Search Images Maps Play YouTube News Gmail Drive More »
Sign in
Screen reader users: click this link for accessible mode. Accessible mode has the same essential features but works better with your reader.

Patents

  1. Advanced Patent Search
Publication numberCN104677675 A
Publication typeApplication
Application numberCN 201310643371
Publication date3 Jun 2015
Filing date3 Dec 2013
Priority date3 Dec 2013
Publication number201310643371.7, CN 104677675 A, CN 104677675A, CN 201310643371, CN-A-104677675, CN104677675 A, CN104677675A, CN201310643371, CN201310643371.7
Inventors唐寅, 张明, 黄明志, 杭海峰, 易小平, 张建坤, 郭谋之, 何翌清, 曾良平, 金巍巍, 徐奇聪, 张伟平, 马文峰, 张大鹤, 滕小诺, 庄英萍, 张嗣良
Applicant华东理工大学, 上海国强生化工程装备有限公司
Export CitationBiBTeX, EndNote, RefMan
External Links: SIPO, Espacenet
Sampling device and biological reaction device with sampling device
CN 104677675 A
Abstract
The invention discloses a sampling device and a biological reaction device with the sampling device, wherein the sampling device is used for sampling reactants in a bioreactor and comprises a heating cooler, a gas pipeline, a first pinch valve, a second pinch valve, a sampling pipeline, a first peristaltic pump and a second peristaltic pump, wherein the gas pipeline passes through the heating cooler; one end of the gas pipeline is connected to an air filter; the other end of the gas pipeline is an exhaust hole; the first pinch valve is arranged on the gas pipeline and positioned between the air filter and the heating cooler; the second pinch valve is arranged on the gas pipeline and positioned between the heating cooler and the exhaust hole; the sampling pipe passes through the heating cooler; one end of the sampling pipeline is positioned in the bioreactor; the other end of the sampling pipeline is a sample outlet; the first peristaltic pump is arranged on the sampling pipeline and positioned between the bioreactor and the heating cooler; and the second peristaltic pump is arranged on the sampling pipeline and positioned between the heating cooler and the sampler outlet, wherein the parts, passing through the heating cooler, of the gas pipeline and the sampling pipeline are communicated.
Claims(9)  translated from Chinese
1.一种取样装置,用于对生物反应器中的反应物进行取样,其特征在于,包括: 加热冷却器; 气体管道,穿过所述加热冷却器,一端连接于空气过滤器,另一端为排气口; 第一夹管阀,设置于所述气体管道上,且位于所述空气过滤器和第一加热冷却器之间; 第二夹管阀,设置于所述气体管道上,且位于所述第一加热冷却器和排气口之间; 取样管道,穿过所述第一加热冷却器,一端位于生物反应器中,另一端为样品出口; 第一蠕动泵,设置于所述取样管道上,且位于生物反应器和第一加热冷却器之间; 第二蠕动泵,设置于所述取样管道上,且位于第一加热冷却器和样品出口之间; 其中,所述气体管道与所述取样管道穿过所述第一加热冷却器的部分相通。 A sampling device for bioreactor reaction was sampled, characterized in that, comprising: heating a cooler; gas conduit, through said heating cooler one end connected to the air filter, and the other end an exhaust port; a first pinch valve provided on the gas pipeline, and between the air filter and the first heating cooler located; second pinch valve provided on the gas pipeline, and located between the first heating cooler and an exhaust port; sampling line, through the first heating cooler, located at one end of the bioreactor, the other end of the sample outlet; a first peristaltic pump, provided in the the sampling line, and between the bioreactor and the first heating cooler; a second peristaltic pump, disposed in the sampling pipe, and between the first outlet and the sample was heated cooler located; wherein, said gas conduit and the sampling line through the first heating portion communicating cooler.
2.根据权利要求1所述的取样装置,其特征在于,所述气体管道与所述取样管道穿过所述加热冷却器的部分为同一管道。 2. The sampling device according to claim 1, characterized in that said gas conduit and said sampling conduit passes through the heated portion of the same pipe cooler.
3.根据权利要求1所述的取样装置,其特征在于,还包括:第二加热冷却器,设置于所述第二夹管阀与排气口之间; 第三加热冷却器,设置于所述第二蠕动泵与样品出口之间。 3. The sampling device according to claim 1, characterized by further comprising: a second heating cooler, disposed in the second valve interposed between the exhaust outlet pipe; third heating cooler disposed to the said peristaltic pump with the sample between the second outlet.
4.根据权利要求1所述的取样装置,其特征在于,所述管道为柔性硅胶管。 4. The sampling device according to claim 1, characterized in that said conduit is a flexible silicone tube.
5.根据权利要求1所述的取样装置,其特征在于,所述空气过滤器用于对空气进行过滤,向所述管道通入无菌空气。 5. The sampling device according to claim 1, characterized in that said air filter for filtering air to sterile air introduced into the pipe.
6.根据权利要求1所述的取样装置,其特征在于,还包括:控制器,所述控制器包括: 输入输出装置; 中央控制器,与所述输入输出装置连接; 多路继电器,分别连接于所述第一夹管阀、第二夹管阀、第一蠕动泵及第二蠕动泵; 电源模块,与所述多路继电器连接。 6. The sampling device according to claim 1, characterized by further comprising: a controller, said controller comprising: input-output means; the central controller, connected to said input-output means; multiple relays are connected said first pinch valve, the second pinch valve, the first and second peristaltic pump peristaltic pump; power module connected to the multiplexer relays.
7.根据权利要求6所述的取样装置,其特征在于,所述中央控制器为可编程逻辑控制器。 7. The sampling device according to claim 6, characterized in that the central controller is a programmable logic controller.
8.根据权利要求6所述的取样装置,其特征在于,所述输入输出装置为触摸屏。 8. The sampling device according to claim 6, characterized in that said output means is a touch-screen input.
9.一种生物反应装置,其特征在于,包括:生物反应器或分析装置,及与所述生物反应器或分析装置连接的如权利要求1至8中任一取样装置。 A biological reaction apparatus, characterized by comprising: any one of the sampling device 1-8 bioreactor or analysis apparatus, and with the bioreactor or analyzing apparatus as claimed in claim connection.
Description  translated from Chinese
取样装置及具有该取样装置的生物反应装置 Biological sampling device and the sampling device having a reaction apparatus

技术领域 TECHNICAL FIELD

[0001] 本发明涉及生物化学技术领域,且特别涉及一种取样装置及具有该取样装置的生物反应装置。 [0001] The present invention relates to the technical field of biochemistry, and particularly relates to a sampling device and the biological reaction apparatus having a sampling device.

