DE69314951T2 - Dns sequenzierungsverfahren - Google Patents

Dns sequenzierungsverfahren

Info

Publication number
DE69314951T2
DE69314951T2 DE69314951T DE69314951T DE69314951T2 DE 69314951 T2 DE69314951 T2 DE 69314951T2 DE 69314951 T DE69314951 T DE 69314951T DE 69314951 T DE69314951 T DE 69314951T DE 69314951 T2 DE69314951 T2 DE 69314951T2
Authority
DE
Germany
Prior art keywords
pct
dns
sequencing procedure
sec
date
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69314951T
Other languages
English (en)
Other versions
DE69314951D1 (de
Inventor
Andre Rosenthal
Sydney Brenner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of DE69314951D1 publication Critical patent/DE69314951D1/de
Application granted granted Critical
Publication of DE69314951T2 publication Critical patent/DE69314951T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
DE69314951T 1992-04-22 1993-04-22 Dns sequenzierungsverfahren Expired - Lifetime DE69314951T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB929208733A GB9208733D0 (en) 1992-04-22 1992-04-22 Dna sequencing method
PCT/GB1993/000848 WO1993021340A1 (en) 1992-04-22 1993-04-22 Dna sequencing method

Publications (2)

Publication Number Publication Date
DE69314951D1 DE69314951D1 (de) 1997-12-04
DE69314951T2 true DE69314951T2 (de) 1998-03-19

Family

ID=10714401

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69314951T Expired - Lifetime DE69314951T2 (de) 1992-04-22 1993-04-22 Dns sequenzierungsverfahren

Country Status (10)

Country Link
US (1) US6087095A (de)
EP (1) EP0640146B1 (de)
JP (1) JPH07507681A (de)
AT (1) ATE159766T1 (de)
AU (1) AU4020893A (de)
CA (1) CA2133956C (de)
DE (1) DE69314951T2 (de)
ES (1) ES2110604T3 (de)
GB (1) GB9208733D0 (de)
WO (1) WO1993021340A1 (de)

