARACTERISTICS: #pairs (A) LENGTH: 23 base (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear - (ii) MOLECULE TYPE: cDNA - (iii) HYPOTHETICAL: YES - (iv) ANTI-SENSE: NO - (xi) SEQUENCE DESCRIPTION: SEQ ID NO:15: # 23TCGT GTC __________________________________________________________________________
Claims (18)
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/739,069 US5962225A (en) | 1995-10-27 | 1996-10-25 | Methods and compositions for detection of specific nucleotide sequences |
US09/313,121 US6100040A (en) | 1995-10-27 | 1999-05-17 | Methods and compositions for detection of specific nucleotide sequences |
US09/633,848 US6458540B1 (en) | 1995-10-27 | 2000-08-07 | Methods and compositions for detection of specific nucleotide sequences |
US09/992,128 US20020119475A1 (en) | 1995-10-27 | 2001-11-05 | Methods and compositions for detection of specific nucleotide sequences |
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US593895P | 1995-10-27 | 1995-10-27 | |
US08/739,069 US5962225A (en) | 1995-10-27 | 1996-10-25 | Methods and compositions for detection of specific nucleotide sequences |
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US09/313,121 Continuation US6100040A (en) | 1995-10-27 | 1999-05-17 | Methods and compositions for detection of specific nucleotide sequences |
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US5962225A true US5962225A (en) | 1999-10-05 |
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US08/739,069 Expired - Fee Related US5962225A (en) | 1995-10-27 | 1996-10-25 | Methods and compositions for detection of specific nucleotide sequences |
US09/313,121 Expired - Fee Related US6100040A (en) | 1995-10-27 | 1999-05-17 | Methods and compositions for detection of specific nucleotide sequences |
US09/633,848 Expired - Fee Related US6458540B1 (en) | 1995-10-27 | 2000-08-07 | Methods and compositions for detection of specific nucleotide sequences |
US09/992,128 Abandoned US20020119475A1 (en) | 1995-10-27 | 2001-11-05 | Methods and compositions for detection of specific nucleotide sequences |
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US09/313,121 Expired - Fee Related US6100040A (en) | 1995-10-27 | 1999-05-17 | Methods and compositions for detection of specific nucleotide sequences |
US09/633,848 Expired - Fee Related US6458540B1 (en) | 1995-10-27 | 2000-08-07 | Methods and compositions for detection of specific nucleotide sequences |
US09/992,128 Abandoned US20020119475A1 (en) | 1995-10-27 | 2001-11-05 | Methods and compositions for detection of specific nucleotide sequences |
Country Status (8)
Country | Link |
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US (4) | US5962225A (en) |
EP (1) | EP0864002A4 (en) |
JP (1) | JPH11513887A (en) |
KR (1) | KR19990067080A (en) |
AU (1) | AU730154B2 (en) |
CA (1) | CA2235804A1 (en) |
MX (1) | MX9803308A (en) |
WO (1) | WO1997015691A1 (en) |
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US6458540B1 (en) | 1995-10-27 | 2002-10-01 | Cygene, Inc. | Methods and compositions for detection of specific nucleotide sequences |
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US6623920B1 (en) * | 1999-07-09 | 2003-09-23 | Gen-Probe Incorporated | Detection of HIV-1 by nucleic acid amplification |
US20030186310A1 (en) * | 2000-10-26 | 2003-10-02 | Kincaid Robert H. | Apparatus and methods of detecting features on a microarray |
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Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4775619A (en) * | 1984-10-16 | 1988-10-04 | Chiron Corporation | Polynucleotide determination with selectable cleavage sites |
