DE69939902D1 - Wellenlängen verschiebende proben und primer - Google Patents

Wellenlängen verschiebende proben und primer

Info

Publication number
DE69939902D1
DE69939902D1 DE69939902T DE69939902T DE69939902D1 DE 69939902 D1 DE69939902 D1 DE 69939902D1 DE 69939902 T DE69939902 T DE 69939902T DE 69939902 T DE69939902 T DE 69939902T DE 69939902 D1 DE69939902 D1 DE 69939902D1
Authority
DE
Germany
Prior art keywords
primers
harvester
probes
fluorescence
emitter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69939902T
Other languages
English (en)
Inventor
Sanjay Tyagi
Fred R Kramer
Salvatore A Marras
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PHRI Properties Inc
Original Assignee
PHRI Properties Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22410742&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69939902(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by PHRI Properties Inc filed Critical PHRI Properties Inc
Application granted granted Critical
Publication of DE69939902D1 publication Critical patent/DE69939902D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
DE69939902T 1998-07-28 1999-07-28 Wellenlängen verschiebende proben und primer Expired - Lifetime DE69939902D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/123,764 US6037130A (en) 1998-07-28 1998-07-28 Wavelength-shifting probes and primers and their use in assays and kits
PCT/US1999/017145 WO2000006778A1 (en) 1998-07-28 1999-07-28 Wavelength-shifting probes and primers

Publications (1)

Publication Number Publication Date
DE69939902D1 true DE69939902D1 (de) 2008-12-24

Family

ID=22410742

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69939902T Expired - Lifetime DE69939902D1 (de) 1998-07-28 1999-07-28 Wellenlängen verschiebende proben und primer

Country Status (8)

Country Link
US (1) US6037130A (de)
EP (1) EP1100971B1 (de)
JP (1) JP3851088B2 (de)
AT (1) ATE414170T1 (de)
AU (1) AU763106B2 (de)
CA (1) CA2336489C (de)
DE (1) DE69939902D1 (de)
WO (1) WO2000006778A1 (de)