背景技术 Background technique

[0002] 生物反应器是利用酶或生物体(如微生物)所具有的生物功能,在体外进行生化反应的装置系统,它是一种生物功能模拟机,如发酵罐、酶或细胞反应器等。 [0002] The bioreactor is the use of an enzyme or organism (microorganism) has the biological function, in vitro biochemical reaction device system, which is a biological function of the simulator, such as fermenters, an enzyme or cell reactor, etc. . 在酒类、医药生产、浓缩果酱、果汁发酵、有机污染物降解方面有重要应用。 Alcohol, pharmaceutical production, concentrated jam, fruit juice fermentation, degradation of organic pollutants has important applications.

[0003] 在生物反应器的生物培养过程中经常需要进行取样操作。 [0003] In the sampling operation is often required for biological growth process in the bioreactor. 该操作通常要满足无菌、取样量的控制与定量、多次取样的差异控制等方面的要求。 The operation is usually to meet a sterile, sample volume control and quantitative differences between control requirements and other aspects of multiple samples.

[0004] 在现有技术中, [0004] In the prior art,

[0005] 如中国专利,专利申请号:CN200520073106.0,其揭露了一种新型发酵自动取样阀,由液体入口、蒸汽入口、传动机构、取样口及控制阀门组成,其特征在于:在自动取样阀底部设置有带有控制器的伺服电机,蒸汽入口和取样口设在取样阀两侧,一端靠近液体入口,一端位于阀体下部,位于取样阀顶部的液体入口处的接口圆盘面设置了密封环形垫圈。 [0005] The Chinese patent, patent application number: CN200520073106.0, which revealed a novel fermentation automatic sampling valve, the liquid inlet, steam inlet, transmission, sampling port and control valve, is characterized by: automatic sampling the bottom of the valve is provided with a servo motor with controller, steam inlet and sampling ports located on both sides of the sampling valve, near the end of the liquid inlet, located in the lower end of the valve body, the liquid inlet sampling valve located on top of the interface to the disk surface is provided annular gasket seal.

[0006] 还如:如中国专利,专利申请号:CN201020102452.8,其公开了一种发酵罐专用取样阀,包括发酵罐、第一阀和第二阀,所述第一阀的进口与发酵罐相连通,第一阀的出口与第二阀的进口相连通,在该第一阀位于其出口处的阀底座上开设有取样口,在该取样口处安装有取样阀,本实用新型优点是:结构简单,通过在原有阀门的基础之上再安装一阀门,构成阀上阀的结构。 [0006] Also as: such as Chinese patents, patent application number: CN201020102452.8, which discloses a fermentation tank dedicated sampling valve, including the fermenter, the first valve and a second valve, the first valve inlet and Fermentation tank in communication, import export and the second valve is connected through a first valve, opened on the first valve located at the outlet of the valve base has sampling ports, the sampling port sampling valve is installed at the utility model advantages They are: simple structure, through the valve on the basis of the original re-installation of a valve, constituting the valve structure.

[0007] 以上两篇专利的共同特征都是由管道和阀门组件构成的取样装置,能自动、直接取出反应器内部的样品(原样),有蒸气灭菌和密封垫圈,防止取样污染。 Above [0007] are a common feature of both patents by the sampling means components of pipes and valves, can be automatically taken out of the reactor directly inside the sample (as is), there are steam sterilization and sealing gaskets, to prevent sample contamination.

[0008] 如中国专利,专利申请号:CN200910034515.2 “发酵过程在线糖浓度检测系统”,该专利的特征是由恒流泵直接从发酵罐中取样(原样),然后,样品与纯净水按比例混合(稀释)后,送入到检测仪器上测定。 [0008] The Chinese patent, patent application number: CN200910034515.2 "sugar concentration during fermentation line detection system," the patent is characterized sample directly from the fermentor by the constant flow pump (AS), and then press the sample with pure water mixing ratio (dilution), the feeding to the measuring instrumentation. 其采用了恒流泵,不是由阀门的开或关取样。 The use of a constant flow pump, not sampled by the valve on or off. 通过液体的单向流动,避免对反应器(发酵罐)的污染。 By way flow of liquid to avoid contamination of the reactor (fermenter) in.

[0009] 如中国专利,专利申请号:CN02138376.6“一种生物反应过程在线检测系统的取样稀释装置”该专利在取样探头末端(与发酵罐连接)放置一陶瓷膜过滤件,反应器内的液体在膜件中连续的过滤,将微量的过滤液由管道引出(不是原样),经过一个稀释装置后,进入检测仪测定。 [0009] The Chinese patent, patent application number: "sample dilution means a biological reaction process online testing system" CN02138376.6 the patent at the end of the sampling probe (with fermenter connection) placed a ceramic membrane filter elements, reactor The liquid continuous filtration membrane elements, the small amount of filtered liquid drawn from the pipeline (not as), after a dilution means to enter the detector measurement. 陶瓷膜孔径在0.1〜0.2pm之间,微生物不能通过,能有效防止反应器(发酵罐)污染。 Ceramic membrane pore size between 0.1~0.2pm, microorganisms can not pass, can effectively prevent the reactor (fermenter) contamination.

[0010] 如美国专利,专利号US4,942,770,Selfert “一种无菌取样装置”。 [0010] The US Patent No. US4,942,770, Selfert "a sterile sampling device." 由一个三通阀与反应器连接,通过变换阀门通路完成取样,即先与发酵罐接通,(正向)流入取样管道,然后变换阀门(关闭与发醉罐内部的联通,开启与外部管道的联通),将取样管道内的样品(原样)由液体泵反向送入下一步的稀释装置或检测仪器。 Connected by a three-way valve and the reactor is completed sampling by changing the valve passage, that is, first and fermentation tank connected, (forward) into the sampling line, then converting valve (closed and hair drunk tank internal Unicom, opening and external piping Unicom), the sample (AS) pipeline by sampling liquid pump reverse into the next dilution device or instrumentation. 由于三通阀的切换,避免了反应器内部与外界的直接接触,从而起到无污染取样的效果。 Since the switching three-way valve, to avoid direct contact with the interior of the reactor and the outside world, and thus play a clean sample results.

[0011] 如美国专利,专利号US5,409,841,Chow “紫外线灭菌的取样装置和方法”,采用紫外灯对取样管道和容器进行灭菌,不采用蒸汽灭菌。 [0011] The US Patent No. US5,409,841, Chow "sampling device and method of ultraviolet sterilization", using ultraviolet light to sterilize the sampling pipes and containers, do not use steam sterilization.

[0012] 如美国专利,专利号US4,695,551,Samhaber,“生物反应器取样装置”,采用半透膜渗透过滤取样。 [0012] The US Patent No. US4,695,551, Samhaber, "bioreactor sampling device", the use of semi-permeable membrane permeation filter sampling. 半透膜可隔离微生物通过,避免污染,同时增加一个半透膜震动装置,防止膜孔的阻塞。 Semipermeable membrane can isolate microbes through, avoiding pollution, while increasing a semipermeable membrane vibration device to prevent blockage of membrane pores.

[0013] 如美国专利,专利号”5,914,092^0011,“发酵罐取样阀门蒸汽灭菌装置”,涉及一种关于提高蒸汽对取样阀门和管道灭菌效率的装置和方法。 [0013] The US Patent No. "5,914,092 ^ 0011," fermenter sampling valve steam sterilization device ", relates to an apparatus and method of steam on improving the sampling valves and pipe sterilization efficiency.

[0014] 如美国专利,专利号US6,860,162,Jaeger,“液体取样和方法”,在葡萄酒发酵罐取样时,设计了一个沉淀装置,取样探头可以避开发酵液中沉淀物,防止阻塞。 [0014] The US Patent No. US6,860,162, Jaeger, "liquid sampling and methods" in the wine fermentation tank sampling, the design of a sedimentation device, the sampling probe can avoid precipitate broth, prevent obstruction .

[0015] 综上所述,现有技术中的生物反应中的取样操作主要有两种类型,一类是分离膜过滤,一类是由管道和阀门组成。 [0015] In summary, the prior art bioreactors sampling operation in two main types, one is a separation membrane filter, one is by the pipes and valves.

[0016] 然而以上取样操作方法存在以下缺陷及不足,具体如下: [0016] However, there are more than sampling methods of operation following defects and deficiencies, as follows:

[0017] 分离膜过滤取样:由于膜的孔径阻止了菌体的通过,避免了外源污染。 [0017] a separation membrane filter sampling: As pore size prevents bacteria by avoiding the exogenous pollution. 但对于需要检测培养液中固形物含量、某些胞内组分的检测、对生物进行镜检等实验要求,这种方式不能适用。 But for the need to detect the culture medium solids content, detect certain intracellular components of the microscopic organisms and other test requirements, this approach can not be applied.

[0018] 管道与阀门组成的取样装置:能满足上述实验检测的需要。 [0018] The sampling device consisting of pipes and valves: to meet the needs of the above experimental detection. 同时也存在以下局限: There is also the following limitations:

[0019] 一、对防止取样造成污染的控制方式: [0019] First, to prevent pollution of control samples:

[0020] 1、环境控制加消毒剂(酒精)对接口进行消毒:对使用环境要求高,消毒剂对取出样品有干扰(组分、镜检形态等); [0020] 1, environmental control plus disinfectant (alcohol) to interface disinfection: the use of environmental requirements, disinfectants to remove the sample interference (component, microscopic morphology, etc.);

[0021] 2、蒸汽对管路与接口进行消毒:使用时需要配置蒸汽,对管道、实验装置要求复杂(耐压、防烫伤、蒸汽排放),适用于较大的金属反应装置; [0021] 2, the steam pipe and the interface disinfect: you need to configure the use of steam, complex pipeline, laboratory equipment requirements (pressure, anti-scald, steam emissions), applicable to large metal reaction apparatus;

[0022] 3、紫外线消毒法:给操作带来复杂性,且对活性微生物的取样带来无法评估的影响。 [0022] 3, ultraviolet disinfection method: to bring the complexity of the operation, and the impact can not be assessed on the activity of microorganisms sampled.

[0023] 4、三通切换阀方式:生物培养一般采用隔膜阀,三通阀结构存在死角,无法评估造成污染的机会。 [0023] 4, three-way switching valve way: biological culture generally use diaphragm valve, three-way valve structure exists dead, unable to assess the chance of pollution.

[0024] 二、对取样过程废液排放量的控制: [0024] Second, the sampling process waste emissions control:

[0025] 在取样过程中,需要先将管道中残存废液排除,才能使取出样液能够与培养装置中的培养液一致。 [0025] In the sampling process, we need to exclude pipeline residual waste, in order to make the sample solution can be taken in line with the broth culture device. 微生物培养是个缓慢的过程,有的要一天以上,有的要许多天。 Microbial culture is a slow process, and some want more than one day, some to many days. 如果在整个过程中取样次数较多,而培养液总体积又有限的情况下,对每次取样时管道残存废液排放量的控制就尤为重要。 If the sample more frequently throughout the process, while the total volume of the culture medium and under limited circumstances, for every time the sampling line residual waste emissions control is particularly important.

[0026]由上可知,现有技术中的生物培养过程中的取样装置,无法满足严格无菌、取样量的控制与定量、多次取样的差异控制等方面的要求。 [0026] From the foregoing, biological cultivation process of the prior art in the sampling device, unable to meet the stringent sterile sample volume control and quantitative differences between control requirements and other aspects of multiple samples.

发明内容 SUMMARY OF THE INVENTION

[0027] 有鉴于此,本发明旨在解决现有技术中的生物培养过程中的取样装置,无法满足严格无菌、取样量的控制与定量、多次取样的差异控制等方面的要求等技术问题。 [0027] In view of this, the present invention aims to solve biological cultivation process of the prior art in the sampling device, unable to meet the stringent sterile sample volume control and quantitative differences between multiple sampling control technology requirements problem.

[0028] 为解决上述问题,本发明提供一种取样装置,用于对生物反应器中的反应物进行取样,包括:加热冷却器;气体管道,穿过所述加热冷却器,一端连接于空气过滤器,另一端为排气口;第一夹管阀,设置于所述气体管道上,且位于所述空气过滤器和加热冷却器之间;第二夹管阀,设置于所述气体管道上,且位于所述加热冷却器和排气口之间;取样管道,穿过所述加热冷却器,一端位于生物反应器中,另一端为样品出口;第一蠕动泵,设置于所述取样管道上,且位于生物反应器和加热冷却器之间;第二蠕动泵,设置于所述取样管道上,且位于加热冷却器和样品出口之间;其中,所述气体管道与所述取样管道穿过所述加热冷却器的部分相通。 [0028] In order to solve the above problems, the present invention provides a sampling device for the reaction in the bioreactor was sampled, comprising: heating a cooler; gas conduit, through said heating cooler one end connected to the air filter, and the other end of the exhaust port; a first pinch valve provided on the gas pipeline, and between the air filter and heating cooler located; second pinch valve provided in the gas pipe on, and is located between the cooler and the heated exhaust port; sampling line, passing through the heating condenser, located at one end of the bioreactor, the other end of the sample outlet; a first peristaltic pump, disposed in said sampling the pipe, and between the bioreactor and the heating cooler; a second peristaltic pump, disposed in the sampling pipe, and is located between the cooler and the heated sample outlet; wherein, said gas conduit and said sampling conduit cooler through the heating section connected.

[0029] 进一步的,所述气体管道与所述取样管道穿过所述加热冷却器的部分为同一管道。 [0029] Further, the gas pipeline and the sampling line passes through the heating cooler part of the same pipe.

[0030] 进一步的,所述的取样装置,还包括:第二加热冷却器,设置于所述第二夹管阀与排气口之间;第三加热冷却器,设置于所述第二蠕动泵与样品出口之间。 [0030] Further, the sampling apparatus further comprises: a second heating cooler, disposed between the second pinch valve and an exhaust port; third heating cooler, disposed in said second creep between the pump and the sample outlet.

[0031 ] 进一步的,所述管道为柔性硅胶管。 [0031] Further, the pipe is a flexible silicone tube.

[0032] 进一步的,所述空气过滤器用于对空气进行过滤,向所述管道通入无菌空气。 [0032] Further, the air filter for filtering air to sterile air introduced into the pipe.

[0033] 进一步的,所述取样装置还包括控制器,所述控制器包括:输入输出装置;中央控制器,与所述输入输出装置连接;多路继电器,分别连接于所述第一夹管阀、第二夹管阀、第一蠕动泵及第二蠕动泵;电源模块,与所述多路继电器连接。 [0033] Further, the sampling apparatus further comprises a controller, the controller comprising: input-output means; the central controller, and output means connected to said input; multiple relays respectively connected to the first tube clamp valve, the second pinch valve, the first and second peristaltic pump peristaltic pump; power module connected to the multiplexer relays.

[0034] 进一步的,所述中央控制器为可编程逻辑控制器。 [0034] Further, the central controller is a programmable logic controller.

[0035] 进一步的,所述输入输出装置为触摸屏。 [0035] Further, the input-output device as a touch screen.

[0036] 本发明还提供一种生物反应装置,其包括:生物反应器或分析装置,及与所述生物反应器或分析装置连接的如上所述任一取样装置。 [0036] The present invention further provides a biological reaction apparatus, comprising: a sampling device as described above according to any bioreactor or analysis apparatus, and with the bioreactor or analyzing apparatus connected.

[0037] 综上所述,本发明提供的取样装置及具有该取样装置的生物反应装置,由于采用无菌空气保压,并借助上述取样管路上各执行器件来保证无菌状态的前提下,能取出活体生物进行分析与镜检,同时避免了生物形态所受高温干扰的情况。 [0037] In summary, the present invention provides a sampling device and biological reaction apparatus having the sampling device, the use of sterile air packing, and means of implementing the above sample line under the premise of the device to ensure sterility, living organisms can be taken out for analysis and microscopic examination, while avoiding the situation biomorphic suffered high temperature interference.

[0038] 其次,由于管道中采用无菌空气进行保压,即可通过蠕动泵的反转,用无菌空气将插入被取样液体中胶管内的残留液体反吹回被取样液体中,既避免了残留液体对实时取样分析结果的干扰,也使通常所需的残留废液排出量降为零;同时依靠蠕动泵正转的时间与流量的预先设定,使每次取样量能实现基于蠕动泵精度的定量化。 [0038] Second, because the pipeline packing sterile air can be reversed by a peristaltic pump, with sterile air will be inserted into the sampling residual liquid inside the cleaning liquid in the hose back to the liquid to be sampled, both to avoid the interference of the real-time sampling residual liquid analysis results, also the residual waste discharge amount normally required zero; while relying on forward peristaltic pump and flow pre-set time, so that the amount of energy each sampling-based motility Pump accuracy quantified.

[0039] 最后,由于通过控制器的程序设定的取样操作步骤,能够使取样操作配合外接自动分析仪进行自动取样并在线分析,也能利用手动取样模式进行临时取样离线分析。 [0039] Finally, since the program is set by the controller sampling procedure, the sampling operation can be automated analyzer with an external automatic sampling and on-line analysis, utilizing manual sampling mode can be temporarily offline sample analysis.

附图说明 Brief Description

[0040] 图1所示为本发明一实施例提供的取样装置的结构示意图;图2所示为本发明一实施例提供的取样装置的结构示意图; [0040] Figure 1 shows a schematic diagram of the structure of the present invention to provide a sampling device according to an embodiment; FIG. 2 is a schematic diagram illustrating the structure of the invention provides an embodiment of the sampling device;

[0041] 图3所示为本发明另一实施例提供的取样装置的结构示意图; It provides a schematic illustration of the structure of the sampling device according to another embodiment of the present invention shown in [0041] Figure 3;

[0042] 图4所示为本发明另一实施例提供的取样装置的结构示意图; It provides a schematic illustration of the structure of the sampling device according to another embodiment [0042] Figure 4 shows the present invention;

[0043] 图5所示为本发明另一实施例提供的取样装置的结构示意图; It provides a schematic illustration of the structure of the sampling device according to another embodiment [0043] Figure 5 shows the present invention;

[0044] 图6所示为本发明另一实施例提供的取样装置的结构示意图。 [0044] Figure 6 shows a schematic diagram of the structure of the present invention to provide a sampling device according to another embodiment.

具体实施方式 DETAILED DESCRIPTION

[0045] 为使本发明的目的、特征更明显易懂,下面结合附图对本发明的具体实施方式作进一步的说明。 [0045] For the purposes of this invention, it features more fully understood with reference to the following specific embodiments of the present invention will be further described.

[0046] 实施例一 [0046] Embodiment 1

[0047] 请参见图1,其所示为为本发明一实施例提供的取样装置的结构示意图。 [0047] See Figure 1, which shows the schematic structure of the present invention to provide a sampling apparatus according to an embodiment of the.

[0048] 该取样装置,用于对生物反应器中的反应物进行取样,包括:加热冷却器130 ;气体管道121,穿过所述加热冷却器130, —端连接于空气过滤器110,另一端为排气口;第一夹管阀141,设置于所述气体管道上,且位于所述空气过滤器110和加热冷却器130之间;第二夹管阀142,设置于所述气体管道上,且位于所述加热冷却器130和排气口之间;取样管道122,穿过所述加热冷却器130,一端位于生物反应器100中,另一端为样品出口;第一蠕动泵151,设置于所述取样管道122上,且位于生物反应器100和加热冷却器130之间;第二蠕动泵152,设置于所述取样管道122上,且位于加热冷却器和样品出口之间;其中,所述气体管道121与所述取样管道122穿过所述加热冷却器130的部分相通。 [0048] The sampling means, for a reaction in the bioreactor was sampled, comprising: heating a cooler 130; a gas conduit 121, the cooler 130 through the heating, - end is connected to the air filter 110, and the other One end of exhaust port; a first pinch valve 141, provided on the gas pipe and located between the air filter 130 and the heating cooler 110; a second pinch valve 142, disposed in the gas conduit on, and is located between the cooler 130 and an exhaust port of said heating; sampling line 122, cooler 130 through the heating, the bioreactor is located at one end 100, the other end of the sample outlet; a first peristaltic pump 151, disposed in the sampling pipe 122, and between 100 and 130 bioreactor heated cooler; a second peristaltic pump 152, disposed in the sampling pipe 122, and is located between the cooler and the heated sample outlet; wherein , the gas conduit 121 and the conduit 122 through the sampling portion 130 of the cooler is heated in communication.

[0049] 在本发明实施例中,所述气体管道121与所述取样管道122穿过所述加热冷却器130的部分为同一管道。 [0049] In the present embodiment of the invention, the gas conduit 121 and the sampling line 122 cooler 130 through the heating portion of the same pipe.

[0050] 在本发明实施例中,所述气体管道121与所述取样管道122为柔性硅胶管。 [0050] In the embodiment of the present invention, the gas conduit 121 and the sampling line 122 is a flexible silicone tube.

[0051] 利用本发明实施例提供的取样装置进行取样的具体步骤如下: [0051] The present invention is the use of specific steps according to an embodiment of sampling apparatus for sampling as follows:

[0052] 一、对防止取样造成污染的控制: [0052] First, the prevention of pollution control sample:

[0053] 1、该装置所有器件均采用夹管方式,例如:夹管阀、蠕动泵、加热与冷却器,使夹管阀、蠕动泵、加热与冷却器等器件与被取样无直接接触。 [0053] 1, this means that all devices are pinch, for example: pinch valve, peristaltic pump, heating and cooling, so pinch valves, peristaltic pumps, heating and cooling and other devices without direct contact with the sample.

[0054] 2、所述气体管道121与所述取样管道122采用柔性硅胶管,所述气体管道121上配有空气过滤器110,所述取样管道122与培养装置,如生物反应器100,实现连接就位,所述气体管道121与所述取样管道122与外界接口夹死或无菌包扎。 [0054] 2, the gas conduit 121 and the sampling line 122 with flexible silicone tube, said gas pipe 121 is equipped with an air filter 110, the sampling pipe 122 and the culture device, such as a biological reactor 100, to achieve connection in place, the gas conduit 121 and the sampling pipe 122 interfaces with the outside world to death or sterile dressing. 使用前与培养装置一起放入高压灭菌锅内灭菌。 Before using the device in conjunction with the culture pot into the autoclave sterilization. 使用时只需在培养过程本身所需环境条件下操作,将管道卡入夹管阀、螺动泵、加热冷却器。 Simply use in the training process itself needed to operate under ambient conditions, the pipeline snap pinch valve, screw acting pump, heating cooler. 由于第二夹管阀142、第二螺动泵152已将取样管道122夹死,此时放开所述气体管道121与所述取样管道122与外界接口进行连接时,不会产生连接过程对培养液的污染。 Since the second pinch valve 142, the second movable screw pump 152 has sampling line 122 to death, then release the gas conduit 121 and conduit 122 when the sampling interface to connect with the outside world, no connection processes culture contaminated fluid.

[0055] 3、第二夹管阀142、第二蠕动泵152后的取样管道122与外界的非无菌管道的连接,在长时间培养过程中可能造成杂菌越过第二夹管阀142、第二蠕动泵152造成对培养装置内的污染,采用无菌空气(压缩空气经上述空气过滤器过滤)对系统进行无菌保压、取样完毕打开第二夹管阀142将粘附于管道壁上的液滴吹掉;同时根据巴士德消毒原理,利用加热冷却器130对取样管道122进行热保温,以杜绝杂菌沿管道逆向繁殖、污染培养装置内部。 [0055] 3, the second pinch valve 142, sampling line 152 after the second peristaltic pump 122 is connected with a non-sterile outside of the pipe, in the long training process can cause bacteria over the second pinch valve 142, second peristaltic pump 152 causing pollution within the device culture, sterile air (compressed air through the air filter) system for aseptic packing, sampling is completed to open the second pinch valve 142 will adhere to the pipe wall the droplets to blow off; at the same time according to the principle of pasteurized by heating cooler 130 pairs of sampling line 122 for heat insulation, in order to eliminate the bacteria along the pipeline reverse breeding, cultivation of pollution inside the apparatus. 如取出样品经过这段高温管道可能对组分、镜检外形等产生影响,可在取样时通过将加热模式切换为冷却模式对管道进行降温。 As a sample was taken may have an impact on the component, microscopic appearance, etc. After this heat pipe can be sampled at the time by the heating mode is switched to the cooling mode to cool the pipeline.

[0056] 二、对取样过程废液排放量的控制: [0056] Second, the sampling process waste emissions control:

[0057] 1、由于采取了上述无菌控制措施,保证了管道内部的无菌状态,在取样前打开第一夹管阀141,通过第一蠕动泵151反转,如逆时针旋转,将培养装置中取样管道122内的残存液体打回取样装置内。 [0057] 1. As a result of the above sterile control measures to ensure the sterility of the interior of the pipe before the sampling opening the first pinch valve 141, 151 through the first reverse peristaltic pump, such as rotating counterclockwise, the culture apparatus remaining liquid sampling pipe 122 to play back the sample in the device. 再使第一蠕动泵151、第二蠕动泵152正转,如顺时针旋转,此时取出的样品为即时样,而不用排出废液。 Then the first peristaltic pump 151, a second peristaltic pump 152 is transferred, such as rotating clockwise, the samples taken at this time is real - like, instead of discharging waste.

[0058] 2、根据取样量需求,通过对蠕动泵转速、管道内径、蠕动泵两端压差等状态确定下对应蠕动泵流量的标定,即可通过蠕动泵的运转时间的设定,控制取样量。 [0058] 2. The sample volume demand, by a peristaltic pump speed, pipe diameter, peristaltic pump pressure at both ends and other status determination under the corresponding peristaltic pump flow calibration can be set by a peristaltic pump operating time, the control sample the amount. 当达到所需取样量时,使第一蠕动泵151反转,如逆时针旋转,第二蠕动泵152继续正转,如顺时针旋转。 When the desired sample volume, the first reverse peristaltic pump 151, such as rotating counterclockwise, second peristaltic pump 152 to continue forward, such as rotating clockwise. 此时以无菌空气管道接入点为分界点,近第一蠕动泵151端的残余液体被完全压入培养装置,近第二蠕动泵152端的取出样在无菌空气的顶推下完全流入外接取样装置或分析仪,整个过程不会产生采样过量与废液。 This time with sterile air duct access point as a dividing point, near the first end of the peristaltic pump residual liquid 151 is completely pressed into the culture device, remove the side of the nearly 152 samples in a second peristaltic pump pushing under completely sterile air flows into the external sampling means or analyzer, the entire process does not produce excessive sampling and waste.

[0059] 在本发明实施例中,请参见图2,该取样装置还包括控制器200,所述控制器200包括:输入输出装置210 ;中央控制器220,与所述输入输出装置210连接;多路继电器230,分别连接于所述第一夹管阀、第二夹管阀、第一蠕动泵及第二蠕动泵;电源模块240,与所述多路继电器230连接。 [0059] In the embodiment of the present invention, see Figure 2, the sampling apparatus further comprises a controller 200, the controller 200 includes: input-output means 210; the central controller 220, 210 is connected to the input-output device; Multiple relay 230, respectively connected to the first pinch valve, the second pinch valve, a first peristaltic pump and the second peristaltic pump; power module 240, the relay 230 is connected to the multiplexer.

[0060] 在本发明实施例中,所述中央控制器可为可编程逻辑控制器或单片机。 [0060] In embodiments of the invention, the central controller may be a programmable logic controller or microcontroller.

[0061 ] 在本发明实施例中,所述输入输出装置为触摸屏。 [0061] In embodiments of the invention, the input-output device is a touch screen.

[0062] 基于上述管道、执行器件的结构简洁,控制器配以程序化控制,可以做到采样时间点、采样过程时间、采样量的重复性控制。 [0062] Based on the structure of the above-mentioned pipeline, the implementation of the device is simple and the controller with programmed control, you can do the sampling time point, the sampling process time, sample volume repeatability control. 尤其对长周期、多次采样的情况,避免了人工操作带来的误差,对培养液总量有限的情况也能满足使用要求,从而满足了对采样操作重复性的控制要求。 Especially for a long period, the case of multiple samples, avoiding errors caused by manual operation, for a limited amount of liquid in the case of culture can meet the requirements to meet the sampling operation for repetitive control requirements.

[0063] 实施例二 [0063] Second Embodiment

[0064] 请参见图3,其所示为本发明另一实施例提供的取样装置的结构示意图。 [0064] Refer to Figure 3, the illustrated embodiment of the present invention to provide structural diagram of the sampling device according to another embodiment.

[0065] 在本实施例中,所述的取样装置,包括:第一加热冷却器331 ;气体管道321,穿过所述第一加热冷却器331, —端连接于空气过滤器310,另一端为排气口323 ;第一夹管阀341,设置于所述气体管道上,且位于所述空气过滤器310和第一加热冷却器331之间;第二夹管阀342,设置于所述气体管道上,且位于所述第一加热冷却器331和排气口323之间;取样管道322,穿过所述第一加热冷却器331, —端位于生物反应器300中,另一端为样品出口324 ;第一蠕动泵351,设置于所述取样管道322上,且位于生物反应器300和第一加热冷却器331之间;第二蠕动泵352,设置于所述取样管道322上,且位于第一加热冷却器331和样品出口324之间;其中,所述气体管道121与所述取样管道122穿过所述加热冷却器130的部分相通。 [0065] In the present embodiment, the sampling device, comprising: a first heating cooler 331; gas duct 321, cooler 331 through the first heating, - end is connected to the air filter 310, and the other end the exhaust port 323; the first pinch valve 341, provided on the gas pipe, and between the air filter 310 and the cooler 331 is located in the first heating; second pinch valve 342, disposed at the the gas pipe and located between the first heating cooler 331 and an exhaust port 323; sampling line 322, cooler 331 through the first heating, - end is positioned in the bioreactor 300, the other end of the sample outlet 324; a first peristaltic pump 351, disposed in the sampling pipe 322, and is located between the bio-filter 300 and the first reaction was heated cooler 331; a second peristaltic pump 352, disposed in the sampling pipe 322, and heating the cooler 331 in the first outlet 324 and the sample; wherein the gas conduit 121 and the conduit 122 through the sampling portion 130 of the cooler is heated in communication. 所述还包括:第二加热冷却器332,设置于所述第二夹管阀与排气口之间;第三加热冷却器333,设置于所述第二蠕动泵352与样品出口之间。 It said further comprising: a second heating cooler 332, disposed in the second valve interposed between the exhaust port and the duct; third heating cooler 333, disposed between the peristaltic pump 352 and the second sample outlet.

[0066] 在本实施例中,其中加热冷却器331、332、333可根据与分析装置的远近进行调整,可采用一个第一加热冷却器331,也可采用第二及第三加热冷却器332、333对两根管道各自控温,或将332、333组合成一个双通道模块。 [0066] In the present embodiment, wherein heating the cooler 331, 332 can be adjusted according to the distance and analysis means, cooler 331 may be a first heating, second and third heating may also be employed cooler 332 , 333 pairs of two tubes each temperature, or 332, 333 combined into a dual-channel module. 本发明并非局限于此。 The present invention is not limited thereto.

[0067] 实施例三 [0067] Third Embodiment

[0068] 请参见图4,其所示为本发明另一实施例提供的取样装置的结构示意图。 [0068] See Figure 4, which illustrated embodiment of the present invention to provide a structural diagram of another embodiment of the sampling device.

[0069] 与实施例一相比,实施例一中的蠕动泵151在实施例三中用夹管阀442替代,相应地对反应器内液体的抽拍动力改由注射泵451提供。 [0069] Compared with one embodiment, the embodiment of a peristaltic pump 151 in the third example of using an alternative pinch valve 442, corresponding to the liquid pumping beat dynamic change within the reactor 451 is provided by the injection pump.

[0070] 实施例三的取样执行器件,由注射泵451、蠕动泵452,夹管阀441、442、443,储液管470,加热冷却器431或432、433,柔性硅胶管及管道连接件组成,再与控制模块组合成整个取样装置。 [0070] The sampling device according to the three execution by injection pump 451, peristaltic pump 452, pinch valves 441,442,443, liquid pipe 470, 431 or 432, 433 heating cooler, flexible silicone tube and pipe fittings composition, and then combined with the control module to the entire sampling device.

[0071] 根据所需的取样精度,注射泵451也可用精度较低的蠕动泵替代(因二者功能不同,具体程控步骤见流程3及其描述)。 [0071] Depending on the desired accuracy of sampling, the syringe pump 451 may also be less accurate peristaltic pump replacement (due to the two different functions, depending programmable steps, see Scheme 3 and described below).

[0072] 使用前将排空胶管422、样品胶管423包扎。 [0072] Before using the emptying hose 422, 423 sample hose wrap. 将储液管470、生物反应器400、过滤器410及之后的胶管按图连接后一起放入高压灭菌锅灭菌。 The reservoir tube 470, bioreactor 400, hose and filter after 410 sterilized in an autoclave together into the press view of the connector. 灭菌完成后按图示将胶管装入各执行器,并将空气滤器的进口与空气管421连接。 Press the icon will be charged hose after sterilization is completed for each actuator and the air filter and the air inlet pipe 421 is connected.

[0073] 采用实施例三提供的取样装置进行操作的具体流程如下: Specific processes sampling device provided in the third embodiment [0073] The operation was carried out as follows:

[0074] 1、反应器内取样管排残余液: [0074] 1, the reactor discharge residual liquid sampling tube:

[0075] 打开夹管阀441、蠕动泵抽气至合适体积;闭夹管阀441,打开夹管阀442,注射泵451压出气体,将反应器取样管内残存液体压回生物反应器内。 [0075] Open pinch valve 441, the peristaltic pump exhaust to an appropriate volume; closed pinch valve 441, open pinch valve 442, the syringe pump 451 out of gas pressure, the reactor pressure sampling tube remaining liquid back into the bioreactor. 开启加热冷却器的冷却功能以降低其中管道的温度。 Open heating cooler cooling function to reduce the temperature of the pipe where.

[0076] 2、抽液: [0076] 2, pumping fluid:

[0077] 注射泵451抽气,将反应器内液体按需要量定量抽出至储液管470。 [0077] The syringe pump 451 pumping the liquid reactor according to the requirements for quantitative extraction reservoir tube 470.

[0078] 3、出样: [0078] 3, sampling:

[0079] 关闭夹管阀442 ;打开夹管阀141,同时螺动泵452逆时针旋转,将液体压入分析装置或取样容器。 [0079] Close pinch valve 442; open pinch valve 141, while moving the pump screw 452 rotates counterclockwise, the liquid into the analysis device or sampling container.

[0080] 4、吹气: [0080] 4, inflatable:

[0081] 关闭蠕动泵452 ;打开夹管阀443并迅速关闭,吹出粘附在管壁上的微量液体,以减小对下次取样的干扰。 [0081] Close peristaltic pump 452; pinch valve 443 open and close quickly blown micro liquid adhered on the wall, to reduce interference to the next sample. 开启加热冷却器的加热功能。 Open heating cooler heating.

[0082] 5、压回取样管残余液体: [0082] 5, pushed back the residual liquid sampling tube:

[0083] 重复步骤I。 [0083] Repeat Step I.

[0084] 步骤I与步骤5重复,功能一致。 [0084] Step I and Step 5 Repeat, consistent function. 只是在第一次取样时需要步骤1,之后每次操作只是步骤2-5的循环。 Just in the first sampling required Step 1, Step 2-5 just after the circulation of each operation.

[0085] 实施例四 [0085] Fourth Embodiment

[0086] 请参见图5,其所示为本发明另一实施例提供的取样装置的结构示意图。 [0086] Refer to Figure 5, the illustrated embodiment of the present invention to provide structural diagram of the sampling device according to another embodiment.

[0087] 与实施例三相比,主要区别在于:注射泵451用实施例四中的蠕动泵551替换。 [0087] Compared with the third embodiment, the main difference is: injection pump 451 with four cases in the alternative embodiment of the peristaltic pump 551.

[0088] 运行时分以下步骤执行: [0088] Running Time the following steps:

[0089] 1、反应器内取样管排残余液: [0089] 1, the reactor discharge residual liquid sampling tube:

[0090] 打开夹管阀542,同时蠕动泵551逆时针旋转,用无菌空气将反应器取样管内参与液体压回反应器内。 [0090] Open pinch valve 542, while the peristaltic pump 551 rotates counterclockwise, with sterile air inside the reactor involved in the sampling tube pressure of the liquid back into the reactor. 开启加热冷却器的冷却功能以降低其中管道的温度。 Open heating cooler cooling function to reduce the temperature of the pipe where.

[0091] 2、抽液: [0091] 2, pumping fluid:

[0092] 蠕动泵551顺时针旋转,将反应器内液体按需要量定量抽出至储液管170。 [0092] Peristaltic pump 551 rotates clockwise by the required amount of liquid in the reactor to quantitatively extract the reservoir tube 170.

[0093] 3、出样: [0093] 3, sampling:

[0094] 关闭夹管阀542 ;打开夹管阀541,同时蠕动泵552逆时针旋转,将液体压入分析装置或取样容器。 [0094] Close pinch valve 542; open pinch valve 541, while the peristaltic pump 552 rotates counterclockwise, the liquid into the analysis device or sampling container.

[0095] 4、吹气: [0095] 4, inflatable:

[0096] 关闭蠕动泵552 ;打开夹管阀543并迅速关闭,吹出粘附在管壁上的微量液体,以减小对下次取样的干扰。 [0096] Close peristaltic pump 552; pinch valve 543 open and close quickly blown adhesion trace liquid on the wall, to reduce interference to the next sample. 开启加热冷却器的加热功能。 Open heating cooler heating.

[0097] 5、压回取样管残余液体: [0097] 5, pushed back the residual liquid sampling tube:

[0098] 重复步骤I。 [0098] Repeat Step I.

[0099] 步骤I与步骤5重复,功能一致。 [0099] Step I and Step 5 Repeat, consistent function. 只是在第一次取样时需要步骤1,之后每次操作只是步骤2-5的循环。 Just in the first sampling required Step 1, Step 2-5 just after the circulation of each operation.

[0100] 对上述实施例二至四,还可进行变化组合,包括但不限于: [0100] The above embodiments two to four, but also for changes in composition, including, but not limited to:

[0101]根据取样量的体积,确定储液管的容积,或直接由管道长度替代储液管容积。 [0101] According to the amount of sample volume, determine the volume of the reservoir tube or pipe replacement reservoir volume directly from the pipe length.

[0102]根据取样装置与分析装置的距离,加热冷却器可以是单独的,也可在排气管道和出样管道单独设置或组合而成双通道的加热冷却模块。 [0102] According to distance sampling device and analysis device, heating cooler may be a separate, it can also be provided separately or a combination of dual channel heating and cooling module in the exhaust duct and sampling pipes.

[0103]根据具体使用操作需求,夹管阀可用蠕动泵替换,蠕动泵也可用夹管阀替换。 [0103] Depending on the use of operational requirements, pinch valves can be used to replace the peristaltic pump, a peristaltic pump can also be used pinch valve replacement.

[0104] 将夹管阀、夹管阀换成蠕动泵,程控步骤不变,只涉及夹管阀的开关控制与蠕动泵的旋转控制(包括旋转方向控制)。 [0104] The pinch valves, pinch valves replaced with peristaltic pumps, programmable step unchanged, only relates to a rotary control switch control pinch valves and peristaltic pump (including the rotation direction control).

[0105] 实施例五 [0105] Embodiment 5

[0106] 请参见图6,其所示为本发明另一实施例提供的取样装置的结构示意图。 [0106] See Figure 6, which shown a schematic diagram of the structure of the present invention to provide a sampling device according to another embodiment.

[0107] 在本实施例中,该取样装置除了与实施例中的反应器组合后一并在高压灭菌锅中灭菌的应用方式外,也可采用将该装置与反应器的连接管采用隔膜阀连接的方式,见图6。 [0107] In the present embodiment, the sampling means in addition to the post-reactor embodiment be implemented in a combination of an autoclave sterilized external application mode, the device may also be used in connection with the reactor tube with way diaphragm valve connected as shown in Figure 6.

[0108] 隔膜阀682、683焊接连接。 [0108] Diaphragm 682,683 soldered connections. 使用前隔膜阀682关闭,与胶管、空气滤器610、储液管670连接,将排气管623、出样管624包扎后,一并放入高压灭菌锅中灭菌。 Before using the diaphragm valve 682 is closed, the connection with the hose, air filter 610, the reservoir tube 670, the exhaust pipe 623, 624 of the sample tube after dressing together into an autoclave sterilization.

[0109] 使用时,将隔膜阀682与反应器取样阀684的接口连接,采用生物反应器的常规二次蒸汽灭菌的连接方式,实现生物反应器与取样装置的无菌连接(此处不再赘述)。 [0109] In use, the diaphragm 682 and the valve reactor sampling interface 684, connection using a conventional bioreactor secondary steam sterilization, the bioreactor to achieve a sterile connection with the sampling device (here not to repeat).

[0110] 无菌连接后,将隔膜阀682打开、隔膜阀681、683关闭,之后的自动取样的程控步骤与前述流程与步骤一致。 [0110] After the sterile connection, the diaphragm 682 is open, the diaphragm valve 681,683 is closed after the programmed automatic sampling step is consistent with the aforementioned processes and steps. 隔膜阀681-683、取样阀684可以是手动阀,也可以是自控阀,其自控程序也可整合到取样控制器中。 Diaphragm valves 681-683, sampling valve 684 may be a manual valve, a valve may be controlled, which may also be integrated into the program-controlled sampling controller.

[0111] 此外,本发明实施例还提供了一种生物反应装置,其包括:生物反应器或分析装置,及与所述生物反应器或分析装置连接的如上述实施例提供的任一取样装置。 [0111] In addition, embodiments of the present invention further provides a biological reaction apparatus, comprising: a sampling device as described in any one of embodiments bioreactor or analysis apparatus, and with the bioreactor or analyzing means connected to provide .

[0112] 例如:整套生物反应装置可与生物反应器组合而成,也可与分析装置组合使用,或三者一并组合使用。 [0112] For example: the entire biological reaction apparatus may be a combination of the bioreactor, and analysis means may also be used in combination, or all three together in combination. 其具体结构原理如上,在此不再赘述。 The specific structural principle described above, will not repeat them here.

[0113] 综上所述,本发明实施例提供的取样装置及具有该取样装置的生物反应装置,首先由于采用无菌空气保压,并借助上述取样管路上各执行器件来保证无菌状态的前提下,能取出活体生物进行分析与镜检,同时避免了生物形态所受高温干扰的情况。 [0113] In summary, the sampling device according to the present invention and provided with a biological reaction apparatus of the sampling device, first due to the use of sterile air packing, and with the way the executive said sampling tube device to ensure sterility Under the premise of living organisms can be taken out for analysis and microscopic examination, while avoiding the situation biomorphic suffered high temperature interference.

[0114] 其次,由于管道中采用无菌空气进行保压,即可通过蠕动泵的反转,用无菌空气将插入被取样液体中胶管内的残留液体反吹回被取样液体中,既避免了残留液体对实时取样分析结果的干扰,也使通常所需的残留废液排出量降为零;同时依靠蠕动泵正转的时间与流量的预先设定,使每次取样量能实现基于蠕动泵精度的定量化。 [0114] Second, because the pipeline packing sterile air can be reversed by a peristaltic pump, with sterile air will be inserted into the sampling residual liquid inside the cleaning liquid in the hose back to the liquid to be sampled, both to avoid the interference of the real-time sampling residual liquid analysis results, also the residual waste discharge amount normally required zero; while relying on forward peristaltic pump and flow pre-set time, so that the amount of energy each sampling-based motility Pump accuracy quantified.

[0115] 最后,由于通过控制器的程序设定的取样操作步骤,能够使取样操作配合外接自动分析仪进行自动取样并在线分析,也能利用手动取样模式进行临时取样离线分析。 [0115] Finally, since the program is set by the controller sampling procedure, the sampling operation can be automated analyzer with an external automatic sampling and on-line analysis, utilizing manual sampling mode can be temporarily offline sample analysis.

[0116] 本发明提供的取样装置,满足了无菌、取样量的控制与定量、多次取样的差异控制等方面的要求。 Sampling device provided by the invention [0116] The present meets the sterile sample volume control and quantitative differences between multiple sampling control and other requirements.

[0117] 虽然本发明已以较佳实施例揭露如上,然其并非用以限定本发明,任何所属技术领域中具有通常知识者,在不脱离本发明的精神和范围内,当可作各种的更动与润饰,因此本发明的保护范围当视权利要求书所界定者为准。 [0117] While the invention has been revealed as the preferred embodiment, however it is not intended to limit the invention in any technical field have generally intellectuals, without departing from the spirit and scope of the present invention, it is intended that modifications and variations, and therefore the scope of the present invention, when the book depends on the claims and their equivalents.

Patent Citations
Cited PatentFiling datePublication dateApplicantTitle
CN2862007Y *28 Jun 200524 Jan 2007江苏大学New type fermentation automatic sampling valve
CN101971001A *9 Jan 20099 Feb 2011赛托系统有限公司Apparatus and method for filtering biological material
CN102575215A *21 May 201011 Jul 2012Vtt科技研究中心Sample port, multi-layer filter, sampling method, and use of a sample port in sampling
CN203965191U *3 Dec 201326 Nov 2014华东理工大学Sampling device and biological reaction device with same
US4695551 *21 Apr 198622 Sep 1987Proton AgSampling apparatus for a biological reactor
US4942770 *24 Jul 198924 Jul 1990Seifert Gunilla K EAutomatic aseptic sampling apparatus
Non-Patent Citations
Reference
1 *王永红 等: "生物反应器及其研究技术进展", 《生物加工过程》
Classifications
International ClassificationG01N1/10
Legal Events
DateCodeEventDescription
3 Jun 2015C06Publication
1 Jul 2015C10Entry into substantive examination
4 Aug 2017CB03