CN105385587A - Non-contact sensor connector and application thereof - Google Patents
Non-contact sensor connector and application thereof Download PDFInfo
- Publication number
- CN105385587A CN105385587A CN201510981653.7A CN201510981653A CN105385587A CN 105385587 A CN105385587 A CN 105385587A CN 201510981653 A CN201510981653 A CN 201510981653A CN 105385587 A CN105385587 A CN 105385587A
- Authority
- CN
- China
- Prior art keywords
- transparent partition
- basal disc
- conversion film
- sensor probe
- color
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
Landscapes
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention relates to a non-contact sensor connector and application thereof. The non-contact sensor connector comprises a basic disc and an open sensor probe accommodating cavity, wherein a transparent partition part is arranged on the basic disc, the sensor probe accommodating cavity is connected with the basic disc and is located on one side of the transparent partition part, and a conversion film is arranged on the other side of the transparent partition part. The non-contact sensor connector is especially applicable to a closed cell culture device.
Description
Technical field
The present invention relates to non-contacting sensor joint and application thereof.
Background technology
Traditional cell cultures culture dish or culturing bottle are cultivated in carbonic acid gas incubator, require higher to culture condition, need to operate under super clean bench, then be placed in CO2gas incubator and cultivate, and take out to be placed in good time and cause basis of microscopic observation cell growth state.
For the measurement of the pH value in nutrient solution in cell cultivation process, dissolved oxygen amount, generally need contact measurement, easily cause pollution.Even if strict aseptic technique, also be difficult to avoid the not contaminated danger of cell, especially when cultivating the human immunocyte being used for clinical study or clinical treatment, once cell is contaminated, not only there is impact to the cultivation of cell, the life of patient may be threatened simultaneously.
Summary of the invention
For defect of the prior art, the various risk factors that the present invention exists from cell cultivation process are considered, in conjunction with the various conditions of cell cultures, provide a kind of non-contacting sensor joint and application, can without the need to contacting sample, the index of correlation of monitoring cell culture fluid in real time.
The present invention provide firstly a kind of non-contacting sensor joint, comprise basal disc and open sensor probe chamber resettling, described basal disc is provided with transparent partition, described sensor probe chamber resettling is connected with described basal disc and is positioned at the side of described transparent partition, and the opposite side of transparent partition is provided with conversion film.
Preferably, described transparent partition water white transparency, both sides are smooth.
Described sensor probe chamber resettling with sensor probe grafting or can be threaded.
Preferably, described sensor probe chamber resettling is provided with internal thread.
Described conversion film is that pH causes color-changing membrane or dissolved oxygen causes color-changing membrane.
Preferred further, the outer that described transparent partition is provided with the side of conversion film is provided with the bead protruding from basal disc 1.
Described sensor probe chamber resettling can be fixedly connected with basal disc and also can be detachable connection.Detachable mode of connection includes but not limited to grafting, is threaded.
Preferably, described sensor probe chamber resettling and basal disc junction rounding off.
Non-contacting sensor joint of the present invention can be used for enclosed cell culture apparatus.
Described cell culture apparatus includes but not limited to cell culture bags.
The design of non-contacting sensor joint of the present invention, can make with it with the use of sensor without the need to sterilization, not only operate more convenient, and can better Pollution protection.
Accompanying drawing explanation
Fig. 1 is shown as the non-contacting sensor joint schematic diagram of the embodiment of the present invention.
Fig. 2 is shown as the sectional view of the non-contacting sensor joint of the embodiment of the present invention.
Fig. 3 is shown as the stereographic map of the non-contacting sensor joint of the embodiment of the present invention.
Element numbers explanation
1 basal disc
11 transparent partitions
2 sensor probe chamber resettling
21 internal threads
3 beads
Embodiment
By particular specific embodiment, embodiments of the present invention are described below, person skilled in the art scholar the content disclosed by this specification sheets can understand other advantages of the present invention and effect easily.
Refer to Fig. 1 to Fig. 3.Notice, structure, ratio, size etc. that this specification sheets institute accompanying drawings illustrates, content all only in order to coordinate specification sheets to disclose, understand for person skilled in the art scholar and read, and be not used to limit the enforceable qualifications of the present invention, therefore the not technical essential meaning of tool, the adjustment of the modification of any structure, the change of proportionlity or size, do not affecting under effect that the present invention can produce and the object that can reach, still all should drop on disclosed technology contents and obtain in the scope that can contain.Simultaneously, quote in this specification sheets as " on ", D score, "left", "right", " centre " and " one " etc. term, also only for ease of understanding of describing, and be not used to limit the enforceable scope of the present invention, the change of its relativeness or adjustment, under changing technology contents without essence, when being also considered as the enforceable category of the present invention.
