CN104949942A - POCT (point-of-care testing) special protein analysis system - Google Patents
POCT (point-of-care testing) special protein analysis system Download PDFInfo
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- CN104949942A CN104949942A CN201410119925.8A CN201410119925A CN104949942A CN 104949942 A CN104949942 A CN 104949942A CN 201410119925 A CN201410119925 A CN 201410119925A CN 104949942 A CN104949942 A CN 104949942A
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Abstract
The invention discloses a POCT (point-of-care testing) special protein analysis system for measuring the content of C-reactive protein (CRP) in human whole blood; the POCT special protein analysis system comprises a plurality of detection units and a control system for realizing automatic control of the detection process of the C-reactive protein in human whole blood; each detection unit comprises a reaction station, an incident light path system, a transmission light path system and a scattered light path system; a transmission-scattering dual-optical-path system is arranged for detecting the hematokrit of whole blood and the concentration of the C-reactive protein in whole blood respectively, the detection process is convenient and simple, the error caused by complex operation in the prior art can be reduced, and multiple samples can be detected simultaneously; and besides, the POCT special protein analysis system has small volume, high sensitivity and reliable and accurate detection result, can be widely applied to clinically rapid diagnosis of infection and the evaluation of the prognosis after infection for large, middle and small hospitals and outpatient services.
Description
Technical field
The present invention relates to a kind of POCT specific protein analytic system.
Background technology
POCT (point of care testing) is called instant detection, is the system that a kind of volume is little, be convenient for carrying, can on-the-spot detect c reactive protein in people's whole blood.Glycosylated hemoglobin, high-sensitive C-reactive protein etc. are effective test items of a lot of chronic disease, common disease, frequently-occurring disease, have recently occurred some small-sized detecting instruments, make the detection simple and convenient more of c reactive protein in whole blood.
Chinese patent CN102478519B discloses a kind of specific protein analytic system, it comprises at least one composite reaction cup, a combined heated system, the self-propelled machine structure of reagent more than one and a transmission scattering combined optical path system, manually reagent is put into the system after station that detects namely automatically complete heat, stir, haemolysis, the time-sharing automatic interpolation of many reagent, test reaction curve, result of calculation value and printing overall process.When this specific protein analytic system detects, first draw Quantitative Haemolytic agent and put into reaction cup, the blood sample instillation reaction cup of the quantitative collection tester third finger again, reaction cup and the socket of honeycomb fashion reagent bottle are combined and puts into reaction position, then by reagent propulsive mechanism, the detection that suitable ratio color bits carries out specific protein is delivered in reaction position.
Above-mentioned specific protein analytic system can realize the robotization of step except sample of blood, drawn, but this system is applicable to many reagent and measures specific protein, and through self-propelled machine structure, reaction reagent need be delivered to successively than color bits, a sample can only be detected at every turn.So, be necessary to design and be a kind ofly applicable to single reaction reagent, operation is easier and the specific protein analytic system of multiple sample can be detected simultaneously.
Summary of the invention
In order to overcome the problems referred to above, the invention discloses a kind of POCT specific protein analytic system, for measuring the content of c reactive protein and CRP in people's whole blood, this system is applicable to single reagent CRP reactant liquor, has easy and simple to handle, highly sensitive, reproducible, low cost and other advantages.
The concrete technical scheme that the present invention adopts is to provide a kind of POCT specific protein analytic system, for measuring the content of c reactive protein and CRP in people's whole blood, it is characterized in that, described POCT specific protein analytic system comprises some detecting units and the control system for realizing the automatic control to c reactive protein testing process in people's whole blood, and described detecting unit comprises:
A reaction station, for placing the detector tube being preinstalled with CRP reactant liquor;
Input path system, comprises incident light source, and described incident light source is corresponding with described reaction station;
Transmitted light path system, comprises transmitted light receiver and is arranged at the green color filter of described transmitted light receiver front portion, for identifying the adding of people's whole blood, start detection detect erythrocytic hematocrit in whole blood;
Scattering light path system, comprises scattered light receiver, for detecting the extent of reaction of reactant liquor in described detector tube and detecting the concentration of c reactive protein.
