DE69610304T2 - Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay - Google Patents

Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay

Info

Publication number
DE69610304T2
DE69610304T2 DE69610304T DE69610304T DE69610304T2 DE 69610304 T2 DE69610304 T2 DE 69610304T2 DE 69610304 T DE69610304 T DE 69610304T DE 69610304 T DE69610304 T DE 69610304T DE 69610304 T2 DE69610304 T2 DE 69610304T2
Authority
DE
Germany
Prior art keywords
oligonucleotide
methods
reagents
probe
polymerase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69610304T
Other languages
English (en)
Other versions
DE69610304D1 (de
Inventor
Paul E Mayrand
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Publication of DE69610304D1 publication Critical patent/DE69610304D1/de
Application granted granted Critical
Publication of DE69610304T2 publication Critical patent/DE69610304T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
DE69610304T 1995-05-05 1996-04-05 Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay Expired - Lifetime DE69610304T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US43550995A 1995-05-05 1995-05-05
PCT/US1996/004693 WO1996034983A1 (en) 1995-05-05 1996-04-05 Methods and reagents for combined pcr amplification and hybridization probing assay

Publications (2)

Publication Number Publication Date
DE69610304D1 DE69610304D1 (de) 2000-10-19
DE69610304T2 true DE69610304T2 (de) 2001-04-05

Family

ID=23728690

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69610304T Expired - Lifetime DE69610304T2 (de) 1995-05-05 1996-04-05 Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay

Country Status (8)

Country Link
US (8) US5691146A (de)
EP (1) EP0826066B1 (de)
JP (3) JP4040087B2 (de)
AT (1) ATE196322T1 (de)
AU (1) AU693023B2 (de)
CA (1) CA2219891C (de)
DE (1) DE69610304T2 (de)
WO (1) WO1996034983A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102010003781A1 (de) * 2010-04-08 2011-12-15 Aj Innuscreen Gmbh Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen

Families Citing this family (140)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6787304B1 (en) 1994-12-28 2004-09-07 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US20030165908A1 (en) * 1994-12-30 2003-09-04 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US5801155A (en) * 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
WO1996034983A1 (en) 1995-05-05 1996-11-07 The Perkin-Elmer Corporation Methods and reagents for combined pcr amplification and hybridization probing assay
US6706471B1 (en) * 1996-01-24 2004-03-16 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US7070925B1 (en) 1996-07-16 2006-07-04 Gen-Probe Incorporated Method for determining the presence of an RNA analyte in a sample using a modified oligonucleotide probe
DE69736667T2 (de) * 1996-07-16 2007-09-06 Gen-Probe Inc., San Diego Verfahren zum nachweis und amplifikation von nukleinsäuresequenzen unter verbrauch von modifizierten oligonukleotiden mit erhöhter zielschmelztemperatur (tm)
US5853990A (en) 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
GB2333597B (en) 1996-10-29 2001-04-18 Univ Nebraska At Lincoln Method for detecting point mutations in dna utilizing fluorescence energy transfer
US6017702A (en) * 1996-12-05 2000-01-25 The Perkin-Elmer Corporation Chain-termination type nucleic acid sequencing method including 2'-deoxyuridine-5'-triphosphate
US5846729A (en) * 1997-02-27 1998-12-08 Lorne Park Research, Inc. Assaying nucleotides in solution using a fluorescent intensity quenching effect
US6060242A (en) * 1997-02-27 2000-05-09 Lorne Park Research, Inc. PNA diagnostic methods
US6251591B1 (en) 1997-02-27 2001-06-26 Lorne Park Research, Inc. Quantitative method for detecting nucleotide concentration
JP3016759B2 (ja) * 1997-02-28 2000-03-06 スミスクライン・ビーチャム・コーポレイション 競争的ハイブリダイゼーションによる蛍光エネルギー転移
EP1666609B1 (de) * 1997-02-28 2012-09-26 Quest Diagnostics Investments Incorporated Fluoreszens-Energie-Transfer durch kompetitive Hybridisierung
US7803528B1 (en) 1997-02-28 2010-09-28 Quest Diagnostics Incorporated Fluorescence energy transfer by competitive hybridization
AU9190098A (en) * 1997-09-18 1999-04-05 Erasmus Universiteit Rotterdam Detection of minimal residual disease in lymphoid malignancies
US5935791A (en) * 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
FR2769323B1 (fr) * 1997-10-08 2001-07-13 Suez Lyonnaise Des Eaux Moyens pour l'analyse qualitative et quantitative des populations microbiennes eventuellement presentes dans un echantillon
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
AU1366299A (en) 1997-10-27 1999-05-17 Boston Probes, Inc. Methods, kits and compositions pertaining to pna molecular beacons
US6080868A (en) 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
GB9803382D0 (en) 1998-02-19 1998-04-15 Secr Defence Detection system
US6268146B1 (en) 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US6312902B1 (en) 1998-03-13 2001-11-06 Promega Corporation Nucleic acid detection
US6391551B1 (en) 1998-03-13 2002-05-21 Promega Corporation Detection of nucleic acid hybrids
US6703211B1 (en) 1998-03-13 2004-03-09 Promega Corporation Cellular detection by providing high energy phosphate donor other than ADP to produce ATP
US6277578B1 (en) 1998-03-13 2001-08-21 Promega Corporation Deploymerization method for nucleic acid detection of an amplified nucleic acid target
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
US6270973B1 (en) 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
US6270974B1 (en) 1998-03-13 2001-08-07 Promega Corporation Exogenous nucleic acid detection
US7090975B2 (en) 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
US6361942B1 (en) 1998-03-24 2002-03-26 Boston Probes, Inc. Method, kits and compositions pertaining to detection complexes
US6255050B1 (en) 1998-05-22 2001-07-03 Lorne Park Research, Inc. Dynamic hybridization system
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
DE69941333D1 (de) 1998-07-02 2009-10-08 Gen Probe Inc Molekulare fackeln
GB9819418D0 (en) 1998-09-07 1998-10-28 Secr Defence Amplification method
US6153411A (en) * 1998-10-30 2000-11-28 American Water Works Company, Inc. Methods and kits for detection of Cryptosporidium parvum using immunomagnetic separation and amplification
