CN104880440A - Standard color card, production method of standard color card and biological analysis detection set - Google Patents
Standard color card, production method of standard color card and biological analysis detection set Download PDFInfo
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- CN104880440A CN104880440A CN201510236414.9A CN201510236414A CN104880440A CN 104880440 A CN104880440 A CN 104880440A CN 201510236414 A CN201510236414 A CN 201510236414A CN 104880440 A CN104880440 A CN 104880440A
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Abstract
The invention provides a standard color card which is used for calibrating a fluorescent material based biological analysis detector. The standard color card comprises a shell with a testing window and a base material encapsulated in the shell, wherein a plurality of color development layers which are arranged linearly and of which the light intensities are changed in a gradient manner are formed on the surface of the base material and correspond to the testing window, each color development layer contains a semiconductor nanocrystalline material. The invention also relates to a production method of the standard color card and a biological analysis detection set provided with the standard color card.
Description
Technical field
The present invention relates to a kind of standard color card, standard color card method for making and bioanalysis and detect external member.
Background technology
At present, bioanalysis detector based on fluorescent material carries out the Cleaning Principle of project based on light reflection principle, the light source (as blue light) of the certain wavelength of usual employing and intensity irradiates, the label on base material is made to send fluorescence, the fluorescence signal being tried bar reflects by chromatography by optoelectronic sensor again changes electric signal into, draw matched curve, the area or the intensity that calculate test value curve crest determine corresponding fluorescence signal intensity, thus set up the relation between electric signal and tested sample concentration.
So based on the bioanalysis detector of fluorescent material in the process determining fluorescence intensity, the optoelectronic sensor due to this bioanalysis detector is subject to the impact of signal source intensity, environment for use factor etc. and detector in long-time use procedure due to equipment consume, aging and cause the degree of deviation of testing result to increase.Therefore, before carrying out project detection, if disappearance is calibrated the bioanalysis detector based on fluorescent material, by impact based on the consistance of the bioanalysis detector testing result of fluorescent material and accuracy.
Summary of the invention
In view of above content, be necessary the standard color card of the bioanalysis detector testing result degree of deviation providing a kind of reduction based on fluorescent material, standard color card method for making and be equipped with this standard color card bioanalysis detect external member.
A kind of standard color card, for calibrating the bioanalysis detector based on fluorescent material.This standard color card comprises the shell offering test window and the base material be packaged in this shell.This substrate surface is to the position of test window being formed with the chromonic layer that some linearly aligned luminous intensities change in gradient.Each chromonic layer contains semiconductor nano material.
This base material is selected from least one in tygon, polypropylene, Polyvinylchloride, polyester, expanded polystyrene (EPS), acrylonitrile-butadiene-styrene copolymer, nylon, polyimide, teflon, polyphenylene sulfide, polysulfones, polymethylmethacrylate polycarbonate.
Each chromonic layer parallel interval arranges.
The pattern that each chromonic layer is formed on the substrate is point-like or is band shape, and the pattern diameter of each chromonic layer or width are 20 ~ 2000 microns.
This semiconductor nano material is selected from CdSe, CdTe, CdS, ZnS, ZnSe, Zn
xcd
1-xse (0≤x≤1) or Cu
xin
1-xs
2(0<x<1) at least one in the nanocrystalline material of nucleocapsid structure or its combination.
This bioanalysis detector comprises the test card that is provided with detection zone, and this chromonic layer is corresponding with the standard luminescent intensity that this bioanalysis detector detects the detection zone of this test card.
A method for making for standard color card, it comprises the steps:
The standard solution of configuration variable concentrations, this standard solution is that semiconductor nano material is dissolved in organic phase or the configuration that is soluble in the aqueous phase forms;
By the standard solution linear print of this variable concentrations or printing on substrate surface, to form the chromonic layer that some linearly aligned luminous intensities change in gradient at this substrate surface;
This base material to be packaged in the shell with test window thus obtained standard color card.
