DE69114500T2 - Verfahren zum absuchen einer bibliothek. - Google Patents
Verfahren zum absuchen einer bibliothek.Info
- Publication number
- DE69114500T2 DE69114500T2 DE69114500T DE69114500T DE69114500T2 DE 69114500 T2 DE69114500 T2 DE 69114500T2 DE 69114500 T DE69114500 T DE 69114500T DE 69114500 T DE69114500 T DE 69114500T DE 69114500 T2 DE69114500 T2 DE 69114500T2
- Authority
- DE
- Germany
- Prior art keywords
- homologous
- methods
- scaning
- library
- recombination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US55218390A | 1990-07-13 | 1990-07-13 | |
| PCT/US1991/004926 WO1992001069A1 (en) | 1990-07-13 | 1991-07-12 | Library screening method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69114500D1 DE69114500D1 (de) | 1995-12-14 |
| DE69114500T2 true DE69114500T2 (de) | 1996-06-13 |
Family
ID=24204264
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69114500T Expired - Fee Related DE69114500T2 (de) | 1990-07-13 | 1991-07-12 | Verfahren zum absuchen einer bibliothek. |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US5783385A (de) |
| EP (1) | EP0539490B1 (de) |
| JP (1) | JPH05508547A (de) |
| KR (1) | KR930701625A (de) |
| AT (1) | ATE130049T1 (de) |
| AU (2) | AU8299191A (de) |
| CA (1) | CA2086092A1 (de) |
| DE (1) | DE69114500T2 (de) |
| ES (1) | ES2080957T3 (de) |
| IE (1) | IE63212B1 (de) |
| IL (1) | IL98822A0 (de) |
| MX (1) | MX9100215A (de) |
| MY (1) | MY106504A (de) |
| NZ (1) | NZ238957A (de) |
| PT (1) | PT98310B (de) |
| WO (1) | WO1992001069A1 (de) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69114500T2 (de) * | 1990-07-13 | 1996-06-13 | Transkaryotic Therapies Inc | Verfahren zum absuchen einer bibliothek. |
| MX9206979A (es) * | 1991-12-04 | 1993-07-01 | Novo Nordisk As | Metodo de clonacion de proteinas en levaduras |
| FR2689905B1 (fr) * | 1992-04-13 | 1995-07-13 | Orsan | Procede de clonage d'une sequence d'adn codant pour une nadph cytochrome p450 reductase et sequences d'adn codant pour une nadph-cytochrome p450 reductase, notamment de plantes. |
| US5792632A (en) * | 1992-05-05 | 1998-08-11 | Institut Pasteur | Nucleotide sequence encoding the enzyme I-SceI and the uses thereof |
| US5643763A (en) * | 1994-11-04 | 1997-07-01 | Genpharm International, Inc. | Method for making recombinant yeast artificial chromosomes by minimizing diploid doubling during mating |
| US7883872B2 (en) * | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
| US5811381A (en) * | 1996-10-10 | 1998-09-22 | Mark A. Emalfarb | Cellulase compositions and methods of use |
| EP2002846B1 (de) | 1996-12-06 | 2017-01-25 | Amgen Inc. | Kombinationstherapie mit einem IL-1-Inhibitor zur Behandlung von IL-1-vermittelten Krankheiten |
| US6897066B1 (en) | 1997-09-26 | 2005-05-24 | Athersys, Inc. | Compositions and methods for non-targeted activation of endogenous genes |
| US7316923B1 (en) | 1997-09-26 | 2008-01-08 | Athersys, Inc. | Compositions and methods for non-targeted activation of endogenous genes |
| EP1117808B1 (de) * | 1998-10-06 | 2004-12-29 | Mark Aaron Emalfarb | Transformationsystem in filamentösen fungiziden chrysosporium-wirtszellen |
| US6867189B2 (en) * | 2001-07-26 | 2005-03-15 | Genset S.A. | Use of adipsin/complement factor D in the treatment of metabolic related disorders |
