DE3590766C2 - - Google Patents
Info
- Publication number
- DE3590766C2 DE3590766C2 DE3590766A DE3590766A DE3590766C2 DE 3590766 C2 DE3590766 C2 DE 3590766C2 DE 3590766 A DE3590766 A DE 3590766A DE 3590766 A DE3590766 A DE 3590766A DE 3590766 C2 DE3590766 C2 DE 3590766C2
- Authority
- DE
- Germany
- Prior art keywords
- proteins
- peptides
- genes
- clones
- stochastic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- 230000001151 other effect Effects 0.000 description 1
- 238000004816 paper chromatography Methods 0.000 description 1
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- 229940111202 pepsin Drugs 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- JOHZPMXAZQZXHR-UHFFFAOYSA-N pipemidic acid Chemical compound N1=C2N(CC)C=C(C(O)=O)C(=O)C2=CN=C1N1CCNCC1 JOHZPMXAZQZXHR-UHFFFAOYSA-N 0.000 description 1
- 229940036310 program Drugs 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 238000000163 radioactive labelling Methods 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
- 238000000539 two dimensional gel electrophoresis Methods 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/485—Epidermal growth factor [EGF] (urogastrone)
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1048—SELEX
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/68—Stabilisation of the vector
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
- C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- C—CHEMISTRY; METALLURGY
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- C12N2730/00—Reverse transcribing DNA viruses
- C12N2730/00011—Details
- C12N2730/10011—Hepadnaviridae
- C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
- C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Description
- 1. Es wird der gewünschte Satz von Verbindungen spezifiziert, der die Aufbaublöcke bildet, wobei vorzugsweise eine angemessen große Zahl bestimmter chemischer Spezies verwendet wird, um die Zahl potentieller gleichzeitiger Reak tionen, die zu der gewünschten Zielverbindung führen, zu erhöhen.
- 2. Es wird ein geeignetes Volumen an Reaktions medium verwendet, dazu eine sehr große Zahl neuer stochastischer Proteine zugfügt, die aus transformierten oder transfektierten Zellen, die diese Proteine synthetisieren, iso liert wurden. Es wird ein Versuch ausgeführt, um zu bestimmen, ob die Zielverbindung gebildet wird. Wenn dies der Fall ist, wird sicher gestellt, daß diese Bildung die Gegenwart der Mischung der neuen Proteine erfordert. Wenn dies so ist, sollte die Mischung dann ein Subsortiment von Proteinen enthalten, die eine oder mehrere Reaktionswege, die von dem Satz des Aufbaublocks zu der Zielverbindung führen, katalysieren. Das anfängliche Ensemble von Klonen, die den Satz der neuen stochasti schen Proteine, das Subsortiment, das erfor derlich ist, um die Sequenz von Reaktionen, die zu der Zielverbindung führen, zu kataly sieren, ist zu reinigen und zu teilen.
Claims (23)
in einem gemeinsamen Milieu werden gleichzeitig Gene hergestellt;
die so erhaltenen Gene werden in Wirtszellen eingebracht;
die unabhängigen Klone der transformierten Wirtszellen, die die Gene enthalten, werden gleichzeitig kultiviert, um die Gene zu klonieren und die Bildung von Peptiden, Polypeptiden oder Proteinen, die durch jedes dieser Gene exprimiert werden, zu bewirken;
es wird ein Screening und/oder eine Selektion an solchen Klonen transformierter Wirtszellen durchgeführt, um die Klone zu identifizieren, die Peptide, Polypeptide oder Proteine mit mindestens einer spezifischen Eigenschaft bilden;
die so identifizierten Klone werden isoliert; und in einer Weise vermehrt, um mindestens ein Peptid, Polypeptid oder Protein mit der spezifischen Eigenschaft zu produzieren, dadurch gekennzeichnet, daß die Gene zumindest teilweise aus stochastischen synthetischen Polynukleotiden bestehen.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CH137985 | 1985-03-30 |
Publications (1)
Publication Number | Publication Date |
---|---|
DE3590766C2 true DE3590766C2 (de) | 1991-01-10 |
Family
ID=4209046
Family Applications (7)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE198585902946T Pending DE229046T1 (de) | 1985-03-30 | 1985-06-17 | Verfahren zum erhalten von dns, rns, peptiden, polypeptiden oder proteinen durch dns-rekombinant-verfahren. |
DE19853590766 Pending DE3590766T (de) | 1985-03-30 | 1985-06-17 | |
DE3590766A Expired - Lifetime DE3590766C2 (de) | 1985-03-30 | 1985-06-17 | |
DE3546806A Expired - Lifetime DE3546806C2 (de) | 1985-03-30 | 1985-06-17 | |
DE3546807A Expired - Lifetime DE3546807C2 (de) | 1985-03-30 | 1985-06-17 | |
DE3587814T Expired - Lifetime DE3587814T2 (de) | 1985-03-30 | 1985-06-17 | Verfahren zum erhalten von dns, rns, peptiden, polypeptiden oder proteinen durch dns-rekombinant-verfahren. |
DE3588239T Expired - Lifetime DE3588239T3 (de) | 1985-03-30 | 1985-06-17 | Verfahren zum Erhalten von DNS, RNS, Peptiden, Polypeptiden oder Proteinen durch DMS-Rekombinant-Verfahren |
Family Applications Before (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE198585902946T Pending DE229046T1 (de) | 1985-03-30 | 1985-06-17 | Verfahren zum erhalten von dns, rns, peptiden, polypeptiden oder proteinen durch dns-rekombinant-verfahren. |
DE19853590766 Pending DE3590766T (de) | 1985-03-30 | 1985-06-17 |
Family Applications After (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE3546806A Expired - Lifetime DE3546806C2 (de) | 1985-03-30 | 1985-06-17 | |
DE3546807A Expired - Lifetime DE3546807C2 (de) | 1985-03-30 | 1985-06-17 | |
DE3587814T Expired - Lifetime DE3587814T2 (de) | 1985-03-30 | 1985-06-17 | Verfahren zum erhalten von dns, rns, peptiden, polypeptiden oder proteinen durch dns-rekombinant-verfahren. |
DE3588239T Expired - Lifetime DE3588239T3 (de) | 1985-03-30 | 1985-06-17 | Verfahren zum Erhalten von DNS, RNS, Peptiden, Polypeptiden oder Proteinen durch DMS-Rekombinant-Verfahren |
Country Status (14)
Country | Link |
---|---|
US (7) | US5723323A (de) |
EP (3) | EP0590689B2 (de) |
JP (4) | JP2584613B2 (de) |
CN (1) | CN86102090A (de) |
AU (1) | AU4434585A (de) |
CA (2) | CA1339937C (de) |
CH (1) | CH0229046H1 (de) |
DE (7) | DE229046T1 (de) |
FR (1) | FR2579618B1 (de) |
GB (1) | GB2183661B (de) |
HK (1) | HK20292A (de) |
IN (2) | IN165561B (de) |
SG (1) | SG7992G (de) |
WO (1) | WO1986005803A1 (de) |
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