US20060276974A1 - System for remote viewing and display of a slide - Google Patents
System for remote viewing and display of a slide Download PDFInfo
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- US20060276974A1 US20060276974A1 US11/502,816 US50281606A US2006276974A1 US 20060276974 A1 US20060276974 A1 US 20060276974A1 US 50281606 A US50281606 A US 50281606A US 2006276974 A1 US2006276974 A1 US 2006276974A1
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- 238000000034 method Methods 0.000 claims abstract description 41
- 230000006835 compression Effects 0.000 claims description 11
- 238000007906 compression Methods 0.000 claims description 11
- 238000000386 microscopy Methods 0.000 claims description 5
- 230000003287 optical effect Effects 0.000 abstract description 6
- 239000002131 composite material Substances 0.000 abstract description 2
- 230000006837 decompression Effects 0.000 description 11
- 230000008859 change Effects 0.000 description 6
- 238000003384 imaging method Methods 0.000 description 6
- 230000008901 benefit Effects 0.000 description 3
- 238000001000 micrograph Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000013139 quantization Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011946 reduction process Methods 0.000 description 1
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
- G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T9/00—Image coding
- G06T9/007—Transform coding, e.g. discrete cosine transform
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16H—HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING OR PROCESSING OF MEDICAL OR HEALTHCARE DATA
- G16H10/00—ICT specially adapted for the handling or processing of patient-related medical or healthcare data
- G16H10/40—ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
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- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Multimedia (AREA)
- General Physics & Mathematics (AREA)
- Discrete Mathematics (AREA)
- Analytical Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Optics & Photonics (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Theoretical Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- General Health & Medical Sciences (AREA)
- Medical Informatics (AREA)
- Primary Health Care (AREA)
- Public Health (AREA)
- Microscoopes, Condenser (AREA)
Abstract
Description
- This is a divisional application filed from application Ser. No. 10/620,016, filed Jul. 14, 2003, which claims benefit of U.S. Provisional Application No. 60/087,523, filed Jun. 1, 1998, U.S. application Ser. No. 09/323,371, filed Jun. 1, 1999, and U.S. application Ser. No. 10/488,913, filed May 30, 2003. U.S. patent application Ser. No. 10/620,016 is incorporated herein by reference.
- This invention relates to a method and apparatus for viewing remote microscope images.
- Currently there is increasing demand for pathologist review of samples at remote locations. There exist multiple systems to address this need. They typically fall within one of two categories: live remote microscopy and virtual slide imaging.
- In live remote control microscopy, a user receives images that are taken from a slide on a microscope. In virtual slide imaging, a user receives images previously captured. Virtual slide systems take one or more images of an area of interest and assemble them together (if there is more than one image) to form a virtual slide. Each of these techniques has its advantages. Live remote imaging provides users with the closest approximation to manual manipulation. Virtual slides allow faster image viewing, since images are already captured.
- Virtual slide systems take one or more images and assemble them to form a “virtual slide.”
- However, users in the past were limited in their ability to integrate these technologies. One could only view and manipulate live and virtual images independently of one another. A user would have to clumsily go back and forth between these two modes of operation to separately look at the virtual slides and live microscope slides. We present a new method that integrates these ideas into one seamless operating environment.
- In accordance with the present invention, a method and apparatus are provided for the analysis of remote slides in a hybrid live and virtual medium. Users obtain benefits of each technique in a unified environment.
- A virtual slide is a digital representation of an area of interest of a microscopic slide. A virtual slide can be created multiple ways.
- One method is to use a motorized microscope that can move a specimen with respect to a microscope objective (e.g., a microscope with a motorized stage). With such system, one can capture one or more images through a microscope objective, such that a region of interest (all or part of the microscopic slide) is imaged. Each image is then joined together to form a composite or “virtual image.” Multiple methods of joining images together are known in the art. One example is when images are simply abutted one next to another. However, this method does not generally produce virtual slides without seams, because errors such as camera rotation relative to the axis of motion are difficult to correct. Even with submicron accuracy stages it is, in practice, difficult to obtain consistent positioning. Another method is to utilize overlap between adjacent images to edge align images for maximum seamlessness. This can be done by sequentially shifting overlapping regions in the x and/or y axis, for example, by a stepping motor, and calculating a correlation value (or measure of goodness of overlap). The shift which results in the best correlation value is then used to join the images together (
FIG. 1 ). While this method can be computationally expensive, it reduces reliance on difficult-to-attain mechanical positioning requirements and ultimately produces the best images in the sense of seamlessness. - In another method, the virtual slide is made simply by utilizing an imaging device with optics suitable to take a an image of the area of interest on the slide in one snapshot. This method is embodied in the form of a conventional digital or analog camera with a macro lens.