Families Citing this family (205)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6153379A (en) * 1993-06-22 2000-11-28 Baylor College Of Medicine Parallel primer extension approach to nucleic acid sequence analysis
US7001722B1 (en) 1993-06-22 2006-02-21 Baylor College Of Medicine Parallel primer extension approach to nucleic acid sequence analysis
EP0663447B1 (de) * 1993-12-28 2003-07-09 Eiken Chemical Co., Ltd. Verfahren für den Nachweis spezifischer Polynukleotiden
US5552278A (en) * 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
FR2718753B1 (fr) * 1994-04-15 1996-07-19 Pasteur Institut Procédé de comptage de mono-, di- et trinucléotides répétés dans un génome eucaryote et trousse permettant la mise en Óoeuvre de ce procédé.
US5986076A (en) * 1994-05-11 1999-11-16 Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
US6358689B1 (en) * 1994-05-11 2002-03-19 Boston University Detection of markers in nascent proteins
US6589736B1 (en) 1994-11-22 2003-07-08 The Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
USRE43097E1 (en) 1994-10-13 2012-01-10 Illumina, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US6001571A (en) 1995-11-30 1999-12-14 Mandecki; Wlodek Multiplex assay for nucleic acids employing transponders
US6051377A (en) * 1995-11-30 2000-04-18 Pharmaseq, Inc. Multiplex assay for nucleic acids employing transponders
US5736332A (en) * 1995-11-30 1998-04-07 Mandecki; Wlodek Method of determining the sequence of nucleic acids employing solid-phase particles carrying transponders
AU1061997A (en) 1995-11-30 1997-06-19 Wlodek Mandecki Screening of drugs from chemical combinatorial libraries employing transponders
US5641634A (en) 1995-11-30 1997-06-24 Mandecki; Wlodek Electronically-indexed solid-phase assay for biomolecules
ES2144846T5 (es) * 1996-01-23 2006-07-16 Qiagen Genomics, Inc. Metodos y composiciones para determinar la secuencia de moleculas de acidos nucleicos.
EP0962464A3 (de) * 1996-01-23 2004-02-11 Qiagen Genomics, Inc. Methoden und Zusammensetzungen zum Nachweis der Bindung eines Liganden-Paars mittels nicht-fluoriszierender Markierungen
US6027890A (en) 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
US6613508B1 (en) * 1996-01-23 2003-09-02 Qiagen Genomics, Inc. Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques
US6312893B1 (en) 1996-01-23 2001-11-06 Qiagen Genomics, Inc. Methods and compositions for determining the sequence of nucleic acid molecules
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
US5858671A (en) 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6258533B1 (en) 1996-11-01 2001-07-10 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
CA2281205A1 (en) 1997-02-12 1998-08-13 Eugene Y. Chan Methods and products for analyzing polymers
US6197557B1 (en) 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US6117634A (en) * 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US20030027126A1 (en) 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
AU6846798A (en) 1997-04-01 1998-10-22 Glaxo Group Limited Method of nucleic acid sequencing
EP3034626A1 (de) 1997-04-01 2016-06-22 Illumina Cambridge Limited Verfahren zur vervielfältigung von nukleinsäuren
US5981166A (en) * 1997-04-23 1999-11-09 Pharmaseq, Inc. Screening of soluble chemical compounds for their pharmacological properties utilizing transponders
EP0985142A4 (de) * 1997-05-23 2006-09-13 Lynx Therapeutics Inc System und gerät zur sequentiellen behandlung von analyten
PT1017848E (pt) * 1997-07-28 2003-02-28 Medical Biosystems Ltd Analise de sequencias de acido nucleico
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
JP3813818B2 (ja) * 1998-05-01 2006-08-23 アリゾナ ボード オブ リージェンツ オリゴヌクレオチドおよびｄｎａ分子のヌクレオチド配列の決定方法
US20030022207A1 (en) * 1998-10-16 2003-01-30 Solexa, Ltd. Arrayed polynucleotides and their use in genome analysis
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US20040106110A1 (en) * 1998-07-30 2004-06-03 Solexa, Ltd. Preparation of polynucleotide arrays
US20100130368A1 (en) * 1998-07-30 2010-05-27 Shankar Balasubramanian Method and system for sequencing polynucleotides