| GB0329681D0 (en) * | 2003-12-23 | 2004-01-28 | Delta Biotechnology Ltd | Gene expression technique |
| GB0329722D0 (en) * | 2003-12-23 | 2004-01-28 | Delta Biotechnology Ltd | Modified plasmid and use thereof |
| CN1942222B (zh) * | 2004-03-05 | 2011-08-31 | 麦克罗斯塔克公司 | 用于形成微阀的选择性接合 |
| AU2005299413A1 (en) | 2004-10-22 | 2006-05-04 | Revivicor, Inc. | Ungulates with genetically modified immune systems |
| JP5631533B2 (ja) | 2004-12-23 | 2014-11-26 | ノボザイムス バイオファーマ デーコー アクティーゼルスカブ | 遺伝子発現技術 |
| US9862956B2 (en) | 2006-12-10 | 2018-01-09 | Danisco Us Inc. | Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi |
| EP2102366A4 (de) | 2006-12-10 | 2010-01-27 | Dyadic International Inc | Expression und hochdurchsatz-screening komplexer exprimierter dna-bibliotheken bei fadenpilzen |
| EP2197893B1 (de) * | 2007-09-07 | 2013-07-24 | Dyadic International, Inc. | Neue pilzenzyme |
| EP2348827B1 (de) | 2008-10-27 | 2015-07-01 | Revivicor, Inc. | Immunsupprimierte huftiere |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4038143A (en) * | 1973-10-29 | 1977-07-26 | The Regents Of The University Of Michigan | Test kit for the genetic detection of microorganisms |
| US4745057A (en) * | 1984-05-18 | 1988-05-17 | Eli Lilly And Company | Method, vectors and transformants for high expression of heterologous polypeptides in yeast |
| CA1293460C (en) * | 1985-10-07 | 1991-12-24 | Brian Lee Sauer | Site-specific recombination of dna in yeast |
| US4818700A (en) * | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| US4889806A (en) * | 1987-04-15 | 1989-12-26 | Washington University | Large DNA cloning system based on yeast artificial chromosomes |
| AU614121B2 (en) * | 1988-05-04 | 1991-08-22 | Novartis Ag | Improvements in the production of polypeptides |
| GB8920211D0 (en) * | 1989-09-07 | 1989-10-18 | Ici Plc | Diagnostic method |
| GB8921791D0 (en) * | 1989-09-27 | 1989-11-08 | Imp Cancer Res Tech | Disease gene |
| US5580734A (en) * | 1990-07-13 | 1996-12-03 | Transkaryotic Therapies, Inc. | Method of producing a physical map contigous DNA sequences |
| DE69114500T2 (de) * | 1990-07-13 | 1996-06-13 | Transkaryotic Therapies Inc | Verfahren zum absuchen einer bibliothek. |
| ATE157708T1 (de) * | 1991-08-02 | 1997-09-15 | Transkaryotic Therapies Inc | Methode zum screening einer dna bibliothek |
-
1991
- 1991-07-12 DE DE69114500T patent/DE69114500T2/de not_active Expired - Fee Related
- 1991-07-12 MY MYPI91001262A patent/MY106504A/en unknown
- 1991-07-12 ES ES91913696T patent/ES2080957T3/es not_active Expired - Lifetime
- 1991-07-12 CA CA002086092A patent/CA2086092A1/en not_active Abandoned
- 1991-07-12 WO PCT/US1991/004926 patent/WO1992001069A1/en active IP Right Grant
- 1991-07-12 AU AU82991/91A patent/AU8299191A/en not_active Abandoned
- 1991-07-12 NZ NZ238957A patent/NZ238957A/xx unknown
- 1991-07-12 JP JP91513075A patent/JPH05508547A/ja active Pending
- 1991-07-12 AT AT91913696T patent/ATE130049T1/de not_active IP Right Cessation
- 1991-07-12 EP EP91913696A patent/EP0539490B1/de not_active Expired - Lifetime
- 1991-07-12 PT PT98310A patent/PT98310B/pt not_active IP Right Cessation
- 1991-07-12 KR KR1019930700085A patent/KR930701625A/ko active IP Right Grant
- 1991-07-14 IL IL98822A patent/IL98822A0/xx unknown
