US20110305771A1 - Krill Extracts for Treatment of Cardiovascular Diseases - Google Patents
Krill Extracts for Treatment of Cardiovascular Diseases Download PDFInfo
- Publication number
- US20110305771A1 US20110305771A1 US13/216,694 US201113216694A US2011305771A1 US 20110305771 A1 US20110305771 A1 US 20110305771A1 US 201113216694 A US201113216694 A US 201113216694A US 2011305771 A1 US2011305771 A1 US 2011305771A1
- Authority
- US
- United States
- Prior art keywords
- acid
- krill
- marine
- liquid
- contents
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/612—Crustaceans, e.g. crabs, lobsters, shrimps, krill or crayfish; Barnacles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/01—Hydrocarbons
- A61K31/015—Hydrocarbons carbocyclic
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
- A61K31/07—Retinol compounds, e.g. vitamin A
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/12—Ketones
- A61K31/122—Ketones having the oxygen directly attached to a ring, e.g. quinones, vitamin K1, anthralin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/20—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/20—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
- A61K31/201—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids having one or two double bonds, e.g. oleic, linoleic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/20—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
- A61K31/202—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids having three or more double bonds, e.g. linolenic
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
- A61K31/215—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids
- A61K31/22—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids of acyclic acids, e.g. pravastatin
- A61K31/23—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids of acyclic acids, e.g. pravastatin of acids having a carboxyl group bound to a chain of seven or more carbon atoms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
- A61K31/215—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids
- A61K31/22—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids of acyclic acids, e.g. pravastatin
- A61K31/23—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids of acyclic acids, e.g. pravastatin of acids having a carboxyl group bound to a chain of seven or more carbon atoms
- A61K31/232—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids of acyclic acids, e.g. pravastatin of acids having a carboxyl group bound to a chain of seven or more carbon atoms having three or more double bonds, e.g. etretinate
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
- A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline
- A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
- A61K31/355—Tocopherols, e.g. vitamin E
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
- A61K31/575—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids substituted in position 17 beta by a chain of three or more carbon atoms, e.g. cholane, cholestane, ergosterol, sitosterol
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/66—Phosphorus compounds
- A61K31/683—Diesters of a phosphorus acid with two hydroxy compounds, e.g. phosphatidylinositols
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/66—Phosphorus compounds
- A61K31/683—Diesters of a phosphorus acid with two hydroxy compounds, e.g. phosphatidylinositols
- A61K31/685—Diesters of a phosphorus acid with two hydroxy compounds, e.g. phosphatidylinositols one of the hydroxy compounds having nitrogen atoms, e.g. phosphatidylserine, lecithin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/66—Phosphorus compounds
- A61K31/683—Diesters of a phosphorus acid with two hydroxy compounds, e.g. phosphatidylinositols
- A61K31/688—Diesters of a phosphorus acid with two hydroxy compounds, e.g. phosphatidylinositols both hydroxy compounds having nitrogen atoms, e.g. sphingomyelins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
- A61K33/30—Zinc; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/06—Antihyperlipidemics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P5/00—Drugs for disorders of the endocrine system
- A61P5/24—Drugs for disorders of the endocrine system of the sex hormones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/06—Antiarrhythmics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/12—Antihypertensives
Definitions
- This invention relates to multi-therapeutic extracts derived from krill and/or marine, which can prevent and/or treat several diseases.
- Krill is the common name for small, shrimp-like crustaceans, however not shrimp, that swarm in dense shoals, especially in Antarctic waters. It is one of the most important food source for fish, some kind of birds and especially for baleen whales as being an important source of protein. Krill is also a good source of omega-3 fatty acid, which are well known for their health benefits.
- krill and/or marine oil may be used for the treatment of autoimmune murine lupus and other autoimmune diseases and can also be used for treating cardiovascular diseases.
- the krill and/or marine oil used for these treatments has only conserved its omega-3 fatty acids as active ingredients, which is a very small part of all the active ingredients of the krill and/or marine itself. This fact reduces the potential of the krill and/or marine oil as a treatment for these diseases.
- the krill and/or marine oil comprises Eicosapentanoic acid, Docosahexanoic acid, Phosphatidylcholine, Phosphatidylinositol, Phosphatidylserine, Phosphatidylethanolamine, Sphingomyelin, a-tocopherol, all-trans retinol, Astaxanthin and flavonoid.
- the krill and/or marine oil comprises Eicosapentanoic acid, Docosahexanoic acid, Linolenic acid, Alpha-linolenic acid, Linoleic acid, Arachidonic acid, Oleic acid, palmitic acid, palmitoleic acid, stearic acid, nervonic acid, Phosphatidylcholine, Phosphatidylinositol, Phosphatidylserine, Phosphatidylethanolamine, Sphingomyelin, Cholesterol, Triglycerides, Monoglycerides, a-tocopherol, all-trans retinol, Astaxanthin, Canthaxanthin, ⁇ -carotene, flavonoid, Zinc, Selenium, sodium, potassium and calcium.
- the krill and/or marine oil comprises Eicosapentanoic acid, Docosahexanoic acid, Linolenic acid, Alpha-linolenic acid, Linoleic acid, Arachidonic acid, Oleic acid, palmitic acid, palmitoleic acid, stearic acid, Phosphatidylcholine, Phosphatidylinositol, Phosphatidylserine, Phosphatidylethanolamine, Sphingomyelin, Cholesterol, Triglycerides, Monoglycerides, a-tocopherol, all-trans retinol, Astaxanthin, Canthaxanthin, ⁇ -carotene, Zinc and Selenium.
- the diseases that can be treated and/or prevented by the method of the present invention are cardiovascular diseases, arthritis, skin cancer, diabetes, premenstrual syndrome and transdermal transport enhancement.
- compositions for the treatment and/or prevention and/or therapy of the previously mentioned diseases comprising a therapeutically effective amount of krill and/or marine oil in association with a pharmaceutically acceptable carrier.
- krill and/or marine oil for the treatment and/or prevention and/or therapy of the previously mentioned diseases.
- krill and/or marine oil for the manufacture of a medicament for the treatment and/or prevention and/or therapy of the previously mentioned diseases.
- krill and/or marine extract for prevention and/or treatment and/or therapy of several diseases.
- a multi-therapeutic oil extract free of enzyme is derived from krill and/or marine, found in any marine environment around the world, for example, the Antarctic ocean (euphasia superba), the Pacific ocean (euphasia pacifica), the Atlantic ocean, the Indian ocean, in particular coastal regions of Mauritius Island and/or Reunion Island of Madagascar, Canadian West Coast, Japanese Coast, St-Lawrence Gulf and Fundy Bay, and this oil extract is a free fatty acid lipid fraction.
- the extraction process can be described as the following:
- an organic solvent which can be alcohol, preferably ethanol, isopropanol or t-butanol, or esters of acetic acid, preferably ethyl acetate. This in order to extract the remaining soluble lipid fraction from the solid phase;
- step (f) Recovering a lipid rich fraction from the liquid phase obtained at step (e) by evaporation of the solvent present in the liquid phase.
- the active components of the enzyme-free krill and/or marine oil extract are:
- the Omega-3 are found in more than 30 g/100 g. ii) Omega-6: i. Linoleic acid: >0.9 g/100 g ii. Arachidonic acid: ⁇ 0.45 g/100 g, preferably ⁇ 0.6 g/100 g iii) Omega-9: i.
- Oleic acid >5 g/100 g iv) palmitic acid: >10 g/100 g v) palmitoleic acid: 0.08 g/100 g vi) stearic acid: >0.5 g/100 g
- Phosphatidylcholine >4.5 g/100 g
- Phosphatidylinositol >107 mg/100 g
- Phosphatidylserine >75 mg/100 g
- Phosphatidylethanolamine >0.5 g/100 g
- Sphingomyelin >107 mg/100 g
- the neutral lipids of the krill and/or marine extract also comprises: Diglycerides: >0.5 g/100 g
- vitamin E >1.0 IU/100 g all-trans retinol (vitamin A): >1500 IU/100 g ⁇ -carotene: >3000 ⁇ g/100 ml
- Astaxanthin >20 mg/100 g
- Canthaxanthin >2 mg/100 g
- the krill and/or marine extract also comprises: Flavonoids: >0.5 mg/100 g Sodium: ⁇ 500 mg/100 g Calcium: >0.1 mg/100 g Potassium: >50 mg/100 g Aluminum: ⁇ 8.5 mg/100 g Protein: >4 g/100 g Moisture and volatile matter: ⁇ 0.8%
- the extract contains less than 25 ppm of solvent residue from the extraction process.
- the oil has the following stability indexes: Peroxide value: ⁇ 0.1 (mEq/kg) Oil Stability index: ⁇ 0.1 after 50 hours at 97.8° C. Saponification index: 70-180 Iodine value: 60-130%
- Krill and/or marine oil has been shown to decrease cholesterol in vivo. It also inhibits platelet adhesion and plaque formation and reduces vascular endothelial inflammation in a patient. It can offer hypertension prophylaxis. It prevents oxidation of low-density lipoprotein. It may have an inhibitory effect on the secretion of VLDL due to increased intracellular degradation of apo B-100. It also offers a post-myocardial infarction prophylaxis because of its ability to decrease CIII apolipoprotein B, to decrease CIII non-apolipoprotein B lipoproteins and to increase antithrombin III levels.
- Krill and/or marine oil is suitable for prophylactic usage against cardiovascular disease in human where cardiovascular disease relates to coronary artery disease, hyperlipidemia, hypertension, ischemic disease (relating to angina, myocardial infarction, cerebral ischemia, shock without clinical or laboratory evidence of ischemia, arrhythmia)
- cardiovascular disease relates to coronary artery disease, hyperlipidemia, hypertension, ischemic disease (relating to angina, myocardial infarction, cerebral ischemia, shock without clinical or laboratory evidence of ischemia, arrhythmia)
- the patients were tested for LDL, HDL, Triglycerides, vital signs, CBC, SGOT/SGPT, ⁇ -GT, ALP, Urea, Creatine, Glucose, K + , Na + , Ca 2+ and total indirect bilirubin cholesterol before treatment and also at 2 months.
