DE3689411D1 - Methode zur erhöten Expression von Polypeptiden in Hefe des Gattung Pichia. - Google Patents

Methode zur erhöten Expression von Polypeptiden in Hefe des Gattung Pichia.

Info

Publication number
DE3689411D1
DE3689411D1 DE86114700T DE3689411T DE3689411D1 DE 3689411 D1 DE3689411 D1 DE 3689411D1 DE 86114700 T DE86114700 T DE 86114700T DE 3689411 T DE3689411 T DE 3689411T DE 3689411 D1 DE3689411 D1 DE 3689411D1
Authority
DE
Germany
Prior art keywords
pichia
gene
alcohol oxidase
dna sequences
disrupted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE86114700T
Other languages
English (en)
Other versions
DE3689411T2 (de
Inventor
James Michael Cregg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Phillips Petroleum Co
Original Assignee
Phillips Petroleum Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Phillips Petroleum Co filed Critical Phillips Petroleum Co
Application granted granted Critical
Publication of DE3689411D1 publication Critical patent/DE3689411D1/de
Publication of DE3689411T2 publication Critical patent/DE3689411T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
DE3689411T 1985-10-25 1986-10-23 Methode zur erhöten Expression von Polypeptiden in Hefe des Gattung Pichia. Expired - Lifetime DE3689411T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/791,013 US4882279A (en) 1985-10-25 1985-10-25 Site selective genomic modification of yeast of the genus pichia

Publications (2)

Publication Number Publication Date
DE3689411D1 true DE3689411D1 (de) 1994-01-27
DE3689411T2 DE3689411T2 (de) 1994-07-21

Family

ID=25152397

Family Applications (2)

Application Number Title Priority Date Filing Date
DE3689411T Expired - Lifetime DE3689411T2 (de) 1985-10-25 1986-10-23 Methode zur erhöten Expression von Polypeptiden in Hefe des Gattung Pichia.
DE3650749T Expired - Lifetime DE3650749T2 (de) 1985-10-25 1986-10-23 DNA Fragment kodierend für eine AOX

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE3650749T Expired - Lifetime DE3650749T2 (de) 1985-10-25 1986-10-23 DNA Fragment kodierend für eine AOX

Country Status (26)

Country Link
US (1) US4882279A (de)
EP (2) EP0226752B1 (de)
JP (1) JP2614215B2 (de)
KR (1) KR930002738B1 (de)
CN (1) CN86107108A (de)
AT (2) ATE197962T1 (de)
AU (2) AU581107B2 (de)
CA (1) CA1339209C (de)
DD (1) DD255544A5 (de)
DE (2) DE3689411T2 (de)
DK (1) DK510786A (de)
EG (1) EG17985A (de)
ES (2) ES2061433T3 (de)
FI (1) FI98931C (de)
HU (1) HU208552B (de)
IE (1) IE68454B1 (de)
IL (1) IL80286A (de)
IN (1) IN166443B (de)
MX (1) MX168390B (de)
NO (1) NO176923C (de)
NZ (1) NZ217809A (de)
PH (1) PH25990A (de)
PL (1) PL154843B1 (de)
PT (1) PT83618B (de)
YU (1) YU46758B (de)
ZA (1) ZA867650B (de)

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