US7141417B1 (en) 1999-02-25 2006-11-28 Thomas Jefferson University Compositions, kits, and methods relating to the human FEZ1 gene, a novel tumor suppressor gene
US6221635B1 (en) 1999-05-06 2001-04-24 The Wistar Institute Methods for solid-phase amplification of DNA template (SPADT) using multiarrays
US6645733B1 (en) 1999-06-25 2003-11-11 Ingeneus Corporation Fluorescent intensity method for assaying binding between proteins or peptides
US6692834B1 (en) * 1999-06-28 2004-02-17 Medtronic, Inc. Method for coating implantable devices
EP1215498A4 (de) * 1999-09-22 2005-04-20 Wakunaga Pharma Co Ltd Selbsterkennende hybridisierungssonde
WO2001038585A2 (en) 1999-11-24 2001-05-31 The Regents Of The University Of California Polymer arrays and methods of using labeled probe molecules to identify and quantify target molecule expression
GB2359625B (en) * 1999-12-10 2004-10-20 Molecular Light Tech Res Ltd Monitoring oligonucleotide binding process using chemiluminescence quenching
WO2006053770A1 (en) 2004-11-18 2006-05-26 Eppendorf Array Technologies Real-time pcr of targets on a micro-array
DE60140804D1 (de) * 2000-06-27 2010-01-28 Nat Inst Of Advanced Ind Scien Neue nukleinsäuresonden und verfahren zum testen von nukleinsäuren unter deren verwendung
JP5213297B2 (ja) 2000-08-11 2013-06-19 ユニバーシティ オブ ユタ リサーチ ファウンデーション 単一ラベルオリゴヌクレオチド・プローブ
CN1348096A (zh) 2000-10-10 2002-05-08 栾国彦 一种均相特异性检测核酸的探针及应用方法
JP2002125688A (ja) * 2000-10-30 2002-05-08 Tosoh Corp Ｃ型肝炎ウイルスの高感度検出のためのオリゴヌクレオチドおよび検出法
JP2002360257A (ja) * 2001-06-05 2002-12-17 Fuji Photo Film Co Ltd Ｄｎａマイクロアレイにおける遺伝子の検出法
WO2003043402A2 (en) * 2001-10-19 2003-05-30 Proligo Llc Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes
US6946245B2 (en) * 2001-12-04 2005-09-20 Quest Diagnostics Investments Incorporated Oligonucleotides and methods for detecting hepatitis C viral nucleic acids
US6818420B2 (en) 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
JP4481656B2 (ja) * 2002-02-27 2010-06-16 ライフ テクノロジーズ コーポレーション ３’→５’エキソヌクレアーゼ耐性クエンチャードメインを含むｆｅｔ標識オリゴヌクレオチドを使用する方法およびそれを実施するための組成物
ES2253461T3 (es) * 2002-03-05 2006-06-01 Epigenomics Ag Procedimiento y dispositivo para la determinacion de la especificidad del tejido y del adn que flota libre en tejidos corporales.
US7445893B2 (en) * 2002-04-12 2008-11-04 Primera Biosystems, Inc. Sampling method for amplification reaction analysis
US7081339B2 (en) * 2002-04-12 2006-07-25 Primera Biosystems, Inc. Methods for variation detection
JP4457001B2 (ja) * 2002-05-31 2010-04-28 セクレタリー・デパートメント・オブ・アトミック・エナジー ドナー／アクセプター部分が相補鎖上となる標的核酸検出のｍｅｔ／ｆｒｅｔに基づく方法
WO2004010101A2 (en) * 2002-07-18 2004-01-29 The Board Of Trustees Of The Leland Stanford Junior University Detection of chemical ligation using fluorescence quenching leaving groups
US7452712B2 (en) 2002-07-30 2008-11-18 Applied Biosystems Inc. Sample block apparatus and method of maintaining a microcard on a sample block
US7364848B2 (en) 2002-09-02 2008-04-29 Pamgene B.V. Integrated microarray analysis
US20040067492A1 (en) * 2002-10-04 2004-04-08 Allan Peng Reverse transcription on microarrays
US20050089875A1 (en) * 2002-11-21 2005-04-28 Sention Inc. Quantitative analysis of expression profiling information produced at various stages of an amplification process
EP3000899A1 (de) 2002-12-04 2016-03-30 Applied Biosystems, LLC Multiplexverstärkung von polynucleotiden
US20040259116A1 (en) * 2003-01-28 2004-12-23 Gorilla Genomics, Inc. Hairpin primer amplification
US20040219533A1 (en) * 2003-04-29 2004-11-04 Jim Davis Biological bar code
WO2004098386A2 (en) * 2003-05-01 2004-11-18 Gen-Probe Incorporated Oligonucleotides comprising a molecular switch
US20060024690A1 (en) * 2003-09-19 2006-02-02 Kao H P Normalization of data using controls
WO2005029041A2 (en) * 2003-09-19 2005-03-31 Applera Corporation High density sequence detection methods and apparatus
WO2005049849A2 (en) 2003-11-14 2005-06-02 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
WO2005051967A2 (en) * 2003-11-19 2005-06-09 Allelogic Biosciences Corp. Oligonucleotides labeled with a plurality of fluorophores
WO2005063992A1 (ja) * 2003-12-30 2005-07-14 Suntory Limited 新規血清型のストレプトコッカス ミュータンスおよびその利用
WO2005077125A2 (en) * 2004-02-11 2005-08-25 Applera Corporation Methods and compositions for detecting nucleic acids