- Table 1 is showing the results obtained from the previously described tests:
- Krill and/or marine oil offers symptomatic relief for Arthritis where arthritis relates to adult arthritis, Still's disease, polyarticular or pauciarticular juvenile rheumatoid arthritis, rheumatoid arthritis, osteoarthritis because it has been shown that it provides a clinical improvement in decreasing the number of tender joints and of analgesics consumed daily by decreasing the production of Interleukin-8 and Interleukin-1 in human patients. Patients with a bleeding tendency or severe psychiatric disease were excluded from the study.
- the recommended dosage varies between 1 and 4.8 grams of pure krill extract per day.
- the inclusion criteria for the study are being aged between 50 and 65 years, both genders being admissible, having a clinical diagnosis of primary osteoarthritis (mild to moderate) 6 to 12 months prior to study enrollment including pain and stiffness, radiographic confirmation of illness prior to enrollment. It also include evidence of measurable symptoms of OA for at least 3 months prior to study enrollment requiring the use of acetaminophen, anti-inflammatory agents or opioid analgesics. Patients were asked to stop the use of all “pain-killers” the week prior to initiation of the trial for wash-out purposes.
- the Exclusion criteria were a severe osteoarthritis, unavoidable sustained use of NSAID's, aspirin or other medicines for anti-inflammatory use, use of topical analgesics within 4 weeks of randomization visit, steroid injection into either knee within past 3 months, initiation of physical therapy or muscle conditioning within 3 months, seafood allergies, use of anticoagulants or salicylates, alcohol consumption exceeding 3 mixed drinks per day, concurrent medical/arthritic disease that could confound or interfere with the evaluation of pain, prior surgery (including arthroscopy) of either knee, a known “secondary” cause of osteoarthritis.
- Evaluation was based on daily dose of NSAIDs and/or analgesics and/or SAARDs, number of painful joints, number or swollen joints, duration of morning stiffness, visual analog scale (0-100) WOMACscale and SF36. Preliminary results have been obtained after 2 months. The number of NSAIDs and/or analgesics and/or SAARDs required for daily functioning has been recorded at initiation and at 2 months after initiation.
- Results shown at Table 2 demonstrate the effect of an uptake of krill extract on the relief of arthritis.
- Krill and/or marine oil has been shown to be a skin cancer prophylactic because of its retinol anti-carcinogenic effect, Astaxanthin anti-carcinogenic effect and its phopholipid anti-carcinogenic effect.
- nude mice preferably on C57BL6 Nude Congenic Mice-B6NU-T (heterozygotes) because of their proven susceptibility to skin cancer.
- mice were formed as follows: 48 fish oil: 16 with oral supplementation (po) 16 with local application, 16 with po and local application; 48 krill and/or marine oil: 16 with po, 16 with local application, 16 with po and local application.
- the test was conducted as a randomized blind controlled trial (statistical significance p ⁇ 0.05). Half of the mice have been treated orally or topically or both with oil containing 100% by weight krill and/or marine oil and the other half have been treated the same way with fish oil.
- Nutrition was fat-free chow for the first week and was modified accordingly with the assigned group as described below for the following 2-20 weeks in the quantity of 1 ml of oil per day.
- mice were divided in six groups as follows:
- Group A fat-free chow with supplementation of fish oil (20% of total calories)
- Group B fat-free chow (100% of calories)+local application of fish oil 2 times per day
- Group C fat free chow with supplementation of fish oil (20% of total calories)+local application of soy oil 2 times per day
- Group D fat-free chow with supplementation of krill and/or marine oil (20% of total calories)
- Group E fat free chow (100% of calories)+local application of krill and/or marine oil 2 times per day
- Group F fat-free chow with supplementation or krill and/or marine oil (20% of total calories)+local application of krill and/or marine oil 2 times per day
- mice had been submitted to UVB radiation using a fluorescent test lamp, emission spectrum 270-400 nm during weeks 2-20.
- the essay were performed during 30 minutes of UVB exposure per day and the test lamp was at a distance of 30 cm from the mice.
- mice were anesthetized with ether and sacrificed. Skin was examined blind by pathologists for signs of carcinogenesis.
- Tables 3-8) are showing the results obtained about the incidence of cancer when ultra-violet radiations are administered to mice's skin during 5 weeks.
- Krill and/or marine oil enhances transdermal transportation as a substrate for dermatological topical therapeutic applications. It may be used in dermatological treatments via creams, ointments, gels, lotions and oils. It may also be used in various therapeutic applications such as relating to anesthesic, corticosteroids, anti-inflammatory, antibiotic and ketolytic functions.
- Table 9 shows results obtained on skin hydration following the method previously described.
- Table 10 shows results obtained from the use of krill oil to reduce the pain and mood changes associated with premenstrual syndrome in women.
- Krill oil extract was administered to 7 women during 2 months. The women were taking 6 capsules of krill extract per day, each capsule containing 800 mg of krill oil.
- a recommended daily intake of krill oil is of about 1 to 4.8 grams. All participants were advised to continue with their usual nutrition habits and to refrain from initiating any restrictions in their diet. No serious side effects were reported.
Abstract
Description
- 1. Field of the Invention
- This invention relates to multi-therapeutic extracts derived from krill and/or marine, which can prevent and/or treat several diseases.
- 2. Description of Prior Art
- Krill is the common name for small, shrimp-like crustaceans, however not shrimp, that swarm in dense shoals, especially in Antarctic waters. It is one of the most important food source for fish, some kind of birds and especially for baleen whales as being an important source of protein. Krill is also a good source of omega-3 fatty acid, which are well known for their health benefits.
- It is known in the art to use krill and/or marine enzymes for the treatment of a great variety of diseases in human and animals such as infections, inflammations, cancers, HIV/AIDS, pain, polyps, warts, hemorrhoids, plaque, wrinkles, thin hairs, allergic itch, anti-adhesion, eye disease, acne, cystic fibrosis and immune disorders including autoimmune disease and cancer.
- It is also known in the art that krill and/or marine oil may be used for the treatment of autoimmune murine lupus and other autoimmune diseases and can also be used for treating cardiovascular diseases.
- However, the krill and/or marine oil used for these treatments has only conserved its omega-3 fatty acids as active ingredients, which is a very small part of all the active ingredients of the krill and/or marine itself. This fact reduces the potential of the krill and/or marine oil as a treatment for these diseases.
- There is an increasing demand for treatments using products derived from a natural source, therefore, it would be highly desirable to be provided with a krill and/or marine extract having an enhanced potential for prevention and/or treatment and/or management of disease.
- In accordance with the present invention there is provided a method of prevention, therapy and/or treatment of several disease, the method comprising the administration of a therapeutically effective amount of krill and/or marine oil to a patient.
- In a preferred embodiment of the present invention the krill and/or marine oil is obtained from a process comprising the steps of:
- (a) placing krill and/or marine material in a ketone solvent, preferably acetone to achieve extraction of the soluble lipid fraction from the marine and/or aquatic animal material;
- (b) separating the liquid and solid contents;
- (c) recovering a first lipid rich fraction from the liquid contents by evaporation of the solvent present in the liquid contents;
- (d) placing the solid contents in an organic solvent selected from the group of solvents consisting of alcohol, preferably ethanol, isopropanol or t-butanol and esters of acetic acid, preferably ethyl acetate to achieve extraction of the remaining soluble lipid fraction from the marine and/or aquatic material;
- (e) separating the liquid and solid contents;
- (f) recovering a second lipid rich fraction by evaporation of the solvent from the liquid contents; and
- (g) recovering the solid contents.
- In a preferred embodiment of the present invention, the krill and/or marine oil comprises Eicosapentanoic acid, Docosahexanoic acid, Phosphatidylcholine, Phosphatidylinositol, Phosphatidylserine, Phosphatidylethanolamine, Sphingomyelin, a-tocopherol, all-trans retinol, Astaxanthin and flavonoid.
- In another embodiment of the present invention, the krill and/or marine oil comprises Eicosapentanoic acid, Docosahexanoic acid, Linolenic acid, Alpha-linolenic acid, Linoleic acid, Arachidonic acid, Oleic acid, palmitic acid, palmitoleic acid, stearic acid, nervonic acid, Phosphatidylcholine, Phosphatidylinositol, Phosphatidylserine, Phosphatidylethanolamine, Sphingomyelin, Cholesterol, Triglycerides, Monoglycerides, a-tocopherol, all-trans retinol, Astaxanthin, Canthaxanthin, β-carotene, flavonoid, Zinc, Selenium, sodium, potassium and calcium.
- In another embodiment of the present invention, the krill and/or marine oil comprises Eicosapentanoic acid, Docosahexanoic acid, Linolenic acid, Alpha-linolenic acid, Linoleic acid, Arachidonic acid, Oleic acid, palmitic acid, palmitoleic acid, stearic acid, Phosphatidylcholine, Phosphatidylinositol, Phosphatidylserine, Phosphatidylethanolamine, Sphingomyelin, Cholesterol, Triglycerides, Monoglycerides, a-tocopherol, all-trans retinol, Astaxanthin, Canthaxanthin, β-carotene, Zinc and Selenium.
- The diseases that can be treated and/or prevented by the method of the present invention are cardiovascular diseases, arthritis, skin cancer, diabetes, premenstrual syndrome and transdermal transport enhancement.
- In accordance with the present invention there is also provided a composition for the treatment and/or prevention and/or therapy of the previously mentioned diseases, the composition comprising a therapeutically effective amount of krill and/or marine oil in association with a pharmaceutically acceptable carrier.
- In accordance with the present invention, it is further provided the use of krill and/or marine oil for the treatment and/or prevention and/or therapy of the previously mentioned diseases.
- In accordance with the present invention, it is also provided the use of krill and/or marine oil for the manufacture of a medicament for the treatment and/or prevention and/or therapy of the previously mentioned diseases.
- In accordance with the present invention, there is provided krill and/or marine extract for prevention and/or treatment and/or therapy of several diseases.
- A multi-therapeutic oil extract free of enzyme is derived from krill and/or marine, found in any marine environment around the world, for example, the Antarctic ocean (euphasia superba), the Pacific ocean (euphasia pacifica), the Atlantic ocean, the Indian ocean, in particular coastal regions of Mauritius Island and/or Reunion Island of Madagascar, Canadian West Coast, Japanese Coast, St-Lawrence Gulf and Fundy Bay, and this oil extract is a free fatty acid lipid fraction.
- The extraction process can be described as the following:
- (a) Placing marine and/or aquatic krill and/or marine in a ketone solvent, preferably acetone, to achieve the extraction of grease from the krill and/or marine;
- (b) Separating the liquid and the solid phases;
- (c) Recovering a lipid rich fraction from the liquid phase obtained at step (b) by evaporation of the solvent present in the liquid phase;
- (d) Placing the solid phase in an organic solvent, which can be alcohol, preferably ethanol, isopropanol or t-butanol, or esters of acetic acid, preferably ethyl acetate. This in order to extract the remaining soluble lipid fraction from the solid phase;
- (e) Separating the liquid and the solid phases; and
- (f) Recovering a lipid rich fraction from the liquid phase obtained at step (e) by evaporation of the solvent present in the liquid phase.
- The active components of the enzyme-free krill and/or marine oil extract are:
- i. Eicosapentanoic acid: >8 g/100 g
ii. Docosahexanoic acid: >2 g/100 g
iii. Linolenic acid: >0.10 g/100 g
iv. Alpha-linolenic acid: >0.3 g/100 g
In the preferred embodiment of the present invention, the Omega-3 are found in more than 30 g/100 g.
ii) Omega-6: i. Linoleic acid: >0.9 g/100 g
ii. Arachidonic acid: <0.45 g/100 g, preferably <0.6 g/100 g
iii) Omega-9: i. Oleic acid: >5 g/100 g
iv) palmitic acid: >10 g/100 g
v) palmitoleic acid: 0.08 g/100 g
vi) stearic acid: >0.5 g/100 g - Phosphatidylcholine: >4.5 g/100 g
Phosphatidylinositol: >107 mg/100 g
Phosphatidylserine: >75 mg/100 g
Phosphatidylethanolamine: >0.5 g/100 g
Sphingomyelin: >107 mg/100 g - Cholesterol: <3 g/100 g
Triglycerides: <55 g/100 g
Monoglycerides: >0.5 g/100 g
In another embodiment of the present invention, the neutral lipids of the krill and/or marine extract also comprises:
Diglycerides: >0.5 g/100 g - α-tocopherol (vitamin E): >1.0 IU/100 g
all-trans retinol (vitamin A): >1500 IU/100 g
β-carotene: >3000 μg/100 ml - Astaxanthin: >20 mg/100 g
Canthaxanthin: >2 mg/100 g - Zinc: >0.1 mg/100 g
Selenium: >0.1 mg/100 g
In another embodiment of the present invention, the krill and/or marine extract also comprises:
Flavonoids: >0.5 mg/100 g
Sodium: <500 mg/100 g
Calcium: >0.1 mg/100 g
Potassium: >50 mg/100 g
Aluminum: <8.5 mg/100 g
Protein: >4 g/100 g
Moisture and volatile matter: <0.8% - After characterization of the krill and/or marine oil extract, it was determined that the extract contains less than 25 ppm of solvent residue from the extraction process.
- The oil has the following stability indexes:
Peroxide value: <0.1 (mEq/kg)
Oil Stability index: <0.1 after 50 hours at 97.8° C.
Saponification index: 70-180
Iodine value: 60-130% - The present invention will be more readily understood by referring to the following examples which are given to illustrate the invention rather than to limit its scope.
- Krill and/or marine oil has been shown to decrease cholesterol in vivo. It also inhibits platelet adhesion and plaque formation and reduces vascular endothelial inflammation in a patient. It can offer hypertension prophylaxis. It prevents oxidation of low-density lipoprotein. It may have an inhibitory effect on the secretion of VLDL due to increased intracellular degradation of apo B-100. It also offers a post-myocardial infarction prophylaxis because of its ability to decrease CIII apolipoprotein B, to decrease CIII non-apolipoprotein B lipoproteins and to increase antithrombin III levels. Krill and/or marine oil is suitable for prophylactic usage against cardiovascular disease in human where cardiovascular disease relates to coronary artery disease, hyperlipidemia, hypertension, ischemic disease (relating to angina, myocardial infarction, cerebral ischemia, shock without clinical or laboratory evidence of ischemia, arrhythmia)
- To evaluate the effects of krill and/or marine oil on the course of arteriosclerotic coronary artery disease and hyperlipidemia, a study was performed (prospective clinical trial, statistical significance p<0.05) with patients with known hyperlipidemia.
- A group of 13 patients took krill and/or marine oil concentrate gelules. Both fish oil and krill and/or marine oil contained equal amounts of omega-3 fatty acids. Recommended dosage is of 1 to 6 capsules per day, each capsule containing 800 mg of oil. In this study, each patient took 6 capsules per day.
- The patients were tested for LDL, HDL, Triglycerides, vital signs, CBC, SGOT/SGPT, γ-GT, ALP, Urea, Creatine, Glucose, K+, Na+, Ca2+ and total indirect bilirubin cholesterol before treatment and also at 2 months.
- Table 1 is showing the results obtained from the previously described tests:
-
TABLE 1 Paired Samples Test Paired Differences 95% Confidence Std. Interval of the Parameter Error Difference Sig. (2- tested Mean SD. Mean Lower Upper t-value df tailed) Cholesterol .4954 .55800 .15476 .1582 .8326 3.201 12 .008 Triglycerides .3538 .54543 .15127 .0242 .6834 2.339 12 .037 HDL −.2108 .29859 .08281 −.3912 −.0303 −2.545 12 .026 LDL .2846 .47333 .13128 −.0014 .5706 2.168 12 .051 Chol/HDL .3600 .53446 .14823 .0370 .6830 2.429 12 .032 - From the above, it was shown that a daily uptake of 1 to 4.8 g of krill extract was providing to the patients a cholesterol decrease in the range of 15%, a triglycerides decrease in the range of 15%, a HDL increase in the range of 8%, a LDL decrease in the range of 13% and a Cholesterol/HDL ratio decrease of 14%.
- This shows that an uptake of krill extract has a beneficial effect on patient suffering from hyperlipidemia, which is known to be the primary causative factor of atherosclerosis.
- Krill and/or marine oil offers symptomatic relief for Arthritis where arthritis relates to adult arthritis, Still's disease, polyarticular or pauciarticular juvenile rheumatoid arthritis, rheumatoid arthritis, osteoarthritis because it has been shown that it provides a clinical improvement in decreasing the number of tender joints and of analgesics consumed daily by decreasing the production of Interleukin-8 and Interleukin-1 in human patients. Patients with a bleeding tendency or severe psychiatric disease were excluded from the study.
- To evaluate the effects of krill and/or marine oil supplementation on the clinical course of osteoarthritis, a study was performed (prospective clinical trial, statistical significance p<0.05) with patients diagnosed with and treated for osteoarthritis which is Active class I, II or III and having treatment with NSAIDs and/or analgesics for at least 3 months before enrollment.
- A group of 13 patients took krill and/or marine oil concentrate capsules at a daily rate of 6 capsules of 800 mg krill oil per capsule. The recommended dosage varies between 1 and 4.8 grams of pure krill extract per day. Patients were asked to follow a normal healthy diet consisting of 20% fat (less than 10% animal fat), 40% protein and 40% carbohydrates.
- The inclusion criteria for the study are being aged between 50 and 65 years, both genders being admissible, having a clinical diagnosis of primary osteoarthritis (mild to moderate) 6 to 12 months prior to study enrollment including pain and stiffness, radiographic confirmation of illness prior to enrollment. It also include evidence of measurable symptoms of OA for at least 3 months prior to study enrollment requiring the use of acetaminophen, anti-inflammatory agents or opioid analgesics. Patients were asked to stop the use of all “pain-killers” the week prior to initiation of the trial for wash-out purposes.
- The Exclusion criteria were a severe osteoarthritis, unavoidable sustained use of NSAID's, aspirin or other medicines for anti-inflammatory use, use of topical analgesics within 4 weeks of randomization visit, steroid injection into either knee within past 3 months, initiation of physical therapy or muscle conditioning within 3 months, seafood allergies, use of anticoagulants or salicylates, alcohol consumption exceeding 3 mixed drinks per day, concurrent medical/arthritic disease that could confound or interfere with the evaluation of pain, prior surgery (including arthroscopy) of either knee, a known “secondary” cause of osteoarthritis.
- Evaluation was based on daily dose of NSAIDs and/or analgesics and/or SAARDs, number of painful joints, number or swollen joints, duration of morning stiffness, visual analog scale (0-100) WOMACscale and SF36. Preliminary results have been obtained after 2 months. The number of NSAIDs and/or analgesics and/or SAARDs required for daily functioning has been recorded at initiation and at 2 months after initiation.
- Results shown at Table 2 demonstrate the effect of an uptake of krill extract on the relief of arthritis.
-
TABLE 2 Frequency % Valid % Cumulative % No change 3 23.1 23.1 23.1 Pain relief 10 76.9 76.9 100.0 Total 13 100.0 100.0 - This shows that ten out of 13 (76.9%) people reported a significant pain relief and improvement of flexibility of large joints (lower back, knees, shoulders)
- Krill and/or marine oil has been shown to be a skin cancer prophylactic because of its retinol anti-carcinogenic effect, Astaxanthin anti-carcinogenic effect and its phopholipid anti-carcinogenic effect.