US7270958B2 (en) 1998-09-10 2007-09-18 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US7479472B1 (en) * 1998-10-19 2009-01-20 The Board Of Trustees Of The Leland Stanford Junior University DNA-templated combinatorial library chemistry
US6221592B1 (en) 1998-10-20 2001-04-24 Wisconsin Alumi Research Foundation Computer-based methods and systems for sequencing of individual nucleic acid molecules
US6607888B2 (en) 1998-10-20 2003-08-19 Wisconsin Alumni Research Foundation Method for analyzing nucleic acid reactions
CN1331700A (zh) 1998-11-16 2002-01-16 基因纬生物技术公司 通过禽类中使用多核苷酸疫苗接种生成抗体的方法
CA2355816C (en) 1998-12-14 2007-10-30 Li-Cor, Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
AU3372800A (en) 1999-02-23 2000-09-14 Caliper Technologies Corporation Manipulation of microparticles in microfluidic systems
AU3174600A (en) * 1999-03-10 2000-09-28 Asm Scientific, Inc. A method for direct nucleic acid sequencing
WO2000058507A1 (en) * 1999-03-30 2000-10-05 Solexa Ltd. Polynucleotide sequencing
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
EP1681357A3 (de) * 1999-05-19 2006-07-26 Cornell Research Foundation, Inc. Methode zur Nukleinsäuresequenzierung
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
WO2001023610A2 (en) * 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
GB9923644D0 (en) 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
US6908736B1 (en) 1999-10-06 2005-06-21 Medical Biosystems, Ltd. DNA sequencing method
US7332275B2 (en) 1999-10-13 2008-02-19 Sequenom, Inc. Methods for detecting methylated nucleotides
EP1228244A4 (de) * 1999-11-04 2005-02-09 California Inst Of Techn Verfahren und apparate zur analyse von polynukleotidsequenzen
WO2001073121A1 (fr) 2000-03-30 2001-10-04 Toyota Jidosha Kabushiki Kaisha Procede pour determiner une sequence de base d'une molecule individuelle d'acide nucleique
GB0016472D0 (en) * 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method and apparatus
US6994963B1 (en) * 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
GB0021286D0 (en) * 2000-08-30 2000-10-18 Gemini Genomics Ab Identification of drug metabolic capacity
EP3034627B1 (de) 2000-10-06 2019-01-30 The Trustees of Columbia University in the City of New York Massives paralleles verfahren zum entschlüsseln von dna und rna
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
DE60222628T2 (de) * 2001-01-30 2008-07-17 Solexa Ltd., Saffron Walden Herstellung von matrizen aus polynukleotiden
WO2002072892A1 (en) * 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
DE10120797B4 (de) * 2001-04-27 2005-12-22 Genovoxx Gmbh Verfahren zur Analyse von Nukleinsäureketten
US6613523B2 (en) * 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
AU2002337030A1 (en) * 2001-08-29 2003-03-18 Genovoxx Gmbh Method for analyzing nucleic acid sequences and gene expression
US20050123913A1 (en) * 2001-08-30 2005-06-09 Emory University Human mitochondrial dna polymorphisms, haplogroups, associations with physiological conditions, and genotyping arrays
WO2003054143A2 (en) * 2001-10-25 2003-07-03 Neurogenetics, Inc. Genes and polymorphisms on chromosome 10 associated with alzheimer's disease and other neurodegenerative diseases
US20030224380A1 (en) * 2001-10-25 2003-12-04 The General Hospital Corporation Genes and polymorphisms on chromosome 10 associated with Alzheimer's disease and other neurodegenerative diseases
US20030170678A1 (en) * 2001-10-25 2003-09-11 Neurogenetics, Inc. Genetic markers for Alzheimer's disease and methods using the same
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7223538B2 (en) * 2001-12-14 2007-05-29 Ge Healthcare Bio-Sciences Ab Post-synthesis labeling of nucleic acids, assays using nucleic acids that are labeled post-synthetically, single nucleotide polymorphism detection, and associated compounds and microarrays
US20030228611A1 (en) * 2002-05-01 2003-12-11 President And Fellows Of Harvard College Nucleic acid memory device
US20030215816A1 (en) * 2002-05-20 2003-11-20 Narayan Sundararajan Method for sequencing nucleic acids by observing the uptake of nucleotides modified with bulky groups
US20030220844A1 (en) * 2002-05-24 2003-11-27 Marnellos Georgios E. Method and system for purchasing genetic data
WO2003102216A2 (en) 2002-05-31 2003-12-11 Diversa Corporation Multiplexed systems for nucleic acid sequencing
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
EP1530578B1 (de) 2002-08-23 2013-03-13 Illumina Cambridge Limited Modifizierte nukleotide für polynukleotidsequencing
EP2112229A3 (de) 2002-11-25 2009-12-02 Sequenom, Inc. Verfahren zum Identifizieren des Brustkrebsrisikos und zugehörige Behandlungen
WO2004050916A1 (en) * 2002-12-02 2004-06-17 Solexa Limited Recovery of original template
ES2342665T3 (es) 2003-01-29 2010-07-12 454 Corporation Secuenciacion desde dos extremos.
WO2004072294A2 (en) * 2003-02-12 2004-08-26 Genizon Svenska Ab Methods and means for nucleic acid sequencing
GB0308852D0 (en) * 2003-04-16 2003-05-21 Lingvitae As Method
DE602004018631D1 (de) * 2003-04-24 2009-02-05 Afshin Ahmadian En
US20050053980A1 (en) * 2003-06-20 2005-03-10 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US20040259100A1 (en) * 2003-06-20 2004-12-23 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US20050181394A1 (en) * 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
GB0324456D0 (en) * 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
EP2436778A3 (de) * 2004-03-03 2012-07-11 The Trustees of Columbia University in the City of New York Photospaltbare fluoreszierende Nukleotide zur DNA-Sequenzierung auf einem durch ortsspezifische Kopplungschemie konstruierten Chip
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
EP1773860A4 (de) * 2004-07-22 2009-05-06 Sequenom Inc Verfahren zur identifizierung des risikos von typ-ii-diabetes und zur behandlung davon
GB2423819B (en) 2004-09-17 2008-02-06 Pacific Biosciences California Apparatus and method for analysis of molecules
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US20060172328A1 (en) * 2005-01-05 2006-08-03 Buzby Philip R Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction
WO2006083751A2 (en) * 2005-01-31 2006-08-10 Pacific Biosciences Of California, Inc. Use of reversible extension terminator in nucleic acid sequencing
EP2230315A1 (de) 2005-02-01 2010-09-22 AB Advanced Genetic Analysis Corporation Nukleinsäuresequenzierung durch schrittweise Duplexverlängerung
EP2233583A1 (de) 2005-02-01 2010-09-29 AB Advanced Genetic Analysis Corporation Nukleinsäuresequenzierung durch schrittweise Duplexverlängerung
US20060263790A1 (en) * 2005-05-20 2006-11-23 Timothy Harris Methods for improving fidelity in a nucleic acid synthesis reaction
WO2007002204A2 (en) * 2005-06-21 2007-01-04 The Trustees Of Columbia University In The City Of New York Pyrosequencing methods and related compostions
JP2008546424A (ja) * 2005-06-28 2008-12-25 アジェンコート パーソナル ジェノミクス コーポレイション 修飾ポリヌクレオチドを作製する方法および配列決定する方法
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
GB2446084B (en) 2005-10-31 2011-03-02 Univ Columbia Synthesis of four color 3-o-allyl modified photocleavable fluorescent nucleotides and related methods
US8796432B2 (en) 2005-10-31 2014-08-05 The Trustees Of Columbia University In The City Of New York Chemically cleavable 3'-o-allyl-DNTP-allyl-fluorophore fluorescent nucleotide analogues and related methods
US20090088332A1 (en) * 2005-11-21 2009-04-02 Jingyue Ju Multiplex Digital Immuno-Sensing Using a Library of Photocleavable Mass Tags
DE202006020290U1 (de) * 2006-03-13 2008-02-28 Schubert, Adrian Vorrichtung zur Erkennung und Identifizierung von Zielstrukturen
EP2029777B1 (de) 2006-05-31 2017-03-08 Sequenom, Inc. Verfahren und zusammensetzungen zur nukleinsäureextraktion aus einer probe
EP2035439A4 (de) 2006-06-05 2010-01-13 Cancer Care Ontario Abschätzung des risikos von colorectalkrebs
US8889348B2 (en) 2006-06-07 2014-11-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by nanopore using modified nucleotides
JP5244103B2 (ja) 2006-08-09 2013-07-24 ホームステッド クリニカル コーポレイション 器官特異的蛋白質およびその使用方法
