DE69119083T2 - Direkte klonierung von pcr amplifizierten nukleinsäuren - Google Patents

Direkte klonierung von pcr amplifizierten nukleinsäuren

Info

Publication number
DE69119083T2
DE69119083T2 DE69119083T DE69119083T DE69119083T2 DE 69119083 T2 DE69119083 T2 DE 69119083T2 DE 69119083 T DE69119083 T DE 69119083T DE 69119083 T DE69119083 T DE 69119083T DE 69119083 T2 DE69119083 T2 DE 69119083T2
Authority
DE
Germany
Prior art keywords
nucleic acids
pcr amplified
amplified nucleic
direct cloning
cloning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69119083T
Other languages
English (en)
Other versions
DE69119083D1 (de
Inventor
Corinna Hernstadt
Joseph Fernandez
David Mead
Lloyd Smith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MOLECULAR CHIMERICS CORP MADISON WIS US
Life Technologies Corp
Original Assignee
MOLECULAR CHIMERICS CORP MADISON WIS US
MOLECULAR CHIMERICS CORP
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MOLECULAR CHIMERICS CORP MADISON WIS US, MOLECULAR CHIMERICS CORP, Invitrogen Corp filed Critical MOLECULAR CHIMERICS CORP MADISON WIS US
Application granted granted Critical
Publication of DE69119083D1 publication Critical patent/DE69119083D1/de
Publication of DE69119083T2 publication Critical patent/DE69119083T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
DE69119083T 1990-09-27 1991-09-27 Direkte klonierung von pcr amplifizierten nukleinsäuren Expired - Lifetime DE69119083T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US58981790A 1990-09-27 1990-09-27
PCT/US1991/007147 WO1992006189A1 (en) 1990-09-27 1991-09-27 Direct cloning of pcr amplified nucleic acids

Publications (2)

Publication Number Publication Date
DE69119083D1 DE69119083D1 (de) 1996-05-30
DE69119083T2 true DE69119083T2 (de) 1996-10-17

Family

ID=24359662

Family Applications (3)

Application Number Title Priority Date Filing Date
DE69119083T Expired - Lifetime DE69119083T2 (de) 1990-09-27 1991-09-27 Direkte klonierung von pcr amplifizierten nukleinsäuren
DE69133389T Expired - Lifetime DE69133389T2 (de) 1990-09-27 1991-09-27 Direkte Klonierung von PCR amplifizierten Nukleinsäuren
DE69133559T Expired - Lifetime DE69133559T2 (de) 1990-09-27 1991-09-27 Direkte Klonierung von PCR amplifizierten Nucleinsäuren

Family Applications After (2)

Application Number Title Priority Date Filing Date
DE69133389T Expired - Lifetime DE69133389T2 (de) 1990-09-27 1991-09-27 Direkte Klonierung von PCR amplifizierten Nukleinsäuren
DE69133559T Expired - Lifetime DE69133559T2 (de) 1990-09-27 1991-09-27 Direkte Klonierung von PCR amplifizierten Nucleinsäuren

Country Status (6)

Country Link
US (2) US5487993A (de)
EP (4) EP0550693B1 (de)
AU (1) AU8871891A (de)
DE (3) DE69119083T2 (de)
HK (1) HK1064124A1 (de)
WO (1) WO1992006189A1 (de)