- To evaluate the photoprotective potential of krill and/or marine oil against UVB-induced skin cancer, a study was performed on nude mice, preferably on C57BL6 Nude Congenic Mice-B6NU-T (heterozygotes) because of their proven susceptibility to skin cancer.
- Groups were formed as follows: 48 fish oil: 16 with oral supplementation (po) 16 with local application, 16 with po and local application; 48 krill and/or marine oil: 16 with po, 16 with local application, 16 with po and local application. In order to establish efficacy of krill and/or marine oil for the prevention of skin cancer, the test was conducted as a randomized blind controlled trial (statistical significance p<0.05). Half of the mice have been treated orally or topically or both with oil containing 100% by weight krill and/or marine oil and the other half have been treated the same way with fish oil.
- Nutrition was fat-free chow for the first week and was modified accordingly with the assigned group as described below for the following 2-20 weeks in the quantity of 1 ml of oil per day.
- The mice were divided in six groups as follows:
- Group A: fat-free chow with supplementation of fish oil (20% of total calories)
Group B: fat-free chow (100% of calories)+local application of fish oil 2 times per day
Group C: fat free chow with supplementation of fish oil (20% of total calories)+local application of soy oil 2 times per day
Group D: fat-free chow with supplementation of krill and/or marine oil (20% of total calories)
Group E: fat free chow (100% of calories)+local application of krill and/or marine oil 2 times per day
Group F: fat-free chow with supplementation or krill and/or marine oil (20% of total calories)+local application of krill and/or marine oil 2 times per day - The mice had been submitted to UVB radiation using a fluorescent test lamp, emission spectrum 270-400 nm during weeks 2-20. The essay were performed during 30 minutes of UVB exposure per day and the test lamp was at a distance of 30 cm from the mice. At the end of the 20 weeks, or when malignant tumors had formed, mice were anesthetized with ether and sacrificed. Skin was examined blind by pathologists for signs of carcinogenesis.
- The following tables (Tables 3-8) are showing the results obtained about the incidence of cancer when ultra-violet radiations are administered to mice's skin during 5 weeks.
-
TABLE 3 Krill extract Oral uptake Cumulative Frequency Percent Valid Percent Percent Valid Benign 14 87.5 87.5 87.5 Cancer 2 12.5 12.5 100.0 Total 16 100.0 100.0 -
TABLE 4 Control Oral uptake Cumulative Frequency Percent Valid Percent Percent Valid Benign 14 87.5 87.5 87.5 Cancer 2 12.5 12.5 100.0 Total 16 100.0 100.0 -
TABLE 5 Krill extract topical uptake Cumulative Frequency Percent Valid Percent Percent Valid BENIGN 16 100.0 100.0 100.0 -
TABLE 6 Control topical uptake Cumulative Frequency Percent Valid Percent Percent Valid BENIGN 5 31.3 31.3 31.3 Cancer 11 68.8 68.8 100.0 Total 16 100.0 100.0 -
TABLE 7 Krill extract topical and oral uptake Cumulative Frequency Percent Valid Percent Percent Valid BENIGN 16 100.0 100.0 100.0 -
TABLE 8 Control topical and oral uptake Cumulative Frequency Percent Valid Percent Percent Valid BENIGN 10 62.5 62.5 62.5 Cancer 6 37.5 37.5 100.0 Total 16 100.0 100.0
The results obtained shows that both oral and topical use of krill oil is effective for the protection of the skin against the harmful effects of UVB radiation induced skin cancer. - Krill and/or marine oil enhances transdermal transportation as a substrate for dermatological topical therapeutic applications. It may be used in dermatological treatments via creams, ointments, gels, lotions and oils. It may also be used in various therapeutic applications such as relating to anesthesic, corticosteroids, anti-inflammatory, antibiotic and ketolytic functions.
- To evaluate the efficacy of krill and/or marine oil as a substrate for topical treatments and the speed of transdermal absorption of krill and/or marine alone or as a substrate, a study was performed as a randomized blind controlled trial on C57BL6 nude Congenic Mice-B6NU-T (heterozygotes).
- The results appearing in tables 5 and 6 are showing that topical treatment with krill oil faciliate the absorption of retinol and other antioxydants through the dermis which in turn result in significant photoprotective potential which in turn results in 100% protection from UVB induced skin cancer. In contrast, fish oil application with all-trans retinol resulted in 68.8% incidence of cancer.
- Krill and/or marine oil can be used to enhance transdermal transportation as a substrate for dermatological topical cosmetic applications where cosmetic applications relate to skin hydration, anti-wrinkle, keratolytics, peeling and mask via creams, ointments, gels, lotions or oils.
- To evaluate the effects of Krill and/or marine oil in aging and facial wrinkles, a study was conducted as a prospective clinical trial on patients concerned about facial dryness and wrinkles. Those patients had no prognosis severely limited by other dermatological or non-dermatological condition, bleeding tendency or severe psychiatric disease.
- 13 Healthy caucasian women with facial dryness or wrinkles have been included in this study. Women have been asked to take 6 capsules a day, each capsule containing 800 mg of krill extract. The recommended daily dosage is of about 1 to 4.8 g of krill extract.
- Table 9 shows results obtained on skin hydration following the method previously described.
-
TABLE 9 Changes in skin hydration Frequency % Valid % Cumulative % No change 4 30.8 30.8 30.8 Hydration 9 69.2 69.2 100.0 Total 13 100.0 100.0 - The results of the pilot study after 2 months indicate that nine out of 13 (69.2%) people reported a significant improvement of the hydration, texture and elasticity of the skin (face, hands and arms) in human patients.
- Moreover, these results are also indicative that krill extract is useful for anti-wrinkle treatment. The mechanism of all-trans retinol, which is included in the krill oil, as an anti-wrinkle works as follows:
- Regeneration and distinctive anti-inflammatory effects
Improve blood irrigation
Increases the epidermis regeneration by increasing the rate of cell division and turnover
Accelerates the differentiation of keratin
Regenerates the collagen
Allows cells in the top layer of the skin, which are always being replaces, to mature more normally than untreated sun-damaged cells
Reduces the activation of enzymes that break down the proteins collagen and elastin that provide structural support for the skin.
The results obtained with krill extract administered on a patent's skin show that the krill extract is having an anti-wrinkle effect by increasing the hydration and the mechanism above described. - Table 10 shows results obtained from the use of krill oil to reduce the pain and mood changes associated with premenstrual syndrome in women. Krill oil extract was administered to 7 women during 2 months. The women were taking 6 capsules of krill extract per day, each capsule containing 800 mg of krill oil. A recommended daily intake of krill oil is of about 1 to 4.8 grams. All participants were advised to continue with their usual nutrition habits and to refrain from initiating any restrictions in their diet. No serious side effects were reported.
- All women enrolled reported noticeable emotional and/or physical discomfort 7 to 10 days prior to menstruation. A self-assessment visual analogue scale validated for the assessment of the premenstrual syndrome, ranging from 0 (no symptoms) to 10 (unbearable) was used as a primary outcome in order to evaluate the effect of krill extract on premenstrual discomfort.
- Data analysis has been reported on 60% of the women participating in the study who have completed a two months regimen. The majority of the women (73.3%) showed a clinically significant reduction in both emotional and physical distress prior to menstruation (see Table 10).
-
TABLE 10 Frequency distribution of the effect of krill extract on premenstrual syndrome symptomatology PMS symptoms Frequency % Valid % Cumulative % No change 26.7 26.7 26.7 Positive 73.3 73.3 100.0 Total 100.0 100.0 - 8 human patients were taking krill extract at the dosage of 6 capsules a day, each capsule containing 800 mg of krill extract, during 2 months. A recommended daily intake of krill oil is of about 1 to 4.8 grams. The Table 11 is showing the variation in the glucose tested for the patients after 2 months.
-
TABLE 11 Variation in glucose in patients Paired Differences Std. 95% Confidence Parameter Error Interval of the Sig. (2- tested Mean SD. Mean Difference t-value df tailed) Glucose .5778 .60369 .20123 .1137-1.0418 2.871 8 .021
A blood glucose decrease of 20% was obtained for the patients taking krill extract, which shows that an uptake of krill extract is controlling blood glucose content and therefore controlling diabetes in human patients. - While the invention has been described in connection with specific embodiments thereof, it will be understood that it is capable of further modifications and this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains and as may be applied to the essential features hereinbefore set forth, and as follows in the scope of the appended claims.
Claims (57)
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US13/216,694 US20110305771A1 (en) | 2001-06-18 | 2011-08-24 | Krill Extracts for Treatment of Cardiovascular Diseases |
US14/152,603 US20140199414A1 (en) | 2001-06-18 | 2014-01-10 | Krill and/or marine extracts for prevention and/or treatment of cardiovascular diseases, arthritis, skin cancer, diabetes, premenstrual syndrome and transdermal transport |
US15/267,988 US20170224742A1 (en) | 2001-06-18 | 2016-09-16 | Phospholipid-containing marine extracts for treatment of cardiovascular diseases |
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US29838301P | 2001-06-18 | 2001-06-18 | |
US10/481,040 US20040241249A1 (en) | 2001-06-18 | 2002-06-07 | Krill and/or marine extracts for prevention and/or treatment of cardiovascular diseases arthritis, skin cancer diabetes, premenstrual syndrome and transdermal transport |
PCT/CA2002/000843 WO2002102394A2 (en) | 2001-06-18 | 2002-06-07 | Krill and/or marine extracts for prevention and/or treatment of cardiovascular diseases, arthritis, skin cancer, diabetes, premenstrual syndrome and transdermal transport |
US11/640,235 US8057825B2 (en) | 2001-06-18 | 2006-12-18 | Krill extracts for treatment of cardiovascular diseases |
US13/216,694 US20110305771A1 (en) | 2001-06-18 | 2011-08-24 | Krill Extracts for Treatment of Cardiovascular Diseases |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US11/640,235 Continuation US8057825B2 (en) | 2001-06-18 | 2006-12-18 | Krill extracts for treatment of cardiovascular diseases |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US14/152,603 Continuation US20140199414A1 (en) | 2001-06-18 | 2014-01-10 | Krill and/or marine extracts for prevention and/or treatment of cardiovascular diseases, arthritis, skin cancer, diabetes, premenstrual syndrome and transdermal transport |
Publications (1)
Publication Number | Publication Date |
---|---|
US20110305771A1 true US20110305771A1 (en) | 2011-12-15 |
Family
ID=23150259
Family Applications (5)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10/481,040 Abandoned US20040241249A1 (en) | 2001-06-18 | 2002-06-07 | Krill and/or marine extracts for prevention and/or treatment of cardiovascular diseases arthritis, skin cancer diabetes, premenstrual syndrome and transdermal transport |
US11/640,235 Expired - Fee Related US8057825B2 (en) | 2001-06-18 | 2006-12-18 | Krill extracts for treatment of cardiovascular diseases |
US13/216,694 Abandoned US20110305771A1 (en) | 2001-06-18 | 2011-08-24 | Krill Extracts for Treatment of Cardiovascular Diseases |
US14/152,603 Abandoned US20140199414A1 (en) | 2001-06-18 | 2014-01-10 | Krill and/or marine extracts for prevention and/or treatment of cardiovascular diseases, arthritis, skin cancer, diabetes, premenstrual syndrome and transdermal transport |
US15/267,988 Abandoned US20170224742A1 (en) | 2001-06-18 | 2016-09-16 | Phospholipid-containing marine extracts for treatment of cardiovascular diseases |
Family Applications Before (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10/481,040 Abandoned US20040241249A1 (en) | 2001-06-18 | 2002-06-07 | Krill and/or marine extracts for prevention and/or treatment of cardiovascular diseases arthritis, skin cancer diabetes, premenstrual syndrome and transdermal transport |
US11/640,235 Expired - Fee Related US8057825B2 (en) | 2001-06-18 | 2006-12-18 | Krill extracts for treatment of cardiovascular diseases |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US14/152,603 Abandoned US20140199414A1 (en) | 2001-06-18 | 2014-01-10 | Krill and/or marine extracts for prevention and/or treatment of cardiovascular diseases, arthritis, skin cancer, diabetes, premenstrual syndrome and transdermal transport |
US15/267,988 Abandoned US20170224742A1 (en) | 2001-06-18 | 2016-09-16 | Phospholipid-containing marine extracts for treatment of cardiovascular diseases |
Country Status (14)
Country | Link |
---|---|
US (5) | US20040241249A1 (en) |
EP (2) | EP1406641B1 (en) |
JP (4) | JP5135568B2 (en) |
CN (2) | CN1516592A (en) |
AT (2) | ATE554779T1 (en) |
CA (1) | CA2449898C (en) |
CY (1) | CY1108986T1 (en) |
DE (1) | DE60230759D1 (en) |
DK (2) | DK1406641T3 (en) |
ES (2) | ES2386841T3 (en) |
HK (2) | HK1126957A1 (en) |
NO (1) | NO332690B1 (en) |
PT (1) | PT1406641E (en) |
WO (1) | WO2002102394A2 (en) |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9171343B1 (en) | 2012-09-11 | 2015-10-27 | Aseko, Inc. | Means and method for improved glycemic control for diabetic patients |
US9233204B2 (en) | 2014-01-31 | 2016-01-12 | Aseko, Inc. | Insulin management |
US9486580B2 (en) | 2014-01-31 | 2016-11-08 | Aseko, Inc. | Insulin management |
US9629820B2 (en) | 2012-12-24 | 2017-04-25 | Qualitas Health, Ltd. | Eicosapentaenoic acid (EPA) formulations |
US9886556B2 (en) | 2015-08-20 | 2018-02-06 | Aseko, Inc. | Diabetes management therapy advisor |
US9892234B2 (en) | 2014-10-27 | 2018-02-13 | Aseko, Inc. | Subcutaneous outpatient management |
US9897565B1 (en) | 2012-09-11 | 2018-02-20 | Aseko, Inc. | System and method for optimizing insulin dosages for diabetic subjects |
US10052352B2 (en) | 2011-06-15 | 2018-08-21 | Stable Solutions Llc | Therapeutic application of parenteral krill oil |
US10123986B2 (en) | 2012-12-24 | 2018-11-13 | Qualitas Health, Ltd. | Eicosapentaenoic acid (EPA) formulations |
US11081226B2 (en) | 2014-10-27 | 2021-08-03 | Aseko, Inc. | Method and controller for administering recommended insulin dosages to a patient |
US11382980B2 (en) | 2013-10-08 | 2022-07-12 | Taiyo Kagaku Co., Ltd. | Oil/fat composition containing polyunsaturated fatty acid |
Families Citing this family (113)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2013205516B2 (en) * | 2001-07-27 | 2014-04-24 | Aker Biomarine Antarctic As | Natural marine source phospholipids comprising flavonoids, polyunsaturated fatty acids and their applications |
ES2288193T3 (en) | 2001-07-27 | 2008-01-01 | NEPTUNE TECHNOLOGIES & BIORESSOURCES INC. | NATURAL PHOSPHOLIPIDS OF MARINE ORIGIN CONTAINING FLAVONOIDS AND POLYINSATURATED PHOSPHOLIPIDS AND ITS USES. |
JP2003048831A (en) | 2001-08-02 | 2003-02-21 | Suntory Ltd | Composition having preventing and ameliorating action on symptom or disease caused by decrease in brain function |
NL1022443C2 (en) | 2003-01-20 | 2004-07-22 | Tno | Sphingolipids for improving the composition of the intestinal flora. |
WO2004064820A2 (en) * | 2003-01-20 | 2004-08-05 | Nederlandse Organisatie Voor Toegepast- Natuurwetenschappelijk Onderzoek Tno | Use of sphingolipids for reducing plasma cholesterol and triacylglycerol levels |
GB0314624D0 (en) | 2003-06-23 | 2003-07-30 | Advanced Bionutrition Europ Lt | Inflammatory disease treatment |
FR2859380B1 (en) * | 2003-09-05 | 2006-02-10 | Phythea | COMPOSITION FOR THE COSMETIC TREATMENT OF THE SKIN OF A HUMAN BEING |
US7935365B2 (en) | 2003-10-22 | 2011-05-03 | Enzymotec, Ltd. | Glycerophospholipids for the improvement of cognitive functions |
US20050130937A1 (en) | 2003-10-22 | 2005-06-16 | Enzymotec Ltd. | Lipids containing omega-3 and omega-6 fatty acids |
US8052992B2 (en) | 2003-10-22 | 2011-11-08 | Enzymotec Ltd. | Glycerophospholipids containing omega-3 and omega-6 fatty acids and their use in the treatment and improvement of cognitive functions |
CN103054901A (en) | 2003-10-28 | 2013-04-24 | 爱默蕾大学 | Dialysates and methods and systems related thereto |
JP4522075B2 (en) * | 2003-10-29 | 2010-08-11 | サントリーホールディングス株式会社 | Composition having an effect of preventing or ameliorating symptoms or diseases caused by aging of blood vessels |
WO2005075613A1 (en) * | 2004-02-06 | 2005-08-18 | Adrien Beaudoin | Method for preventing the oxidation of lipids in animal and vegetable oils and compositions produced by the method thereof |
ATE500752T1 (en) * | 2004-03-16 | 2011-03-15 | Tno | USE OF SPHINGOLIPIDS IN THE TREATMENT AND PREVENTION OF TYPE 2 DIABETES MELLITUS, INSULIN RESISTANCE AND METABOLISM SYNDROME |
JP4993852B2 (en) | 2004-09-17 | 2012-08-08 | サントリーホールディングス株式会社 | Composition having a preventive or ameliorating effect on symptoms or diseases accompanied by behavioral abnormalities caused by stress |
US7666447B2 (en) | 2004-10-08 | 2010-02-23 | Pharmanutrients | Compositions including Krill extracts and conjugated linoleic acid and methods of using same |
JP2008521888A (en) | 2004-11-30 | 2008-06-26 | ネーデルランドセ オルガニサティエ フォール トエゲパストナトールヴェテンシャッペリク オンデルゾエク ティエヌオー | Sphingolipids in the treatment and prevention of steatosis or hepatotoxicity and its sequelae |
JP2006016408A (en) * | 2005-06-23 | 2006-01-19 | Yamaha Motor Co Ltd | Agent for reducing neutral fat in blood |
JP5967855B2 (en) | 2005-06-30 | 2016-08-10 | サントリーホールディングス株式会社 | Composition having an activity of reducing daytime activity and / or depressive symptoms |
WO2007045407A2 (en) * | 2005-10-18 | 2007-04-26 | Artmed S.R.L. | Nutraceutical and pharmaceutical compositions containing choline as adiuvants for the prevention and treatment of retinopathies and glaucoma |
AR059012A1 (en) * | 2006-01-13 | 2008-03-05 | Aker Biomarine Asa | EXTRACTS DERIVED FROM KRILL |
WO2007091070A1 (en) * | 2006-02-07 | 2007-08-16 | Universitetet I Oslo | Omega 3 |
US20100048704A1 (en) * | 2006-07-14 | 2010-02-25 | Nattopharma Asa | Pharmaceutical and nutraceutical products comprising vitamin k2 |
KR20090077081A (en) * | 2006-11-03 | 2009-07-14 | 프로노바 바이오파마 노르지 에이에스 | Fatty acid alcohols |
EP3593797A1 (en) | 2006-12-28 | 2020-01-15 | Suntory Holdings Limited | Nerve-regenerating agent |
AU2014100740B4 (en) * | 2007-03-28 | 2014-11-20 | Aker Biomarine Antarctic As | Processes and products thereof |
AU2011213836B2 (en) * | 2007-03-28 | 2013-11-14 | Aker Biomarine Antarctic As | Bioeffective krill oil compositions |
EP2612672A1 (en) * | 2007-03-28 | 2013-07-10 | Aker BioMarine AS | Bioeffective krill oil compositions |
US8697138B2 (en) | 2007-03-28 | 2014-04-15 | Aker Biomarine As | Methods of using krill oil to treat risk factors for cardiovascular, metabolic, and inflammatory disorders |
AU2012244229B2 (en) * | 2007-08-29 | 2013-11-21 | Aker Biomarine Antarctic As | A new method for making krill meal |
US20100226977A1 (en) * | 2007-08-29 | 2010-09-09 | Aker Biomarine Asa | Low viscosity phospholipid compositions |
AU2014100741B4 (en) * | 2007-08-29 | 2014-09-11 | Aker Biomarine Antarctic As | Processes and products thereof |
US20090061067A1 (en) * | 2007-08-29 | 2009-03-05 | Aker Biomarine Asa | Method for making krill meal |
US8911888B2 (en) | 2007-12-16 | 2014-12-16 | HGST Netherlands B.V. | Three-dimensional magnetic memory with multi-layer data storage layers |
US8586104B2 (en) * | 2008-04-10 | 2013-11-19 | U.S. Nutraceuticals, LLC | Plant derived seed extract rich in essentially fatty acids derived from Salvia hispanica L. seed: composition of matter, manufacturing process and use |
BRPI0912749A2 (en) * | 2008-05-15 | 2017-05-23 | Pronova Biopharma Norge As | Krill oil process. |
US9239334B2 (en) * | 2008-09-08 | 2016-01-19 | President And Fellows Of Harvard College | Fatty acid C16: 1N7-palmitoleate a lipokine and biomarker for metabolic status |
US9814256B2 (en) | 2009-09-14 | 2017-11-14 | Rimfrost Technologies As | Method for processing crustaceans to produce low fluoride/low trimethyl amine products thereof |
EP3238551B1 (en) | 2008-09-12 | 2019-11-27 | Rimfrost Technologies AS | Process for reducing the fluoride content when producing proteinaceous concentrates from krill |
US8557297B2 (en) | 2008-09-12 | 2013-10-15 | Olympic Seafood, As | Method for processing crustaceans and products thereof |
KR101024491B1 (en) * | 2008-12-31 | 2011-03-31 | 인성실업(주) | The Method of Extracting Lipids from Marine Animals |
US8372812B2 (en) | 2009-02-26 | 2013-02-12 | Aker Biomarine Asa | Phospholipid and protein tablets |
JP2010241710A (en) * | 2009-04-02 | 2010-10-28 | Japan Health Science Foundation | Ace2 activity inducer |
US9925162B2 (en) | 2009-04-09 | 2018-03-27 | The Regents Of The University Of Colorado | Methods and compositions for inducing physiological hypertrophy |
WO2010136900A2 (en) | 2009-05-28 | 2010-12-02 | Aker Biomarine Asa | Methods of using krill oil to treat risk factors for metabolic, cardiovascular, and inflammatory disorders |
US8481072B2 (en) * | 2009-07-23 | 2013-07-09 | U.S. Nutraceuticals, LLC | Composition and method to alleviate joint pain |
US9913810B2 (en) | 2009-07-23 | 2018-03-13 | U.S. Nutraceuticals, LLC | Composition and method to alleviate joint pain using phospholipids and astaxanthin |
US9238043B2 (en) | 2009-07-23 | 2016-01-19 | U.S. Nutraceuticals, LLC | Composition and method to alleviate joint pain using algae based oils |
US9402857B2 (en) | 2009-07-23 | 2016-08-02 | U.S. Nutraceuticals, LLC | Composition and method to alleviate joint pain using low molecular weight hyaluronic acid and astaxanthin |
US9216164B2 (en) | 2009-07-23 | 2015-12-22 | U.S. Nutraceuticals, LLC | Composition and method to alleviate joint pain using a mixture of fish oil and fish oil derived, choline based, phospholipid bound fatty acid mixture including polyunsaturated EPA and DHA |
US8557275B2 (en) | 2009-07-23 | 2013-10-15 | U.S. Nutraceuticals, LLC | Composition and method to alleviate joint pain using a mixture of fish oil and fish oil derived, choline based, phospholipid bound fatty acid mixture including polyunsaturated EPA and DHA |
US9399047B2 (en) | 2009-07-23 | 2016-07-26 | U.S. Nutraceuticals, LLC | Composition and method to alleviate joint pain using phospholipids and roe extract |
US20130295171A1 (en) | 2009-07-23 | 2013-11-07 | U.S NUTRACEUTICALS, LLC d/b/a Valensa International | Krill oil and reacted astaxanthin composition and associated method |
TWI494110B (en) | 2009-10-29 | 2015-08-01 | Acasti Pharma Inc | Concentrated therapeutic phospholipid compositions |
AU2009354456B2 (en) | 2009-10-30 | 2014-05-08 | Tharos Ltd | Solvent-free process for obtaining phospholipids and neutral enriched krill oils |
CA2780593A1 (en) * | 2009-11-11 | 2011-05-19 | Dynasep Inc. | Energy efficient acetone drying method |
US20110117207A1 (en) * | 2009-11-17 | 2011-05-19 | U.S. Nutraceuticals, LLC d/b/a Valensa International State of Incorporation: | Use of eggshell membrane formulations to alleviate joint pain |
US9763897B2 (en) | 2010-04-30 | 2017-09-19 | U.S. Nutraceuticals, LLC | Therapeutic astaxanthin and phospholipid composition and associated method |
WO2011137160A2 (en) | 2010-04-30 | 2011-11-03 | U.S. Nutraceuticals, Llc D/B/A Valensa International | Composition and method to improve blood lipid profiles and optionally reduce low density lipoprotein (ldl) per-oxidation in humans |
US8663704B2 (en) | 2010-04-30 | 2014-03-04 | U.S. Nutraceuticals, LLC | Composition and method to improve blood lipid profiles and optionally reduce low density lipoprotein (LDL) per-oxidation in humans |
WO2012029898A1 (en) * | 2010-09-01 | 2012-03-08 | 日本水産株式会社 | Mitigating agent for alcohol-induced problems |
KR20120051458A (en) * | 2010-11-12 | 2012-05-22 | 주식회사 두산 | Method of preparing a composition including astaxanthin and dha- and/or epa-conjugated phosphatidylserine using krill-derived lecithin, and a composition prepared by the method |
CN102485899A (en) * | 2010-12-03 | 2012-06-06 | 张永志 | Method for preparing high quality Antarctic krill oil rich in phosphatidylserine containing polyunsaturated double-bonded fatty acyl |
CN102041166B (en) * | 2011-01-20 | 2012-05-23 | 山东师范大学 | Method for extracting krill oil with high phosphatide content from Antarctic krills |
WO2012103685A1 (en) * | 2011-02-01 | 2012-08-09 | Nippon Suisan Kaisha, Ltd. | Sexual function improving agent |
KR101045258B1 (en) * | 2011-02-11 | 2011-06-30 | 대덕에프알디(주) | Krill oil and method for manufacturing the same |
US20120231087A1 (en) * | 2011-03-07 | 2012-09-13 | Olympic Seafood | Compositions And Methods for Nutritional Supplementation |
CA2838312C (en) * | 2011-06-15 | 2019-01-08 | Stable Solutions Llc | Therapeutic application of parenteral krill oil |
WO2012178118A1 (en) * | 2011-06-24 | 2012-12-27 | Epitogenesis Inc. | Pharmaceutical compositions, comprising a combination of select carriers, vitamins, tannins and flavonoids as antigen-specific immuno-modulators |
JP6067558B2 (en) * | 2011-06-29 | 2017-01-25 | 日本水産株式会社 | How to reduce fear memory |
WO2013033618A1 (en) | 2011-09-02 | 2013-03-07 | Arctic Nutrition As | Lipid compositions with high dha content |
UA114615C2 (en) | 2012-01-06 | 2017-07-10 | Омтера Фармасьютікалз, Інк. | Dpa-enriched compositions of omega-3 polyunsaturated fatty acids in free acid form |
CN102559371B (en) * | 2012-02-21 | 2013-09-18 | 山东师范大学 | Method for preparing phosphatidylserine-enriched krill oil |
WO2013169797A1 (en) | 2012-05-07 | 2013-11-14 | Omthera Pharmaceuticals, Inc. | Compositions of statins and omega-3 fatty acids |
EP2894242B1 (en) | 2012-09-07 | 2020-01-15 | Japan Science and Technology Agency | Guest-compound-enveloping polymer-metal-complex crystal, method for producing same, method for preparing crystal structure analysis sample, and method for determining molecular structure of organic compound |