US8399188B2 (en) 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
WO2008069973A2 (en) 2006-12-01 2008-06-12 The Trustees Of Columbia University In The City Of New York Four-color dna sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
WO2008098142A2 (en) 2007-02-08 2008-08-14 Sequenom, Inc. Nucleic acid-based tests for rhd typing, gender determination and nucleic acid quantification
AU2008222563A1 (en) 2007-03-05 2008-09-12 Cancer Care Ontario Assessment of risk for colorectal cancer
US8652780B2 (en) 2007-03-26 2014-02-18 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
WO2009032781A2 (en) 2007-08-29 2009-03-12 Sequenom, Inc. Methods and compositions for universal size-specific polymerase chain reaction
US20110014611A1 (en) * 2007-10-19 2011-01-20 Jingyue Ju Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequences by synthesis
US9115163B2 (en) 2007-10-19 2015-08-25 The Trustees Of Columbia University In The City Of New York DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
US8017338B2 (en) * 2007-11-20 2011-09-13 Life Technologies Corporation Reversible di-nucleotide terminator sequencing
US20090171078A1 (en) * 2007-11-20 2009-07-02 Applied Biosystems Inc. Method of sequencing nucleic acids using elaborated nucleotide phosphorotiolate compounds
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
GB0804024D0 (en) * 2008-03-04 2008-04-09 Mir Kalim U Multiplex selection
AU2009223671B2 (en) 2008-03-11 2014-11-27 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
CA2718137A1 (en) 2008-03-26 2009-10-01 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
BRPI0909212A2 (pt) 2008-03-28 2015-08-18 Pacific Biosciences California Composições e método para sequeciamento de ácido nucléico
CA2727709C (en) 2008-06-11 2017-11-14 Lasergen, Inc. Nucleotides and nucleosides and methods for their use in dna sequencing
CN102177438A (zh) 2008-07-25 2011-09-07 理查德·W·瓦格纳 蛋白筛选方法
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
WO2010038042A1 (en) 2008-10-02 2010-04-08 Illumina Cambridge Ltd. Nucleic acid sample enrichment for sequencing applications
US8392126B2 (en) 2008-10-03 2013-03-05 Illumina, Inc. Method and system for determining the accuracy of DNA base identifications
US8993230B2 (en) 2008-12-04 2015-03-31 Pacific Biosciences of Californ, Inc. Asynchronous sequencing of biological polymers
JP2012517238A (ja) 2009-02-11 2012-08-02 カリス エムピーアイ インコーポレイテッド 腫瘍の分子プロファイリング法
EP2408936A4 (de) * 2009-03-18 2013-01-30 Sequenom Inc Verwendung wärmestabiler endonukleasen zur herstellung von reportermolekülen
WO2010111686A2 (en) 2009-03-27 2010-09-30 Life Technologies Corp Labeled enzyme compositions, methods & systems
WO2010117804A2 (en) * 2009-03-30 2010-10-14 The Regents Of The University Of California Mostly natural dna sequencing by synthesis
EP3211095B1 (de) 2009-04-03 2019-01-02 Sequenom, Inc. Zusammensetzungen und verfahren für die herstellung von nukleinsäuren
US8182994B2 (en) 2009-09-15 2012-05-22 Illumina Cambridge Limited Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing
WO2011056688A2 (en) 2009-10-27 2011-05-12 Caris Life Sciences, Inc. Molecular profiling for personalized medicine
ES2577017T3 (es) 2009-12-22 2016-07-12 Sequenom, Inc. Procedimientos y kits para identificar la aneuploidia
US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
EP3327140A1 (de) 2010-09-16 2018-05-30 Gen-Probe Incorporated Über l-nukleotidenden immobilisierbare fängersonden
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
NZ610129A (en) 2010-10-04 2014-08-29 Genapsys Inc Systems and methods for automated reusable parallel biological reactions
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9255293B2 (en) 2010-11-01 2016-02-09 Gen-Probe Incorporated Integrated capture and amplification of target nucleic acid for sequencing
US9121059B2 (en) 2010-12-22 2015-09-01 Genia Technologies, Inc. Nanopore-based single molecule characterization
US9581563B2 (en) 2011-01-24 2017-02-28 Genia Technologies, Inc. System for communicating information from an array of sensors