EP1721011A1 (de) 2004-03-01 2006-11-15 Applera Corporation Verfahren, zusammensetzungen und kits zur verwendung bei der polynukleotidamplifikation
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
WO2005113773A1 (en) * 2004-05-20 2005-12-01 Warnex Research Inc. Polynucleotides for the detection of escherichia coli 0157:h7 and escherichia coli 0157:nm verotoxin producers
US20060003337A1 (en) * 2004-06-30 2006-01-05 John Brandis Detection of small RNAS
EP2093297A3 (de) 2004-09-07 2009-11-18 Telethon Institute for Child Health Research Wirkstoffe zur Behandlung oder Vorbeugung einer allergischen Störung
US20090068650A1 (en) * 2005-02-11 2009-03-12 Southern Illinois University Metabolic Primers for the Detection of (Per) Chlorate-Reducing Bacteria and Methods of Use Thereof
EP1853732A2 (de) * 2005-02-28 2007-11-14 Gen-Probe Incorporated Präparate und verfahren zur probenmessung durch eine umschaltbare nukleinsäuren-sonde
US8263330B1 (en) 2005-03-08 2012-09-11 Quest Diagnostics Investments Incorporated Detection of Mycobacterium tuberculosis complex nucleic acids
CA2842232C (en) 2005-03-10 2015-01-27 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
US7601498B2 (en) * 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
US7776567B2 (en) 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
WO2006127507A2 (en) * 2005-05-20 2006-11-30 Integrated Dna Technologies, Inc. Compounds and methods for labeling oligonucleotides
GB0514889D0 (en) 2005-07-20 2005-08-24 Lgc Ltd Oligonucleotides
US8017360B2 (en) * 2005-11-10 2011-09-13 Panomics, Inc. Detection of nucleic acids through amplification of surrogate nucleic acids
EP2025762A3 (de) 2006-01-17 2009-09-30 Health Research Inc. Heteroduplex-Trackingassay
US7824858B2 (en) 2006-01-23 2010-11-02 Quest Diagnostics Investments Incorporated Assay for mycobacterium avium/intracellulare nucleic acid
CA2645136C (en) * 2006-03-16 2017-09-19 Pentabase Aps Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids
EP2759307B1 (de) 2006-03-29 2016-11-09 Merial Limited Impfstoff gegen Streptokokken
US8078264B2 (en) 2006-07-11 2011-12-13 Case Western Reserve University Intra-operative molecular imaging
US7700756B2 (en) * 2006-07-27 2010-04-20 Southern Illinois University Metabolic primers for the detection of perchlorate-reducing bacteria and methods of use thereof
WO2008021446A2 (en) * 2006-08-15 2008-02-21 Genetag Technology, Inc. Probe-antiprobe compositions and methods for dna or rna detection
BRPI0718050A2 (pt) * 2006-11-02 2013-11-05 Veridex Llc Formação de imegens do endotélio vascular ativado usando agentes de contraste de mri imunomagnéticos
CN101595231A (zh) * 2006-12-29 2009-12-02 霍尼韦尔国际公司 用于微阵列的反应缓冲液
US9271653B2 (en) 2007-06-12 2016-03-01 Case Western Reserve University Intra-operative molecular imaging
DE102007029772B4 (de) 2007-06-22 2011-12-08 Aj Innuscreen Gmbh Verfahren und Schnelltest zum Nachweis spezifischer Nukleinsäuresequenzen
IL184478A (en) * 2007-07-08 2017-07-31 Hadasit Medical Res Services & Development Ltd Preparations, methods and kits for identifying carriers of mutations in genes 1brca and 2brca and for the early detection of cancers associated with mutations in these genes
EP2107125A1 (de) 2008-03-31 2009-10-07 Eppendorf Array Technologies SA (EAT) Echtzeit-PCR von Targets auf einem Mikroarray
US20100143901A1 (en) * 2008-12-09 2010-06-10 Roche Molecular Systems, Inc. Nuclease-Free Real-Time Detection of Nucleic Acids
US8691504B2 (en) 2009-05-26 2014-04-08 Xiamen University Method for detecting variations in nucleic acid sequences
WO2011054644A1 (en) 2009-10-14 2011-05-12 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. A low density lipoprotein-related protein 1 splice variant as cancer marker
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
US9506057B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds
AU2011230496B2 (en) 2010-03-26 2015-09-17 Integrated Dna Technologies, Inc. Methods for enhancing nucleic acid hybridization
DE102010003782B4 (de) 2010-04-08 2023-09-28 Ist Innuscreen Gmbh Vorrichtung zum Nachweis von Nukleinsäuren
US9234249B2 (en) 2010-07-12 2016-01-12 Gen-Probe Incorporated Compositions and assays to detect swine H1N1 influenza A virus, seasonal H1 influenza A virus and seasonal H3 influenza A virus nucleic acids