CN102899162B (en) * | 2012-10-25 | 2013-12-04 | 山东师范大学 | Technology for preparing euphausia superba oil in low fluorine content |
CN102899163B (en) * | 2012-10-25 | 2015-03-25 | 山东师范大学 | Process for extracting fluoride-free shrimp oil from antarctic krill |
CN102925284B (en) * | 2012-10-25 | 2014-01-08 | 山东师范大学 | Technique for extracting low-cholesterol-content krill oil from Antarctic krill |
CN102911786B (en) * | 2012-10-25 | 2014-01-08 | 山东师范大学 | Technology for extracting shrimp oil with low fluoride from euphausia superba |
CN102899164B (en) * | 2012-10-25 | 2015-12-02 | 山东师范大学 | A kind of technique preparing fluoride-free euphausia superba oil |
WO2014184655A1 (en) | 2013-02-07 | 2014-11-20 | Olympic Seafood As | Methods for using crustacean phospholipid-peptide-protein complexes |
AU2014203179C1 (en) | 2013-06-14 | 2017-05-04 | Aker Biomarine Antarctic As | Lipid extraction processes |
JP6393501B2 (en) * | 2014-04-03 | 2018-09-19 | 太陽化学株式会社 | Flavor improving agent for polyunsaturated fatty acids |
JP2015096488A (en) * | 2013-10-10 | 2015-05-21 | 康二 嘉島 | Method for inhibiting production of advanced glycation endproducts |
GB201400431D0 (en) | 2014-01-10 | 2014-02-26 | Aker Biomarine As | Phospholipid compositions and their preparation |
WO2015142700A1 (en) | 2014-03-18 | 2015-09-24 | U.S. Nutraceuticals, Llc D/B/A Valensa International | Composition and method to alleviate joint pain using phospholipids and astaxanthin |
WO2015142702A1 (en) | 2014-03-18 | 2015-09-24 | U.S. Nutraceuticals, Llc D/B/A Valensa International | Composition and method to alleviate joint pain using phospholipids and roe extract |
WO2015142707A1 (en) | 2014-03-18 | 2015-09-24 | U.S. Nutraceuticals, Llc D/B/A Valensa International | Composition and method to alleviate joint pain using low molecular weight hyaluronic acid and astaxanthin |
WO2015143001A1 (en) | 2014-03-19 | 2015-09-24 | U.S. Nutraceuticals, Llc D/B/A Valensa International | Therapeutic astaxanthin and phospholipid composition and associated method |
PL3137070T3 (en) * | 2014-04-30 | 2020-07-13 | Aker Biomarine Antarctic As | Krill oil preparations and their uses |
JP6299689B2 (en) * | 2014-07-24 | 2018-03-28 | 三生医薬株式会社 | Bioabsorption promoter-containing composition |
WO2016128830A1 (en) | 2015-02-11 | 2016-08-18 | Aker Biomarine Antarctic As | Lipid extraction processes |
KR102310765B1 (en) | 2015-02-11 | 2021-10-12 | 에이커 바이오마린 앤탁틱 에이에스 | Lipid compositions |
US10328105B2 (en) | 2015-05-27 | 2019-06-25 | Rimfrost Technologies As | Flowable concentrated phospholipid krill oil composition |
CN105341184A (en) * | 2015-10-14 | 2016-02-24 | 中国农业科学院油料作物研究所 | Functional fat composition having effects of preventing cardiovascular and cerebrovascular diseases and diabetes risk factors |
AU2016374155A1 (en) * | 2015-12-14 | 2018-04-19 | Société des Produits Nestlé S.A. | Nutritional composition and infant formula for promoting myelination of the brain |
DE202015106826U1 (en) * | 2015-12-15 | 2016-02-03 | Wolfgang Braun | Composition for the treatment of premenstrual syndrome |
JP2019520384A (en) | 2016-06-29 | 2019-07-18 | マーシャヴェール ファーマシューティカルズ リミテッド ライアビリティ カンパニー | Phospholipid composition |
BR112019008739A2 (en) * | 2016-11-04 | 2019-07-09 | Immd Sp Zo O | intelligent distribution of ingested and absorbed molecules |
JP7309136B2 (en) * | 2017-04-14 | 2023-07-18 | 学校法人帝京大学 | HDL function improving agent and HDL function improving food composition |
AU2018392997A1 (en) | 2017-12-22 | 2019-08-15 | Pharmalink International Limited | Lipid combinations |
DE202018105987U1 (en) | 2018-05-24 | 2018-12-19 | U.S. Nutraceuticals, Llc D/B/A Valensa International | A composition for relieving joint pain using hyaluronic acid and egg shell membrane components |
JP2019210239A (en) * | 2018-06-02 | 2019-12-12 | 学校法人 岩手医科大学 | Pharmacological use of 8-hydroxyeicosapentaenoic acid |
DE202018105422U1 (en) | 2018-06-21 | 2018-10-19 | U.S. Nutraceuticals, Llc D/B/A Valensa International | A composition for relieving joint pain using hyaluronic acid and egg shell membrane components |
BR112021004809A2 (en) * | 2018-06-28 | 2021-06-22 | Marizyme Biotech | pharmaceutical compositions and methods for the treatment of thrombosis and application by medical devices |
US20220265291A9 (en) * | 2018-06-28 | 2022-08-25 | Marizyme, Inc. | Pharmaceutical compositions and methods for the treatment of thrombosis and delivery by medical devices |
KR102085506B1 (en) * | 2018-07-25 | 2020-03-05 | 이경희 | Composition for hair colorants having anti-irritation effect |
WO2021138742A1 (en) * | 2020-01-10 | 2021-07-15 | Acasti Pharma Inc. | Composition that promote pro-resolving mediators |
IT202000003964A1 (en) * | 2020-02-26 | 2021-08-26 | Umberto Cornelli | PHARMACEUTICAL COMPOSITION FOR USE IN THE TREATMENT OF DYSMENORREA AND / OR PREMESTRUAL SYNDROME |
KR102451162B1 (en) * | 2020-09-07 | 2022-10-06 | (주)에스디생명공학 | Composition for Anti-Inflammatory, Anti-Wrinkling, Moisturizing, and Skin Cell Regeneration Property Comprising Enzyme Treated Extract of Krill Oil as Active Ingredient |
CN112315984B (en) * | 2020-09-09 | 2023-02-14 | 山东省科学院生物研究所 | Application of marine-derived phospholipid in promoting angiogenesis |
CN112494499B (en) * | 2020-12-14 | 2022-01-28 | 山东省科学院生物研究所 | Application of marine phospholipid as effective component in preparing medicine for preventing and/or treating heart diseases |
AU2022224703A1 (en) * | 2021-09-28 | 2023-04-13 | Samuel L. Shepherd | Tetraterpenes for use in cancer therapy |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS54119017A (en) * | 1978-03-06 | 1979-09-14 | Taiyo Fishery Co Ltd | Protective agent for hypertensive disease and production |
JPS60153779A (en) | 1984-01-24 | 1985-08-13 | Hohnen Oil Co Ltd | Nutrient-assisting food |
JPS6130527A (en) * | 1984-07-20 | 1986-02-12 | Kao Corp | Thrombolytic agent |
JPS61118318A (en) * | 1984-11-12 | 1986-06-05 | Pola Chem Ind Inc | Composition having improving action on serum lipid |
JPS63295698A (en) * | 1987-05-27 | 1988-12-02 | Showa Denko Kk | Separation and purification of alpha-linolenic acid |
US4843095A (en) * | 1987-08-07 | 1989-06-27 | Century Laboratories, Inc. | Free fatty acids for treatment or propyhlaxis of rheumatoid arthritis arthritis |
JPH01137952A (en) * | 1987-11-20 | 1989-05-30 | Nippon Suisan Kaisha Ltd | Health food |
JPH02167055A (en) * | 1988-12-19 | 1990-06-27 | Seiji Nojima | Functional fish-paste product |
JPH03123470A (en) * | 1989-10-05 | 1991-05-27 | Taiyo Fishery Co Ltd | Method for enhancing angiotensin conversion enzyme inhibiting activity in krill |
JP2558050B2 (en) * | 1993-02-18 | 1996-11-27 | イセ食品株式会社 | Chicken feed |
JPH08219A (en) * | 1994-06-15 | 1996-01-09 | Nippon Synthetic Chem Ind Co Ltd:The | Oil composition having improved function |
JP2666179B2 (en) * | 1994-08-10 | 1997-10-22 | 財団法人韓國食品開發研究院 | Antihypertensive health food composition |
JPH09124470A (en) * | 1995-10-26 | 1997-05-13 | Suntory Ltd | Antistress composition |
JP3664814B2 (en) * | 1996-06-26 | 2005-06-29 | 旭化成ケミカルズ株式会社 | Blood lipid improving agent and food additive |
JP3118556B2 (en) * | 1996-09-27 | 2000-12-18 | 中央水産研究所長 | Fish meat emulsified surimi containing high concentration of fats and oils by fish meat water-soluble protein and method for producing the same |
CA2251265A1 (en) * | 1998-10-21 | 2000-04-21 | Universite De Sherbrooke | Process for lipid extraction of aquatic animal tissues producing a dehydrated residue |
-
2002
- 2002-06-07 PT PT02734945T patent/PT1406641E/en unknown
- 2002-06-07 JP JP2003504980A patent/JP5135568B2/en not_active Expired - Lifetime
- 2002-06-07 CN CNA028121813A patent/CN1516592A/en active Pending
- 2002-06-07 US US10/481,040 patent/US20040241249A1/en not_active Abandoned
- 2002-06-07 AT AT08164524T patent/ATE554779T1/en active
- 2002-06-07 ES ES08164524T patent/ES2386841T3/en not_active Expired - Lifetime
- 2002-06-07 EP EP02734945A patent/EP1406641B1/en not_active Expired - Lifetime
- 2002-06-07 EP EP08164524A patent/EP1997498B1/en not_active Revoked
- 2002-06-07 ES ES02734945T patent/ES2321066T3/en not_active Expired - Lifetime
- 2002-06-07 DE DE60230759T patent/DE60230759D1/en not_active Expired - Lifetime
- 2002-06-07 DK DK02734945T patent/DK1406641T3/en active
- 2002-06-07 AT AT02734945T patent/ATE419858T1/en active
- 2002-06-07 CN CN201110219831.4A patent/CN102319266B/en not_active Expired - Lifetime