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
AU2012249531B2 (en) 2011-04-29 2017-06-29 Sequenom, Inc. Quantification of a minority nucleic acid species
US9624539B2 (en) 2011-05-23 2017-04-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by synthesis using Raman and infrared spectroscopy detection
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US8889860B2 (en) 2011-09-13 2014-11-18 Lasergen, Inc. 3′-OH unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
CN104105797B (zh) * 2011-12-01 2016-08-31 吉纳普赛斯股份有限公司 用于高效电子测序与检测的系统和方法
CA2863084C (en) 2012-02-01 2020-07-07 Gen-Probe Incorporated Asymmetric hairpin target capture oligomers
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
EP2820129A1 (de) 2012-03-02 2015-01-07 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
JP2015525077A (ja) 2012-06-15 2015-09-03 ジェニア・テクノロジーズ・インコーポレイテッド チップの構成および高精度な核酸配列決定
CA2878979C (en) 2012-07-13 2021-09-14 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
WO2014015098A1 (en) 2012-07-18 2014-01-23 Siemens Healthcare Diagnostics Inc. A method of normalizing biological samples
EP2912194B1 (de) 2012-10-26 2019-05-08 Memorial Sloan-Kettering Cancer Center Androgenrezeptorvarianten und verfahren zur herstellung und verwendung
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
US9896728B2 (en) 2013-01-29 2018-02-20 Arcticrx Ltd. Method for determining a therapeutic approach for the treatment of age-related macular degeneration (AMD)
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
US9650406B2 (en) 2013-02-28 2017-05-16 Centrillion Technology Holdings Corporation Reversible terminator molecules and methods of their use
US11060145B2 (en) 2013-03-13 2021-07-13 Sequenom, Inc. Methods and compositions for identifying presence or absence of hypermethylation or hypomethylation locus
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
EP3388442A1 (de) 2013-03-15 2018-10-17 Illumina Cambridge Limited Modifizierte nukleoside oder nukleotide
US9809852B2 (en) 2013-03-15 2017-11-07 Genapsys, Inc. Systems and methods for biological analysis
EP3008211A4 (de) 2013-06-11 2016-12-21 Courtagen Life Sciences Inc Verfahren und kits zur behandlung und klassifizierung von personen mit risiko einer oder bestehender trap1-funktionsänderung
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
US9322062B2 (en) 2013-10-23 2016-04-26 Genia Technologies, Inc. Process for biosensor well formation
CN109797199A (zh) 2013-10-23 2019-05-24 吉尼亚科技公司 使用纳米孔的高速分子感测
EP3792921A1 (de) 2013-12-11 2021-03-17 Genapsys, Inc. Systeme und verfahren für biologische analysen und berechnungen
EP3117011B1 (de) 2014-03-13 2020-05-06 Sequenom, Inc. Verfahren und prozesse zur nicht-invasiven beurteilung genetischer variationen
EP3132060B1 (de) 2014-04-18 2019-03-13 Genapsys Inc. Verfahren und systeme zur nukleinsäureamplifikation
WO2015167972A1 (en) * 2014-04-28 2015-11-05 Tsavachidou Dimitra Methods for nucleic acid base determination
EP3091026B1 (de) 2015-05-08 2019-02-20 Centrillion Technology Holdings Corporation Disulfidvernetzte reversible terminatoren
MA45455A (fr) 2016-06-27 2019-05-01 Juno Therapeutics Inc Procédé d'identification d'épitopes peptidiques, molécules qui se lient à de tels épitopes et utilisations associées
MA45491A (fr) 2016-06-27 2019-05-01 Juno Therapeutics Inc Épitopes à restriction cmh-e, molécules de liaison et procédés et utilisations associés
CN109790575A (zh) 2016-07-20 2019-05-21 吉纳普赛斯股份有限公司 用于核酸测序的系统和方法
CA3076378A1 (en) 2017-09-21 2019-03-28 Genapsys, Inc. Systems and methods for nucleic acid sequencing
WO2019089982A1 (en) 2017-11-01 2019-05-09 Juno Therapeutics, Inc. Method of assessing activity of recombinant antigen receptors
WO2020069424A1 (en) 2018-09-28 2020-04-02 Centrillion Technologies, Inc. Disulfide-linked reversible terminators
MX2021006234A (es) 2018-11-30 2021-09-10 Caris Mpi Inc Perfilado molecular de proxima generacion.
IL293489A (en) 2019-12-02 2022-08-01 Caris Mpi Inc predictor of pan-cancer platinum response