- This virtual slide can then be used to create a thumbnail view of the slide. To create the thumbnail view, the virtual slide is shrunk in resolution from its original, base resolution to a target resolution. If the target resolution is the same as the base resolution, then the image is unchanged. However, typically the resolution of the thumbnail desired is several times smaller than the base resolution.
- With the virtual slide created, the user may fully utilize the full capabilities of the remote microscope. The user is presented with an image window and a set of control features (
FIG. 2 ). Among these control features is a set of “optical objectives” and “virtual objectives.” - Optical objectives are images that are created by a camera digitizing an image through a microscope objective (e.g., 10x, 20x, or 40x) in real time (i.e., an image is captured at the time the user requests the image). Virtual objectives are digitally created magnifications created not by digitizing in real time, but rather by utilizing the existing virtual slide data to digitally create a field of view.
- When a user selects one of the optical objectives, a “change objective” command is sent to the microscope. This change objective command can also, specify additional qualifying information, such as microscope x, y, z positions, exposure setting, compression type and level, and image dimensions. If additional qualifying information is not sent, then the implicit qualifying information is the current state of the microscope or the last specified state. When the microscope receives the command, actions are taken to change the objective lens and to change the state of the microscope commensurate with the command (e.g., change the relative position of the objective lens relative to the microscopic slide, change exposure, etc.). An image is then digitized, compressed if so specified, and then transmitted to the user for display.
- When a user selects a virtual objective, a virtual objective command is sent to the microscope. Similar to an optical objective command, this virtual objective command can also specify additional qualifying information, such as microscope x, y, and/or z position. If additional qualifying information is not sent, then the implicit qualifying information is current microscope state or the last specified state. A region of interest is defined by the virtual request—it is the area on the microscope slide included in the field specified by the coordinates of the stage x, y and magnification of the command. This region of interest may optionally be trimmed such that image information already residing at the requesting user's view is not retransmitted to the user.
- An image of the region of interest can be created from the virtual slide in multiple ways. If the virtual slide is not compressed, the retrieval of image information corresponding to the region of interest can be done by simply copying data from the virtual slide. If the virtual slide is compressed, a region corresponding to at least the region of interest can be decompressed to a raw bitmap from the main compressed image. If the virtual slide was stored as multiple compressed images rather than one large image, additional efficiencies are possible. For example, only those images that contain the desired region of interest need be accessed for decompression, rather than the entire area of the virtual slide. This enhances performance.
- The decompression itself can also be performed in various ways. Strategies such as scaled decoding, as in the case of jpeg type compression, can be employed to improve the speed of the decompression by coupling a resolution reduction process with the decompression to speed up decompression when resolution reduction is required. Once the region of interest is decompressed, it can then be recompressed using a variety of strategies known to those skilled in the imaging field, which need not be the same as the method by which the virtual slide was compressed.
- An alternative type of decompression/recompression step can also be used involving partial decompression. Partial decompression, such as decoding of Huffman-encoded data, as in the case of jpeg, can be performed to produce raw coefficients, rather than full decompression, which produces a raw bitmap. The raw coefficients corresponding to the area of the region of interest can then be selected. These coefficients which correspond to the region of interest are then re-encoded. In the case of jpeg compression, this would involve re Huffman encoding of the coefficients, rather than in normal full compression, where a dct must be performed followed by quantization and then Huffman coding.
- Whichever the technique, the result is a compressed region of interest. The compressed region of interest is then transmitted to the user for viewing. The described method is more advantageous than sending the entire virtual slide, as one efficiently sends only that information required by the user.
- However, direct transfer of the compressed image without decompression is feasible when the virtual slide is stored as multiple compressed images. The compressed images that include the area specified by the region of interest can be directly transferred to the user, rather than going through a decompression/recompression step. The disadvantage is that one may transfer more information than is needed if, for example, the compressed images are at a higher resolution that the requested resolution. This can be partially solved by creation of multiple resolution versions of the virtual slide. There are also compression strategies available that allow only portions of the compressed images to be sent, such that a given resolution can be attained depending on which portions of the compressed image one chooses to send (e.g., progressive encoding). However, there is still the issue that the region of interest only partially covers the area of the compressed image. In this case, direct transfer of the image results in inefficiently sending data including both the region of interest and data outside the region of interest to the user.
- With this invention, users are afforded a streamlined method of utilizing the features of virtual and live microscopy techniques.
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FIG. 1 is a drawing showing the overlap between adjacent images during optimization of overlap. -
FIG. 2 is a photograph of a user's view of the remote microscope, showing a thumbnail view, microscope imaging window, and a set of microscope controls. - While the apparatus and methods of the present invention have been illustrated in terms of certain embodiments, the invention claimed herein is not limited to embodiments disclosed in this application. Rather, the scope of the invention is defined by the claims attached hereto.
