US20090325283A1 - Cell deposition system - Google Patents

Cell deposition system Download PDF

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Publication number
US20090325283A1
US20090325283A1 US12/375,784 US37578407A US2009325283A1 US 20090325283 A1 US20090325283 A1 US 20090325283A1 US 37578407 A US37578407 A US 37578407A US 2009325283 A1 US2009325283 A1 US 2009325283A1
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US
United States
Prior art keywords
chamber
plate
cells
depositing
fixing agent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US12/375,784
Inventor
Eric Roger Louis Peltier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novacyt SA
Original Assignee
Novacyt SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novacyt SA filed Critical Novacyt SA
Assigned to NOVACYT reassignment NOVACYT ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: PELTIER, ERIC ROGER, LEWIS
Publication of US20090325283A1 publication Critical patent/US20090325283A1/en
Abandoned legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0621Control of the sequence of chambers filled or emptied
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0647Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668Trapping microscopic beads
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/069Absorbents; Gels to retain a fluid
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/06Valves, specific forms thereof
    • B01L2400/0633Valves, specific forms thereof with moving parts
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G01N2001/2826Collecting by adsorption or absorption
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G01N2001/2846Cytocentrifuge method

Definitions

  • the present invention relates to a system for depositing cells on an analysis plate.
  • cell samples are taken and are placed in suspension so that they can be analysed.
  • these samples can be taken using specific brushes, needles or any other appropriate means and are then introduced into a bottle containing a cell fixing agent so that the cells which have been removed are fixed by the fixing agent and form a suspension therewith.
  • the cells must then be deposited on an analysis plate.
  • centrifuging means and filtering means have been used to obtain this cell deposit on the plate.
  • the deposit of the cells can also be obtained by means of a simple decantation operation.
  • the cell suspension comprising the cell fixing agent and the cells is poured into a receiving chamber which is positioned above the analysis plate and whose base is open and extends opposite a cell depositing zone of the analysis plate.
  • the cells are deposited progressively on the plate, then the fixing agent is withdrawn from the chamber.
  • document FR-A-2 792 333 proposes a system for depositing cells on an analysis plate, the cells being contained in a cell suspension which comprises a cell fixing agent and the cells, the suspension being poured into a receiving chamber which is positioned above the analysis plate and whose base is open and extends opposite a cell depositing zone of the analysis plate.
  • the base of the chamber is in fluid communication with a material for absorbing the fixing agent in order to progressively absorb it and to allow a homogeneous deposit of the cells on the cell depositing zone of the analysis plate.
  • the object of the invention is therefore to overcome these problems.
  • the invention relates to a system for depositing cells on an analysis plate, the cells being contained in a cell suspension comprising a fixing agent and the cells, comprising, for receiving the suspension, a chamber which is positioned above the analysis plate and whose base is open and extends opposite a cell depositing zone of the plate and a material for absorbing the fixing agent placed around the chamber and the depositing zone of the plate, characterised in that the chamber is mounted so as to be able to be moved between a first position in abutment against the analysis plate in order to allow the cells to be deposited on the depositing zone of the plate by means of decantation and a second position remote from this analysis plate in order to place the chamber in a fluid relationship with the absorption material in order to allow this material to absorb the fixing agent.
  • the system comprises one or more of the following features:
  • FIG. 1 is a schematic section of a depositing system according to the invention, in which the chamber is in its first position;
  • the analysis plate is generally designated 1 and is formed by any appropriate plate which is already known from the prior art.
  • This plate is received in a corresponding recess 2 of a component 3 which forms a base and which will be described in greater detail below.
  • a cell suspension which is generally designated 4 is poured into a receiving chamber 5 which is placed above the analysis plate and whose base is open and extends opposite a cell depositing zone of the analysis plate.
  • This receiving chamber 5 is, for example, placed in a support component 6 which will be described in greater detail below and which extends opposite the component which forms a base 3 .
  • the depositing system also comprises a material for absorbing the cell fixing agent.
  • This absorption material is generally designated 7 in these Figures and is, for example, in the form a sheet of absorbent paper which has the thickness of the liquid deposit (approximately 0.5 mm) and which is provided with a hole 8 which is capable of extending opposite the cell depositing zone of the analysis plate.
  • the absorption material extends around the cell depositing zone of the plate and the receiving chamber 5 and is therefore positioned between the components which form a base 3 and support components 6 around this chamber 5 for receiving the suspension.
  • the lower edge of the chamber 5 can be provided, for example, with a sealing joint 9 such as, for example, an O-ring and the chamber can be moved relative to this plate, as illustrated in FIGS. 1 and 2 using means for controlling the movement thereof, generally designated 10 .
  • a sealing joint 9 such as, for example, an O-ring and the chamber can be moved relative to this plate, as illustrated in FIGS. 1 and 2 using means for controlling the movement thereof, generally designated 10 .
  • control means may have any appropriate structure.
  • this chamber allows this chamber to be moved between the position illustrated in FIG. 1 , in which the chamber and more particularly the base thereof and the sealing joint are in abutment against the analysis plate, thus preventing the absorption material from absorbing the cell fixing agent of the suspension contained in the chamber.
  • the chamber is held in this position for a predetermined period of time, for example, of approximately 30 minutes in order to allow a homogeneous deposit by cells being decanted on the analysis plate, as illustrated.
  • control means bring about a movement of the chamber from the position illustrated in FIG. 1 to the position illustrated in FIG. 2 , in which this chamber is remote from the analysis plate, thus placing the fixing agent of the cell suspension and the absorption material in a fluid relationship.
  • Tests have shown that after a period of time of 30 minutes, the cells are already in the correct position and it is possible to initiate the absorption of the fixing agent in order to save time during the depositing operation of the cells on the analysis plate.
  • this structure allows the cells to be deposited in a rapid and homogeneous manner on the analysis plate, in particular in the form of a single layer, which facilitates their subsequent analysis, and that this structure is particularly well suited to the analysis of cytological suspensions.