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4863849A (en) * 1985-07-18 1989-09-05 New York Medical College Automatable process for sequencing nucleotide
US4962037A (en) * 1987-10-07 1990-10-09 United States Of America Method for rapid base sequencing in DNA and RNA
GB8910880D0 (en) * 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
FR2650840B1 (fr) * 1989-08-11 1991-11-29 Bertin & Cie Procede rapide de detection et/ou d'identification d'une seule base sur une sequence d'acide nucleique, et ses applications
WO1991006678A1 (en) * 1989-10-26 1991-05-16 Sri International Dna sequencing
AU2674092A (en) * 1991-09-09 1993-04-05 Baylor College Of Medicine Method and device for rapid dna or rna sequencing determination by a base addition sequencing scheme

Also Published As

Publication number Publication date
EP0640146B1 (de) 1997-10-29
WO1993021340A1 (en) 1993-10-28
ES2110604T3 (es) 1998-02-16
EP0640146A1 (de) 1995-03-01
CA2133956C (en) 2007-02-20
JPH07507681A (ja) 1995-08-31
ATE159766T1 (de) 1997-11-15
US6087095A (en) 2000-07-11
DE69314951D1 (de) 1997-12-04
AU4020893A (en) 1993-11-18
GB9208733D0 (en) 1992-06-10
CA2133956A1 (en) 1993-10-28

Similar Documents

Publication Publication Date Title
DE69314951D1 (de) Dns sequenzierungsverfahren
FI951612A (fi) Menetelmiä synteettisten oligonukleotidien sekvensoimiseksi, jotka sisältävät nukleotidien välisiä ei-fosfodiesterisidoksia
ATE197479T1 (de) Verfahren zur vervielfältigung von nukleinsäuresequenzen
ES2066760T3 (es) Nucleotidos de alquinilamino.
DE69423346D1 (de) Schnelles verfahren fur die dns sequenzierung und diagnostische anwendungen
DE59309183D1 (de) Simultane sequenzierung von nukleinsäuren
ATE239090T1 (de) Methode zum generieren von einzelsträngigen dna molekülen
FR2703052B1 (fr) Nouvelle méthode de séquençage d'acides nucléiques.
DE69315074D1 (de) Verfahren zum kategorisieren von nukleotidsequenz-populationen
ATE353973T1 (de) Die verwendung der 3'-wesentlichen bearbeitungswirksamkeit von dns-polymerase
DE69807962D1 (de) Verfahren zur Reinigung von rohem 1,1,1,3,3-Pentafluorpropan
ES2146309T3 (es) Secuenciacion de acidos nucleicos.
DE3786279T2 (de) Verfahren zur Selektivbestimmung von mikrobiellen Nukleotiden.
FI956052A0 (fi) Salmonellan tunnistus polymeraasiketjureaktorilla
DE69300901D1 (de) Verfahren zur Reinigung von 1,1,1,2-Tetrafluorethan.
DE59608651D1 (de) Verfahren zur sequenzierung durch oligomerhybridisierung
DK0640098T3 (da) S-gen fra hundecoronavirus og anvendelser deraf
DE69637516D1 (de) Verfahren für die Amplifizierung von Nukleinsäure-Sequenzen
PT928191E (pt) Formulacoes farmaceuticas compreendendo derivados de s-alquilisotiouronio
ATE301189T1 (de) Neues verfahren zum nachweis von cryptosporidium

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: ROCHE DIAGNOSTICS GMBH, 68305 MANNHEIM, DE