EP0492570A1 (en) * | 1990-12-24 | 1992-07-01 | Enzo Diagnostics, Inc. | Method for detecting a target polynucleotide in a sample using a background reducing reagent and composition and kit comprising such a reagent |
US5176996A (en) * | 1988-12-20 | 1993-01-05 | Baylor College Of Medicine | Method for making synthetic oligonucleotides which bind specifically to target sites on duplex DNA molecules, by forming a colinear triplex, the synthetic oligonucleotides and methods of use |
US5273881A (en) * | 1990-05-07 | 1993-12-28 | Daikin Industries, Ltd. | Diagnostic applications of double D-loop formation |
US5482836A (en) * | 1993-01-14 | 1996-01-09 | The Regents Of The University Of California | DNA purification by triplex-affinity capture and affinity capture electrophoresis |
US5518901A (en) * | 1993-04-19 | 1996-05-21 | Murtagh; James J. | Methods for adapting nucleic acid for detection, sequencing, and cloning using exonuclease |
US5639609A (en) * | 1993-06-09 | 1997-06-17 | Boehringer Mannheim Gmbh | Method for immobilizing nucleic acids using modified capture probes |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5258506A (en) | 1984-10-16 | 1993-11-02 | Chiron Corporation | Photolabile reagents for incorporation into oligonucleotide chains |
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
FR2596773B1 (en) | 1986-04-04 | 1988-06-24 | Pasteur Institut | NON-RADIOACTIVE MARKED SINGLE-STRANDED PROBE, METHOD FOR THE PRODUCTION THEREOF AND METHOD FOR DETECTING A DETERMINED NUCLEOTIDE SEQUENCE USING THE SAME |
US5422251A (en) | 1986-11-26 | 1995-06-06 | Princeton University | Triple-stranded nucleic acids |
JP2739378B2 (en) * | 1990-11-09 | 1998-04-15 | アメリカ合衆国 | Method for targeting DNA |
US5607924A (en) | 1992-01-21 | 1997-03-04 | Pharmacyclics, Inc. | DNA photocleavage using texaphyrins |
WO1993018187A1 (en) * | 1992-03-13 | 1993-09-16 | California Institute Of Technology | Triple helix recognition of dna |
US5583032A (en) | 1992-10-21 | 1996-12-10 | The Cleveland Clinic Foundation And National Institutes Of Health | Method of cleaving specific strands of RNA |
US5422253A (en) | 1992-12-07 | 1995-06-06 | Wisconsin Alumni Research Foundation | Method of site specific nucleic acid cleavage |
US5726297A (en) | 1994-03-18 | 1998-03-10 | Lynx Therapeutics, Inc. | Oligodeoxyribonucleotide N3' P5' phosphoramidates |
US5599922A (en) | 1994-03-18 | 1997-02-04 | Lynx Therapeutics, Inc. | Oligonucleotide N3'-P5' phosphoramidates: hybridization and nuclease resistance properties |
US5714328A (en) | 1995-06-07 | 1998-02-03 | Board Of Regents, The University Of Texas System | RNA photocleavage using texaphyrins |
EP0864002A4 (en) | 1995-10-27 | 1999-07-28 | Elliot R Ramberg | Methods and compositions for detection of specific nucleotide sequences |
US5834198A (en) | 1996-03-21 | 1998-11-10 | Boehringer Mamnnheim Gmbh | Selective photoinducted flavin-dependent cleavage of RNA at G-U base pairs and kits therefor |
-
1996
- 1996-10-25 EP EP96942723A patent/EP0864002A4/en not_active Withdrawn
- 1996-10-25 US US08/739,069 patent/US5962225A/en not_active Expired - Fee Related
- 1996-10-25 JP JP9516836A patent/JPH11513887A/en not_active Ceased
- 1996-10-25 WO PCT/US1996/017191 patent/WO1997015691A1/en not_active Application Discontinuation
- 1996-10-25 KR KR1019980703020A patent/KR19990067080A/en not_active Application Discontinuation
- 1996-10-25 AU AU11568/97A patent/AU730154B2/en not_active Ceased
- 1996-10-25 CA CA002235804A patent/CA2235804A1/en not_active Abandoned