Non-contacting sensor joint as Figure 1-3, comprise basal disc 1 and open sensor probe chamber resettling 2, described basal disc 1 is provided with transparent partition 11, described sensor probe chamber resettling 2 is connected with described basal disc 1 and is positioned at the side of described transparent partition 11, and the opposite side of transparent partition 11 is provided with conversion film.
The sensor probe chamber resettling of described opening refers to that described chamber is provided with opening, and described opening can be used for facilitating sensor probe to insert.
Described conversion film and described sensor probe chamber resettling 2 are located at the both sides of described transparent partition 11 respectively.Arrow points shown in Fig. 2 is provided with the side of conversion film.During use, described conversion film contacts with testing liquid, and sensor probe is fixed in sensor probe chamber resettling, relative with conversion film and isolate through transparent the partition with testing liquid.
Described basal disc can be installed container with liquid to be measured and engage.For cell culture bags, can by a side of described basal disc 1 or edge with the welding of described cell culture bags phase or weld, the position of the corresponding transparent partition 11 of cell culture bags is provided with opening, this opening makes the conversion film on transparent partition can contact with nutrient solution in cell culture bags, and the sensor probe inserting sensor probe chamber resettling 2 is then isolated with nutrient solution in cell culture bags due to the existence of transparent partition 11.
Preferably, described transparent partition 11 water white transparency, both sides are smooth.Smooth transparent partition be more conducive to conversion film color through.
Described transparent partition 11 is not limited to the middle part being only located at described basal disc 1, and the basal disc only needing transparent partition periphery to leave one fixed width is enough to combine with cell culture bags.
Described sensor probe chamber resettling 2 with sensor probe grafting or can be threaded.
In a preferred embodiment, described sensor probe chamber resettling 2 is provided with internal thread 21.Coupling, described sensor probe is provided with outside screw.Be threaded and can guarantee the stability that sensor probe detects further.
Described conversion film is that pH causes color-changing membrane or dissolved oxygen causes color-changing membrane.
PH causes color-changing membrane and refers to convert printing opacity color according to the pH value of contact liq.PH causes color-changing membrane and can be obtained by masking after pH indicator and film forming material mixture.PH causes pH described in color-changing membrane and causes color-changing membrane and belong to prior art, as pH electrode the pH sensing diaphragm etc. that uses.
Conversion film is that the sensor connector that pH causes color-changing membrane can match with RGB sensor and white light source.Its mechanism detecting pH value is: pH causes the pH value turn colors of color-changing membrane according to its contact liq, thus affect the color that white light source penetrates this film, white light source coordinates pH to cause color-changing membrane can change pH signal into color signal, the detection of RGB sensor penetrates the color signal of conversion film and transparent partition, relation according to conversion film color and pH value knows that liquid to be measured installs the pH value of liquid to be measured in container, and the relation of conversion film color and pH value can utilize the standardized solution of known pH value to detect in advance and obtain.
Dissolved oxygen causes color-changing membrane can according to the dissolved oxygen value conversion printing opacity color of contact liq.Dissolved oxygen causes color-changing membrane and can be obtained by masking after dissolved oxygen indicator and film forming material mixture.Dissolved oxygen causes dissolved oxygen described in color-changing membrane and causes color-changing membrane and belong to prior art.
Conversion film is that the sensor connector that dissolved oxygen causes color-changing membrane can match with RGB color sensor and white light source.Its mechanism detecting oxygen dissolving value is: dissolved oxygen causes the dissolved oxygen concentration turn colors of color-changing membrane according to its contact liq, thus affect the color that white light source penetrates this film, white light source coordinates dissolved oxygen to cause color-changing membrane can change dissolved oxygen signal into color signal, the detection of RGB color sensor penetrates the color signal of conversion film and transparent partition, relation according to color and dissolved oxygen concentration knows that liquid to be measured installs the dissolved oxygen value of liquid to be measured in container, and the relation between conversion film color and dissolved oxygen can utilize the standardized solution of known dissolved oxygen concentration to detect in advance and obtain.
In order to better protect conversion film, the outer that described transparent partition 11 is provided with the side of conversion film is provided with the bead 3 protruding from basal disc 1.Described bead can alleviate the impact of nutrient solution for conversion film to guarantee the stability that sensor probe detects further.
Described sensor probe chamber resettling 2 can be fixedly connected with basal disc 1 and also can be detachable connection.Detachable mode of connection includes but not limited to grafting, is threaded.
Preferably, described sensor probe chamber resettling 2 and basal disc 1 junction rounding off.
Non-contacting sensor joint of the present invention is particularly useful for cell culture bags.
Through non-contacting sensor joint of the present invention, liquid to be measured installs the pH of liquid to be measured in container and dissolved oxygen situation can be converted into color signal, by accessing RGB sensor probe in sensor probe chamber resettling, color signal can be converted to level signal or square-wave signal, the controller of RGB sensor reads level signal or square-wave signal, the transformational relation of the level signal of answering according to conversion film Color pair and pH value and dissolved oxygen, obtains pH value and the oxygen dissolving value of cell culture fluid.The transformational relation of the level signal that conversion film Color pair is answered and pH value and dissolved oxygen by routine techniques, such as, can obtain by detecting rear curve plotting to the standardized solution of known pH value or oxygen dissolving value in advance.
In sum, the present invention effectively overcomes various shortcoming of the prior art and tool high industrial utilization.
Above-described embodiment is illustrative principle of the present invention and effect thereof only, but not for limiting the present invention.Any person skilled in the art scholar all without prejudice under spirit of the present invention and category, can modify above-described embodiment or changes.Therefore, such as have in art usually know the knowledgeable do not depart from complete under disclosed spirit and technological thought all equivalence modify or change, must be contained by claim of the present invention.
Claims (9)
1. a non-contacting sensor joint, it is characterized in that, comprise basal disc and open sensor probe chamber resettling, described basal disc is provided with transparent partition, described sensor probe chamber resettling is connected with described basal disc and is positioned at the side of described transparent partition, and the opposite side of transparent partition is provided with conversion film.
2. non-contacting sensor joint as claimed in claim 1, it is characterized in that, described transparent partition water white transparency, two sides are smooth.
3. non-contacting sensor joint as claimed in claim 1, it is characterized in that, described transparent partition is located at the middle part of described basal disc.
4. non-contacting sensor joint as claimed in claim 1, it is characterized in that, described sensor probe chamber resettling is provided with internal thread.
5. non-contacting sensor joint as claimed in claim 1, it is characterized in that, described conversion film is that pH causes color-changing membrane or dissolved oxygen causes color-changing membrane.
6. non-contacting sensor joint as claimed in claim 1, it is characterized in that, the outer that described transparent partition is provided with the side of conversion film is provided with the bead protruding from basal disc.
7. non-contacting sensor joint as claimed in claim 1, is characterized in that, described sensor probe chamber resettling and basal disc junction rounding off.
8. the non-contacting sensor joint as described in claim as arbitrary in claim 1-7 is used for the purposes of closed cell culture apparatus.
9. purposes as claimed in claim 8, described cell culture apparatus is cell culture bags.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510981653.7A CN105385587B (en) | 2015-12-23 | 2015-12-23 | A kind of noncontacting proximity sensor joint and its application |
| JP2018533724A JP6889723B2 (en) | 2015-12-23 | 2016-12-15 | Cell culture device |
| US16/064,414 US10934513B2 (en) | 2015-12-23 | 2016-12-15 | Fully automated continuous cell culture system |
| PCT/CN2016/110035 WO2017107837A1 (en) | 2015-12-23 | 2016-12-15 | Fully automated continuous cell culture system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510981653.7A CN105385587B (en) | 2015-12-23 | 2015-12-23 | A kind of noncontacting proximity sensor joint and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105385587A true CN105385587A (en) | 2016-03-09 |
| CN105385587B CN105385587B (en) | 2017-12-05 |
Family
ID=55418373
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510981653.7A Active CN105385587B (en) | 2015-12-23 | 2015-12-23 | A kind of noncontacting proximity sensor joint and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105385587B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017107837A1 (en) * | 2015-12-23 | 2017-06-29 | 上海吉凯基因科技有限公司 | Fully automated continuous cell culture system |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5164796A (en) * | 1988-03-15 | 1992-11-17 | Akzo N.V. | Apparatus and method for detection of microorganisms |
| CN101082578A (en) * | 2006-05-30 | 2007-12-05 | 上海理工大学 | System for detecting light transmission substance thermophysical variation with electrical signal |
| CN201289406Y (en) * | 2008-11-14 | 2009-08-12 | 常州市惠昌传感器有限公司 | Temperature sensor joint |
| US20120097557A1 (en) * | 2009-06-16 | 2012-04-26 | Sartorius Stedim Biotech Gmbh | Container having a sensor adapter |
| CN103162851A (en) * | 2011-12-15 | 2013-06-19 | 西安民生电热技术工程有限责任公司 | Thermosensitive temperature sensor connector with socket |
| US20140000348A1 (en) * | 2012-06-28 | 2014-01-02 | Commissariat A L'energie Atomique Et Aux Energies Alternatives | Location of a leak in a pipe |
| CN203999619U (en) * | 2014-07-17 | 2014-12-10 | 上海美凯纯生物科技有限公司 | A kind of microorganism fast culture proofing unit |
| CN205420410U (en) * | 2015-12-23 | 2016-08-03 | 上海吉凯基因科技有限公司 | Noncontacting proximity sensor connects |
-
2015
- 2015-12-23 CN CN201510981653.7A patent/CN105385587B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5164796A (en) * | 1988-03-15 | 1992-11-17 | Akzo N.V. | Apparatus and method for detection of microorganisms |
| CN101082578A (en) * | 2006-05-30 | 2007-12-05 | 上海理工大学 | System for detecting light transmission substance thermophysical variation with electrical signal |
| CN201289406Y (en) * | 2008-11-14 | 2009-08-12 | 常州市惠昌传感器有限公司 | Temperature sensor joint |
| US20120097557A1 (en) * | 2009-06-16 | 2012-04-26 | Sartorius Stedim Biotech Gmbh | Container having a sensor adapter |
| CN103162851A (en) * | 2011-12-15 | 2013-06-19 | 西安民生电热技术工程有限责任公司 | Thermosensitive temperature sensor connector with socket |
| US20140000348A1 (en) * | 2012-06-28 | 2014-01-02 | Commissariat A L'energie Atomique Et Aux Energies Alternatives | Location of a leak in a pipe |
| CN203999619U (en) * | 2014-07-17 | 2014-12-10 | 上海美凯纯生物科技有限公司 | A kind of microorganism fast culture proofing unit |
| CN205420410U (en) * | 2015-12-23 | 2016-08-03 | 上海吉凯基因科技有限公司 | Noncontacting proximity sensor connects |
Non-Patent Citations (1)
| Title |
|---|
| 蔡伟: "《液压系统非介入式监测技术》", 31 July 2014, 国防工业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017107837A1 (en) * | 2015-12-23 | 2017-06-29 | 上海吉凯基因科技有限公司 | Fully automated continuous cell culture system |
| US10934513B2 (en) | 2015-12-23 | 2021-03-02 | Shanghai GenBase Biotechnology Co., Ltd. | Fully automated continuous cell culture system |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105385587B (en) | 2017-12-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102495051A (en) | Device and method for quickly detecting biological activity and metabolism | |
| EP3134848B1 (en) | Method for detecting micro-colonies growing on a membrane or an agarose medium of a sample and a sterility testing apparatus | |
| CN105385597B (en) | A kind of cell culture bags and its application | |
| BR112015031047B1 (en) | detection system comprising a detection device to detect the presence of at least one microorganism in the contents of a container and its use | |
| CN105385587A (en) | Non-contact sensor connector and application thereof | |
| CN205420410U (en) | Noncontacting proximity sensor connects | |
| CN209178401U (en) | A kind of culture bottle examined fastly suitable for medical device sterile | |
| CN205420436U (en) | Cell culturing bag | |
| CN203999619U (en) | A kind of microorganism fast culture proofing unit | |
| CN201903505U (en) | Portable meat freshness quick-test box | |
| CN203700358U (en) | Disposable rapid microorganism culture and detection dish | |
| CN104673664A (en) | Blood sample culture device | |
| CN206570330U (en) | A kind of microfilament bacterium detection case | |
| CN201006428Y (en) | Sulphate reduction bacterium testing bottle | |
| CN204608020U (en) | The micro-culture apparatus of lymphocyte micronuclei experiment and lymphocyte micronuclei experiment test kit | |
| CN204752714U (en) | Biological sample blood blake bottle | |
| CN101643769A (en) | Test method of microorganism of aircraft fuel oil | |
| CN205420407U (en) | Witnessed inspections total bacteria count's detect reagent box | |
| CN207215830U (en) | A kind of Chloramphenicol detection reagent box | |
| CN2407012Y (en) | Disposable box for testing escherichia coli | |
| CN204807196U (en) | Multi -functional illuminometer | |
| CN204925502U (en) | Glass carrier sheet | |
| CN2689223Y (en) | Blood culturing bottles | |
| CN201006429Y (en) | Bacteria amount testing bottle | |
| CN204314218U (en) | A kind of Novel ATP fluorescence microbial rapid detection instrument |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20160309 Assignee: China (Shanghai) Biological Medicine Co Ltd Assignor: SHANGHAI JIKAI GENE TECHNOLOGY CO., LTD. Contract record no.: 2017310000066 Denomination of invention: Non-contact sensor connector and application thereof Granted publication date: 20171205 License type: Exclusive License Record date: 20171207 |
|
| EE01 | Entry into force of recordation of patent licensing contract | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20171227 Address after: Room 101-7, No. 332, Edison Road, China (Shanghai) free trade test area, Shanghai Patentee after: Shanghai Jibi Biotechnology Co., Ltd. Address before: 201203 room 101-A, No. 328 Edison Road, Zhangjiang hi tech park, Shanghai, Pudong New Area Patentee before: SHANGHAI JIKAI GENE TECHNOLOGY CO., LTD. |
|
| TR01 | Transfer of patent right |