Abstract

A system for depositing cells on an analysis plate, the cells being contained in a cell suspension including a fixing agent and the cells, included, for receiving the suspension, a chamber (5) which is positioned above the analysis plate and whose base is open and extends opposite a cell depositing zone of the plate and a material for absorbing the fixing agent placed around the chamber and the depositing zone of the plate. The chamber is mounted so as to be able to be moved between a first position in abutment against the analysis plate in order to allow the cells to be deposited on the depositing zone of the plate via decantation and a second position remote from this analysis plate in order to place the chamber in a fluid relationship with the absorption material in order to allow this material to absorb the fixing agent.

Description

  • The present invention relates to a system for depositing cells on an analysis plate.
  • In order to screen or diagnose lesions, cell samples are taken and are placed in suspension so that they can be analysed.
  • Generally, these samples can be taken using specific brushes, needles or any other appropriate means and are then introduced into a bottle containing a cell fixing agent so that the cells which have been removed are fixed by the fixing agent and form a suspension therewith.
  • The cells must then be deposited on an analysis plate.
  • Various deposition methods and systems for this type of application are already known from the prior art.
  • In this manner, for example, centrifuging means and filtering means have been used to obtain this cell deposit on the plate.
  • However, such means are relatively complex, bulky, costly and are not very easy to use.
  • The deposit of the cells can also be obtained by means of a simple decantation operation.
  • In this instance, the cell suspension comprising the cell fixing agent and the cells is poured into a receiving chamber which is positioned above the analysis plate and whose base is open and extends opposite a cell depositing zone of the analysis plate.
  • The cells are deposited progressively on the plate, then the fixing agent is withdrawn from the chamber.
  • However, it will be appreciated that this operation which involves removing the fixing agent without moving the cells is very long and complex.
  • In order to overcome these problems, document FR-A-2 792 333 proposes a system for depositing cells on an analysis plate, the cells being contained in a cell suspension which comprises a cell fixing agent and the cells, the suspension being poured into a receiving chamber which is positioned above the analysis plate and whose base is open and extends opposite a cell depositing zone of the analysis plate. According to this system, the base of the chamber is in fluid communication with a material for absorbing the fixing agent in order to progressively absorb it and to allow a homogeneous deposit of the cells on the cell depositing zone of the analysis plate.
  • When used, however, such a system also had a given number of disadvantages, in particular with respect to the time required for the absorption of the fixing agent and therefore the deposit of the cells on the analysis plate.
  • The object of the invention is therefore to overcome these problems.
  • To this end, the invention relates to a system for depositing cells on an analysis plate, the cells being contained in a cell suspension comprising a fixing agent and the cells, comprising, for receiving the suspension, a chamber which is positioned above the analysis plate and whose base is open and extends opposite a cell depositing zone of the plate and a material for absorbing the fixing agent placed around the chamber and the depositing zone of the plate, characterised in that the chamber is mounted so as to be able to be moved between a first position in abutment against the analysis plate in order to allow the cells to be deposited on the depositing zone of the plate by means of decantation and a second position remote from this analysis plate in order to place the chamber in a fluid relationship with the absorption material in order to allow this material to absorb the fixing agent.
  • According to specific embodiments, the system comprises one or more of the following features:
      • the base of the chamber is provided with sealing means;
      • the chamber is connected to means for moving the chamber between its two positions at the end of a predetermined period of time;
      • the predetermined period of time is approximately 30 minutes; and
      • the absorption material is in the form of a sheet of absorbent paper which is placed around the depositing zone of the plate and the chamber.
  • The invention will be better understood from a reading of the following description, given purely by way of example and with reference to the appended drawings, in which:
  • FIG. 1 is a schematic section of a depositing system according to the invention, in which the chamber is in its first position; and
      • FIG. 2 is a similar view, in which the chamber is in its second position.
  • These Figures illustrate a system for depositing cells on an analysis plate.
  • The analysis plate is generally designated 1 and is formed by any appropriate plate which is already known from the prior art.
  • This plate is received in a corresponding recess 2 of a component 3 which forms a base and which will be described in greater detail below.
  • A cell suspension which is generally designated 4 is poured into a receiving chamber 5 which is placed above the analysis plate and whose base is open and extends opposite a cell depositing zone of the analysis plate.
  • This receiving chamber 5 is, for example, placed in a support component 6 which will be described in greater detail below and which extends opposite the component which forms a base 3.