-
1998
- 1998-04-27 MX MX9803308A patent/MX9803308A/en unknown
-
1999
- 1999-05-17 US US09/313,121 patent/US6100040A/en not_active Expired - Fee Related
-
2000
- 2000-08-07 US US09/633,848 patent/US6458540B1/en not_active Expired - Fee Related
-
2001
- 2001-11-05 US US09/992,128 patent/US20020119475A1/en not_active Abandoned
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4775619A (en) * | 1984-10-16 | 1988-10-04 | Chiron Corporation | Polynucleotide determination with selectable cleavage sites |
US5176996A (en) * | 1988-12-20 | 1993-01-05 | Baylor College Of Medicine | Method for making synthetic oligonucleotides which bind specifically to target sites on duplex DNA molecules, by forming a colinear triplex, the synthetic oligonucleotides and methods of use |
US5273881A (en) * | 1990-05-07 | 1993-12-28 | Daikin Industries, Ltd. | Diagnostic applications of double D-loop formation |
EP0492570A1 (en) * | 1990-12-24 | 1992-07-01 | Enzo Diagnostics, Inc. | Method for detecting a target polynucleotide in a sample using a background reducing reagent and composition and kit comprising such a reagent |
US5482836A (en) * | 1993-01-14 | 1996-01-09 | The Regents Of The University Of California | DNA purification by triplex-affinity capture and affinity capture electrophoresis |
US5518901A (en) * | 1993-04-19 | 1996-05-21 | Murtagh; James J. | Methods for adapting nucleic acid for detection, sequencing, and cloning using exonuclease |
US5639609A (en) * | 1993-06-09 | 1997-06-17 | Boehringer Mannheim Gmbh | Method for immobilizing nucleic acids using modified capture probes |
Non-Patent Citations (48)
Title |
---|
Actor et al. "A Bioluminescent Assay Using AquaLite for RT-PCR Amplified RNA from Mouse Lung", The Journal of NIH Research, vol. 8, p. 62 (Oct. 1996). |
Actor et al. A Bioluminescent Assay Using AquaLite for RT PCR Amplified RNA from Mouse Lung , The Journal of NIH Research, vol. 8, p. 62 (Oct. 1996). * |
Aviv et al., "Purification of Biologically Active Globin Messenger RNA by Chromatograply on Oligothymidylic acid-Cellulose", Proc. Nat. Acad. Sci., vol. 69, No. 6, pp. 1408-1412 (Jun. 1972). |
Aviv et al., Purification of Biologically Active Globin Messenger RNA by Chromatograply on Oligothymidylic acid Cellulose , Proc. Nat. Acad. Sci., vol. 69, No. 6, pp. 1408 1412 (Jun. 1972). * |
Berger et al. editors Guide to Molecular Cloning Techniques Methods in Enzymology 152:612 613 & 625 626, 1987. * |
Berger et al. editors Guide to Molecular Cloning Techniques Methods in Enzymology 152:612-613 & 625-626, 1987. |
Blume et al., "Triple helix formation by purine-rich oligonucleotides targeted to the human dihydrofolate reductase promotor", Nucleic Acids Research, vol. 20, No. 7, pp. 1777-1784. |
Blume et al., Triple helix formation by purine rich oligonucleotides targeted to the human dihydrofolate reductase promotor , Nucleic Acids Research, vol. 20, No. 7, pp. 1777 1784. * |
Cassiday et al., "Replica Plating of Colonies from Listeria-Agars to Blood Agar to Improve the Isolation of Listeria monocytogenes from Foods" Applied and Environmental Microbiology, vol. 56, No. 7, Jul., pp. 2274-2275 (1990). |
Cassiday et al., Replica Plating of Colonies from Listeria Agars to Blood Agar to Improve the Isolation of Listeria monocytogenes from Foods Applied and Environmental Microbiology, vol. 56, No. 7, Jul., pp. 2274 2275 (1990). * |
Chambers, Henry "Coagulase-Negative Staphylococci Resistant to β-Lactam Antibiotics in vivo Produce Penicillin-Binding Protein 2a", Antimicrobial Agents and Chemotherapy, vol. 31, No. 12, pp. 1919-1924 (Dec. 1987). |
Chambers, Henry Coagulase Negative Staphylococci Resistant to Lactam Antibiotics in vivo Produce Penicillin Binding Protein 2a , Antimicrobial Agents and Chemotherapy, vol. 31, No. 12, pp. 1919 1924 (Dec. 1987). * |
Chirgwin et al., "Isolation of Biologically Active Ribonucleic Acid from Sources Enriched in Ribonuclease", Biochemistry, vol. 18, No. 24, pp. 5294-5299 (1979). |
Chirgwin et al., Isolation of Biologically Active Ribonucleic Acid from Sources Enriched in Ribonuclease , Biochemistry, vol. 18, No. 24, pp. 5294 5299 (1979). * |
Chomczynski, et al., "Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction", Analytical Biochemistry, vol. 162, pp. 156-159 (1987). |
Chomczynski, et al., Single Step Method of RNA Isolation by Acid Guanidinium Thiocyanate Phenol Chloroform Extraction , Analytical Biochemistry, vol. 162, pp. 156 159 (1987). * |
Dhingra et al., "Hybridization Protection Assay: A Rapid, Sensitive, and Specific Method for Detection of Philadelphia Chromosome-Positive Leukemias", Blood, vol. 77, No. 2, pp. 238-242 (Jan. 15, 1991). |
Dhingra et al., Hybridization Protection Assay: A Rapid, Sensitive, and Specific Method for Detection of Philadelphia Chromosome Positive Leukemias , Blood, vol. 77, No. 2, pp. 238 242 (Jan. 15, 1991). * |
Favolaro, et al., "Transcription Maps of Polyoma Virus-Specific RNA: Analysis by Two-Dimensional Nuclease S1 Gel Mapping", Methods in Enzymology, vol. 65, pp. 718-749. |
Favolaro, et al., Transcription Maps of Polyoma Virus Specific RNA: Analysis by Two Dimensional Nuclease S1 Gel Mapping , Methods in Enzymology, vol. 65, pp. 718 749. * |
Goodwin, Graham H., "The analysis of sequence-specific DNA-binding proteins in cell extracts", Gel Electrophoresis of Nucleic Acids--A Practical Approach, Second Edition, Oxford University Press, pp. 225-247 (1990). |
Goodwin, Graham H., The analysis of sequence specific DNA binding proteins in cell extracts , Gel Electrophoresis of Nucleic Acids A Practical Approach, Second Edition, Oxford University Press, pp. 225 247 (1990). * |
Guatelli et al., "Isothermal in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication", Proc. Natl. Acad. Sci., vol. 87, pp. 1874-1878 (Mar., 1990). |
Guatelli et al., Isothermal in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication , Proc. Natl. Acad. Sci., vol. 87, pp. 1874 1878 (Mar., 1990). * |
Kolberg, et al., "The Specificity of pilin DNA sequences for the detection of pathogenic Neisseria", Molecular and Cellular Probes, vol. 3, pp. 59-72 (1989). |
Kolberg, et al., The Specificity of pilin DNA sequences for the detection of pathogenic Neisseria , Molecular and Cellular Probes, vol. 3, pp. 59 72 (1989). * |
Kwoh et al., "Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format", Proc. Natl. Acad. Sci., vol. 86, pp. 1173-1177 (Feb. 1989). |
Kwoh et al., Transcription based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead based sandwich hybridization format , Proc. Natl. Acad. Sci., vol. 86, pp. 1173 1177 (Feb. 1989). * |
Landegren et al., "DNA Diagnostics-Molecular Techniques and Automation", Science, vol. 242, pp. 229-237 (Oct. 14, 1988). |
Landegren et al., DNA Diagnostics Molecular Techniques and Automation , Science, vol. 242, pp. 229 237 (Oct. 14, 1988). * |
Lee et al., "Poly(pyrimidine)•poly(purine) synthetic DNAs containing 5-methylcytosine form stable triplexes at neutral pH", Nucleic Acids Research, vol. 12, No. 16, pp. 6603-6614 (1984). |