Further, described POCT specific protein analytic system is also provided with automaton, described automaton is connected with described transmitted light path system signal, when have whole blood add detector tube time, described transmitted light path system sends signal to automaton, and automaton starts corresponding detecting unit and starts testing.
Further, described automaton is provided with timer, described reaction timer is for setting the reaction time of corresponding detecting unit, when there being whole blood to add detector tube, described reaction timer starts timing, when timing arrives setting-up time, described automaton stops the testing of this corresponding unit, and performs with one or several in issuing orders: display, print, store or transmit the testing result of corresponding unit.
Further, described light forwarding system, transmitted light path system and scattering light path system also arrange incident light through hole, transmitted light through hole and scattered light through hole respectively.
Further, described POCT specific protein analytic system also comprises reactant liquor stirring apparatus, described reactant liquor stirring apparatus comprises the magnetic stir bar be placed in detector tube, stir timer and be arranged at the electromagnet of detector tube outside, and described electromagnet and described stirring timer are electrically connected with described control system.
Further, detector tube inductor is set below described detector tube, for respond to detector tube loading and by Signal transmissions give described control system.
Further, described scattering light path system and described transmitted light path system are 15 ~ 25 degree of angles.
Further, described input path system is single input path, and the wavelength of described single input path is 500 ~ 800 nanometers.
Further, the wavelength-filtered of described green color filter is 500 ~ 600 nanometers.
Further, the wavelength-filtered of described green color filter is 540 ~ 570 nanometers.
Further, described POCT specific protein analytic system also comprises a housing, and several detecting units described and set-up of control system are in described housing.
Further, described housing arranges LCDs, and described LCDs is for showing actions menu, inquiry test result.
The present invention compared with prior art has significant progressive and advantage, pass through technique scheme, adopt single reagent CRP reactant liquor as reaction reagent, arrange transmission scattering double beam system to detect the concentration of packed cell volume in whole blood and c reactive protein respectively, overall process realizes the Automated condtrol added except reaction reagent and whole blood except step by control system, realize robotization to stir, timing and detection, testing process is convenient and simple, the error that in prior art, troublesome operation exists can be reduced, specific protein reactive system of the present invention can detect several different samples simultaneously in addition, greatly improve the service efficiency of machine, reduce the time that user waits for testing result, and specific protein reactive system volume of the present invention is little, structure is simple, highly sensitive, testing result is reliably accurate, can be widely used for large, middle and small hospital, outpatient clinic quick diagnosis infects and infects the evaluation more.
Accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme in the embodiment of the present invention, below the accompanying drawing used required in describing embodiment is briefly described, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the structural representation of the present invention's detecting unit embodiment 1;
Fig. 2 is the structural representation of the present invention's detecting unit embodiment 4;
Fig. 3 is the structural representation of the present invention's detecting unit embodiment 5;
Fig. 4 is the structural representation of reactant liquor stirring apparatus in the present invention;
Fig. 5 is the schematic appearance of a kind of POCT specific protein of the present invention analysis system.
Wherein, 1, reaction station; 2, incident light source; 21, incident light through hole; 3, electromagnet; 31, magnetic stir bar; 4, transmitted light receiver; 41, transmitted light through hole; 42, green color filter; 5, scattered light receiver; 51, scattered light through hole; 6; Base; 7, housing; 71, LCDs; 72, reaction cabin; 8, control device
Embodiment
Below in conjunction with drawings and Examples, the present invention is described in further detail.According to following explanation and claims, advantages and features of the invention will clearly, it should be noted that, accompanying drawing all adopts the form of simplification and non-ratio accurately, only for object that is convenient, the aid illustration embodiment of the present invention lucidly.
Be illustrated in figure 1 the structural representation of the embodiment 1 of a detecting unit in the present invention, this detecting unit is arranged on base 6, comprises reaction station 1, input path system, transmitted light path system, a scattering light path system; Reaction station 1 is arranged on the middle part of base 6, and input path Operation system setting, in the side of base 6, comprises incident light source 2, incident light source 2 is LED light emitting diode, be fixedly installed on base 6, the outer setting outer cover of incident light source 2, the wavelength of incident light is 500 ~ 800 nanometers; Transmitted light path system and scattering light path Operation system setting are at the opposite side of base, transmitted light path system comprises transmitted light receiver 4, green color filter 42, the front of transmission receiver 4 arranges green color filter 42, the wavelength-filtered of green color filter 42 is 500 ~ 600 nanometers, the wavelength-filtered of preferred green color filter 42 is 540 ~ 570 nanometers, green color filter 42 makes ruddiness pass through for filtering green glow, be convenient to transmitted light receiver 4 and receive ruddiness, transmitted light receiver 4 receives ruddiness, and control system just starts to calculate packed cell volume; Scattering light path system comprises scattered light receiver 5, and when scattered light receiver 5 receives scattering light source, namely control system starts the concentration detecting c reactive protein.
In order to optimize the automatic control effect of a kind of POCT specific protein of the present invention analytic system further, in the second embodiment of invention, POCT specific protein analytic system is also provided with automaton, this automaton is connected with transmitted light path system signal, when there being whole blood to add detector tube, transmitted light path system sends signal to automaton, and automaton starts corresponding detecting unit and starts testing.
In order to optimize the automatic control effect of a kind of POCT specific protein of the present invention analytic system further, in the third embodiment of the present invention, reaction timer is provided with at the automaton of the second embodiment of the present invention, described reaction timer is for setting the reaction time of corresponding detecting unit, when have whole blood add detector tube time, described reaction timer starts timing, when timing arrives setting-up time, described automaton stops the testing of this corresponding unit, and perform with one or several in issuing orders: display, print, store, or transmit the testing result of corresponding unit.
In order to avoid a kind of POCT specific protein of the present invention analytic system each detecting unit between light disturbing effect Detection results, as shown in Figure 2, in the 4th kind of embodiment of the present invention, at input path Operation system setting incident light through hole 21, incident light source arrives reaction station by incident light through hole; Transmitted light path Operation system setting transmitted light through hole 41, reaction station 1 connects transmitted light receiver 4 through transmitted light through hole 41; Scattering light path Operation system setting scattered light through hole 51, scattered light through hole 51 is arranged on counterclockwise 15 ~ 25 degree of directions of transmitted light through hole 41, and reaction station 1 connects scattered light receiver 5 through scattered light through hole 51.In order to reduce the light interference between each detecting unit further, also light barrier can be set between each detecting unit.
In order to realize the full and uniform mixing of reactant liquor in detector tube in a kind of POCT of the present invention specific protein analytic system, as shown in Figure 3, in the 5th kind of embodiment of the present invention, reactant liquor stirring apparatus is set, the below of reaction station 1 arranges detector tube inductor (not shown), for respond to detector tube position and by Signal transmissions to control system, namely control system starts reactant liquor stirring apparatus after receiving signal, as shown in Figure 4, this device comprises the magnetic stir bar 31 be placed in detector tube, stir timer and the electromagnet 3 being arranged at detector tube outside, electromagnet 3 and stirring timer are electrically connected with control system 8, namely the signal receiving detector tube inductor when control system 8 starts electromagnet 3 and stirs timer, under the effect of electromagnet 3, the magnetic stir bar 31 of detector tube moves up and down in reactant liquor, reactant liquor is stirred, after stirring 5 ~ 10s, stirring apparatus quits work.
Carry out being positioned over by the detector tube of splendid attire single reagent CRP reactant liquor on reaction position when c reactive protein detects, reactant liquor mixes by the startup of reactant liquor stirring apparatus, and the light that input path system incident light source sends arrives detector tube through incident light through hole; A certain amount of people's whole blood is instilled in detector tube, the color of reactant liquor starts to redden, transmitted light is transmitted optical receiver through green color filter and receives, start reaction timer, start to calculate packed cell volume simultaneously, and scattered light is scattered optical receiver reception through scattered light through hole, control system starts the concentration detecting CRP albumen, detect after 30 ~ 900s and stop, detector tube can being taken out, carry out the detection of next sample.
A detecting unit of the present invention matches with control system and can complete the detection of CRP albumen fast and accurately, conveniently carries 1 ~ 5 detecting unit and control system to be accommodated in a housing just to form a kind of POCT specific protein of the present invention analytic system; If use in laboratory, can arrange more detecting unit in specific protein analytic system, the number of detecting unit can be arranged according to use occasion and needs, does not limit herein.Multiple detecting unit is set and can detects several different samples simultaneously, greatly improve the service efficiency of machine, reduce the time that user waits for testing result, as shown in Figure 5, housing 7 arranges some reaction cabins 72, and the position of reaction cabin 72 is corresponding with the position that detecting unit reacts station; Housing 7 arranges LCDs 71 simultaneously, for showing actions menu and inquiry test result.
The operation steps of a kind of POCT of the present invention specific protein analytic system is as follows:
Step one, start preheating, makes system reach working temperature;
Step 2, blinking red lamp in the screen sash that reaction cabin is corresponding, shows that this reaction station is ready, can detect;
Step 3, is placed in the reaction station of reaction cabin by detector tube, detector tube inductor is by Signal transmissions to control system, and green light flicker in the screen sash that reaction cabin is corresponding, starts reactant liquor stirring apparatus simultaneously and stir timer, the reactant liquor in mixing reaction cup.
Step 4,10 microlitre whole bloods are drawn with kapillary, add in the detector tube mixed, the change (reddening) of transmitted light receiver Quorum Sensing liquid color, start reaction timer, control system converts the content of haemoglobin automatically, and converts packed cell volume to, converses the actual amount adding serum in reaction cup.
Step 5, reactant liquor stirring apparatus stopped afterwards automatically at 5 ~ 10 seconds, and the green light in corresponding screen sash stops flicker, the concentration of scattering light path system start detection CRP.
Step 6, reacted after 30 ~ 900 seconds, and in screen sash, green light reddens lamp, and optical system stops measuring, and in the concentration of liquid crystal display screen display CRP.
Step 7, takes out and detects complete detector tube cup, and now in screen sash, red light starts flicker again, and expression can carry out next sample tests.
The present invention compared with prior art has significant progressive and advantage, adopt single reagent CRP reactant liquor as reaction reagent, arrange transmission scattering double beam system to detect the concentration of packed cell volume in whole blood and c reactive protein respectively, overall process realizes the Automated condtrol added except reaction reagent and whole blood except step by control system, testing process is convenient and simple, the error that in prior art, troublesome operation exists can be reduced, specific protein reactive system of the present invention can detect several different samples simultaneously in addition, greatly improve the service efficiency of machine, reduce the time that user waits for testing result, and specific protein reactive system volume of the present invention is little, structure is simple, highly sensitive, testing result is reliably accurate, can be widely used for large, middle and small hospital, outpatient clinic quick diagnosis infects and infects the evaluation of prognosis.
Above-described specific embodiment; object of the present invention, technical scheme and beneficial effect are further described; be understood that; the foregoing is only specific embodiments of the invention; be not limited to the present invention; within the spirit and principles in the present invention all, any amendment made, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. a POCT specific protein analytic system, for measuring the content of c reactive protein and CRP in people's whole blood, it is characterized in that, described POCT specific protein analytic system comprises at least one detecting unit and the control system for realizing the automatic control to c reactive protein testing process in people's whole blood, and described detecting unit comprises:
A reaction station, for placing the detector tube being preinstalled with CRP reactant liquor;
Input path system, comprises incident light source, and described incident light source is corresponding with described reaction station;
Transmitted light path system, comprises transmitted light receiver and is arranged at the green color filter of described transmitted light receiver front portion, for identifying the adding of people's whole blood, start detection detect erythrocytic hematocrit in whole blood;
Scattering light path system, comprises scattered light receiver, for detecting the extent of reaction of reactant liquor in described detector tube and testing the concentration of c reactive protein.
2. POCT specific protein analytic system according to claim 1, it is characterized in that: described POCT specific protein analytic system is also provided with automaton, described automaton is connected with described transmitted light path system signal, when there being whole blood to add detector tube, described transmitted light path system sends signal to automaton, and automaton starts corresponding detecting unit and starts testing.
3. POCT specific protein analytic system according to claim 2, it is characterized in that: described automaton is provided with reaction timer, described reaction timer is for setting the reaction time of corresponding detecting unit, when there being whole blood to add detector tube, described reaction timer starts timing, when timing arrives setting-up time, described automaton stops the testing of this corresponding unit, and performs with one or several in issuing orders: display, print, store or transmit the testing result of corresponding unit.
4. POCT specific protein analytic system according to claim 3, is characterized in that: described light forwarding system, transmitted light path system and scattering light path system also arrange incident light through hole, transmitted light through hole and scattered light through hole respectively.
5. POCT specific protein analytic system according to claim 4, it is characterized in that: described detecting unit also comprises reactant liquor stirring apparatus, described reactant liquor stirring apparatus comprises the magnetic stir bar be placed in detector tube, stir timer and be arranged at the electromagnet of detector tube outside, and described electromagnet and described stirring timer are electrically connected with described control system.
6. POCT specific protein analytic system according to claim 5, is characterized in that: arrange detector tube inductor below described detector tube, for respond to detector tube loading and by Signal transmissions give described control system.
7. want the POCT specific protein analytic system described in 6 according to right, it is characterized in that: described scattering light path system and described transmitted light path system are 15 ~ 25 degree of angles.
8. POCT specific protein analytic system according to claim 7, is characterized in that: described input path system is single input path, the wavelength of described single input path is 500 ~ 800 nanometers.
9. POCT specific protein analytic system according to claim 8, is characterized in that: the wavelength-filtered of described green color filter is 500 ~ 600 nanometers.
10. POCT specific protein analytic system according to claim 1, is characterized in that: described POCT specific protein analytic system also comprises a housing, and described detecting unit and set-up of control system are in described housing.
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| CN201410119925.8A CN104949942A (en) | 2014-03-27 | 2014-03-27 | POCT (point-of-care testing) special protein analysis system |
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| CN201410119925.8A CN104949942A (en) | 2014-03-27 | 2014-03-27 | POCT (point-of-care testing) special protein analysis system |
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Cited By (6)
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| CN105548020A (en) * | 2015-12-09 | 2016-05-04 | 苏州德沃生物技术有限公司 | Full-automatic multichannel biochemical detector and detection method thereof |
| CN105784642A (en) * | 2016-05-23 | 2016-07-20 | 深圳市国赛生物技术有限公司 | Detection device and optical system thereof |
| CN108088816A (en) * | 2018-01-23 | 2018-05-29 | 深圳市国赛生物技术有限公司 | Small-sized specific protein analyzer |
| CN108732135A (en) * | 2017-11-20 | 2018-11-02 | 重庆中元汇吉生物技术有限公司 | A kind of blood cell and analysis of protein device |
| JPWO2018034208A1 (en) * | 2016-08-18 | 2019-06-13 | コニカミノルタ株式会社 | Measuring method |
| CN113777076A (en) * | 2021-08-30 | 2021-12-10 | 四川南格尔生物科技有限公司 | Sensor and method for online real-time concentration monitoring |
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| CN113777076A (en) * | 2021-08-30 | 2021-12-10 | 四川南格尔生物科技有限公司 | Sensor and method for online real-time concentration monitoring |
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Application publication date: 20150930 |