Families Citing this family (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5426039A (en) * 1993-09-08 1995-06-20 Bio-Rad Laboratories, Inc. Direct molecular cloning of primer extended DNA containing an alkane diol
US5994058A (en) * 1995-03-20 1999-11-30 Genome International Corporation Method for contiguous genome sequencing
WO1997048791A1 (en) * 1996-06-18 1997-12-24 Novagen, Inc. Direct cloning of dna fragments
US5856144A (en) * 1997-06-18 1999-01-05 Novagen, Inc. Direct cloning of DNA fragments
US6140086A (en) * 1997-08-15 2000-10-31 Fox; Donna K. Methods and compositions for cloning nucleic acid molecules
CA2307016A1 (en) * 1997-10-24 1999-05-06 Life Technologies, Inc. Recombinational cloning using nucleic acids having recombination sites
WO2000040715A2 (en) * 1999-01-05 2000-07-13 Trustees Of Boston University Improved nucleic acid cloning
US6358712B1 (en) 1999-01-05 2002-03-19 Trustee Of Boston University Ordered gene assembly
US20040005673A1 (en) 2001-06-29 2004-01-08 Kevin Jarrell System for manipulating nucleic acids
JP2000253885A (ja) * 1999-03-11 2000-09-19 Jcr Pharmaceuticals Co Ltd 任意プライマーｐｃｒを用いるｄｎａ断片クローニング方法
WO2000056914A1 (en) * 1999-03-24 2000-09-28 Gene Therapy Systems, Inc. Method for generating transcriptionally active dna fragments
US6709852B1 (en) * 1999-06-22 2004-03-23 Invitrogen Corporation Rapid growing microorganisms for biotechnology applications
US6797509B1 (en) 1999-07-09 2004-09-28 Degussa-Huls Ag Nucleotide sequences which code for the tal gene
US7205129B1 (en) * 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
KR100453279B1 (ko) * 2000-04-27 2004-10-15 주식회사 서린바이오사이언스 양쪽 3'끝이 동일하거나 비상보적인 하나의 염기돌출부위를 갖는 벡터, ＥｃｌＨＫＩ 카세트 및 상기벡터와 ＥｃｌＨＫＩ 카세트의 이용방법
GB0012233D0 (en) 2000-05-19 2000-07-12 Devgen Nv Vector constructs
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
DE10109690A1 (de) 2000-09-02 2002-03-14 Degussa Neue für das metY-Gen kodierende Nukleotidsequenzen
US6544782B1 (en) 2000-11-13 2003-04-08 Synthegen Systems pREM: a positive selection vector system for direct PCR cloning
EP1349917B1 (de) 2000-12-08 2010-03-10 Life Technologies Corporation Verfahren und zusammensetzungen zur synthese von nukleinsäuremolekülen unter verwendung multipler erkennungsstellen
US6566067B2 (en) * 2001-02-14 2003-05-20 Synthegen Systems, Inc. High fidelity PCR cloning
US7060434B2 (en) * 2001-02-14 2006-06-13 Council Of Scientific & Industrial Research Probes for myctophid fish and a method for developing the same
DE10133928A1 (de) * 2001-07-12 2003-01-23 Bayer Cropscience Ag Verfahren zum Herstellen von Deletionsmutanten
KR100441201B1 (ko) * 2001-09-10 2004-07-23 대한민국 리포터 유전자를 포함하는 티벡터용 플라스미드 및 그 제조방법
US6902914B2 (en) * 2001-09-28 2005-06-07 Sigma-Aldrich, Co. Recombinant DNA processes using a dNTP mixture containing modified nucleotides
WO2003030623A2 (en) * 2001-10-12 2003-04-17 Modular Genetics, Inc. System for manipulating nucleic acids
US20030180741A1 (en) 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor
US20040241723A1 (en) * 2002-03-18 2004-12-02 Marquess Foley Leigh Shaw Systems and methods for improving protein and milk production of dairy herds
WO2003091440A1 (fr) * 2002-04-25 2003-11-06 Institute Of Zoology, Chinese Academy Of Sciences Methode de construction d'un vecteur d'expression lineaire sans clonage
US6586237B1 (en) * 2002-05-24 2003-07-01 Kyle David Yesland Compositions and methods for cloning nucleic acids
US6803981B2 (en) * 2002-05-24 2004-10-12 Hannstar Display Corporation Liquid crystal display having biased bending vertical alignment
WO2004009768A2 (en) * 2002-07-18 2004-01-29 Invitrogen Corporation Viral vectors containing recombination sites
KR20050084390A (ko) 2002-12-20 2005-08-26 메타노믹스 게엠베하 운트 코. 카게아아 아미노산의 제조 방법
WO2004065568A2 (en) * 2003-01-23 2004-08-05 Invitrogen Corporation Rapid growing microorganisms for biotechnology applications
EP2390352A1 (de) 2003-03-18 2011-11-30 Quantum Genetics Ireland Limited Systeme und Methoden zur Erhöhung der Milchproduktion bei Tieren
CA2519309A1 (en) 2003-03-25 2004-10-14 Stratagene California Dna polymerase fusions and uses thereof
AU2004262656A1 (en) 2003-08-01 2005-02-17 Basf Plant Science Gmbh Process for the production of fine chemicals in plants
AU2004278752B2 (en) 2003-09-29 2008-04-24 Monsanto Technology Llc Methods for enhancing stress tolerance in plants and methods thereof
ATE469984T1 (de) 2003-12-01 2010-06-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
AU2005244673B2 (en) 2004-02-19 2009-12-10 The Governors Of The University Of Alberta Leptin promoter polymorphisms and uses thereof
CN100465278C (zh) * 2004-06-10 2009-03-04 清华大学 一种构建t载体的方法
US20050281740A1 (en) * 2004-06-16 2005-12-22 Glen Gong Imaging damaged lung tissue
WO2006069610A2 (en) 2004-07-02 2006-07-06 Metanomics Gmbh Process for the production of fine chemicals