- 1991-07-15 MX MX9100215A patent/MX9100215A/es unknown
- 1991-07-15 IE IE247091A patent/IE63212B1/en not_active IP Right Cessation
-
1994
- 1994-09-06 US US08/300,919 patent/US5783385A/en not_active Expired - Fee Related
-
1996
- 1996-02-12 AU AU44493/96A patent/AU689015B2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| CA2086092A1 (en) | 1992-01-14 |
| JPH05508547A (ja) | 1993-12-02 |
| AU4449396A (en) | 1996-06-20 |
| ES2080957T3 (es) | 1996-02-16 |
| IE63212B1 (en) | 1995-04-05 |
| ATE130049T1 (de) | 1995-11-15 |
| US5783385A (en) | 1998-07-21 |
| PT98310A (pt) | 1992-05-29 |
| MY106504A (en) | 1995-06-30 |
| AU8299191A (en) | 1992-02-04 |
| EP0539490B1 (de) | 1995-11-08 |
| EP0539490A1 (de) | 1993-05-05 |
| AU689015B2 (en) | 1998-03-19 |
| IL98822A0 (en) | 1992-07-15 |
| KR930701625A (ko) | 1993-06-12 |
| IE912470A1 (en) | 1992-01-15 |
| NZ238957A (en) | 1994-04-27 |
| DE69114500D1 (de) | 1995-12-14 |
| PT98310B (pt) | 1999-01-29 |
| WO1992001069A1 (en) | 1992-01-23 |
| MX9100215A (es) | 1992-02-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69114500T2 (de) | Verfahren zum absuchen einer bibliothek. | |
| DE69125441T2 (de) | Verfahren zum Gennachweis | |
| DE69411690D1 (de) | Verfahren zum Zementieren von unterirdischen Formationen | |
| DE69225120T2 (de) | Verfahren zum Lysieren von Mykobakterien | |
| DE69807483T2 (de) | Verfahren zum darstellen von glyphen unter verwendung einer bibliothek von gestaltungsdiensten | |
| DE69636723D1 (de) | Verfahren zur Erkundung von zellularen Mobilfunknetzen und Gerät hierfür | |
| DE69535428D1 (de) | Verfahren zum Auffinden von differentiel exprimierte Gene | |
| DE69430054D1 (de) | Verfahren zum einbetten von tierkulturzellen | |
| DE69623640T2 (de) | Verfahren zum trennen von mehrere seismischen energiequellen-signalen | |
| DE68928484D1 (de) | Verfahren zum erkennen von bildstrukturen | |
| DE59605626D1 (de) | Verfahren zur durchführung von kreuzkupplungsreaktionen | |
| DE59301828D1 (de) | Verfahren zum Auffinden von Insertionselementen (IS-Elemente) oder Transposonen | |
| DE69635113D1 (de) | Verfahren zur Erkundung von zellularen Mobilfunknetzen und Gerät hierfür | |
| DE69423574D1 (de) | Verfahren zum Lysieren von Mycobakterien | |
| DE69701364D1 (de) | Verfahren und Rechnerprogramprodukt zum Wiederverwenden von Strukturen zum Durchsuchen von Verzeichnissen | |
| DE69127798D1 (de) | Verfahren und Gerät zum Organisieren und Analysieren von Zeitsteuerungsinformationen | |
| DE69127552D1 (de) | Methode zum screening einer dna bibliothek | |
| DE69803658D1 (de) | Verfahren zum bilden von vinylcarboxylatestern | |
| DE59604726D1 (de) | Verfahren zum Bewegen von Bauwerken | |
| DE69409666D1 (de) | Verfahren unter verwendung von fluorwasserstoffbeständigen keramiken | |
| DE69230666T2 (de) | Verfahren zum stranggiessen von stahl unter verwendung von magnetfeldern | |
| DE69313645T2 (de) | Verfahren zum aufbauen von schweren konstruktionsteilen | |
| DE69132182D1 (de) | System und Verfahren zum Abruf von Zeichenreihen | |
| ATA191091A (de) | Verfahren zum wahlweisen oder kombinierbaren bearbeiten von bahnförmigem material | |
| ATA158390A (de) | Verfahren zum spalten von emulsionen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |