CN103932703A - Method for recording sleep electrophysiological signals of primates - Google Patents
Method for recording sleep electrophysiological signals of primates Download PDFInfo
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- CN103932703A CN103932703A CN201410195895.9A CN201410195895A CN103932703A CN 103932703 A CN103932703 A CN 103932703A CN 201410195895 A CN201410195895 A CN 201410195895A CN 103932703 A CN103932703 A CN 103932703A
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Abstract
The invention relates to a method for systematically recording sleep electrophysiological signals of primates. The method comprises a first step of simultaneously picking up and recording four-way electrophysiological signals of tested animals; a second step of enabling a four-way bioelectricity signal amplifier to be of a traditional structure of an instrument amplifier and a main amplifier; a third step of connecting the digital output end of a multichannel ADC to an embedded processor and connecting the embedded processor to a TF card and a small liquid crystal display; a fourth step of connecting an I2C host interface of the embedded processor to an I2C slave interface of a real-time clock circuit, connecting an awakening output pin of the clock circuit to an external interruption pin 1 of the embedded processor, and connecting a standby power supply pin of the real-time clock circuit to the positive pole of a silver oxide battery; a fifth step of connecting the output end of a magnetic induction MEMS device to an external interruption pin 2 of the embedded processor; and a sixth step of directly connecting a keyboard to a universal I/O port of the embedded processor.
Description
Technical field
The present invention relates to a kind of can system log (SYSLOG) primate the method for sleep electricity physiological signal.
Background technology
To the research of sleep, have very important significance for many unsolved mysteries of untiing animals and humans brain.Recording the EEG signals under sleep state, and the electricity physiological signals such as EEG signals and the myoelectricity in corresponding moment, eye electricity are comprehensively analyzed, is the important means of the sleep state switching of research humans and animals and Changing Pattern thereof.Owing to can not carry out abrasive experiment, test in live body human brain, the sleep electricity Physiological Experiment of carrying out on the primate approximate with the mankind (as macaque or baboon) just seems particularly important.
Existing brain electricity, myoelectricity or eye electrographic device have the features such as volume is large, power consumption is many conventionally, and animal can not be on one's own initiative from coordinating carrying out of experiment.Therefore, adopt above-mentioned existing equipment to record the electricity physiological signal of animal, just must limit wholly or in part the activity of animal, for example, macaque is placed in to monkey chair fixing.Existing research shows, physical limitation impact will cause the sleep behavior of baboon that the variation of " non-nature " occurs, for example: the pattern of " sleep " to " regaining consciousness " state switching is upset, abnormal drowsiness and aprosexia etc.Although there is no similar research (impact of restriction state on its sleep pattern) on macaque, there are some researches show that the restriction of monkey chair has a significant effect to the normal behaviour of macaque.
Embedded system has all obtained breakthrough progress at the aspect such as power consumption and volume in recent years.But reasonably selecting various embedded schemes, carry out record for primate sleep electricity physiological signal, is technical barrier urgently to be resolved hurrily.
Summary of the invention
Object of the present invention is intended to overcome the deficiencies in the prior art, and a kind of recording method that realizes primate electricity physiological signal by embedded technology is provided.
The recording method of primate sleep electricity physiological signal of the present invention is made up of following steps:
One, pick up simultaneously and record tested animal Si road electricity physiological signal, every road signal amplifier has respectively two difference input electrodes: record end and reference edge, reference ground of the common output of all No. four amplifiers, its electromotive force equals the mid point on supply voltage and ground, for by the common-mode voltage of tested animal bodies, be controlled in the common-mode voltage range that amplifier can normally work; Wherein, for amplifying/pick up the record end of first via amplifier of EEG signals, be directly placed on by surgical operation on the cerebral cortex of frontal lobe portion of tested animal brain; Reference edge is fixed on tested animal parietal bone central area; Be fixed on respectively the both sides of tested Primate biceps brachii m. by surgical operation for amplifying/pick up the record end of No. second amplifier of electromyographic signal and reference edge; The record end and the reference edge that are used for the Third Road amplifier that amplifies/pick up horizontal direction electro-ocular signal are fixed on tested Primate left eye socket of the eye left and right sides skull by surgical operation respectively; Be fixed on respectively the skull of both sides up and down of tested Primate left eye socket of the eye by surgical operation for amplifying/pick up the record end of vertical direction electro-ocular signal No. tetra-amplifiers and reference edge; The reference ground of the common output of all No. four amplifiers is fixed on brow ridge center, tested animal left and right;
Two, four road amplifier of creatures' physical electric signals devices adopt the structure of traditional instrumentation amplifier+main amplifier, wherein, instrumentation amplifier is by the faint electrical biological signals of the difference input of electrode input, be converted to respect to the single-ended voltage signal with reference to ground, with respect to the mid point that equals supply voltage and ground with reference to the electromotive force on ground; Main amplifier is made up of General operational amplifier, after being connected to instrumentation amplifier, more than being responsible for that the single-ended signal with respect to reference to ground is amplified to 60dB; No. four amplifiers export respectively with respect to reference to ground, bioelectrical signals through amplifying, and be connected to the analog input end of four-way ADC, be converted to digital signal;
Three, the interface 1 of the digital output end of multichannel ADC and the SPI of flush bonding processor is connected, and the SPI interface 2 of flush bonding processor is connected to TF card, and the SPI interface 3 of flush bonding processor is connected to small-scale liquid crystal display;
Four, the I2C HPI of flush bonding processor is connected to the I2C slave interface of real time clock circuit; The external interrupt pin 1 that wakes output pin up and be connected to flush bonding processor of real time clock circuit; The stand-by power supply pin of real time clock circuit is connected to the positive pole of silver oxide cell simultaneously;
Five, the output of magnetic induction MEMS device is connected to the external interrupt pin 2 of flush bonding processor;
Six, keyboard is directly connected on the universaling I/O port of flush bonding processor.
Above-mentioned signal amplifier, flush bonding processor, TF card, real time clock circuit, magnetic induction and keyboard all can be taken from prior art.
The structure of instrumentation amplifier+main amplifier that described amplifier of creatures' physical electric signals device adopts can be referring to Analog Device Corporation, " Low Cost; Low Power Instrumentation Amplifier AD620 Data Sheet (Rev. D) " [DB/OL], www.analog.com, 2001. and Analog Device Corporation, " JFET Input Instrumentation Amplifier with Rail-to-Rail Output in MSOP Package (Rev. O) " [DB/OL], www.analog.com, 2006.
Four tunnel low-power consumption amplifier of creatures' physical electric signals devices are respectively used to amplify/pick up brain electricity, eye electricity (horizontal and vertical Ge Yi road) and the electromyographic signal of tested Primate.Thereafter by analog/digital converter (Analog-to-Digital Converter, ADC), Jiang Si road is converted to through the analogue signal of amplifying the digital signal that can store.ADC selects to have the model of more than four difference analogue input channel, as MCP3308.
Flush bonding processor can adopt low-power consumption reduced instruction set computer (RISC) flush bonding processor, feature is low in energy consumption under same treatment speed, its SPI(Serial Peripheral Interface, Serial Peripheral Interface (SPI)) interface 1 is connected to the digital output end of multichannel ADC, to obtain the multichannel bioelectrical signals after digitized.The SPI interface 2 of flush bonding processor is connected to TF card, the various bioelectrical signals that pick up in order to deposit amplifier in mass storage, use TF card (Trans-flash Card) as signal storage medium, and use therein file system (File System) software, the capacity that is characterized in is large, volume is little, low in energy consumption, and storage mass data is more reliable.The SPI interface 3 of flush bonding processor is connected to small-scale liquid crystal display, in order to realize human-computer interaction function.
The I2C HPI of flush bonding processor is connected to the I2C slave interface of real time clock circuit, and realizes the configuration of time and read with this; The external interrupt pin 1 that wakes output pin up and be connected to flush bonding processor of real time clock circuit, in order to the time wake up process device suitable, makes it enter recording status.Employing has real time clock circuit (the Real Time Clock by interrupting arousal function, as IC L1208, DS1337 etc.), being characterized in can be with the low-down power consumption retention time, and wakes flush bonding processor up in the time that needs system log (SYSLOG).Can make processor enter resting state in the time that is not needing record, and at when work wake up process device.The stand-by power supply pin of real time clock circuit is connected to the positive pole of silver oxide cell simultaneously, to ensure that at any time system has correct temporal information.
The output of magnetic induction MEMS device is connected to the external interrupt pin 2 of flush bonding processor, in order to realize the non-contact switch to monitor by Magnet when needed.Adopt magnetic induction MEMS device (Micro-Electro-Mechanical Systems, mems device, as A3212 etc.), MEMS device can be under very little volume and power consumption condition the minor variations of perception external magnetic field.In order to realize the Untouched control to monitor.
By method of the present invention, can not limit primate freedom of action and not affect under the condition of primate ortho sleep, the electricity physiological signal signals such as brain electricity, myoelectricity, eye electricity and electrocardio under continuous its sleep state of non-volatile recording.The recording equipment volume that this method adopts is little, lightweight, can be fixed on the head of primate, convenient and easy.
Brief description of the drawings
Fig. 1 is theory diagram of the present invention.
Fig. 2 is a specific embodiment block diagram of the present invention.
Detailed description of the invention
Concrete embodiment is provided below, and the present invention is described further, but it is not used in restriction the present invention.
Fig. 2 is the physical circuit theory diagram of the thinking design according to the present invention.Its core has adopted has ARM7TDMI-S kernel, by the risc processor LPC2132 of grace intelligence Pu semiconductor production.LPC2132 is 32 arm processors of a new generation that power consumption is extremely low, there is the ability of complexity of processing, can meet that the present invention's design proposes embedded system including the various real-time processing file system, Man Machine Interface and multi-channel data acquisition.In addition, also integrated various peripheral hardwares required for the present invention on LPC2132 sheet, comprise for the real-time clock of timing mode, for controlling SPI interface and the sufficient on-chip memory (RAM and FLASH ROM) of TF storage card and LCD.
LPC2132 has power managed function in addition: under " dormancy " state, LPC2132 enters the king oiscillator halted state that power consumption is very low, and now LPC2132 kernel, owing to losing clock, only has internal storage state to remain unchanged, and operating current is reduced to below 10uA.Due to the operating current of real-time clock integrated in LPC2132 very little (conventionally in 10uA left and right), and this real-time clock can work during in resting state independently at LPC2132, so LPC2132 can realize above-mentioned " timing working " pattern.On daytime, tested animal is in the waking state without record, and now LPC2132 enters " resting state " of very power saving, only has real-time clock to keep timely function in system; In evening, in the time reaching the cut-in time of timing mode setting, in running order real-time clock will be by interrupting always, and the LPC2132 waking up in " resting state " makes it enter " duty " of record data.In Fig. 2, in the lower left corner, " for the silver oxide cell that records of retention time " in the time that system main power source (poly-lithium battery) does not connect, is the real time clock circuit power supply in LPC2132, to prevent resetting system time after each start.
Aspect software, in order to facilitate device and the PC systems exchange data that commercial operation system (Windows, Linux etc.) are housed, in recording equipment, embedded system has been used " file system " software kit ZLG FS increasing income in addition.This file system can be in the system of very little code size, the compatibility of realization and PC file system.
In Fig. 2, the MEMS device A3212 in the lower right corner, is micro-power consumption magnetic field gauge tap device of being produced by Allegro company.When A3212 will have artificial magnetic field to occur in outside, by the control signal that under interrupt notification LPC2132 response magnetic control pattern, experimentation personnel send.
In order to ensure portability and the sufficiently long working time of system, in device, the design of battery power supply system is very crucial.Poly-lithium battery is a kind of to select preferably, and it can be under same volume and weight condition, provide higher than common disposable alkaline cell one times above to capacity.In addition, poly-lithium battery can repeated charge more than 100 times, and major part is all passed through water-proofing treatment.To sum up the system shown in Fig. 2 has been used poly-lithium battery that capacity is 4500mAH as main battery.
For the concrete research of basis requirement, the parameter such as mode of operation, current time of flexible configuration recording equipment, recording equipment leaves Man Machine Interface.In order to improve the portability of device, reduce the discomfort that tested animal is worn, it is only the single color LCD MzL-05 of 1.4 inches that device has adopted diagonal line length.Only 1.5mA of the operating current of MzL-05, in reduction means volume, has effectively extended the working time of same volume and weight battery.
In addition, the recording equipment shown in Fig. 2 can record four road bioelectrical signals simultaneously, and four groups of signal pickup circuit are respectively for brain electricity, myoelectricity, eye electricity and electrocardiosignal.AFE (analog front end) adopted the analog amplify circuit AD623 of low-power consumption and MCP6002 to form signal pickup circuit, and analog to digital conversion circuit has been selected 13 ADC MCP3308 of four-way, fully differential.
Primary processor LPC2132 under duty in signal recording apparatus is operated in 10MHz dominant frequency, together with the IF card of the 2GB capacity of record data, and the small-sized data liquid crystal display MzL-05 of 1.4 inch, its total power consumption is about 20mA 3.3V; Under resting state, in recording equipment, all equipment all enters the exquisite state that power consumption is extremely low, and its total power consumption is about 20uA@3.3V.In the every day of the lower record sleep electricity physiological signal of 8 hours in working order, if selecting capacity is the polymerization road lithium battery of 4500mAH, recording equipment can be after once charging, the continuous sleep electricity physiological signal that records tested animal for 22 days.
Pick up simultaneously and record tested animal Si road electricity physiological signal, every road signal amplifier has respectively two difference input electrodes: record end and reference edge, reference ground of the common output of all No. four amplifiers; Wherein, for amplifying/pick up the record end of first via amplifier of EEG signals, be directly placed on by surgical operation on the cerebral cortex of frontal lobe portion of tested animal brain; It is CZ(Central Zone that reference edge is fixed on tested animal parietal bone central area); Be individually fixed in the both sides of tested Primate biceps brachii m. for amplifying/pick up the record end of No. second amplifier of electromyographic signal and reference edge; Be used for the left and right sides that the record end of the Third Road amplifier that amplifies/pick up level (X) direction electro-ocular signal and reference edge are separately fixed at tested Primate left eye socket of the eye; Be used for amplifying/pick up the both sides up and down that the record end of vertical (Y) direction electro-ocular signal No. tetra-amplifiers and reference edge are separately fixed at tested Primate left eye socket of the eye; The reference ground of the common output of all No. four amplifiers is fixed on brow ridge center, tested animal left and right.
Finally, the package unit including battery, can pack a volume into is 77 × 52 × 35mm(L × W × H) Water-proof aluminium alloy shell in, its gross weight can be controlled at below 250g.This device can be fixed on the head of tested Primate.
Claims (1)
1. a recording method for primate sleep electricity physiological signal, is characterized in that being made up of following steps:
One, pick up simultaneously and record tested animal Si road electricity physiological signal, every road signal amplifier has respectively two difference input electrodes: record end and reference edge, reference ground of the common output of all No. four amplifiers, its electromotive force equals the mid point on supply voltage and ground, for by the common-mode voltage of tested animal bodies, be controlled in the common-mode voltage range that amplifier can normally work; Wherein, for amplifying/pick up the record end of first via amplifier of EEG signals, be directly placed on by surgical operation on the cerebral cortex of frontal lobe portion of tested animal brain; Reference edge is fixed on tested animal parietal bone central area; Be fixed on respectively the both sides of tested Primate biceps brachii m. by surgical operation for amplifying/pick up the record end of No. second amplifier of electromyographic signal and reference edge; The record end and the reference edge that are used for the Third Road amplifier that amplifies/pick up horizontal direction electro-ocular signal are fixed on tested Primate left eye socket of the eye left and right sides skull by surgical operation respectively; Be fixed on respectively the skull of both sides up and down of tested Primate left eye socket of the eye by surgical operation for amplifying/pick up the record end of vertical direction electro-ocular signal No. tetra-amplifiers and reference edge; The reference ground of the common output of all No. four amplifiers is fixed on brow ridge center, tested animal left and right;
Two, four road amplifier of creatures' physical electric signals devices adopt the structure of traditional instrumentation amplifier+main amplifier, wherein, instrumentation amplifier is by the faint electrical biological signals of the difference input of electrode input, be converted to respect to the single-ended voltage signal with reference to ground, with respect to the mid point that equals supply voltage and ground with reference to the electromotive force on ground; Main amplifier is made up of General operational amplifier, after being connected to instrumentation amplifier, more than being responsible for that the single-ended signal with respect to reference to ground is amplified to 60dB; No. four amplifiers export respectively with respect to reference to ground, bioelectrical signals through amplifying, and be connected to the analog input end of four-way ADC, be converted to digital signal;
Three, the interface 1 of the digital output end of multichannel ADC and the SPI of flush bonding processor is connected, and the SPI interface 2 of flush bonding processor is connected to TF card, and the SPI interface 3 of flush bonding processor is connected to small-scale liquid crystal display;
Four, the I2C HPI of flush bonding processor is connected to the I2C slave interface of real time clock circuit; The external interrupt pin 1 that wakes output pin up and be connected to flush bonding processor of real time clock circuit; The stand-by power supply pin of real time clock circuit is connected to the positive pole of silver oxide cell simultaneously;
Five, the output of magnetic induction MEMS device is connected to the external interrupt pin 2 of flush bonding processor;
Six, keyboard is directly connected on the universaling I/O port of flush bonding processor.
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| CN201410195895.9A CN103932703B (en) | 2014-05-09 | 2014-05-09 | The recording method of primate sleep electricity physiological signal and device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105028232A (en) * | 2015-08-12 | 2015-11-11 | 浙江大学 | Automatic grabbing training and recording device for non-human primates |
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2014
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