This semiconductor nano material is selected from CdSe, CdTe, CdS, ZnS, ZnSe, Zn
xcd
1-xse (0≤x≤1) or Cu
xin
1-xs
2(0<x<1) at least one in the nanocrystalline material of nucleocapsid structure or its combination.
This printing type adopts plotting apparatus, printing of inkjet printer, and this mode of printing adopts serigraphy, prints or printing to be positioned at this substrate surface by the standard solution of variable concentrations gradient.
Each chromonic layer is point-like or band shape at the pattern that this substrate surface is formed, and the pattern diameter of each chromonic layer or width are 20 ~ 2000 microns.
A kind of bioanalysis detects external member, and it comprises bioanalysis detector and the standard color card for calibrating this bioanalysis detector.This standard color card comprises the shell offering test window and the base material be packaged in shell.This substrate surface is to the position of test window being formed with the chromonic layer that some linearly aligned luminous intensities change in gradient.Each chromonic layer contains semiconductor nano material.
This bioanalysis detector be adopt that fluorescence immune chromatography quantitatively detects, the detection of fluorescence immunoassay determining adsorption, time-resolved fluorescence, in situ hybridization, biochip and microflow control technique quantitative checkout equipment at least one.
Compare prior art, above-mentioned standard color card, by printing on the base material of this standard color card or printing the chromonic layer changed in gradient with the luminous intensity forming some variable concentrations, wherein each chromonic layer contains semiconductor nano material, this chromonic layer is corresponding with the standard luminescent intensity of the detection zone that this bioanalysis detector detects, thus reduces the degree of deviation based on the bioanalysis detector testing result of fluorescent nano material.The standard color card favorable repeatability that the present invention makes, accuracy are high, with low cost.The bioanalysis being equipped with the calibration of this standard color card detects external member, and its testing result accuracy is high.
Accompanying drawing explanation
Fig. 1 is the vertical view of the standard color card of a preferred embodiment of the present invention.
Fig. 2 is the vertical view of the standard color card of another preferred embodiment of the present invention.
Fig. 3 is the structural drawing that bioanalysis of the present invention detects external member.
Fig. 4 is the calibration standard curve schematic diagram of the standard color card that embodiment 3 makes.
Main element symbol description
| Standard color card | 100，200 |
| Shell | 10，421 |
| Test window | 11，420 |
| Base material | 20，30 |
| Chromonic layer | 21，31 |
| Bioanalysis detects external member | 400 |
| Bioanalysis detector | 41 |
| Draw-in groove | 411 |
| Test card | 42 |
| Fluorescence immune chromatography examination bar | 422 |
| Detection zone | 423 |
| Quality control band | 424 |
Following embodiment will further illustrate the present invention in conjunction with above-mentioned accompanying drawing.
Embodiment
Refer to Fig. 1 and Fig. 3, the structural drawing of the first better embodiment of standard color card 100 of the present invention, this standard color card 100 is for calibrating fluorescence immune chromatography instrument.This standard color card 100 comprises shell 10 and is packaged in the base material 20 in shell 10.This shell 10 offers a test window 11.This shell 10 is plastic casing.
This base material 20 is selected from tygon (PE), polypropylene (PP), Polyvinylchloride (PVC), polyester (PV), expanded polystyrene (EPS) (EPS), acrylonitrile-butadiene-styrene copolymer (ABS), nylon (PA), polyimide (PI polyimide), teflon (PET), polyphenylene sulfide (PS), polysulfones (PES), at least one in polymethylmethacrylate (PMMA) polycarbonate (PC Polycarbonate).
Understandable, this base material 20 can make transparent base, also can make opaque base material.
In the present embodiment, this base material 20 is PVC, PET or PI polyimide.
The surface of this base material 20 is to the position of test window printing or print the chromonic layer 21 that some linearly aligned luminous intensities change in gradient, and each chromonic layer 21 is containing semiconductor nano material.
Preferably, each chromonic layer 21 parallel interval arrangement.