EP1974049A2 (de) 2004-12-17 2008-10-01 Metanomics GmbH Verfahren zur steuerung der produktion von feinchemikalien
DE602005020691D1 (de) * 2004-12-23 2010-05-27 Ge Healthcare Bio Sciences Rna-amplifikation auf ligationsbasis
CN101090911B (zh) 2004-12-28 2014-05-07 味之素株式会社 产生l-谷氨酸的微生物和产生l-谷氨酸的方法
EP1841890B1 (de) * 2005-01-03 2013-08-14 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by the Secretary, Department of Health and Human Services Primer für nukleinsäurenachweis
DE102005047596A1 (de) 2005-10-05 2007-04-12 Degussa Ag Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung coryneformer Bakterien
JP5088136B2 (ja) 2005-10-18 2012-12-05 味の素株式会社 コハク酸の製造方法
CA2637600A1 (en) 2006-01-17 2007-07-26 Health Research, Inc. Heteroduplex tracking assay
BRPI0709282B8 (pt) 2006-03-29 2021-05-25 Merial Ltd vacina contra estreptococos
WO2008034648A1 (en) 2006-04-05 2008-03-27 Metanomics Gmbh Process for the production of a fine chemical
US7723103B2 (en) * 2007-01-08 2010-05-25 Lucigen Corporation Vectors, kits and methods for cloning DNA
DE102007005072A1 (de) 2007-02-01 2008-08-07 Evonik Degussa Gmbh Verfahren zur fermentativen Herstellung von Cadaverin
US7881933B2 (en) * 2007-03-23 2011-02-01 Verizon Patent And Licensing Inc. Age determination using speech
EP2149607B1 (de) 2007-04-10 2017-12-20 Ajinomoto Co., Inc. Verfahren zur herstellung von succinylsäure
US20110306099A1 (en) * 2008-11-03 2011-12-15 Matthew Beasley Method of cloning dna
JP2012223091A (ja) 2009-08-25 2012-11-15 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
EP2513304A2 (de) 2009-12-16 2012-10-24 University Of Washington Toxinimmunitätssystem
US8557972B2 (en) * 2009-12-21 2013-10-15 University Of Washington Through Its Center For Commercialization Compositions and methods for transfection of RNA and controlled stabilization of transfected RNA
CA2825357C (en) 2011-02-24 2018-07-10 Inserm (Institut National De La Sante Et De La Recherche Medicale) Igfbp-3 derivatives and uses thereof
RU2507266C2 (ru) * 2011-11-16 2014-02-20 Общество с ограниченной ответственностью "Лаборатория медицинской биотехнологии" (ООО "ЛМБТ") НЕТРАНСКРИБИРУЕМЫЙ ПЛАЗМИДНЫЙ ВЕКТОР pBL-T ДЛЯ КЛОНИРОВАНИЯ СЛОЖНЫХ ГЕНОМНЫХ ПОСЛЕДОВАТЕЛЬНОСТЕЙ И МНОГОМОДУЛЬНОЙ СБОРКИ ГЕННО-ИНЖЕНЕРНЫХ КОНСТРУКЦИЙ (ВАРИАНТЫ), И НАБОР, СОДЕРЖАЩИЙ УКАЗАННЫЙ ВЕКТОР
US10358655B1 (en) 2012-06-27 2019-07-23 University Of Utah Research Foundation Attenuated protein expression vectors and associated methods
DE102012016716A1 (de) 2012-08-22 2014-02-27 Forschungszentrum Jülich GmbH Verfahren zur Herstellung von Vektoren enthaltend ein für in seiner feedback-Inhibierung gemindertes oder ausgeschaltetes Enzym kodierendes Gen und deren Verwendung für die Herstellung von Aminosäuren und Nukleotiden
TWI695067B (zh) 2013-08-05 2020-06-01 美商扭轉生物科技有限公司 重新合成之基因庫
US10669304B2 (en) 2015-02-04 2020-06-02 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA2975855A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
KR20180050411A (ko) 2015-09-18 2018-05-14 트위스트 바이오사이언스 코포레이션 올리고핵산 변이체 라이브러리 및 그의 합성
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
EP3384077A4 (de) 2015-12-01 2019-05-08 Twist Bioscience Corporation Funktionalisierte oberflächen und herstellung davon
WO2017100376A2 (en) 2015-12-07 2017-06-15 Zymergen, Inc. Promoters from corynebacterium glutamicum
EP3478845A4 (de) 2016-06-30 2019-07-31 Zymergen, Inc. Verfahren zur erzeugung einer glucose-permease-bibliothek und verwendungen davon
JP2019519242A (ja) 2016-06-30 2019-07-11 ザイマージェン インコーポレイテッド 細菌ヘモグロビンライブラリーを生成するための方法およびその使用
AU2017315294B2 (en) 2016-08-22 2023-12-21 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
CN110366613A (zh) 2016-12-16 2019-10-22 特韦斯特生物科学公司 免疫突触的变体文库及其合成
US11550939B2 (en) 2017-02-22 2023-01-10 Twist Bioscience Corporation Nucleic acid based data storage using enzymatic bioencryption
CN107058357A (zh) * 2017-03-13 2017-08-18 四川农业大学 一种基于酶切的ta克隆载体及其构建方法和应用
CN110913865A (zh) 2017-03-15 2020-03-24 特韦斯特生物科学公司 免疫突触的变体文库及其合成
JP2020524492A (ja) 2017-06-07 2020-08-20 ザイマージェン インコーポレイテッド Ｃｏｒｙｎｅｂａｃｔｅｒｉｕｍ ｇｌｕｔａｍｉｃｕｍ由来のプロモーターおよび補助遺伝子発現の制御におけるその使用
KR102628876B1 (ko) 2017-06-12 2024-01-23 트위스트 바이오사이언스 코포레이션 심리스 핵산 어셈블리를 위한 방법
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
CA3079613A1 (en) 2017-10-20 2019-04-25 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
CA3088911A1 (en) 2018-01-04 2019-07-11 Twist Bioscience Corporation Dna-based digital information storage
AU2019270243A1 (en) 2018-05-18 2021-01-07 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
KR20210144698A (ko) 2019-02-26 2021-11-30 트위스트 바이오사이언스 코포레이션 항체 최적화를 위한 변이 핵산 라이브러리
KR20210143766A (ko) 2019-02-26 2021-11-29 트위스트 바이오사이언스 코포레이션 Glp1 수용체에 대한 변이체 핵산 라이브러리
US11332738B2 (en) 2019-06-21 2022-05-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
WO2022167396A1 (en) 2021-02-02 2022-08-11 Biocopy Gmbh Method for identifying emerging pathogen mutations enabling host cell entry and immune evasion

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
WO1997048791A1 (en) * 1996-06-18 1997-12-24 Novagen, Inc. Direct cloning of dna fragments
US5856144A (en) * 1997-06-18 1999-01-05 Novagen, Inc. Direct cloning of DNA fragments

Also Published As

Publication number Publication date
DE69133559T2 (de) 2007-11-22
DE69133389T2 (de) 2005-06-02
DE69133389D1 (de) 2004-06-24
DE69133559D1 (de) 2007-03-22
WO1992006189A1 (en) 1992-04-16
EP1396540B1 (de) 2007-02-07
EP1396540A2 (de) 2004-03-10
EP0738779A3 (de) 1997-05-07
EP0550693B1 (de) 1996-04-24
EP1790723A2 (de) 2007-05-30
US5487993A (en) 1996-01-30
EP1790723A3 (de) 2008-10-22
EP0550693A4 (de) 1994-04-27
EP0738779B1 (de) 2004-05-19
US5827657A (en) 1998-10-27
AU8871891A (en) 1992-04-28
HK1064124A1 (en) 2005-01-21
DE69119083D1 (de) 1996-05-30
EP0550693A1 (de) 1993-07-14
EP1396540A3 (de) 2004-06-09
EP0738779A2 (de) 1996-10-23

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Owner name: LIFE TECHNOLOGIES CORP. (N.D.GES.D. STAATES DE, US

Owner name: MOLECULAR CHIMERICS CORP., MADISON, WIS., US

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