- While the invention has been illustrated and explained herein in terms of certain embodiments the invention is not limited to the specific embodiments disclosed. Rather, the invention is defined by the scope of the claims appended hereto.
- A method for simultaneously viewing remote microscope images comprising virtual and live images and producing a seamless view of live and virtual images comprises: providing a virtual slide; providing a live microscope slide; and automatically and sequentially shifting overlapping regions of images from the slides to obtain the optimal image is disclosed.
Claims (34)
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
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US11/502,816 US20060276974A1 (en) | 2003-07-14 | 2006-08-11 | System for remote viewing and display of a slide |
US12/958,985 US20110187846A1 (en) | 2003-07-14 | 2010-12-02 | System for remote viewing and display of a slide |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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US10/620,016 US20040083085A1 (en) | 1998-06-01 | 2003-07-14 | Integrated virtual slide and live microscope system |
US11/502,816 US20060276974A1 (en) | 2003-07-14 | 2006-08-11 | System for remote viewing and display of a slide |
Related Parent Applications (1)
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US10/620,016 Division US20040083085A1 (en) | 1998-06-01 | 2003-07-14 | Integrated virtual slide and live microscope system |
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US12/958,985 Continuation US20110187846A1 (en) | 2003-07-14 | 2010-12-02 | System for remote viewing and display of a slide |
Publications (1)
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US20060276974A1 true US20060276974A1 (en) | 2006-12-07 |
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US11/502,816 Abandoned US20060276974A1 (en) | 2003-07-14 | 2006-08-11 | System for remote viewing and display of a slide |
US12/584,831 Expired - Fee Related US8036868B2 (en) | 1998-06-01 | 2009-09-11 | Intergrated systems and methods of virtual or live microscope slide presentation |
US12/958,985 Abandoned US20110187846A1 (en) | 2003-07-14 | 2010-12-02 | System for remote viewing and display of a slide |
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US10/620,016 Abandoned US20040083085A1 (en) | 1998-06-01 | 2003-07-14 | Integrated virtual slide and live microscope system |
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US12/584,831 Expired - Fee Related US8036868B2 (en) | 1998-06-01 | 2009-09-11 | Intergrated systems and methods of virtual or live microscope slide presentation |
US12/958,985 Abandoned US20110187846A1 (en) | 2003-07-14 | 2010-12-02 | System for remote viewing and display of a slide |
Country Status (2)
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WO (1) | WO2005008297A2 (en) |
Cited By (4)
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US20060251331A1 (en) * | 1998-06-01 | 2006-11-09 | Trestle Acquisition Corp. | System and method for reconstructing a diagnostic trajectory |
US20100073472A1 (en) * | 1998-06-01 | 2010-03-25 | Carl Zeiss Microlmaging Ais, Inc. | Intergrated virtual slide and live microscope system |
US20110083193A1 (en) * | 2009-10-06 | 2011-04-07 | At&T Intellectual Property I, L.P. | Remote viewing of multimedia content |
US20120320094A1 (en) * | 2011-06-16 | 2012-12-20 | The Leeds Teaching Hospitals Nhs Trust | Virtual microscopy |
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US7027628B1 (en) * | 2000-11-14 | 2006-04-11 | The United States Of America As Represented By The Department Of Health And Human Services | Automated microscopic image acquisition, compositing, and display |
AU2010222633B2 (en) | 2009-03-11 | 2015-05-14 | Sakura Finetek Usa, Inc. | Autofocus method and autofocus device |
WO2011017041A2 (en) | 2009-07-27 | 2011-02-10 | Corista LLC | System for networked digital pathology exchange |
US8504726B2 (en) | 2009-07-27 | 2013-08-06 | Corista LLC | User targeted medical imaging and information packaging, compression and distribution system |
US10139613B2 (en) | 2010-08-20 | 2018-11-27 | Sakura Finetek U.S.A., Inc. | Digital microscope and method of sensing an image of a tissue sample |
JP2013153429A (en) * | 2011-12-27 | 2013-08-08 | Canon Inc | Image processing apparatus, image display system, image processing method and image processing program |
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US10007102B2 (en) | 2013-12-23 | 2018-06-26 | Sakura Finetek U.S.A., Inc. | Microscope with slide clamping assembly |
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US11280803B2 (en) | 2016-11-22 | 2022-03-22 | Sakura Finetek U.S.A., Inc. | Slide management system |
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Also Published As
Publication number | Publication date |
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WO2005008297A2 (en) | 2005-01-27 |
WO2005008297A9 (en) | 2005-05-12 |
US20110187846A1 (en) | 2011-08-04 |
US8036868B2 (en) | 2011-10-11 |
US20040083085A1 (en) | 2004-04-29 |
WO2005008297A3 (en) | 2005-03-31 |
US20100073472A1 (en) | 2010-03-25 |
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