This chromonic layer 21 forms the band pattern of sequential on the same surface of this base material 20, and the bandwidth width control system of each chromonic layer 21 is on any yardstick of 20 microns to 2000 microns.This semiconductor nano material is dissolved in organic phase or is soluble in the aqueous phase, and this semiconductor nano material is selected from cadmium selenide (CdSe), cadmium telluride (CdTe), cadmium sulfide (CdS), zinc selenide (ZnS), zinc selenide (ZnSe), Zn
xcd
1-xse (0≤x≤1) or Cu
xin
1-xs
2(0<x<1) at least one in the nanocrystalline material of nucleocapsid structure or its combination.This semiconductor nano material fluorescence emission wavelengths scope is 450 ~ 1000 nanometers (nm).
This semiconductor nano material is a kind of quantum dot, and preferably, it selects fluorescence quantum yield be greater than the method preparation of 50% and obtain.
Refer to Fig. 2, it is the structural drawing of another preferred embodiment of standard color card 200 of the present invention.This standard color card 200, this standard color card 200 is for calibrating fluorescence immunoassay determining adsorption instrument, and this standard color card 200 comprises base material 30 and encapsulates the shell (not shown) of this base material 30.This shell is packaging adhesive film.This base material 30 is selected from least one in PE, PP, PVC, PV, EPS, ABS, PA, PI polyimide, PET, PS, PES, PMMA, PC Polycarbonate.
Understandable, this base material 30 can make transparent base, also can make opaque base material.
In the present embodiment, this base material 30 is PET.This PET is nested in 96 orifice surfaces.
In each plate hole of 96 orifice plates being nested with PET, print or print the chromonic layer 31 that some linearly aligned luminous intensities change in gradient, each chromonic layer 31 is containing semiconductor nano material.
Preferably, each chromonic layer parallel interval arrangement.
This chromonic layer 31 forms the dot pattern of sequential on the same surface of this base material 30, and this dot pattern is considered as standard point.This dot pattern is such as circular, square etc., and the width (or diameter) of this dot pattern is usually also between 20 microns to 2000 microns.This semiconductor nano material is dissolved in organic phase or is soluble in the aqueous phase, and this semiconductor nano material is selected from CdSe, CdTe, CdS, ZnS, ZnSe, Zn
xcd
1-xse (0≤x≤1) or Cu
xin
1-xs
2(0<x<1) at least one in the nanocrystalline material of nucleocapsid structure or its combination.This semiconductor nano material fluorescence emission wavelengths scope is 450 ~ 1000 nanometers (nm).
This semiconductor nano material is a kind of quantum dot, and preferably, it selects fluorescence quantum yield be greater than the method preparation of 50% and obtain.
Understandable, the difference that standard color card of the present invention is chosen according to base material, can select at least one in reflection or transmission mode to carry out the calibration of this fluorescence immune chromatography instrument.When this base material is opaque base material, reflection mode is selected to calibrate.When this base material is transparent base, transmission mode is selected to calibrate.
Understandable, standard color card of the present invention is not limited to fluorescence immune chromatography instrument in fair copy embodiment and fluorescence immunoassay determining adsorption instrument, also can be used for calibration in situ hybridization, biochip and the various bioanalysis detector based on fluorescent material such as micro-fluidic, in addition this standard color card also can detect by binding time resolved fluorometric, thus ensure that consistance and the accuracy of the testing result of each bioanalysis detector.
The method for making of the standard color card 100 that the present invention is above-mentioned, it comprises the steps:
First, base material 20 is chosen.
Understandable, this base material 20 is selected from least one in PE, PP, PVC, PV, EPS, ABS, PA, PI polyimide, PET, PS, PES, PMMA, PC Polycarbonate.
Secondly, configuration standard solution.
Containing the semiconductor nano material being dissolved in organic phase or be soluble in the aqueous phase in this standard solution.Understandable, this standard solution at least configures the solution of 2 concentration gradients, and can adjust desired concn gradient according to actual conditions.Preferably, standard solution configures the solution of 5 concentration gradients.
This semiconductor nano material is selected from CdSe, CdTe, CdS, ZnS, ZnSe, Zn
xcd
1-xse (0≤x≤1) or Cu
xin
1-xs
2(0<x<1) at least one in the nanocrystalline material of nucleocapsid structure or its combination.
Then, adopt printing device or printing equipment by the standard solution linear print of this variable concentrations or be printed on the surface of this base material 20, to form the chromonic layer 21 that some linearly aligned luminous intensities change in gradient on this base material 20 surface.
Understandable, the concentration of standard solution is higher, and the luminous intensity of its chromonic layer 21 printing or print is larger.Each chromonic layer 21 is corresponding with the standard luminescent intensity detected by this fluorescence immune chromatography instrument.Each chromonic layer 21 forms point-like or band pattern on the surface at this base material 20.The bandwidth width of each chromonic layer 21 or the usual scope control of diameter are on any yardstick of 20 microns to 2000 microns.
Understandable, this printing device is such as plotting apparatus, ink-jet printer.This printing equipment is such as serigraphy.Print or printing the standard solution of variable concentrations gradient is positioned on base material 20 surface.
Subsequently, this base material 20 is carried out encapsulating thus obtains standard color card 100.
Understandable, suitable cutting can be carried out to this base material 20 before packaging.Such as adopt PVC to be base material 20, with cut-off knife, this base material 20 need be cut into the size of fluorescence immune chromatography examination bar 422.
Understandable, encapsulation process can adopt shell 10 to encapsulate, and this shell 10 can be at least one in plastic casing or packaging adhesive film.
Refer to Fig. 3, bioanalysis of the present invention detects the structural drawing of external member 400, and this bioanalysis detects external member 400 and comprises bioanalysis detector 41 and the standard color card 100 for calibrating this bioanalysis detector 41.This bioanalysis detector 41 comprises test card 42.
In the present embodiment, this bioanalysis detector 41 is fluorescence immunoassay equipment, such as, be a fluorescence immune chromatography instrument.
Understandable, this standard color card 100 is measure-alike with this test card 42.
This test card 42 comprises shell 421 and is packaged in the fluorescence immune chromatography examination bar 422 in shell 421.This shell 421 offers a test window 420.This fluorescence immune chromatography examination bar 422 surface is to the position of test window being provided with detection zone 423 and a quality control band 424.
This standard color card 100 comprises shell 10 and is packaged in the base material 20 in shell 10.This shell 10 offers a test window 11.This shell 10 is plastic casing.This base material 20 surface prints in the test window position of correspondence or prints the chromonic layer 21 that some linearly aligned luminous intensities change in gradient, and each chromonic layer 21 is containing semiconductor nano material.
Understandable, this base material 20 is identical with the size that this fluorescence immune chromatography tries bar 422.
This bioanalysis detector 41 is provided with a draw-in groove 411.This bioanalysis detector 41 is before test item detects, first this standard color card 100 is inserted in the draw-in groove 411 of this bioanalysis detector 41, and the fluorescence intensity level of corresponding chromonic layer 21 is read from this bioanalysis detector 41, and the fluorescence intensity level that this chromonic layer 21 records is designated as T value, determine the fluorescence signal intensity that each chromonic layer 21 on this standard color card 100 sends.Subsequently according to the relation of concentration of the T value read and known standard solution, structure canonical plotting.After typical curve builds, again this test card 42 is inserted in the draw-in groove 411 of this bioanalysis detector 41, read the fluorescence intensity level of corresponding detection zone 423 from this bioanalysis detector 41, and according to the canonical plotting set up correct the fluorescence intensity level that this bioanalysis detector 41 records in this detection zone 423.
In the present embodiment, this bioanalysis detector 41 is fluorescence immunoassay equipment, such as, be a fluorescence immune chromatography quantitative detecting analysis detector.
