DE602004024034D1 - Nukleinsäureamplifikation auf basis von kügelchenemulsion - Google Patents

Nukleinsäureamplifikation auf basis von kügelchenemulsion

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Publication number
DE602004024034D1
DE602004024034D1 DE602004024034T DE602004024034T DE602004024034D1 DE 602004024034 D1 DE602004024034 D1 DE 602004024034D1 DE 602004024034 T DE602004024034 T DE 602004024034T DE 602004024034 T DE602004024034 T DE 602004024034T DE 602004024034 D1 DE602004024034 D1 DE 602004024034D1
Authority
DE
Germany
Prior art keywords
kinggel
emulsion
nucleic acid
acid amplification
amplification based
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE602004024034T
Other languages
English (en)
Inventor
Jan Berka
Yi-Ju Chen
John H Leamon
Steven Lefkowitz
Kenton Lohman
Vinod Makhijani
Gary J Sarkis
Jonathan Rothberg
Michael Weiner
Maithreyan Srinivasan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
454 Life Science Corp
Original Assignee
454 Life Science Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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Application filed by 454 Life Science Corp filed Critical 454 Life Science Corp
Publication of DE602004024034D1 publication Critical patent/DE602004024034D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
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    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
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    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
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    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6865Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6867Replicase-based amplification, e.g. using Q-beta replicase
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6869Methods for sequencing
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • BPERFORMING OPERATIONS; TRANSPORTING
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    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/582Recycling of unreacted starting or intermediate materials
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/14Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333Saccharide [e.g., DNA, etc.]
DE602004024034T 2003-01-29 2004-01-28 Nukleinsäureamplifikation auf basis von kügelchenemulsion Expired - Lifetime DE602004024034D1 (de)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US44347103P 2003-01-29 2003-01-29
US46507103P 2003-04-23 2003-04-23
US47660203P 2003-06-06 2003-06-06
US47650403P 2003-06-06 2003-06-06
US47631303P 2003-06-06 2003-06-06
US47659203P 2003-06-06 2003-06-06
US49798503P 2003-08-25 2003-08-25
PCT/US2004/002484 WO2004069849A2 (en) 2003-01-29 2004-01-28 Bead emulsion nucleic acid amplification

Publications (1)

Publication Number Publication Date
DE602004024034D1 true DE602004024034D1 (de) 2009-12-24

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ID=32854649

Family Applications (4)

Application Number Title Priority Date Filing Date
DE602004026033T Expired - Lifetime DE602004026033D1 (de) 2003-01-29 2004-01-28 Zweiendige sequenzierung
DE602004022253T Expired - Lifetime DE602004022253D1 (de) 2003-01-29 2004-01-28 Verfahren zur amplifikation und sequenzierung von nukleinsäuren
DE602004036672T Expired - Lifetime DE602004036672C5 (de) 2003-01-29 2004-01-28 Nukleinsäureamplifikation auf Basis von Kügelchenemulsion
DE602004024034T Expired - Lifetime DE602004024034D1 (de) 2003-01-29 2004-01-28 Nukleinsäureamplifikation auf basis von kügelchenemulsion

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Application Number Title Priority Date Filing Date
DE602004026033T Expired - Lifetime DE602004026033D1 (de) 2003-01-29 2004-01-28 Zweiendige sequenzierung
DE602004022253T Expired - Lifetime DE602004022253D1 (de) 2003-01-29 2004-01-28 Verfahren zur amplifikation und sequenzierung von nukleinsäuren
DE602004036672T Expired - Lifetime DE602004036672C5 (de) 2003-01-29 2004-01-28 Nukleinsäureamplifikation auf Basis von Kügelchenemulsion

Country Status (10)

Country Link
US (15) US7244567B2 (de)
EP (8) EP1594980B2 (de)
JP (9) JP2007525151A (de)
CN (2) CN101128601B (de)
AT (4) ATE448302T1 (de)
AU (4) AU2004209426B2 (de)
CA (6) CA2513899C (de)
DE (4) DE602004026033D1 (de)
ES (5) ES2396245T3 (de)
WO (4) WO2005003375A2 (de)

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US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
DK1019496T3 (da) * 1997-07-07 2005-01-10 Medical Res Council In vitro-sorteringsmetode
GB9900298D0 (en) * 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
CA2290731A1 (en) * 1999-11-26 2001-05-26 D. Jed Harrison Apparatus and method for trapping bead based reagents within microfluidic analysis system
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
ATE455186T1 (de) * 2000-06-26 2010-01-15 Nugen Technologies Inc Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren
US6858413B2 (en) 2000-12-13 2005-02-22 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
ATE361996T1 (de) * 2001-03-09 2007-06-15 Nugen Technologies Inc Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen
EP1401850A1 (de) * 2001-06-20 2004-03-31 Nuevolution A/S Nukleosidderivate zur herstellung einer verbindungsbibliothek
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