US3652222A - Bilirubin assay - Google Patents
Bilirubin assay Download PDFInfo
- Publication number
- US3652222A US3652222A US814161A US81416169A US3652222A US 3652222 A US3652222 A US 3652222A US 814161 A US814161 A US 814161A US 81416169 A US81416169 A US 81416169A US 3652222 A US3652222 A US 3652222A
- Authority
- US
- United States
- Prior art keywords
- bilirubin
- hydroxylamine
- assay
- azobilirubin
- set forth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/903—Diazo reactions
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/145555—Hetero-N
- Y10T436/146666—Bile pigment
Abstract
Description
Claims (8)
- 2. The method as set forth in claim 1, in which the acid solution of hydroxylamine is prepared by adding hydroxylamine hydrochloride to water.
- 3. The method as set forth in claim 1, in which the bilirubin assay is an alkaline azobilirubin procedure.
- 4. The method as set forth in claim 1, wherein the hydroxylamine is added to the reactant solution in place of ascorbic acid.
- 5. The method as set forth in claim 1, in which the acid hydroxylamine solution is prepared by dissolving an acid hydroxylamine salt in water.
- 6. In a method for colorimetric or spectrophotometric bilirubin assay, in which the color of azobilirubin formed from the coupling reaction of an azo reagent with bilirubin is measured, the improvement comprising using hydroxylamine in the reactant solution after the azobilirubin has formed therein, the hydroxylamine having been maintained stable prior to such use by being acidic.
- 7. The method as set forth in claim 6, in which the bilirubin assay is an alkaline azobilirubin procedure.
- 8. The method as set forth in claim 6, wherein the hydroxylamine is used in place of ascorbic acid.
- 9. In a method of bilirubin assay involving the measurement of the blue color of azobilirubin formed by the reaction of bilirubin and an azo reagent, the improvement comprising using hydroxylamine in a concentration which gives the same final concentration of hydroxylamine as when a solution which is 13 molar or greater of the hydroxylamine is substituted for ascorbic acid in a Jendrassik-Grof procedure.
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US814161A US3652222A (en) | 1969-04-07 | 1969-04-07 | Bilirubin assay |
JP45028536A JPS515600B1 (en) | 1969-04-07 | 1970-04-03 | |
GB1643170A GB1312712A (en) | 1969-04-07 | 1970-04-07 | Method of determining bilirubin |
DE2016555A DE2016555C3 (en) | 1969-04-07 | 1970-04-07 | Method for the colorimetric or photometric determination of bilirubin |
FR7012549A FR2043035A5 (en) | 1969-04-07 | 1970-04-07 | PROCEDURE FOR THE TEST AND TEST OF BILIRUBIN |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US814161A US3652222A (en) | 1969-04-07 | 1969-04-07 | Bilirubin assay |
Publications (1)
Publication Number | Publication Date |
---|---|
US3652222A true US3652222A (en) | 1972-03-28 |
Family
ID=25214328
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US814161A Expired - Lifetime US3652222A (en) | 1969-04-07 | 1969-04-07 | Bilirubin assay |
Country Status (5)
Country | Link |
---|---|
US (1) | US3652222A (en) |
JP (1) | JPS515600B1 (en) |
DE (1) | DE2016555C3 (en) |
FR (1) | FR2043035A5 (en) |
GB (1) | GB1312712A (en) |
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3850576A (en) * | 1972-06-19 | 1974-11-26 | Boehringer Mannheim Gmbh | Diagnostic composition for the detection of urobilinogen |
US3853476A (en) * | 1972-08-17 | 1974-12-10 | Boehringer Mannheim Gmbh | Diagnostic agent for the detection of bilirubin |
US3853466A (en) * | 1971-06-19 | 1974-12-10 | Boehringer Mannheim Gmbh | Diagnostic composition for the detection of urobilinogens |
US3880588A (en) * | 1972-08-17 | 1975-04-29 | Boehringer Mannheim Gmbh | Diagnostic agent for detecting bilirubin |
US4030885A (en) * | 1975-09-11 | 1977-06-21 | Sigma Chemical Company | Bilirubin determination |
US4078892A (en) * | 1975-06-30 | 1978-03-14 | Becton, Dickinson And Company | Novel means and method for diagnostic quantitation of serum or plasma bilirubin |
US4115064A (en) * | 1977-02-07 | 1978-09-19 | Pierce Chemical Company | Method for bilirubin determination |
US4260579A (en) * | 1979-05-10 | 1981-04-07 | Miles Laboratories, Inc. | Device and method for simulating bilirubin in urine |
US4336157A (en) * | 1980-06-27 | 1982-06-22 | Baxter Travenol Laboratories, Inc. | Process for reclaiming biliverdin-containing fluids |
WO1995000843A1 (en) * | 1993-06-18 | 1995-01-05 | Synermed, Inc. | Assay for total bilirubin |
US5599661A (en) * | 1993-12-28 | 1997-02-04 | Unitika, Ltd. | Reagent for measuring direct bilirubin |
US5804405A (en) * | 1996-11-27 | 1998-09-08 | Research Corporation Technologies, Inc. | Bilirubin detection |
WO1999004258A1 (en) * | 1997-07-17 | 1999-01-28 | Synermed International Inc. | Assay for total and direct bilirubin |
US5958781A (en) * | 1994-07-14 | 1999-09-28 | Abbott Laboratories | Methods and reagents for cyanide-free determination of hemoglobin and leukocytes in whole blood |
US20050266579A1 (en) * | 2004-06-01 | 2005-12-01 | Xihai Mu | Assay system with in situ formation of diazo reagent |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2301687A1 (en) * | 1973-01-13 | 1974-07-18 | Boehringer Sohn Ingelheim | METHOD FOR DETERMINING TOTAL BILIRUBIN IN BODY FLUIDS |
FR2280082A1 (en) * | 1974-07-25 | 1976-02-20 | Reveilleau R | Hydrazine-stabilized bilirubin colorimetric assay - by coupling of free or conjugated bilirubin with diazonium salts |
JP7153019B2 (en) * | 2016-12-14 | 2022-10-13 | エフ.ホフマン-ラ ロシュ アーゲー | Determination of interfering substances in samples |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3348920A (en) * | 1964-02-25 | 1967-10-24 | Dade Reagents Inc | Reagent and method for the quantitative determination of bilirubin |
US3511607A (en) * | 1967-10-06 | 1970-05-12 | Smithkline Corp | Laboratory reagent for assay of total bilirubin |
-
1969
- 1969-04-07 US US814161A patent/US3652222A/en not_active Expired - Lifetime
-
1970
- 1970-04-03 JP JP45028536A patent/JPS515600B1/ja active Pending
- 1970-04-07 FR FR7012549A patent/FR2043035A5/en not_active Expired
- 1970-04-07 GB GB1643170A patent/GB1312712A/en not_active Expired
- 1970-04-07 DE DE2016555A patent/DE2016555C3/en not_active Expired
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3348920A (en) * | 1964-02-25 | 1967-10-24 | Dade Reagents Inc | Reagent and method for the quantitative determination of bilirubin |
US3511607A (en) * | 1967-10-06 | 1970-05-12 | Smithkline Corp | Laboratory reagent for assay of total bilirubin |
Non-Patent Citations (2)
Title |
---|
Quigley, J. J., Analytical Chemistry, Vol. 24, pp. 1859 1860 (1952) * |
Welcher, F. J. ed., Standard Methods of Chemical Analysis, Vol. II, Part A., pp. 1081 1082 (1963) * |
Cited By (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3853466A (en) * | 1971-06-19 | 1974-12-10 | Boehringer Mannheim Gmbh | Diagnostic composition for the detection of urobilinogens |
US3850576A (en) * | 1972-06-19 | 1974-11-26 | Boehringer Mannheim Gmbh | Diagnostic composition for the detection of urobilinogen |
US3853476A (en) * | 1972-08-17 | 1974-12-10 | Boehringer Mannheim Gmbh | Diagnostic agent for the detection of bilirubin |
US3880588A (en) * | 1972-08-17 | 1975-04-29 | Boehringer Mannheim Gmbh | Diagnostic agent for detecting bilirubin |
US4078892A (en) * | 1975-06-30 | 1978-03-14 | Becton, Dickinson And Company | Novel means and method for diagnostic quantitation of serum or plasma bilirubin |
US4030885A (en) * | 1975-09-11 | 1977-06-21 | Sigma Chemical Company | Bilirubin determination |
US4115064A (en) * | 1977-02-07 | 1978-09-19 | Pierce Chemical Company | Method for bilirubin determination |
US4260579A (en) * | 1979-05-10 | 1981-04-07 | Miles Laboratories, Inc. | Device and method for simulating bilirubin in urine |
US4336157A (en) * | 1980-06-27 | 1982-06-22 | Baxter Travenol Laboratories, Inc. | Process for reclaiming biliverdin-containing fluids |
WO1995000843A1 (en) * | 1993-06-18 | 1995-01-05 | Synermed, Inc. | Assay for total bilirubin |
US5599661A (en) * | 1993-12-28 | 1997-02-04 | Unitika, Ltd. | Reagent for measuring direct bilirubin |
US5958781A (en) * | 1994-07-14 | 1999-09-28 | Abbott Laboratories | Methods and reagents for cyanide-free determination of hemoglobin and leukocytes in whole blood |
US6740527B1 (en) * | 1994-07-14 | 2004-05-25 | Abbott Laboratories | Methods and reagents for cyanide-free determination of hemoglobin and leukocytes in whole blood |
US5804405A (en) * | 1996-11-27 | 1998-09-08 | Research Corporation Technologies, Inc. | Bilirubin detection |
WO1999004258A1 (en) * | 1997-07-17 | 1999-01-28 | Synermed International Inc. | Assay for total and direct bilirubin |
US6326208B1 (en) | 1997-07-17 | 2001-12-04 | Synermed International Inc. | Assay for total and direct bilirubin |
US20050266579A1 (en) * | 2004-06-01 | 2005-12-01 | Xihai Mu | Assay system with in situ formation of diazo reagent |
Also Published As
Publication number | Publication date |
---|---|
DE2016555C3 (en) | 1979-10-04 |
GB1312712A (en) | 1973-04-04 |
DE2016555A1 (en) | 1970-11-05 |
FR2043035A5 (en) | 1971-02-12 |
DE2016555B2 (en) | 1979-02-01 |
JPS515600B1 (en) | 1976-02-20 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
AS | Assignment |
Owner name: MERCHANTS NATIONAL BANK & TRUST COMPANY OF INDIANA Free format text: SECURITY INTEREST;ASSIGNOR:AMERICAN MONITOR CORPORATION,;REEL/FRAME:004101/0767 Effective date: 19821103 |
|
AS | Assignment |
Owner name: SECURITY PACIFIC BUSINESS CREDIT INC. 230 WEST MON Free format text: SECURITY INTEREST;ASSIGNOR:AMERICAN MONITOR CORPORATION AN IN CORP.;REEL/FRAME:004070/0799 Effective date: 19821103 |
|
PA | Patent available for licence or sale | ||
AS | Assignment |
Owner name: MERCHANTS NATIONAL BANK & TRUST COMPANY, INDIANAPO Free format text: RELEASED BY SECURED PARTY;ASSIGNOR:MERCHANTS NATIONAL BANK & TRUST COMPANY OF INDIANAPOLIS;REEL/FRAME:004339/0926 Effective date: 19841127 Owner name: MERCHANTS NATIONAL BANK & TRUST COMPANY,INDIANA Free format text: RELEASED BY SECURED PARTY;ASSIGNOR:MERCHANTS NATIONAL BANK & TRUST COMPANY OF INDIANAPOLIS;REEL/FRAME:004339/0926 Effective date: 19841127 |
|
AS | Assignment |
Owner name: FOOTHILL CAPITAL CORPORATION, A CORP. OF CA, ILLIN Free format text: SECURITY INTEREST;ASSIGNOR:SECURITY PACIFIC BUSINESS CREDIT, INC.;REEL/FRAME:004493/0285 Effective date: 19841130 |
|
AS | Assignment |
Owner name: NEUBERGER AND BERMAN (LENDER), 522 FIFTH AVENUE, N Free format text: SECURITY INTEREST;ASSIGNOR:AMERICAN MONITOR CORPORATION;REEL/FRAME:004610/0276 Effective date: 19860630 |