Understandable, this bioanalysis detector can also be at least one in the quantitative checkout equipment of such as fluorescence immunoassay determining adsorption, time-resolved fluorescence detection, in situ hybridization, biochip and microflow control technique.
Below by specific embodiment, the present invention is described further.
Embodiment 1
Method for making for the standard color card of fluorescence immune chromatography instrument:
PVC is selected to be base material.Select the CdSe/ZnS semiconductor nano material be soluble in the aqueous phase, compound concentration is the standard solution 5mL of 0.5mg/mL, 5mg/mL respectively.Adopt plotting apparatus by above-mentioned 2 kinds of standard solution respectively linearly mode mode print on base material, to form the chromonic layer that 2 luminous intensities change in gradient on base material one surface, each chromonic layer forms the band pattern of parallel interval arrangement on same surface, and the bandwidth width of each chromonic layer is 1000 microns.With cut-off knife, this base material is cut into the size of fluorescence immune chromatography examination bar, and loaded plastic casing encapsulation.
Embodiment 2
Method for making for the standard color card of fluorescence immunoassay determining adsorption instrument:
96 orifice plates being nested with PET are selected to be base material.Select the CdSe/ZnS semiconductor nano material be soluble in the aqueous phase, compound concentration is the standard solution 5mL of 0.1mg/mL, 0.2mg/mL, 0.5mg/mL, 5mg/mL, 10mg/mL respectively.Adopt plotting apparatus by above-mentioned 5 kinds of standard solution respectively linearly dot pattern print on 96 orifice plates, to form the chromonic layer that 5 luminous intensities change in gradient in the plate hole of 96 orifice plates, each chromonic layer forms the circular pattern of parallel interval arrangement in the plate hole of 96 orifice plates, and the diameter of each chromonic layer is 1000 microns.Packaging adhesive film is adopted to encapsulate base material.
Embodiment 3
Method for making for the standard color card of fluorescence immune chromatography instrument:
PI polyimide is selected to be base material.Select the CdSe/ZnS semiconductor nano material being dissolved in organic phase, compound concentration is the standard solution 5mL of 0.01mg/mL, 0.02mg/mL, 0.05mg/mL, 0.5mg/mL, 1mg/mL respectively.Adopt plotting apparatus by above-mentioned 5 kinds of standard solution respectively linearly mode mode print on base material, to form the chromonic layer that 5 luminous intensities change in gradient on a surface, each chromonic layer forms the band pattern of parallel interval arrangement on the same surface of base material, and the bandwidth width of each chromonic layer is 1000 microns.With cut-off knife, this base material is cut into the size of fluorescence immune chromatography examination bar, and loaded plastic casing encapsulation.
Refer to Fig. 4, typical curve linear relationship master is: y=2776.x+33.716, R
2=0.99937.Wherein, R
2for related coefficient square, R
2more close to 1, its linear relationship is better, and experiment shows that the linear relationship of typical curve provided by the invention is better.
As shown in Table 1, be the fluorescence intensity level (being designated as T value) of 5 chromonic layer that standard color card that embodiment 3 makes records.First the standard color card of preparation in embodiment 3 is inserted fluorescence immune chromatography instrument, and the fluorescence signal intensity that each chromonic layer settled the standard on colour atla sends.The difference of the luminescence power of the chromonic layer then printed according to standard color card or print, reads corresponding T value from fluorescence immune chromatography instrument.Subsequently using the T value that reads as standard value, and the relation of standard solution according to these standard values and concentration known, structure canonical plotting as shown in Figure 4.According to the testing result that set up canonical plotting and the quantitative checkout equipment of recoverable record.
The T value of 5 chromonic layer that the standard color card that table one embodiment 3 makes records
Understandable, in other embodiments, a surface of this base material can also have nitrocellulose filter.The surface of this nitrocellulose filter is to the position of test window printing or print the chromonic layer that some linearly aligned luminous intensities change in gradient, and each chromonic layer contains semiconductor nano material.Subsequently this nitrocellulose filter is bonded at this substrate surface, and cuts into the size of fluorescence immunoassay examination bar.