Lee et al., Poly(pyrimidine) poly(purine) synthetic DNAs containing 5 methylcytosine form stable triplexes at neutral pH , Nucleic Acids Research, vol. 12, No. 16, pp. 6603 6614 (1984). * |
Lee, et al., "Isolation of DNA from Fungal Mycelia and Single Spores", PCR Protocols: A Guide to Methods and Applications, Academic Press, Inc., pp. 282-287 (1990). |
Lee, et al., Isolation of DNA from Fungal Mycelia and Single Spores , PCR Protocols: A Guide to Methods and Applications, Academic Press, Inc., pp. 282 287 (1990). * |
Lewis, Ricky, "GEN's 10 Best Biotechnology Research Areas", Genetic Engineering News, vol. 10:1, p. 24 (1990). |
Lewis, Ricky, GEN s 10 Best Biotechnology Research Areas , Genetic Engineering News, vol. 10:1, p. 24 (1990). * |
Lizardi et al., "Exponential Amplification of Recombinant-RNA Hybridization Probes", Bio/Technology, vol. 6, pp. 1197-1202 (Oct. 1988). |
Lizardi et al., Exponential Amplification of Recombinant RNA Hybridization Probes , Bio/Technology, vol. 6, pp. 1197 1202 (Oct. 1988). * |
Palmiter, Richard D., "Magnesium Precipitation of Ribonucleoprotein Complexes. Expedient Techniques for the Isolation of Undegraded Polysomes and Messenger Ribonucleic Acid", Biochemistry, vol. 13, No. 17, pp. 3606-3615 (1974). |
Palmiter, Richard D., Magnesium Precipitation of Ribonucleoprotein Complexes. Expedient Techniques for the Isolation of Undegraded Polysomes and Messenger Ribonucleic Acid , Biochemistry, vol. 13, No. 17, pp. 3606 3615 (1974). * |
Reddy, et al., "Bacterial RNA Isolation with One Hour Centrifugation in a Table-Top Ultracentrifuge", BioTechniques, vol. 8, No. 3, pp. 250-251 (1990). |
Reddy, et al., Bacterial RNA Isolation with One Hour Centrifugation in a Table Top Ultracentrifuge , BioTechniques, vol. 8, No. 3, pp. 250 251 (1990). * |
Summers, William C., "A Simple Method for Extraction of RNA from E. coli Utilizing Diethyl Pyrocarbonate", Analytical Biochemistry, "Short Communications", 33(2), pp. 459-463 (1970). |
Summers, William C., A Simple Method for Extraction of RNA from E. coli Utilizing Diethyl Pyrocarbonate , Analytical Biochemistry, Short Communications , 33(2), pp. 459 463 (1970). * |
Urdea et al., "A novel method for the rapid detection of specific nucleotide sequences in crude biological samples without blotting or radioactivity; application to the analysis of hepatitis B virus in human serum", Gene, 61 pp. 253-264 (1987). |
Urdea et al., A novel method for the rapid detection of specific nucleotide sequences in crude biological samples without blotting or radioactivity; application to the analysis of hepatitis B virus in human serum , Gene, 61 pp. 253 264 (1987). * |
Wu, et al., "The Ligation Amplification Reaction (LAR-Amplification of Specific DNA Sequences Using Sequential Rounds of Template-Dependent Ligation" Genomics, vol. 4, pp. 560-569 (1989). |
Wu, et al., The Ligation Amplification Reaction (LAR Amplification of Specific DNA Sequences Using Sequential Rounds of Template Dependent Ligation Genomics, vol. 4, pp. 560 569 (1989). * |
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Also Published As
Publication number | Publication date |
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US6100040A (en) | 2000-08-08 |
EP0864002A4 (en) | 1999-07-28 |
US20020119475A1 (en) | 2002-08-29 |
AU730154B2 (en) | 2001-03-01 |
CA2235804A1 (en) | 1997-05-01 |
US6458540B1 (en) | 2002-10-01 |
AU1156897A (en) | 1997-05-15 |
JPH11513887A (en) | 1999-11-30 |
KR19990067080A (en) | 1999-08-16 |
WO1997015691A1 (en) | 1997-05-01 |
MX9803308A (en) | 1998-11-29 |
EP0864002A1 (en) | 1998-09-16 |
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