Families Citing this family (153)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6576419B1 (en) * 1993-07-23 2003-06-10 University Of Utah Research Foundation Assay procedure using fluorogenic tracers
US20070269799A9 (en) * 1994-06-22 2007-11-22 Zhang David Y Nucleic acid amplification methods
US20020055101A1 (en) 1995-09-11 2002-05-09 Michel G. Bergeron Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
DE69734576T2 (de) * 1996-03-01 2006-08-03 E.I. Dupont De Nemours And Co., Wilmington Methode zur vervielfältigung und zum nachweis eines gewünschten nukleinsäurefragments
US20100267012A1 (en) * 1997-11-04 2010-10-21 Bergeron Michel G Highly conserved genes and their use to generate probes and primers for detection of microorganisms
US20030049636A1 (en) 1999-05-03 2003-03-13 Bergeron Michel G. Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories
US7312302B2 (en) 1997-02-20 2007-12-25 Oncolmmunin, Inc. Compositions for the detection of enzyme activity in biological samples and methods of use thereof
US6037137A (en) * 1997-02-20 2000-03-14 Oncoimmunin, Inc. Fluorogenic peptides for the detection of protease activity
US6893868B2 (en) * 1997-02-20 2005-05-17 Onco Immunin, Inc. Homo-doubly labeled compositions for the detection of enzyme activity in biological samples
ATE426018T1 (de) * 1997-04-10 2009-04-15 Stichting Katholieke Univ Pca3, pca3-gene und verfahren zu ihrer verwendung
US20030165888A1 (en) * 2001-07-18 2003-09-04 Brown Bob D. Oligonucleotide probes and primers comprising universal bases for diagnostic purposes
US6518017B1 (en) * 1997-10-02 2003-02-11 Oasis Biosciences Incorporated Combinatorial antisense library
US7595189B2 (en) * 1999-01-08 2009-09-29 Applied Biosystems, Llc Integrated optics fiber array
AU2965500A (en) * 1999-01-15 2000-08-01 Gene Logic, Inc. Immobilized nucleic acid hybridization reagent and method
US6951722B2 (en) * 1999-03-19 2005-10-04 Takara Bio Inc. Method for amplifying nucleic acid sequence
EP1173614A4 (de) * 1999-04-08 2003-10-29 Oasis Biosciences Inc Antisense oligonukleotide enthaltend universelle und/oder degenerierte basen
US6680377B1 (en) * 1999-05-14 2004-01-20 Brandeis University Nucleic acid-based detection
WO2000070329A1 (en) * 1999-05-14 2000-11-23 Brandeis University Nucleic acid-based detection
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
NZ516278A (en) * 1999-06-22 2004-03-26 Invitrogen Corp Improved primers and methods for the detection and discrimination of nucleic acids
US6830902B1 (en) 1999-07-02 2004-12-14 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
AU6068300A (en) 1999-07-06 2001-01-22 Caliper Technologies Corporation Microfluidic systems and methods for determining modulator kinetics
US6692915B1 (en) * 1999-07-22 2004-02-17 Girish N. Nallur Sequencing a polynucleotide on a generic chip
US6440706B1 (en) 1999-08-02 2002-08-27 Johns Hopkins University Digital amplification
WO2001012855A2 (en) * 1999-08-13 2001-02-22 Yale University Binary encoded sequence tags
US6586177B1 (en) * 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
CA2905326C (en) * 1999-09-28 2016-09-27 Geneohm Sciences Canada Inc. Nucleic acids and methods for the detection of klebsiella
ES2260059T3 (es) * 1999-09-29 2006-11-01 Diagnocure Inc. Rna mensajero del pca3 en tejidos benignos y malignos de prostata.
AU781945B2 (en) * 1999-10-22 2005-06-23 Phri Properties, Inc. Assays for short sequence variants
US6692965B1 (en) * 1999-11-23 2004-02-17 Chromocell Corporation Isolation of living cells and preparation of cell lines based on detection and quantification of preselected cellular ribonucleic acid sequences
US6919174B1 (en) * 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
EP1294939B1 (de) * 2000-04-03 2010-01-27 Cytyc Corporation Nachweis und typisierung des papillomavirus mittels pna-sonden
US6936443B2 (en) * 2000-04-03 2005-08-30 Cytyc Corporation Detection and typing of human papillomavirus using PNA probes
WO2001083820A1 (en) 2000-04-28 2001-11-08 Montclair Group Methods and compositions for the manufacture of molecular beacons
US7019129B1 (en) * 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
US6887664B2 (en) 2000-06-06 2005-05-03 Applera Corporation Asynchronous primed PCR
US6323009B1 (en) * 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
GB2368809B (en) 2000-09-15 2004-09-29 Norchip As Microfabricated reaction chamber system
US6815164B2 (en) 2000-10-06 2004-11-09 Nugen Technologies, Inc. Methods and probes for detection and/or quantification of nucleic acid sequences
AU2002232538A1 (en) * 2000-12-19 2002-07-01 Discoverx Strand displacement detection of target nucleic acid
US6573051B2 (en) * 2001-03-09 2003-06-03 Molecular Staging, Inc. Open circle probes with intramolecular stem structures
FR2822234B1 (fr) * 2001-03-14 2003-06-13 Univ Pasteur Procede de determination locale de parametres structuraux de molecules, notamment biochimiques ou pharmacologiques et utilisations dudit procede
EP1390544B1 (de) * 2001-04-17 2006-11-29 The New York Blood Center, Inc. Universelles nachweissystem für mehrere varianten
CA2446206A1 (en) * 2001-05-03 2002-11-14 Warnex Research Inc. A molecular tag code for monitoring a product and process using same
WO2003000917A2 (en) * 2001-06-21 2003-01-03 The Regents Of The University Of California Electrochemical detection of mismatch nucleic acids
US7297494B2 (en) * 2001-06-25 2007-11-20 Georgia Tech Research Corporation Activatable probes and methods for in vivo gene detection
CA2451614C (en) * 2001-06-25 2011-01-04 Georgia Tech Research Corporation Dual resonance energy transfer nucleic acid probes
GB2378245A (en) * 2001-08-03 2003-02-05 Mats Nilsson Nucleic acid amplification method
US20030082547A1 (en) 2001-08-27 2003-05-01 Ewing Gregory J. Non-fluorescent quencher compounds and biomolecular assays
AU2002331777A1 (en) * 2001-08-30 2003-03-18 Spectral Genomics, Inc. Arrays comprising pre-labeled biological molecules and methods for making and using these arrays
US20030105320A1 (en) * 2001-08-31 2003-06-05 Becker Michael M. Affinity-shifted probes for quantifying analyte polynucleotides
US7070933B2 (en) * 2001-09-28 2006-07-04 Gen-Probe Incorporated Inversion probes
AU2002334874B2 (en) * 2001-10-09 2007-09-06 3-Dimensional Pharmaceuticals, Inc. Substituted diphenyloxazoles, the synthesis thereof, and the use thereof as fluorescence probes
US20030165859A1 (en) * 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
EP1444365A4 (de) * 2001-10-25 2005-07-20 Gorilla Genomics Inc Asymmetrische pcr mit nukleasefreier polymerase oder nukleaseresistenten molecular beacons
GB0200526D0 (en) * 2002-01-11 2002-02-27 Science And Technology The Methods
US7553619B2 (en) * 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
CA2482767A1 (en) * 2002-04-22 2003-10-30 Joseph F. Lawler, Jr. Reagents for monitoring nuclei acid amplification and methods of using same
AU2003234602A1 (en) * 2002-05-13 2003-11-11 Nanocytometry Corporation Oligonucleotide probes for in vitro, in vivo and intracellular detection
AU2003267960A1 (en) * 2002-05-22 2004-01-06 Marshall University Methods, probes, and accessory molecules for detecting single nucleotide polymorphisms
JP4457001B2 (ja) * 2002-05-31 2010-04-28 セクレタリー・デパートメント・オブ・アトミック・エナジー ドナー／アクセプター部分が相補鎖上となる標的核酸検出のｍｅｔ／ｆｒｅｔに基づく方法
WO2007116408A2 (en) * 2006-04-11 2007-10-18 Nanodiagnostics Israel Ltd. Pluripotent stem cells characterized by expression of germline specific genes
CA2493768A1 (en) * 2002-07-23 2004-01-29 Nanodiagnostics, Inc. Embryonic stem cell markers and uses thereof
CA2433473A1 (en) * 2002-07-23 2004-01-23 F. Hoffmann-La Roche Ag Fluorescent hybridization probes with reduced background
US7582470B2 (en) * 2002-07-31 2009-09-01 Gen-Probe Incorporated Device for amplifying and detecting a target nucleic acid
US7541193B2 (en) * 2002-11-18 2009-06-02 Panomics, Inc. Caged sensors, regulators and compounds and uses thereof
US20040121338A1 (en) * 2002-12-19 2004-06-24 Alsmadi Osama A. Real-time detection of rolling circle amplification products
US20050042639A1 (en) * 2002-12-20 2005-02-24 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA length
US8275554B2 (en) * 2002-12-20 2012-09-25 Caliper Life Sciences, Inc. System for differentiating the lengths of nucleic acids of interest in a sample
US9487823B2 (en) * 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
ATE386141T1 (de) * 2002-12-20 2008-03-15 Stratagene Inc Zusammensetzungen und verfahren für den nachweis von polynukleotiden
EP1587940A4 (de) 2002-12-20 2006-06-07 Caliper Life Sciences Inc Einzelmolekül amplifikation und dna-nachweis
WO2004068112A2 (en) * 2003-01-28 2004-08-12 Gorilla Genomics, Inc. Hairpin primer amplification
WO2004070056A2 (en) 2003-02-07 2004-08-19 Diagnocure Inc. Method to detect prostate cancer in a sample
CN100439515C (zh) * 2003-03-03 2008-12-03 清华大学 一种核酸分析芯片实验室系统与应用
US7238792B2 (en) * 2003-03-18 2007-07-03 Washington State University Research Foundation Foldable polymers as probes
US8043834B2 (en) * 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
US20040248103A1 (en) * 2003-06-04 2004-12-09 Feaver William John Proximity-mediated rolling circle amplification
CN103898207B (zh) * 2003-08-01 2016-09-14 戴诺生物技术有限公司 自杂交多重靶核酸探针及其使用方法
KR20150005720A (ko) * 2004-02-18 2015-01-14 크로모셀 코포레이션 신호 프로브를 이용하는 방법 및 물질
CA2585525A1 (en) * 2004-04-20 2005-11-03 Genaco Biomedical Products, Inc. Method for detecting ncrna
US7575863B2 (en) * 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
US7635598B2 (en) * 2004-07-08 2009-12-22 Biosearch Technologies, Inc. Inducible fluorescence assay
US7361469B2 (en) * 2004-08-13 2008-04-22 Stratagene California Dual labeled fluorescent probes
DE602005025782D1 (de) * 2004-09-21 2011-02-17 Life Technologies Corp Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas)
US20060194222A1 (en) * 2004-10-20 2006-08-31 Stratagene California Triplex probe compositions and methods for polynucleotide detection
US20060275792A1 (en) * 2004-11-15 2006-12-07 Lee Jun E Enhancement of nucleic acid amplification using double-stranded DNA binding proteins
US20060105348A1 (en) * 2004-11-15 2006-05-18 Lee Jun E Compositions and methods for the detection and discrimination of nucleic acids
US7842794B2 (en) 2004-12-17 2010-11-30 Roche Molecular Systems, Inc. Reagents and methods for detecting Neisseria gonorrhoeae
CA2491067A1 (en) 2004-12-24 2006-06-24 Stichting Katholieke Universiteit Mrna rations in urinary sediments and/or urine as a prognostic marker for prostate cancer
AU2006213907A1 (en) * 2005-02-09 2006-08-17 Stratagene California Key probe compositions and methods for polynucleotide detection
US8309303B2 (en) * 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
GB2424886A (en) * 2005-04-04 2006-10-11 Dxs Ltd Polynucleotide primers against epidermal growth factor receptor and method of detecting gene mutations
US20070213293A1 (en) * 2005-04-08 2007-09-13 Nastech Pharmaceutical Company Inc. Rnai therapeutic for respiratory virus infection
AU2006235364A1 (en) 2005-04-08 2006-10-19 Marina Biotech, Inc. RNAi therapeutic for respiratory virus infection
WO2006119326A2 (en) * 2005-05-02 2006-11-09 Stratagene California Oligonucleotide probe/primer compositions and methods for polynucleotide detection
WO2006135765A1 (en) * 2005-06-09 2006-12-21 Epoch Biosciences, Inc. Improved primer-based amplification methods
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
ES2371088T3 (es) 2005-10-05 2011-12-27 F. Hoffmann-La Roche Ag Transferencia de energía no fluorescente.
US7993881B2 (en) * 2005-11-15 2011-08-09 Genoid Kft Method for detecting pathogens using molecular beacons
WO2007060280A1 (en) * 2005-11-25 2007-05-31 Hidex Oy Homogeneous luminescence bioassay
CA3046754A1 (en) 2005-11-29 2007-06-07 Cambridge Enterprise Limited Markers for breast cancer including refsnp_id 3817198
US20110143344A1 (en) * 2006-03-01 2011-06-16 The Washington University Genetic polymorphisms and substance dependence
US8080371B2 (en) * 2006-03-01 2011-12-20 The Washington University Markers for addiction
EP2004847B1 (de) * 2006-03-16 2017-05-03 Pentabase Aps Oligonukleotide mit signalpaaren und hydrophoben nukleotiden, stammlosen leuchttürmen zur detektion von nukleinsäuren, methylierungsstatus und mutanten von nukleinsäuren
RU2394915C2 (ru) * 2006-03-24 2010-07-20 Александр Борисович Четверин Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления
US8313903B2 (en) 2006-03-31 2012-11-20 The Trustees Of Columbia University In The City Of New York Binary DNA probe for fluorescent analysis of nucleic acids
FR2900158A1 (fr) * 2006-04-25 2007-10-26 Biomerieux Sa Nouvelle sonde de detection agissant par reconnaissance moleculaire
EP3357490A1 (de) 2006-04-28 2018-08-08 Children's Hospital Medical Center Fusogene eigenschaften von saposin c und verwandten proteinen und polypeptiden zur anwendung auf transmembranen wirkstoffverabreichungssystemen
DE102006020885A1 (de) * 2006-05-05 2007-11-08 Qiagen Gmbh Einführung von Sequenzelementen in Nukleinsäuren
EP2027251A4 (de) 2006-05-17 2010-05-05 California Inst Of Techn Thermisches wechselbeanspruchungssystem
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
US7972786B2 (en) * 2006-07-07 2011-07-05 Brandeis University Detection and analysis of influenza virus
US9045522B2 (en) 2006-07-31 2015-06-02 Wanli Bi Nucleic acid amplification using a reversibly modified oligonucleotide
WO2008016562A2 (en) * 2006-07-31 2008-02-07 Wanli Bi Nucleic acid amplification using a reversibly modified oligonucleotide
EP2484781B1 (de) * 2006-08-01 2017-08-23 Applied Biosystems, LLC Nachweis von Analyten und Nukleinsäuren
JP2010502573A (ja) 2006-08-11 2010-01-28 ユニヴァーシティ オブ メディスン アンド デンティストリー オブ ニュー ジャージー 二重増感剤含有ルミネッセンス化合物および結合体、ならびにその使用
FR2906532B1 (fr) * 2006-09-28 2008-12-12 Biomerieux Sa Nouvel oligonucleotide marque
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
FI20070163A0 (fi) 2007-02-27 2007-02-27 Hidex Oy Parannettu homogeeninen luminesenssibiotesti
US8367899B2 (en) 2007-12-31 2013-02-05 E I Du Pont De Neumours And Company Gray leaf spot tolerant maize and methods of production
US9116147B2 (en) * 2008-02-28 2015-08-25 Board Of Regents, The University Of Texas System Compositions and methods for detection of small molecules using dyes derivatized with analyte responsive receptors in a chemiluminescent assay
PT2757091T (pt) * 2008-04-01 2017-07-24 Biosearch Tech Inc Sondas de fluoróforo e supressor de fluorescência de ácido nucleico estabilizadas
US20090270269A1 (en) * 2008-04-28 2009-10-29 Ashok Kumar Nano-scale fluoro-biosensors exhibiting a low false alarm rate for rapid detection of biological contaminants
AU2009246363B2 (en) * 2008-05-13 2013-10-24 Gen-Probe Incorporated Inactivatable target capture oligomers for use in the selective hybridization and capture of target nucleic acid sequences
FR2933410B1 (fr) * 2008-07-04 2010-08-20 Biomerieux Sa Nouvelle sonde de detection
WO2010011884A2 (en) * 2008-07-25 2010-01-28 University Of Florida Research Foundation, Inc. Novel nucleic acid-based molecular probes
GB0912509D0 (en) 2009-07-17 2009-08-26 Norchip As A microfabricated device for metering an analyte
WO2011011426A2 (en) 2009-07-20 2011-01-27 Bar Harbor Biotechnology, Inc. Methods for assessing disease risk
WO2011011535A2 (en) 2009-07-21 2011-01-27 Gen-Probe Incorporated Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range
US8815515B1 (en) 2009-09-24 2014-08-26 University Of Utah Research Foundation Methods, compositions, and kits for rare allele detection
WO2011088193A2 (en) 2010-01-13 2011-07-21 University Of Medicine And Dentistry Of New Jersey Fluorophore chelated lanthanide luminiscent probes with improved quantum efficiency
US9970021B2 (en) 2010-01-26 2018-05-15 Pioneer Hi-Bred International, Inc. HPPD-inhibitor herbicide tolerance
EP2612910A4 (de) * 2010-09-01 2015-05-06 Nec Corp Verfahren zur erkennung einer zielsubstanz, dafür verwendeter aptamer-satz sowie sensor und vorrichtung dafür
CA2822955A1 (en) 2010-12-30 2012-07-05 Pioneer Hi-Bred International, Inc. Methods of identifying aphid resistant soybeans
ES2632768T3 (es) 2011-01-17 2017-09-15 Life Technologies Corporation Flujo de trabajo para la detección de ligandos empleando ácidos nucleicos
ES2805874T3 (es) 2011-01-17 2021-02-15 Life Technologies Corp Ligación enzimática de ácidos nucleicos
BR112013026451B1 (pt) 2011-04-15 2021-02-09 Becton, Dickinson And Company sistema e método para realizar ensaios de diagnóstico molecular em várias amostras em paralelo e simultaneamente amplificação em tempo real em pluralidade de câmaras de reação de amplificação
WO2013067202A1 (en) 2011-11-04 2013-05-10 Handylab, Inc. Polynucleotide sample preparation device
WO2013101750A1 (en) 2011-12-29 2013-07-04 Pioneer Hi-Bred International, Inc. Methods of improving aphid resistance in soybeans
US20130337442A1 (en) 2012-06-15 2013-12-19 Pioneer Hi-Bred International, Inc. Genetic loci associated with soybean cyst nematode resistance and methods of use
US10301378B2 (en) 2013-06-18 2019-05-28 New York University Cellular factors involved in the cytotoxicity of Staphylococcus aureus leukocidins: novel therapeutic targets
US20160272997A1 (en) 2013-10-25 2016-09-22 Pioneer Hi-Bred International, Inc. Stem canker tolerant soybeans and methods of use
EP3063317B1 (de) 2013-10-28 2020-06-03 DOTS Technology Corp. Allergennachweis
CA2966641A1 (en) 2015-01-29 2016-08-04 Pioneer Hi-Bred International, Inc. Polynucleotides and kits associated with soybean iron deficiency tolerance and methods of detection and breeding
WO2016161375A2 (en) 2015-04-03 2016-10-06 University Of Massachusetts Methods of using oligonucleotide-guided argonaute proteins
CA3002670A1 (en) 2015-12-18 2017-06-22 Donald Earl Kyle Genetic loci associated with reproductive growth phenotypes in soybean and methods of use
CA3004914A1 (en) 2016-02-05 2017-08-10 Pioneer Hi-Bred International, Inc. Genetic loci associated with brown stem rot resistance in soybean and methods of use
BR112018016164A2 (pt) 2016-02-11 2019-01-22 Pioneer Hi Bred Int método de seleção de uma primeira planta ou germoplasma de soja, plasma ou germoplasma de soja introgredido e kit para selecionar pelo menos uma planta de soja
US10718017B2 (en) * 2016-03-31 2020-07-21 Arizona Board Of Regents On Behalf Of Arizona State University Probes and methods for measuring tandem repeats
BR112018073236A2 (pt) 2016-05-12 2019-02-19 Pioneer Hi Bred Int métodos para genotipagem agrupada simultânea
US11242568B2 (en) 2017-12-29 2022-02-08 City Of Hope DNA methylation diagnostic test for breast cancer

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4261968A (en) * 1979-05-10 1981-04-14 Syva Company Fluorescence quenching with immunological pairs in immunoassays
CA1190838A (en) * 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
US5241060A (en) * 1982-06-23 1993-08-31 Enzo Diagnostics, Inc. Base moiety-labeled detectable nucleatide
CA1223831A (en) * 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
US5260433A (en) * 1982-06-23 1993-11-09 Enzo Diagnostics, Inc. Saccharide specific binding system labeled nucleotides
US4766062A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
DE3688965T2 (de) * 1985-05-02 1994-03-24 Genetics Inst Verfahren und aufbau einer nukleinsäure zur erzeugung von reagenzienkomplexen zur verwendung bei der bestimmung von zielnukleotidsequenzen.
US4822733A (en) * 1985-05-28 1989-04-18 Amoco Corporation Lifetime-resolved assay procedures
US4725537A (en) * 1985-09-19 1988-02-16 Allied Corporation Assay, reagent and kit employing nucleic acid strand displacement and restriction endonuclease cleavage
US4725536A (en) * 1985-09-19 1988-02-16 Genetics Institute, Inc. Reagent polynucleotide complex with multiple target binding regions, and kit and methods
US4752566A (en) * 1985-12-17 1988-06-21 Genetics Institute, Inc. Displacement polynucleotide method and reagent complex employing labeled probe polynucleotide
DE3785591T2 (de) * 1986-01-10 1993-09-02 Amoco Corp Kompetitiver homogener test.
US5082830A (en) * 1988-02-26 1992-01-21 Enzo Biochem, Inc. End labeled nucleotide probe
US5118801A (en) * 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
CA1339731C (en) * 1988-10-12 1998-03-17 Charles T. Caskey Multiplex genomic dna amplification for deletion detection
US5401847A (en) * 1990-03-14 1995-03-28 Regents Of The University Of California DNA complexes with dyes designed for energy transfer as fluorescent markers
US5312921A (en) * 1990-03-14 1994-05-17 Regents Of The University Of California Dyes designed for high sensitivity detection of double-stranded DNA
ATE199157T1 (de) * 1990-03-14 2001-02-15 Univ California Multichromophore fluoreszierende sonden
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
JP3085756B2 (ja) * 1991-10-30 2000-09-11 株式会社日立製作所 核酸の検出方法
US5411875A (en) * 1991-11-01 1995-05-02 University Of Iowa Research Foundation Method for retrieval of unknown flanking DNA sequence
EP1067134B1 (de) * 1991-11-07 2004-07-28 Nanotronics, Inc. Hybridisierung von mit Chromophoren und Fluorophoren konjugierten Polynukleotiden zur Erzeugung eines Donor-Donor Energietransfersystems
US5348853A (en) * 1991-12-16 1994-09-20 Biotronics Corporation Method for reducing non-specific priming in DNA amplification
US5332659A (en) * 1992-04-09 1994-07-26 The United States Of America As Represented By The Secretary Of The Navy Light emission-or absorbance-based binding assays for polynucleic acids
EP0601889A2 (de) * 1992-12-10 1994-06-15 Maine Medical Center Research Institute Nukleinsäure-Sonden
CA2129787A1 (en) * 1993-08-27 1995-02-28 Russell G. Higuchi Monitoring multiple amplification reactions simultaneously and analyzing same
EP0728218B1 (de) * 1993-11-12 2008-01-02 PHRI Properties, Inc. Hybridisierungsprobe zur nukleinsäuredetektion, universelle stammstrukturen, methoden und reagentiensätze
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5654419A (en) * 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5622821A (en) * 1994-06-29 1997-04-22 The Regents Of The University Of California Luminescent lanthanide chelates and methods of use
US5565554A (en) * 1994-07-29 1996-10-15 The Regents Of The University Of California Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores
US5491063A (en) * 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
US5571673A (en) * 1994-11-23 1996-11-05 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
US5863504A (en) * 1995-03-16 1999-01-26 Bio-Rad Laboratories, Inc. Fluorescence imaging instrument utilizing fish
US5728528A (en) * 1995-09-20 1998-03-17 The Regents Of The University Of California Universal spacer/energy transfer dyes
DE69738687D1 (de) * 1996-04-12 2008-06-26 Phri Properties Inc Sonden, kits und assays
WO1998010096A1 (en) * 1996-04-12 1998-03-12 The Public Health Research Institute Of The City Of New York, Inc. Detection probes, kits and assays
ATE246256T1 (de) * 1998-06-12 2003-08-15 Intergen Company Mehrfach fluoreszierende und energie übertragende hairpin-oligonukleotide

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CA2336489C (en) 2011-05-03
AU763106B2 (en) 2003-07-10
CA2336489A1 (en) 2000-02-10
EP1100971A1 (de) 2001-05-23
EP1100971A4 (de) 2004-11-24
WO2000006778A1 (en) 2000-02-10
JP3851088B2 (ja) 2006-11-29
ATE414170T1 (de) 2008-11-15
AU5240299A (en) 2000-02-21
JP2002521069A (ja) 2002-07-16
US6037130A (en) 2000-03-14

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