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
WO2012016936A1 (de) 2010-07-31 2012-02-09 Aj Innuscreen Gmbh Nachweis spezifischer nukleinsäuresequenzen mittels fluoreszenz - quenching
DE102010033107A1 (de) 2010-08-02 2012-02-02 Aj Innuscreen Gmbh Nachweis spezifischer Nukleinsäuresequenzen mittels Fluoreszenzlöschung
WO2012033848A1 (en) 2010-09-07 2012-03-15 Integrated Dna Technologies, Inc. Modifications for antisense compounds
US9051606B2 (en) 2010-09-10 2015-06-09 Qiagen Gaithersburg, Inc. Methods and compositions for nucleic acid detection
CA2812512A1 (en) 2010-09-29 2012-04-05 Mor Research Applications Ltd. Prognostic methods, compositions and kits for prediction of acute lymphoblastic leukemia (all) relapse
CN103403533B (zh) 2011-02-24 2017-02-15 简.探针公司 用于分辨光信号检测器中不同调制频率的光信号的系统和方法
DE102011078342A1 (de) 2011-06-29 2013-01-03 Aj Innuscreen Gmbh Rehybridisierendes Sondensystem zur qualitativen und quantitativen Messung von spezifischen Nukleinsäuren in Echtzeit
WO2013030841A1 (en) 2011-09-04 2013-03-07 Yissum Research Development Company Of The Hebrew Universitiy Of Jerusalem Ltd. Prognostic methods and compositions for predicting interferon treatment eficacy in a subject
EP4361606A2 (de) 2012-02-03 2024-05-01 California Institute of Technology Signalkodierung und -dekodierung in multiplexierten biochemischen assays
EP2820157B1 (de) 2012-03-02 2019-05-01 Winthrop-University Hospital Verfahren zur verwendung von sondenbasierter pcr-detektion zum messen der konzentration von dna aus zirkulierenden entmethylierten beta-zellen als massstab für beta-zell-verlust bei diabetes
US9133509B2 (en) 2012-03-22 2015-09-15 Lgc Genomics Limited Polymerase chain reaction detection system
WO2013168162A1 (en) 2012-05-09 2013-11-14 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Clustered single nucleotide polymorphisms in the human acetylcholinesterase gene and uses thereof in diagnosis and therapy
EP3901243A1 (de) 2012-08-03 2021-10-27 California Institute of Technology Multiplexing und quantifizierung in pcr mit reduzierter hardware und reduzierten anforderungen
PL2712790T3 (pl) 2012-09-26 2016-03-31 3A Composites Gmbh Wypukły dach pojazdu z elementami wzmacniającymi i tłumiącymi
CN103114131B (zh) 2012-11-30 2018-10-02 珠海市坤元农业科技有限公司 一种引物中部序列干扰pcr技术
AU2013202805B2 (en) 2013-03-14 2015-07-16 Gen-Probe Incorporated System and method for extending the capabilities of a diagnostic analyzer
US20160265058A1 (en) 2013-11-01 2016-09-15 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Diagnostic methods and kits for determining a personalized treatment regimen for a subject suffering from a pathologic disorder
ES2703792T3 (es) 2013-11-22 2019-03-12 Orion Diagnostica Oy Detección de ácidos nucleicos mediante amplificación basada en invasión de hebra
EP2933341B1 (de) 2014-04-18 2017-10-04 Schweitzer Biotech Company Ltd. Verfahren zum nachweis von krankheitserregern in kaltwasserfisch
GB201410022D0 (en) 2014-06-05 2014-07-16 Orion Diagnostica Oy Method
EP3175001B1 (de) 2014-07-30 2021-02-10 Mor Research Applications Ltd. Prognoseverfahren und systeme zur behandlung von akuter lymphoblastischer leukämie
US9856521B2 (en) * 2015-01-27 2018-01-02 BioSpyder Technologies, Inc. Ligation assays in liquid phase
US10683534B2 (en) * 2015-01-27 2020-06-16 BioSpyder Technologies, Inc. Ligation assays in liquid phase
US11091810B2 (en) * 2015-01-27 2021-08-17 BioSpyder Technologies, Inc. Focal gene expression profiling of stained FFPE tissues with spatial correlation to morphology
EP3359690A4 (de) 2015-10-09 2019-06-26 Genefron Ltd. Verfahren und kits zur vorhersage und diagnose der kongenitalen übertragung des humanen cytomegalovirus (hcmv)
WO2017218777A1 (en) 2016-06-17 2017-12-21 California Institute Of Technology Nucleic acid reactions and related methods and compositions
JP6853523B2 (ja) * 2016-07-27 2021-03-31 国立大学法人京都大学 ヘリカーゼを用いたｐｃｒ
CN111004864A (zh) 2018-10-05 2020-04-14 福又达生物科技股份有限公司 虾病原体检测方法
IT202100009338A1 (it) 2021-04-14 2022-10-14 Celera Digital S R L Sistema di interconnessione e predizione aziendale e metodo di funzionamento di sistema di segnalazione
DK202200230A1 (en) 2022-03-21 2023-12-11 Samplix Aps Targeted enrichment of large dna molecules for long-read sequencing using facs or microfluidic partitioning

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4220450A (en) 1978-04-05 1980-09-02 Syva Company Chemically induced fluorescence immunoassay
US4766064A (en) 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay employing polyether and diagnostic kit
CA1273552A (en) * 1985-12-23 1990-09-04 Michael J. Heller Fluorescent stokes shift probes for polynucleotide hybridization assays
ATE88761T1 (de) 1986-01-10 1993-05-15 Amoco Corp Kompetitiver homogener test.
US5635347A (en) 1986-04-30 1997-06-03 Igen, Inc. Rapid assays for amplification products
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5273879A (en) * 1987-07-23 1993-12-28 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
WO1990003445A1 (en) 1988-09-30 1990-04-05 The Salk Institute For Biological Studies Target nucleic acid amplification/detection systems
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
WO1992014845A1 (en) * 1991-02-26 1992-09-03 Worcester Foundation For Experimental Biology Diagnosing cystic fibrosis and other genetic diseases using fluorescence resonance energy transfer (fret)
DK0519338T3 (da) * 1991-06-20 1996-10-28 Hoffmann La Roche Forbedrede fremgangsmåder til nukleinsyreamplifikation
CA2218875C (en) 1991-07-23 2000-11-07 The Research Foundation Of State University Of New York Improvements in the in situ pcr
CA2080305A1 (en) 1991-10-11 1993-04-12 Linda M. Western Method for producing a polynucleotide for use in single primer amplification and phosphorothioate-containing oligonucleotides as primers in nucleic acid amplification
WO1993010267A1 (en) * 1991-11-15 1993-05-27 Igen, Inc. Rapid assays for amplification products
US5332659A (en) 1992-04-09 1994-07-26 The United States Of America As Represented By The Secretary Of The Navy Light emission-or absorbance-based binding assays for polynucleic acids
US6020124A (en) 1992-04-27 2000-02-01 Trustees Of Dartmouth College Detection of soluble gene sequences in biological fluids
GB9211979D0 (en) 1992-06-05 1992-07-15 Buchard Ole Uses of nucleic acid analogues
US5849544A (en) 1992-07-24 1998-12-15 University Of Australia Amplification and detection process
WO1994003472A1 (en) * 1992-08-04 1994-02-17 Gen-Probe Incorporated Nucleic acid sequence amplification
EP0599388B1 (de) 1992-11-20 2000-08-02 Koninklijke Philips Electronics N.V. Halbleitervorrichtung mit einem programmierbaren Element
JPH06153997A (ja) 1992-11-27 1994-06-03 Canon Inc 検出信号増幅による標的核酸の検出方法
EP0601889A2 (de) * 1992-12-10 1994-06-15 Maine Medical Center Research Institute Nukleinsäure-Sonden
US5364790A (en) 1993-02-16 1994-11-15 The Perkin-Elmer Corporation In situ PCR amplification system
US5422252A (en) * 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
GB9311386D0 (en) 1993-06-02 1993-07-21 Pna Diagnostics As Nucleic acid analogue assay procedures
DE69422678T2 (de) * 1993-10-12 2001-02-22 Matsushita Electric Ind Co Ltd Verschlüsselungssystem, Verschlüsselungsgerät und Entschlüsselungsgerät
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
JP4071277B2 (ja) 1993-11-12 2008-04-02 ピーエイチアールアイ・プロパティーズ・インコーポレーテッド 核酸検出用ハイブリダイゼーションプローブ、共通ステム、方法およびキット
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5629178A (en) 1994-10-28 1997-05-13 Genetics & Ivf Institute Method for enhancing amplification in the polymerase chain reaction employing peptide nucleic acid (PNA)
IE72524B1 (en) 1994-11-04 1997-04-23 Elan Med Tech Analyte-controlled liquid delivery device and analyte monitor
WO1996034983A1 (en) 1995-05-05 1996-11-07 The Perkin-Elmer Corporation Methods and reagents for combined pcr amplification and hybridization probing assay
EP0845047A2 (de) 1995-08-14 1998-06-03 Abbott Laboratories Eintopf-amplifikationstestverfahren für nukleinsäuren

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102010003781A1 (de) * 2010-04-08 2011-12-15 Aj Innuscreen Gmbh Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen
DE102010003781B4 (de) * 2010-04-08 2012-08-16 Aj Innuscreen Gmbh Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen

Also Published As

Publication number Publication date
AU5442896A (en) 1996-11-21
DE69610304D1 (de) 2000-10-19
CA2219891C (en) 2002-01-29
US6395518B1 (en) 2002-05-28
JP2004000185A (ja) 2004-01-08
US7847076B2 (en) 2010-12-07
CA2219891A1 (en) 1996-11-07
JPH11504219A (ja) 1999-04-20
JP4040087B2 (ja) 2008-01-30
US7241596B2 (en) 2007-07-10
US20110275071A1 (en) 2011-11-10
US20090258351A1 (en) 2009-10-15
US7413708B2 (en) 2008-08-19
ATE196322T1 (de) 2000-09-15
JP2005110666A (ja) 2005-04-28
WO1996034983A1 (en) 1996-11-07
US6485903B1 (en) 2002-11-26
EP0826066B1 (de) 2000-09-13
EP0826066A1 (de) 1998-03-04
US20030215826A1 (en) 2003-11-20
US20050227247A1 (en) 2005-10-13
US5691146A (en) 1997-11-25
AU693023B2 (en) 1998-06-18
JP4040519B2 (ja) 2008-01-30
US20080050742A1 (en) 2008-02-28

Similar Documents

Publication Publication Date Title
DE69610304D1 (de) Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
JP2870696B2 (ja) Ｄｎａ配列決定
DE69838210T2 (de) Markierter Primer, geeignet für die Detektion von Nukleinsäuren
US5885775A (en) Methods for determining sequences information in polynucleotides using mass spectrometry
US5364759B1 (en) Dna typing with short tandem repeat polymorphisms and indentification of polymorphic short tandem repeats
DE69034177D1 (de) Verfahren zur Amplifikation von Nukleinsäuresequenzen
CA2308599A1 (en) Dna polymorphism identity determination using flow cytometry
ATE398187T1 (de) Verfahren zum nachweis von ziel-nukleinsäuren mittels pcr
ATE185843T1 (de) Nukleinsäure-sequenzanalyse durch die methode der parallelen primerextension
WO2002008414A1 (fr) Nouvelles sondes d'acides nucléiques et procédés d'essai d'acide nucléique par utilisation des mêmes
WO1993016094A3 (en) Applications of fluorescent n-nucleosides and fluorescent structural analogs of n-nucleosides
KR970028548A (ko) 핵산의 형광 편광도 검출방법
DE69417863T2 (de) Verbessertes verfahren für die quantifizierung von nukleinsäuren
DE60113945D1 (de) Verfahren zur Analyse von Wiederkehrenden PCR-Podukten, das auf der Analyse von DNS-Schmelzkurven basiert
EP0741799B1 (de) Methode zur sequenzierung kurzer oligonukleotide
ATE286144T1 (de) VERWENDUNG VON FLUORESZIERENDEN ßMOLECULAR BEACONSß ZUM NACHWEIS VON METHYLIERTEN NUKLEINSÄUREN
DE60133534T2 (de) Ein homogenes verfahren zur detektion eines polynukleotids, mit beispielsweise abspaltbarer lanthanidchelat markierung
WO2002088379A3 (en) Methods and compositions for utilizing changes of hybridization signals during approach to equilibrium
Basagoudanavar et al. Random amplification of polymorphic DNA fingerprinting of Trypanosoma evansi
EP1241267A3 (de) Enzym-Aktivitätstest mittels fluoreszenzmarkierter Oligonukleotid-Substrate
DE19757300A1 (de) Nachweis von Harnblasenkarzinom in einer Urinprobe
WO2001009376A1 (en) Method for amplification of nucleic acids
JP2005052002A (ja) 非環状核酸断片の増幅方法
WO1996027023A2 (en) Method of using mobile priming sites for dna sequencing
JPH03164200A (ja) 液体ハイブリダイゼーションによる遺伝子の分析方法

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: APPLERA CORP., FOSTER CITY, CALIF., US

8327 Change in the person/name/address of the patent owner

Owner name: APPLIED BIOSYSTEMS, LLC (N. D. GES. D. STAATES, US

8380 Miscellaneous part iii

Free format text: PFANDRECHT

8380 Miscellaneous part iii

Free format text: PFANDRECHT AUFGEHOBEN