- 2002-06-07 CA CA2449898A patent/CA2449898C/en not_active Expired - Lifetime
- 2002-06-07 WO PCT/CA2002/000843 patent/WO2002102394A2/en active Application Filing
- 2002-06-07 DK DK08164524.4T patent/DK1997498T3/en active
-
2003
- 2003-12-16 NO NO20035618A patent/NO332690B1/en not_active IP Right Cessation
-
2006
- 2006-12-18 US US11/640,235 patent/US8057825B2/en not_active Expired - Fee Related
-
2009
- 2009-04-06 CY CY20091100401T patent/CY1108986T1/en unknown
- 2009-05-29 HK HK09104847.8A patent/HK1126957A1/en not_active IP Right Cessation
- 2009-12-04 JP JP2009276479A patent/JP5228185B2/en not_active Expired - Lifetime
- 2009-12-04 JP JP2009276478A patent/JP2010090140A/en active Pending
-
2011
- 2011-08-24 US US13/216,694 patent/US20110305771A1/en not_active Abandoned
-
2012
- 2012-07-04 HK HK12106558.7A patent/HK1165729A1/en not_active IP Right Cessation
-
2013
- 2013-01-08 JP JP2013001424A patent/JP2013079278A/en active Pending
-
2014
- 2014-01-10 US US14/152,603 patent/US20140199414A1/en not_active Abandoned
-
2016
- 2016-09-16 US US15/267,988 patent/US20170224742A1/en not_active Abandoned
Cited By (48)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10052352B2 (en) | 2011-06-15 | 2018-08-21 | Stable Solutions Llc | Therapeutic application of parenteral krill oil |
US9811638B2 (en) | 2012-09-11 | 2017-11-07 | Aseko, Inc. | Means and method for improved glycemic control for diabetic patients |
US9773096B2 (en) | 2012-09-11 | 2017-09-26 | Aseko, Inc. | Means and method for improved glycemic control for diabetic patients |
US9897565B1 (en) | 2012-09-11 | 2018-02-20 | Aseko, Inc. | System and method for optimizing insulin dosages for diabetic subjects |
US10102922B2 (en) | 2012-09-11 | 2018-10-16 | Aseko, Inc. | Means and method for improved glycemic control for diabetic patients |
US11131643B2 (en) | 2012-09-11 | 2021-09-28 | Aseko, Inc. | Method and system for optimizing insulin dosages for diabetic subjects |
US9171343B1 (en) | 2012-09-11 | 2015-10-27 | Aseko, Inc. | Means and method for improved glycemic control for diabetic patients |
US11733196B2 (en) | 2012-09-11 | 2023-08-22 | Aseko, Inc. | System and method for optimizing insulin dosages for diabetic subjects |
US9965596B2 (en) | 2012-09-11 | 2018-05-08 | Aseko, Inc. | Means and method for improved glycemic control for diabetic patients |
US10629294B2 (en) | 2012-09-11 | 2020-04-21 | Aseko, Inc. | Means and method for improved glycemic control for diabetic patients |
US10410740B2 (en) | 2012-09-11 | 2019-09-10 | Aseko, Inc. | Means and method for improved glycemic control for diabetic patients |
US9483619B2 (en) | 2012-09-11 | 2016-11-01 | Aseko, Inc. | Means and method for improved glycemic control for diabetic patients |
US9629820B2 (en) | 2012-12-24 | 2017-04-25 | Qualitas Health, Ltd. | Eicosapentaenoic acid (EPA) formulations |
US10123986B2 (en) | 2012-12-24 | 2018-11-13 | Qualitas Health, Ltd. | Eicosapentaenoic acid (EPA) formulations |
US10039734B2 (en) | 2012-12-24 | 2018-08-07 | Qualitas Health, Ltd. | Eicosapentaenoic acid (EPA) formulations |
US11382980B2 (en) | 2013-10-08 | 2022-07-12 | Taiyo Kagaku Co., Ltd. | Oil/fat composition containing polyunsaturated fatty acid |
US9892235B2 (en) | 2014-01-31 | 2018-02-13 | Aseko, Inc. | Insulin management |
US10535426B2 (en) | 2014-01-31 | 2020-01-14 | Aseko, Inc. | Insulin management |
US9233204B2 (en) | 2014-01-31 | 2016-01-12 | Aseko, Inc. | Insulin management |
US9504789B2 (en) | 2014-01-31 | 2016-11-29 | Aseko, Inc. | Insulin management |
US9898585B2 (en) | 2014-01-31 | 2018-02-20 | Aseko, Inc. | Method and system for insulin management |
US10255992B2 (en) | 2014-01-31 | 2019-04-09 | Aseko, Inc. | Insulin management |
US11804300B2 (en) | 2014-01-31 | 2023-10-31 | Aseko, Inc. | Insulin management |
US11783945B2 (en) | 2014-01-31 | 2023-10-10 | Aseko, Inc. | Method and system for insulin infusion rate management |
US11783946B2 (en) | 2014-01-31 | 2023-10-10 | Aseko, Inc. | Method and system for insulin bolus management |
US11857314B2 (en) | 2014-01-31 | 2024-01-02 | Aseko, Inc. | Insulin management |
US10453568B2 (en) | 2014-01-31 | 2019-10-22 | Aseko, Inc. | Method for managing administration of insulin |
US9965595B2 (en) | 2014-01-31 | 2018-05-08 | Aseko, Inc. | Insulin management |
US11490837B2 (en) | 2014-01-31 | 2022-11-08 | Aseko, Inc. | Insulin management |
US10811133B2 (en) | 2014-01-31 | 2020-10-20 | Aseko, Inc. | System for administering insulin boluses to a patient |
US9710611B2 (en) | 2014-01-31 | 2017-07-18 | Aseko, Inc. | Insulin management |
US11081233B2 (en) | 2014-01-31 | 2021-08-03 | Aseko, Inc. | Insulin management |
US9604002B2 (en) | 2014-01-31 | 2017-03-28 | Aseko, Inc. | Insulin management |
US11158424B2 (en) | 2014-01-31 | 2021-10-26 | Aseko, Inc. | Insulin management |
US11621074B2 (en) | 2014-01-31 | 2023-04-04 | Aseko, Inc. | Insulin management |
US11311213B2 (en) | 2014-01-31 | 2022-04-26 | Aseko, Inc. | Insulin management |
US9486580B2 (en) | 2014-01-31 | 2016-11-08 | Aseko, Inc. | Insulin management |
US11468987B2 (en) | 2014-01-31 | 2022-10-11 | Aseko, Inc. | Insulin management |
US9892234B2 (en) | 2014-10-27 | 2018-02-13 | Aseko, Inc. | Subcutaneous outpatient management |
US11678800B2 (en) | 2014-10-27 | 2023-06-20 | Aseko, Inc. | Subcutaneous outpatient management |
US11694785B2 (en) | 2014-10-27 | 2023-07-04 | Aseko, Inc. | Method and dosing controller for subcutaneous outpatient management |
US11081226B2 (en) | 2014-10-27 | 2021-08-03 | Aseko, Inc. | Method and controller for administering recommended insulin dosages to a patient |
US10403397B2 (en) | 2014-10-27 | 2019-09-03 | Aseko, Inc. | Subcutaneous outpatient management |
US10128002B2 (en) | 2014-10-27 | 2018-11-13 | Aseko, Inc. | Subcutaneous outpatient management |
US11574742B2 (en) | 2015-08-20 | 2023-02-07 | Aseko, Inc. | Diabetes management therapy advisor |
US11200988B2 (en) | 2015-08-20 | 2021-12-14 | Aseko, Inc. | Diabetes management therapy advisor |
US10380328B2 (en) | 2015-08-20 | 2019-08-13 | Aseko, Inc. | Diabetes management therapy advisor |
US9886556B2 (en) | 2015-08-20 | 2018-02-06 | Aseko, Inc. | Diabetes management therapy advisor |
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US8057825B2 (en) | Krill extracts for treatment of cardiovascular diseases | |
JP3008213B2 (en) | Pharmaceutical composition | |
US7666447B2 (en) | Compositions including Krill extracts and conjugated linoleic acid and methods of using same | |
US20030166614A1 (en) | Method for reducing cholesterol and triglycerides | |
EP1871399B1 (en) | Use of lecithin as a medicament for treating psoriasis | |
RU2366412C2 (en) | Compositions and treatment methods for hyperproliferative skin conditions | |
EP3461479B1 (en) | Nutraceutical and pharmaceutical compositions, and uses thereof for preserving cognitive functions | |
US20040091506A1 (en) | Topical antifungal treatment | |
US11039997B2 (en) | Cosmetic, dermatic, protective compositions comprising phospholipids, lecithins with peptides and at least one acetylating compound | |
US10517838B2 (en) | Compositions against alkyl-acyl GPC, the derivatives and products thereof | |
US20060009486A1 (en) | Composition and method for treatment and prevention of coronary artery disease | |
US20050137257A1 (en) | Treating eczema and/or psoriasis | |
Lieberman | Natural Interventions for Treating Psoriasis | |
RU2351350C1 (en) | Set "predstar", its application and method of prevention of premature organism aging |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AS | Assignment |
Owner name: NEPTUNE TECHNOLOGIES & BIORESSOURCES, INC., CANADA Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:SAMPALIS, TINA;REEL/FRAME:028908/0846 Effective date: 20120905 |
|
STCB | Information on status: application discontinuation |
Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION |
|
AS | Assignment |
Owner name: BANK AND CLIENTS PLC, ENGLAND Free format text: MORTGAGE;ASSIGNOR:NEPTUNE TECHNOLOGIES & BIORESSOURCES INC.;REEL/FRAME:038463/0304 Effective date: 20160418 |
|
AS | Assignment |
Owner name: NEPTUNE TECHNOLOGIES & BIORESSOURCES INC., CANADA Free format text: RELEASE BY SECURED PARTY;ASSIGNOR:BANK AND CLIENTS PLC;REEL/FRAME:043389/0273 Effective date: 20170802 |
|
AS | Assignment |
Owner name: AKER BIOMARINE ANTARCTIC AS, NORWAY Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:NEPTUNE TECHNOLOGIES & BIORESSOURCES, INC.;REEL/FRAME:043525/0925 Effective date: 20170905 |