Above-described embodiment is the present invention's preferably embodiment, but embodiments of the present invention are not restricted to the described embodiments, and above embodiment is only for explaining claims.Right protection scope of the present invention is not limited to instructions.Anyly be familiar with those skilled in the art in the technical scope that the present invention discloses, the change that can expect easily or replacement, be included within protection scope of the present invention.
Claims (12)
1. a standard color card, for calibrating the bioanalysis detector based on fluorescent material, this standard color card comprises the shell offering test window and the base material be packaged in this shell, this substrate surface is to the position of test window being formed with the chromonic layer that some linearly aligned luminous intensities change in gradient, and each chromonic layer contains semiconductor nano material.
2. standard color card as claimed in claim 1, is characterized in that: this base material is selected from least one in tygon, polypropylene, Polyvinylchloride, polyester, expanded polystyrene (EPS), acrylonitrile-butadiene-styrene copolymer, nylon, polyimide, teflon, polyphenylene sulfide, polysulfones, polymethylmethacrylate polycarbonate.
3. standard color card as claimed in claim 1, is characterized in that: each chromonic layer parallel interval arranges.
4. standard color card as claimed in claim 1, it is characterized in that: the pattern that each chromonic layer is formed on the substrate is point-like or is band shape, and the pattern diameter of each chromonic layer or width is 20 ~ 2000 microns.
5. standard color card as claimed in claim 1, is characterized in that: this semiconductor nano material is selected from CdSe, CdTe, CdS, ZnS, ZnSe, Zn
xcd
1-xse (0≤x≤1) or Cu
xin
1-xs
2(0<x<1) at least one in the nanocrystalline material of nucleocapsid structure or its combination.
6. standard color card as claimed in claim 1, it is characterized in that: this bioanalysis detector comprises the test card that is provided with detection zone, this chromonic layer is corresponding with the standard luminescent intensity that this bioanalysis detector detects the detection zone of this test card.
7. a method for making for standard color card, it comprises the steps:
The standard solution of configuration variable concentrations, this standard solution is that semiconductor nano material is dissolved in organic phase or the configuration that is soluble in the aqueous phase forms;
By the standard solution linear print of this variable concentrations or printing on substrate surface, to form the chromonic layer that some linearly aligned luminous intensities change in gradient at this substrate surface;
This base material to be packaged in the shell with test window thus obtained standard color card.
8. the method for making of standard color card as claimed in claim 7, is characterized in that: this semiconductor nano material is selected from CdSe, CdTe, CdS, ZnS, ZnSe, Zn
xcd
1-xse (0≤x≤1) or Cu
xin
1-xs
2(0<x<1) at least one in the nanocrystalline material of nucleocapsid structure or its combination.
9. the method for making of standard color card as claimed in claim 7, it is characterized in that: this printing type adopts plotting apparatus, printing of inkjet printer, this mode of printing adopts serigraphy, prints or printing to be positioned at this substrate surface by the standard solution of variable concentrations gradient.
10. the method for making of standard color card as claimed in claim 7, it is characterized in that: each chromonic layer is point-like at the pattern that this substrate surface is formed or is band shape, and the pattern diameter of each chromonic layer or width is 20 ~ 2000 microns.
11. 1 kinds of bioanalysiss detect external member, it comprises bioanalysis detector and the standard color card for calibrating this bioanalysis detector, this standard color card comprises the shell offering test window and the base material be packaged in shell, this substrate surface is to the position of test window being formed with the chromonic layer that some linearly aligned luminous intensities change in gradient, and each chromonic layer contains semiconductor nano material.
12. bioanalysiss as claimed in claim 11 detect external members, it is characterized in that: this bioanalysis detector be adopt that fluorescence immune chromatography quantitatively detects, the detection of fluorescence immunoassay determining adsorption, time-resolved fluorescence, in situ hybridization, biochip and microflow control technique quantitative checkout equipment at least one.
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