  • The depositing system also comprises a material for absorbing the cell fixing agent.
  • This absorption material is generally designated 7 in these Figures and is, for example, in the form a sheet of absorbent paper which has the thickness of the liquid deposit (approximately 0.5 mm) and which is provided with a hole 8 which is capable of extending opposite the cell depositing zone of the analysis plate.
  • The absorption material extends around the cell depositing zone of the plate and the receiving chamber 5 and is therefore positioned between the components which form a base 3 and support components 6 around this chamber 5 for receiving the suspension.
  • These components which form a base and support can be fixed and locked to each other, for example, using fixing means and locking means which allow the sheet of absorption material to be held in position by means of clamping therebetween.
  • It should also be noted that the lower edge of the chamber 5 can be provided, for example, with a sealing joint 9 such as, for example, an O-ring and the chamber can be moved relative to this plate, as illustrated in FIGS. 1 and 2 using means for controlling the movement thereof, generally designated 10.
  • These control means may have any appropriate structure.
  • These means allow this chamber to be moved between the position illustrated in FIG. 1, in which the chamber and more particularly the base thereof and the sealing joint are in abutment against the analysis plate, thus preventing the absorption material from absorbing the cell fixing agent of the suspension contained in the chamber.
  • The chamber is held in this position for a predetermined period of time, for example, of approximately 30 minutes in order to allow a homogeneous deposit by cells being decanted on the analysis plate, as illustrated.
  • At the end of this predetermined period of time, the control means bring about a movement of the chamber from the position illustrated in FIG. 1 to the position illustrated in FIG. 2, in which this chamber is remote from the analysis plate, thus placing the fixing agent of the cell suspension and the absorption material in a fluid relationship.
  • Tests have shown that after a period of time of 30 minutes, the cells are already in the correct position and it is possible to initiate the absorption of the fixing agent in order to save time during the depositing operation of the cells on the analysis plate.
  • Of course, various embodiments of this system may be envisaged, in particular with respect to the movement means and the sealing means of the chamber.
  • It will be appreciated that this structure allows the cells to be deposited in a rapid and homogeneous manner on the analysis plate, in particular in the form of a single layer, which facilitates their subsequent analysis, and that this structure is particularly well suited to the analysis of cytological suspensions.

Claims (10)

1. System for depositing cells on an analysis plate (1), the cells being contained in a cell suspension (4) comprising a fixing agent and the cells, comprising, for receiving the suspension, a chamber (5) which is positioned above the analysis plate (1) and whose base is open and extends opposite a cell depositing zone of the plate and a material (7) for absorbing the fixing agent placed around the chamber and the depositing zone of the plate, characterised in that the chamber (5) is mounted so as to be able to be moved between a first position (FIG. 1) in abutment against the analysis plate (1) in order to allow the cells to be deposited on the depositing zone of the plate (1) by means of decantation and a second position (FIG. 2) remote from this analysis plate (1) in order to place the chamber (5) in a fluid relationship with the absorption material (7) in order to allow this material to absorb the fixing agent.
2. System according to claim 1, characterised in that the base of the chamber (5) is provided with sealing means (9).
3. System according to claim 1, characterised in that the chamber (5) is connected to means (10) for moving the chamber between its two positions at the end of a predetermined period of time.
4. System according to claim 3, characterised in that the predetermined period of time is approximately 30 minutes.
5. System according to claim 1, characterised in that the absorption material (7) is in the form of a sheet of absorbent paper which is placed around the depositing zone of the plate and the chamber (5).
6. System according to claim 2 characterised in that the chamber (5) is connected to means (10) for moving the chamber between its two positions at the end of a predetermined period of time.
7. System according to claim 6, characterised in that the predetermined period of time is approximately 30 minutes.
8. System according to claim 2, characterised in that the absorption material (7) is in the form of a sheet of absorbent paper which is placed around the depositing zone of the plate and the chamber (5).
9. System according to claim 3, characterised in that the absorption material (7) is in the form of a sheet of absorbent paper which is placed around the depositing zone of the plate and the chamber (5).
10. System according to claim 4, characterised in that the absorption material (7) is in the form of a sheet of absorbent paper which is placed around the depositing zone of the plate and the chamber (5).
US12/375,784 2006-07-31 2007-06-26 Cell deposition system Abandoned US20090325283A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0607005 2006-07-31
FR0607005A FR2904418B1 (en) 2006-07-31 2006-07-31 SYSTEM FOR DEPOSITING CELLS.
PCT/FR2007/001066 WO2008015321A1 (en) 2006-07-31 2007-06-26 Cell deposition system

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2007/001066 A-371-Of-International WO2008015321A1 (en) 2006-07-31 2007-06-26 Cell deposition system

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/357,868 Division US9261439B2 (en) 2006-07-31 2012-01-25 Cell deposition system

Publications (1)

Publication Number Publication Date
US20090325283A1 true US20090325283A1 (en) 2009-12-31

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Family Applications (2)

Application Number Title Priority Date Filing Date
US12/375,784 Abandoned US20090325283A1 (en) 2006-07-31 2007-06-26 Cell deposition system
US13/357,868 Expired - Fee Related US9261439B2 (en) 2006-07-31 2012-01-25 Cell deposition system

Family Applications After (1)

Application Number Title Priority Date Filing Date
US13/357,868 Expired - Fee Related US9261439B2 (en) 2006-07-31 2012-01-25 Cell deposition system

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US (2) US20090325283A1 (en)
EP (1) EP2047229B1 (en)
ES (1) ES2648796T3 (en)
FR (1) FR2904418B1 (en)
WO (1) WO2008015321A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020143997A (en) * 2019-03-06 2020-09-10 メディカテック株式会社 Settling chamber holder

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5318749A (en) * 1991-05-14 1994-06-07 Firma Andreas Hettich Centrifugation chamber for liquor testing
US5732287A (en) * 1995-06-07 1998-03-24 Polaroid Corporation Method, apparatus and system for cleaning pressure applying members
US5840584A (en) * 1995-06-07 1998-11-24 Waldenburg; Ottfried Blood in feces test device
US6627158B1 (en) * 1999-04-14 2003-09-30 Labonord Device for depositing cells on an analytical plate

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE8416418U1 (en) * 1984-08-23 Omega Medizintechnik GmbH & Co KG, 5270 Gummersbach Absorbent body with a central perforation opening for devices for cell sedimentation and in particular liquor sedimentation
DD287850A7 (en) * 1988-11-04 1991-03-14 Bezirksnervenklinik Schwerin,De ARRANGEMENT FOR PREPARING CELLAR FLUIDS FOR MICROSCOPIC STUDIES
FR2869413A1 (en) * 2004-04-21 2005-10-28 Michel Marie Francois Guiu Preparation of cytology plates in a liquid medium uses inclusion of absorbent material to filter preservation fluid

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5318749A (en) * 1991-05-14 1994-06-07 Firma Andreas Hettich Centrifugation chamber for liquor testing
US5732287A (en) * 1995-06-07 1998-03-24 Polaroid Corporation Method, apparatus and system for cleaning pressure applying members
US5840584A (en) * 1995-06-07 1998-11-24 Waldenburg; Ottfried Blood in feces test device
US6627158B1 (en) * 1999-04-14 2003-09-30 Labonord Device for depositing cells on an analytical plate

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020143997A (en) * 2019-03-06 2020-09-10 メディカテック株式会社 Settling chamber holder
JP7276817B2 (en) 2019-03-06 2023-05-18 メディカテック株式会社 settling chamber holder

Also Published As

Publication number Publication date
EP2047229A1 (en) 2009-04-15
FR2904418A1 (en) 2008-02-01
EP2047229B1 (en) 2017-10-11
WO2008015321A1 (en) 2008-02-07
US9261439B2 (en) 2016-02-16
FR2904418B1 (en) 2008-12-26
US20120122142A1 (en) 2012-05-17
ES2648796T3 (en) 2018-01-08

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Owner name: NOVACYT, FRANCE

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:PELTIER, ERIC ROGER, LEWIS;REEL/FRAME:022638/0296

Effective date: 20090116

STCB Information on status: application discontinuation

Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION