CN103819231B - A kind of method utilizing municipal sludge to prepare raw vegetables matrix in summer - Google Patents
A kind of method utilizing municipal sludge to prepare raw vegetables matrix in summer Download PDFInfo
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- CN103819231B CN103819231B CN201410072387.1A CN201410072387A CN103819231B CN 103819231 B CN103819231 B CN 103819231B CN 201410072387 A CN201410072387 A CN 201410072387A CN 103819231 B CN103819231 B CN 103819231B
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- 239000011159 matrix material Substances 0.000 title claims abstract description 106
- 239000010802 sludge Substances 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 27
- 235000013311 vegetables Nutrition 0.000 title claims abstract description 26
- 244000068988 Glycine max Species 0.000 claims abstract description 40
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 40
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 36
- 239000007858 starting material Substances 0.000 claims abstract description 36
- 238000000605 extraction Methods 0.000 claims abstract description 35
- 241000894006 Bacteria Species 0.000 claims abstract description 19
- 241000675108 Citrus tangerina Species 0.000 claims abstract description 16
- 238000000855 fermentation Methods 0.000 claims abstract description 14
- 230000004151 fermentation Effects 0.000 claims abstract description 14
- 238000002525 ultrasonication Methods 0.000 claims abstract description 8
- 230000000813 microbial effect Effects 0.000 claims description 31
- 238000003756 stirring Methods 0.000 claims description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 244000057717 Streptococcus lactis Species 0.000 claims description 17
- 235000014897 Streptococcus lactis Nutrition 0.000 claims description 17
- 241000186361 Actinobacteria <class> Species 0.000 claims description 16
- 241000589152 Azotobacter chroococcum Species 0.000 claims description 16
- 241000193755 Bacillus cereus Species 0.000 claims description 16
- 241000589565 Flavobacterium Species 0.000 claims description 16
- 235000007164 Oryza sativa Nutrition 0.000 claims description 16
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 16
- 239000000084 colloidal system Substances 0.000 claims description 16
- 235000009566 rice Nutrition 0.000 claims description 16
- 108010059892 Cellulase Proteins 0.000 claims description 15
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 claims description 15
- 102000035195 Peptidases Human genes 0.000 claims description 15
- 108091005804 Peptidases Proteins 0.000 claims description 15
- 108010043943 Starch Phosphorylase Proteins 0.000 claims description 15
- 239000000872 buffer Substances 0.000 claims description 15
- 229940106157 cellulase Drugs 0.000 claims description 15
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 claims description 15
- 239000002953 phosphate buffered saline Substances 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 12
- 238000002360 preparation method Methods 0.000 claims description 11
- 239000002994 raw material Substances 0.000 claims description 11
- AXTGDCSMTYGJND-UHFFFAOYSA-N 1-dodecylazepan-2-one Chemical compound CCCCCCCCCCCCN1CCCCCC1=O AXTGDCSMTYGJND-UHFFFAOYSA-N 0.000 claims description 10
- 240000005001 Paeonia suffruticosa Species 0.000 claims description 10
- 235000003889 Paeonia suffruticosa Nutrition 0.000 claims description 10
- 240000008042 Zea mays Species 0.000 claims description 10
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 10
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 10
- 238000010438 heat treatment Methods 0.000 claims description 10
- 229960003639 laurocapram Drugs 0.000 claims description 10
- 235000009973 maize Nutrition 0.000 claims description 10
- 239000011259 mixed solution Substances 0.000 claims description 10
- 238000009288 screen filtration Methods 0.000 claims description 10
- 239000010902 straw Substances 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 5
- 238000001556 precipitation Methods 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 235000019833 protease Nutrition 0.000 claims description 4
- 240000007594 Oryza sativa Species 0.000 claims 3
- 230000012010 growth Effects 0.000 abstract description 6
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 239000002699 waste material Substances 0.000 abstract description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 3
- 244000052616 bacterial pathogen Species 0.000 abstract description 2
- 201000010099 disease Diseases 0.000 abstract description 2
- 239000000758 substrate Substances 0.000 abstract description 2
- 230000002110 toxicologic effect Effects 0.000 abstract description 2
- 231100000027 toxicology Toxicity 0.000 abstract description 2
- 241000209094 Oryza Species 0.000 description 13
- 241000196324 Embryophyta Species 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- 235000019219 chocolate Nutrition 0.000 description 2
- 239000002361 compost Substances 0.000 description 2
- 238000009264 composting Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000002417 nutraceutical Substances 0.000 description 2
- 235000021436 nutraceutical agent Nutrition 0.000 description 2
- 235000021049 nutrient content Nutrition 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000448767 Flavobacterium oryzae Species 0.000 description 1
- 241000881860 Paenibacillus mucilaginosus Species 0.000 description 1
- 241000223261 Trichoderma viride Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000007633 bacillus mucilaginosus Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000010841 municipal wastewater Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Abstract
The invention belongs to biological technical field, it is specifically related to a kind of method utilizing municipal sludge to prepare raw vegetables matrix in summer. The present invention is directed to summer vegetable growth harmful bacteria many, the problem of easy disease, is heated to 60-65 DEG C by municipal sludge, effectively eliminate in mud some germs contained, matrix after completing to fermentation more afterwards adds auxiliary, it is possible to suppress harmful bacteria, promote the growth of raw vegetables in summer. And the auxiliary environmentally safe of the present invention, toxicological harmless. The present invention, by the oranges and tangerines adding acetic acid in municipal sludge and rot, afterwards again by ultrasonication, effectively eliminates the heavy metal in municipal sludge, for the safe edible of vegetables provides safeguard. And the present invention uses the oranges and tangerines after rotting, serve the effect turned waste into wealth, while removal heavy metal, solve the problem of the rotten after stain environment of oranges and tangerines. The starter being added with soybean extraction ferments, it is to increase fermented substrate absorbing starter.
Description
Technical field
The invention belongs to biological technical field, it is specifically related to a kind of method utilizing municipal sludge to prepare raw vegetables matrix in summer.
Background technology
Dewatered sludge of urban sewage plant is the solid-state castoff that municipal wastewater treatment plant process waste water produces. Along with China is in the increase building Sewage Plant number, sludge yield still can increase. Containing the plant desired nutritional element such as a large amount of organic matter and N, P and K in municipal sludge, being used as vegetables matrix after compost treatment is realize this type of solid waste reducing, the innoxious effective way with resource utilization.
But containing heavy metal in municipal sludge, at vegetables easy infection disease and pest in summer. And usually adopt the aerobic static compost method of forced ventilation to realize, but wherein one time fermentation needs 30 day time substantially, there is fermentation time length, fermentation not shortcoming thoroughly in this type of waste for process enormous amount, therefore for this problem exploration economy, effective quick composting treatment process, for after the quick composting of city dehydrated sludge as summer vegetables growth matrix to provide theory and technology to support extremely urgent.
Summary of the invention
It is an object of the invention to solve blank of the prior art, it is provided that a kind of method utilizing municipal sludge to prepare raw vegetables matrix in summer, not only efficiently solves the problem of municipal sludge, and it is that the growth of vegetables in summer provides a kind of nutraceutical matrix efficiently.
The technical solution adopted in the present invention is: a kind of method utilizing municipal sludge to prepare raw vegetables matrix in summer, comprises the following steps:
Step one, in municipal sludge, add acetic acid and rotten oranges and tangerines that volumetric molar concentration is 0.7mol/L, stirring after evenly by frequency is the ultrasonication 25min of 20kHz, obtained matrix I after air-dry, the quality of the acetic acid added in often liter of municipal sludge and rotten oranges and tangerines is respectively 32g, 34g;
Step 2, matrix I is put into baking oven heating, until the temperature of matrix I is 60-65 DEG C;
Step 3, the matrix I after maize straw and heating is pulverized, and it is the screen filtration of 1-2mm with aperture respectively, the maize straw after filtering and matrix I mixs according to the mass ratio of 4:1, stirs after evenly and obtain matrix II, for subsequent use;
Step 4, adding water in matrix II, the mass percent concentration making water in matrix II is 68-72%, backward matrix II in add microbial starter culture, and the ratio of matrix II and microbial starter culture is 5:1, and wherein the unit of mixed-matrix I is m3, the unit of microbial starter culture is kg, stirs obtained matrix III after evenly, matrix III is put into the environment that temperature is 30 DEG C, for subsequent use;
Described microbial starter culture is by soybean extraction, subtilis, silicate bacteria, viride, Lactococcus lactis, actinomycetes, colloid bacillus cereus, rice Flavobacterium, yeast, azotobacter chroococcum, cellulase, proteolytic enzyme and Starch phosphorylase are made, and prepare each parts by weight of raw materials number needed for microbial starter culture and be respectively soybean extraction 20-25 part, subtilis 1-2 part, silicate bacteria 3-4 part, viride 2-4 part, Lactococcus lactis 8-12 part, actinomycetes 2-4 part, colloid bacillus cereus 1-2 part, rice Flavobacterium 2 parts, yeast 3-6 part, azotobacter chroococcum 10-13 part, cellulase 8-12 part, proteinase 8-12 parts and Starch phosphorylase 5-6 part,
Step 5, matrix III being proceeded in proving room and to take out after 25 days, obtained matrix IV, in 1-10 days proving rooms of fermentation, strength ofdraft is 40L/min m3, in 16-25 days proving rooms, strength ofdraft is 20L/min m3;
Step 6, matrix IV is pulverized and add auxiliary, namely raw vegetables matrix in summer is obtained, described auxiliary is made up of hundred steps, Fructus Cnidii, tree peony root, Cortex cinnamomi camphorae, laurocapram and water, and the weight part number preparing each raw material needed for auxiliary is respectively hundred step 10-20 parts, Fructus Cnidii 10-12 part, tree peony root 30-40 part, Cortex cinnamomi camphorae 15-18 part, laurocapram 3-4 part, water 900-1000 part.
The preparation method of microbial starter culture described in step 4 comprises the following steps:
The preparation of step one, soybean extraction
(1), soybean is cleaned after dry, pulverize, be the screen filtration of 1mm with diameter afterwards, obtain soyflour, for subsequent use;
(2), by soyflour adding in the phosphate buffered saline buffer that pH is 5.2, volumetric molar concentration is 0.05mol/L, stir obtained mixed solution I after evenly, the phosphate buffered saline buffer volume that every gram of soyflour is corresponding is 10mL;
(3), the mixed solution I that step (2) is obtained is put into the centrifugal 30min of whizzer that rotating speed is 6000r/min, afterwards precipitation is dissolved in the phosphate buffered saline buffer that pH is 6.5, volumetric molar concentration is 0.05mol/L, then centrifugal 15min in the whizzer that rotating speed is 4000r/min is proceeded to, collect supernatant liquor, obtained soybean extraction, for subsequent use;
Step 2, take each component according to the weight part number of described each component;
Step 3, subtilis, silicate bacteria, viride, Lactococcus lactis, actinomycetes, colloid bacillus cereus, rice Flavobacterium, yeast, azotobacter chroococcum are stirred after evenly, progressively add proteolytic enzyme, Starch phosphorylase and cellulase, obtained complex liquid;
Step 4, soybean extraction put into the ultrasonic wave that frequency is 20kHz process 20min, and often process 3min and stop 15s, backward soybean extraction in add the obtained complex liquid of step 3, stir evenly.
Subtilis in the present invention (Bacillussubtilis) culture presevation number is CICC20632; Viride (Trichodermaviride) culture presevation number is CICC40202; Lactococcus lactis (Lactococcuslactis) culture presevation number is CICC23610; Actinomycetes (Dactylosporangiumsp.) culture presevation number is ACCC40661; Azotobacter chroococcum (Azotobacterchrooccum) culture presevation number is ACCC10098; Colloid bacillus cereus (Bacillusmucilaginosus) culture presevation number is ACCC02983; Rice Flavobacterium (Flavobacteriumoryzae) culture presevation number is CGMCC1.1585; Yeast (Saccharomycessp.) culture presevation number is CCTCCAY91003; Above bacterial classification is all purchased from Bei Jingbei Na Chuanlian biotechnology research institute.
Useful effect
One, the present invention is directed to summer vegetable growth harmful bacteria many, the problem of easy disease, is heated to 60-65 DEG C by municipal sludge, effectively eliminate in mud some germs contained, matrix after completing to fermentation more afterwards adds auxiliary, it is possible to suppress harmful bacteria, promote the growth of raw vegetables in summer. And the auxiliary environmentally safe of the present invention, toxicological harmless.
Two, the present invention by adding acetic acid and rotten oranges and tangerines in municipal sludge, afterwards again by ultrasonication, effectively eliminates the heavy metal in municipal sludge, for the safe edible of vegetables provides safeguard. And the present invention uses the oranges and tangerines after rotting, serve the effect turned waste into wealth, while removal heavy metal, solve the problem of the rotten after stain environment of oranges and tangerines.
Three, by taking, the starter being added with soybean extraction ferments in the present invention, greatly reduce intensification and the fermentation time of wheat stalk and municipal sludge fermentation, improve fermented substrate absorbing starter, enriched the nutritive ingredient after fermentation, also improved working efficiency simultaneously.
Four, the soybean extraction in the present invention first mixes with complex liquid by ultrasonication again, the composition in complex liquid and soybean extraction is made to merge evenly, improve municipal sludge and stalk to the absorption rate of starter so that municipal sludge and stalk fermentation are more thorough. In addition, the present invention, having a rest pause with ultrasonic treatment time, avoids ultrasonic wave to the damage of composition in soybean extraction.
Five, the preparation technology of the present invention is simple, by being combined with multiple microorganism, enzyme, it is possible to decomposite the multiple nutrients material of municipal sludge, achieve the effect turned waste into wealth, while being beneficial to urban beautification, for the growth of plant provides nutraceutical matrix.
Embodiment
Utilize municipal sludge to prepare a method for raw vegetables matrix in summer, comprise the following steps:
Step one, in municipal sludge, add acetic acid and rotten oranges and tangerines that volumetric molar concentration is 0.7mol/L, stirring after evenly by frequency is the ultrasonication 25min of 20kHz, obtained matrix I after air-dry, the quality of the acetic acid added in often liter of municipal sludge and rotten oranges and tangerines is respectively 32g, 34g;
Step 2, matrix I is put into baking oven heating, until the temperature of matrix I is 60-65 DEG C;
Step 3, the matrix I after maize straw and heating is pulverized, and it is the screen filtration of 1-2mm with aperture respectively, the maize straw after filtering and matrix I mixs according to the mass ratio of 4:1, stirs after evenly and obtain matrix II, for subsequent use;
Step 4, adding water in matrix II, the mass percent concentration making water in matrix II is 68-72%, backward matrix II in add microbial starter culture, and the ratio of matrix II and microbial starter culture is 5:1, and wherein the unit of mixed-matrix I is m3, the unit of microbial starter culture is kg, stirs obtained matrix III after evenly, matrix III is put into the environment that temperature is 30 DEG C, for subsequent use;
Described microbial starter culture is by soybean extraction, subtilis, silicate bacteria, viride, Lactococcus lactis, actinomycetes, colloid bacillus cereus, rice Flavobacterium, yeast, azotobacter chroococcum, cellulase, proteolytic enzyme and Starch phosphorylase are made, and prepare each parts by weight of raw materials number needed for microbial starter culture and be respectively soybean extraction 20-25 part, subtilis 1-2 part, silicate bacteria 3-4 part, viride 2-4 part, Lactococcus lactis 8-12 part, actinomycetes 2-4 part, colloid bacillus cereus 1-2 part, rice Flavobacterium 2 parts, yeast 3-6 part, azotobacter chroococcum 10-13 part, cellulase 8-12 part, proteinase 8-12 parts and Starch phosphorylase 5-6 part,
Step 5, matrix III being proceeded in proving room and to take out after 25 days, obtained matrix IV, in 1-10 days proving rooms of fermentation, strength ofdraft is 40L/min m3, in 16-25 days proving rooms, strength ofdraft is 20L/min m3;
Step 6, matrix IV is pulverized and add auxiliary, namely raw vegetables matrix in summer is obtained, described auxiliary is made up of hundred steps, Fructus Cnidii, tree peony root, Cortex cinnamomi camphorae, laurocapram and water, and the weight part number preparing each raw material needed for auxiliary is respectively hundred step 10-20 parts, Fructus Cnidii 10-12 part, tree peony root 30-40 part, Cortex cinnamomi camphorae 15-18 part, laurocapram 3-4 part, water 900-1000 part.
The preparation method of microbial starter culture described in step 4 comprises the following steps:
The preparation of step one, soybean extraction
(1), soybean is cleaned after dry, pulverize, be the screen filtration of 1mm with diameter afterwards, obtain soyflour, for subsequent use;
(2), by soyflour adding in the phosphate buffered saline buffer that pH is 5.2, volumetric molar concentration is 0.05mol/L, stir obtained mixed solution I after evenly, the phosphate buffered saline buffer volume that every gram of soyflour is corresponding is 10mL;
(3), the mixed solution I that step (2) is obtained is put into the centrifugal 30min of whizzer that rotating speed is 6000r/min, afterwards precipitation is dissolved in the phosphate buffered saline buffer that pH is 6.5, volumetric molar concentration is 0.05mol/L, then centrifugal 15min in the whizzer that rotating speed is 4000r/min is proceeded to, collect supernatant liquor, obtained soybean extraction, for subsequent use;
Step 2, take each component according to the weight part number of described each component;
Step 3, subtilis, silicate bacteria, viride, Lactococcus lactis, actinomycetes, colloid bacillus cereus, rice Flavobacterium, yeast, azotobacter chroococcum are stirred after evenly, progressively add proteolytic enzyme, Starch phosphorylase and cellulase, obtained complex liquid;
Step 4, soybean extraction put into the ultrasonic wave that frequency is 20kHz process 20min, and often process 3min and stop 15s, backward soybean extraction in add the obtained complex liquid of step 3, stir evenly.
Embodiment 1
Utilize municipal sludge to prepare a method for raw vegetables matrix in summer, comprise the following steps:
Step one, in municipal sludge, add acetic acid and rotten oranges and tangerines that volumetric molar concentration is 0.7mol/L, stirring after evenly by frequency is the ultrasonication 25min of 20kHz, obtained matrix I after air-dry, the quality of the acetic acid added in often liter of municipal sludge and rotten oranges and tangerines is respectively 32g, 34g;
Step 2, matrix I is put into baking oven heating, until the temperature of matrix I is 60 DEG C;
Step 3, the matrix I after maize straw and heating is pulverized, and it is the screen filtration of 1-2mm with aperture respectively, the maize straw after filtering and matrix I mixs according to the mass ratio of 4:1, stirs after evenly and obtain matrix II, for subsequent use;
Step 4, adding water in matrix II, the mass percent concentration making water in matrix II is 68-72%, backward matrix II in add microbial starter culture, and the ratio of matrix II and microbial starter culture is 5:1, and wherein the unit of mixed-matrix I is m3, the unit of microbial starter culture is kg, stirs obtained matrix III after evenly, matrix III is put into the environment that temperature is 30 DEG C, for subsequent use;
Described microbial starter culture is by soybean extraction, subtilis, silicate bacteria, viride, Lactococcus lactis, actinomycetes, colloid bacillus cereus, rice Flavobacterium, yeast, azotobacter chroococcum, cellulase, proteolytic enzyme and Starch phosphorylase are made, and prepare each parts by weight of raw materials number needed for microbial starter culture and be respectively soybean extraction 20 parts, subtilis 1 part, silicate bacteria 3 parts, viride 2 parts, Lactococcus lactis 8 parts, 2 parts, actinomycetes, colloid bacillus cereus 1 part, rice Flavobacterium 2 parts, 3 parts, yeast, azotobacter chroococcum 10 parts, cellulase 8 parts, proteinase 8 part and Starch phosphorylase 5 parts,
Step 5, matrix III being proceeded in proving room and to take out after 25 days, obtained matrix IV, in 1-10 days proving rooms of fermentation, strength ofdraft is 40L/min m3, in 16-25 days proving rooms, strength ofdraft is 20L/min m3;
Step 6, matrix IV is pulverized and add auxiliary, namely raw vegetables matrix in summer is obtained, described auxiliary is made up of hundred steps, Fructus Cnidii, tree peony root, Cortex cinnamomi camphorae, laurocapram and water, and the weight part number preparing each raw material needed for auxiliary is respectively hundred step 10 parts, Fructus Cnidii 10 parts, tree peony root 30 parts, Cortex cinnamomi camphorae 15 parts, laurocapram 3 parts, 900 parts, water.
The preparation method of microbial starter culture described in step 4 comprises the following steps:
The preparation of step one, soybean extraction
(1), soybean is cleaned after dry, pulverize, be the screen filtration of 1mm with diameter afterwards, obtain soyflour, for subsequent use;
(2), by soyflour adding in the phosphate buffered saline buffer that pH is 5.2, volumetric molar concentration is 0.05mol/L, stir obtained mixed solution I after evenly, the phosphate buffered saline buffer volume that every gram of soyflour is corresponding is 10mL;
(3), the mixed solution I that step (2) is obtained is put into the centrifugal 30min of whizzer that rotating speed is 6000r/min, afterwards precipitation is dissolved in the phosphate buffered saline buffer that pH is 6.5, volumetric molar concentration is 0.05mol/L, then centrifugal 15min in the whizzer that rotating speed is 4000r/min is proceeded to, collect supernatant liquor, obtained soybean extraction, for subsequent use;
Step 2, take each component according to the weight part number of described each component;
Step 3, subtilis, silicate bacteria, viride, Lactococcus lactis, actinomycetes, colloid bacillus cereus, rice Flavobacterium, yeast, azotobacter chroococcum are stirred after evenly, progressively add proteolytic enzyme, Starch phosphorylase and cellulase, obtained complex liquid;
Step 4, soybean extraction put into the ultrasonic wave that frequency is 20kHz process 20min, and often process 3min and stop 15s, backward soybean extraction in add the obtained complex liquid of step 3, stir evenly.
Result is tested: the matrix color after utilizing aforesaid method to become thoroughly decomposed is chocolate, moisture content 44%, and the C/N ratio of matrix is reduced to 12.3. 60 DEG C of hot stages can maintain 7 days, and T=(C/N is eventually)/(C/N beginning) T < 0.6. Quick-acting N-P-K content is respectively 0.66mg/kg, 835.25mg/kg, 3591.1mg/kg. This available nutrient content can meet the needs of plant-growth completely.
Embodiment 2
Utilize municipal sludge to prepare a method for raw vegetables matrix in summer, comprise the following steps:
Step one, in municipal sludge, add acetic acid and rotten oranges and tangerines that volumetric molar concentration is 0.7mol/L, stirring after evenly by frequency is the ultrasonication 25min of 20kHz, obtained matrix I after air-dry, the quality of the acetic acid added in often liter of municipal sludge and rotten oranges and tangerines is respectively 32g, 34g;
Step 2, matrix I is put into baking oven heating, until the temperature of matrix I is 65 DEG C;
Step 3, the matrix I after maize straw and heating is pulverized, and it is the screen filtration of 2mm with aperture respectively, the maize straw after filtering and matrix I mixs according to the mass ratio of 4:1, stirs after evenly and obtain matrix II, for subsequent use;
Step 4, adding water in matrix II, the mass percent concentration making water in matrix II is 68-72%, backward matrix II in add microbial starter culture, and the ratio of matrix II and microbial starter culture is 5:1, and wherein the unit of mixed-matrix I is m3, the unit of microbial starter culture is kg, stirs obtained matrix III after evenly, matrix III is put into the environment that temperature is 30 DEG C, for subsequent use;
Described microbial starter culture is by soybean extraction, subtilis, silicate bacteria, viride, Lactococcus lactis, actinomycetes, colloid bacillus cereus, rice Flavobacterium, yeast, azotobacter chroococcum, cellulase, proteolytic enzyme and Starch phosphorylase are made, and prepare each parts by weight of raw materials number needed for microbial starter culture and be respectively soybean extraction 25 parts, subtilis 2 parts, silicate bacteria 4 parts, viride 4 parts, Lactococcus lactis 12 parts, 4 parts, actinomycetes, colloid bacillus cereus 2 parts, rice Flavobacterium 2 parts, 6 parts, yeast, azotobacter chroococcum 13 parts, cellulase 12 parts, 12 parts, proteolytic enzyme and Starch phosphorylase 6 parts,
Step 5, matrix III being proceeded in proving room and to take out after 25 days, obtained matrix IV, in 1-10 days proving rooms of fermentation, strength ofdraft is 40L/min m3, in 16-25 days proving rooms, strength ofdraft is 20L/min m3;
Step 6, matrix IV is pulverized and add auxiliary, namely raw vegetables matrix in summer is obtained, described auxiliary is made up of hundred steps, Fructus Cnidii, tree peony root, Cortex cinnamomi camphorae, laurocapram and water, and the weight part number preparing each raw material needed for auxiliary is respectively hundred step 20 parts, Fructus Cnidii 12 parts, tree peony root 40 parts, Cortex cinnamomi camphorae 18 parts, laurocapram 4 parts, 1000 parts, water.
The preparation method of microbial starter culture described in step 4 comprises the following steps:
The preparation of step one, soybean extraction
(1), soybean is cleaned after dry, pulverize, be the screen filtration of 1mm with diameter afterwards, obtain soyflour, for subsequent use;
(2), by soyflour adding in the phosphate buffered saline buffer that pH is 5.2, volumetric molar concentration is 0.05mol/L, stir obtained mixed solution I after evenly, the phosphate buffered saline buffer volume that every gram of soyflour is corresponding is 10mL;
(3), the mixed solution I that step (2) is obtained is put into the centrifugal 30min of whizzer that rotating speed is 6000r/min, afterwards precipitation is dissolved in the phosphate buffered saline buffer that pH is 6.5, volumetric molar concentration is 0.05mol/L, then centrifugal 15min in the whizzer that rotating speed is 4000r/min is proceeded to, collect supernatant liquor, obtained soybean extraction, for subsequent use;
Step 2, take each component according to the weight part number of described each component;
Step 3, subtilis, silicate bacteria, viride, Lactococcus lactis, actinomycetes, colloid bacillus cereus, rice Flavobacterium, yeast, azotobacter chroococcum are stirred after evenly, progressively add proteolytic enzyme, Starch phosphorylase and cellulase, obtained complex liquid;
Step 4, soybean extraction put into the ultrasonic wave that frequency is 20kHz process 20min, and often process 3min and stop 15s, backward soybean extraction in add the obtained complex liquid of step 3, stir evenly.
Result is tested: the matrix color after utilizing aforesaid method to become thoroughly decomposed is chocolate, moisture content 43%, and the C/N ratio of matrix is reduced to 11.9. 60 DEG C of hot stages can maintain 9 days, and T=(C/N is eventually)/(C/N beginning) T < 0.6. Quick-acting N-P-K content is respectively 0.78mg/kg, 841.25mg/kg, 3594.34mg/kg. This available nutrient content can meet the needs of plant-growth completely.
Claims (2)
1. one kind utilizes the method that municipal sludge prepares raw vegetables matrix in summer, it is characterised in that, comprise the following steps:
Step one, in municipal sludge, add acetic acid and rotten oranges and tangerines that volumetric molar concentration is 0.7mol/L, stirring after evenly by frequency is the ultrasonication 25min of 20kHz, obtained matrix I after air-dry, the quality of the acetic acid added in often liter of municipal sludge and rotten oranges and tangerines is respectively 32g, 34g;
Step 2, matrix I is put into baking oven heating, until the temperature of matrix I is 60-65 DEG C;
Step 3, the matrix I after maize straw and heating is pulverized, and it is the screen filtration of 1-2mm with aperture respectively, the maize straw after filtering and matrix I mixs according to the mass ratio of 4:1, stirs after evenly and obtain matrix II, for subsequent use;
Step 4, adding water in matrix II, the mass percent concentration making water in matrix II is 68-72%, backward matrix II in add microbial starter culture, and the ratio of matrix II and microbial starter culture is 5:1, and wherein the unit of mixed-matrix II is m3, the unit of microbial starter culture is kg, stirs obtained matrix III after evenly, matrix III is put into the environment that temperature is 30 DEG C, for subsequent use;
Described microbial starter culture is by soybean extraction, subtilis, silicate bacteria, viride, Lactococcus lactis, actinomycetes, colloid bacillus cereus, rice Flavobacterium, yeast, azotobacter chroococcum, cellulase, proteolytic enzyme and Starch phosphorylase are made, and prepare each parts by weight of raw materials number needed for microbial starter culture and be respectively soybean extraction 20-25 part, subtilis 1-2 part, silicate bacteria 3-4 part, viride 2-4 part, Lactococcus lactis 8-12 part, actinomycetes 2-4 part, colloid bacillus cereus 1-2 part, rice Flavobacterium 2 parts, yeast 3-6 part, azotobacter chroococcum 10-13 part, cellulase 8-12 part, proteinase 8-12 parts and Starch phosphorylase 5-6 part,
Step 5, matrix III is proceeded in proving room and to take out after 25 days, obtained matrix IV;
Step 6, matrix IV is pulverized and add auxiliary, namely raw vegetables matrix in summer is obtained, described auxiliary is made up of hundred steps, Fructus Cnidii, tree peony root, Cortex cinnamomi camphorae, laurocapram and water, and the weight part number preparing each raw material needed for auxiliary is respectively hundred step 10-20 parts, Fructus Cnidii 10-12 part, tree peony root 30-40 part, Cortex cinnamomi camphorae 15-18 part, laurocapram 3-4 part, water 900-1000 part;
In described step 4, the preparation method of microbial starter culture comprises the following steps:
The preparation of step (), soybean extraction
(1), soybean is cleaned after dry, pulverize, be the screen filtration of 1mm with diameter afterwards, obtain soyflour, for subsequent use;
(2), by soyflour adding in the phosphate buffered saline buffer that pH is 5.2, volumetric molar concentration is 0.05mol/L, stir obtained mixed solution I after evenly, the phosphate buffered saline buffer volume that every gram of soyflour is corresponding is 10mL;
(3), the mixed solution I that step (2) is obtained is put into the centrifugal 30min of whizzer that rotating speed is 6000r/min, afterwards precipitation is dissolved in the phosphate buffered saline buffer that pH is 6.5, volumetric molar concentration is 0.05mol/L, then centrifugal 15min in the whizzer that rotating speed is 4000r/min is proceeded to, collect supernatant liquor, obtained soybean extraction, for subsequent use;
Step (two), prepare each raw material needed for microbial starter culture take each component according to above-mentioned;
Step (three), subtilis, silicate bacteria, viride, Lactococcus lactis, actinomycetes, colloid bacillus cereus, rice Flavobacterium, yeast, azotobacter chroococcum are stirred after evenly, progressively add proteolytic enzyme, Starch phosphorylase and cellulase, obtained complex liquid;
Step (four), soybean extraction put into the ultrasonic wave that frequency is 20kHz process 20min, and often process 3min and stop 15s, backward soybean extraction in add the obtained complex liquid of step (three), stir evenly.
2. a kind of method utilizing municipal sludge to prepare raw vegetables matrix in summer according to claim 1, it is characterised in that: in 1-10 days proving rooms of fermentation, strength ofdraft is 40L/min m3, in 16-25 days proving rooms, strength ofdraft is 20L/min m3��
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4267049A (en) * | 1978-02-13 | 1981-05-12 | Erickson Lennart G | Biological sludge-energy recycling method |
| CN101509016A (en) * | 2009-03-23 | 2009-08-19 | 西南大学 | Method for diphasic fermentation of generating matter energy by using agricultural product castoff such as tangerine hull slag |
| CN102992836A (en) * | 2012-10-10 | 2013-03-27 | 肥西县农业技术推广中心 | Method for preparing medicinal fertilizer special for corns |
| CN103467148A (en) * | 2013-09-07 | 2013-12-25 | 李拓彬 | Organic fertilizer prepared from sludge |
| CN103467149A (en) * | 2013-09-17 | 2013-12-25 | 厦门绿标生物科技有限公司 | Method for processing sludge by using complex microbial agent |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4267049A (en) * | 1978-02-13 | 1981-05-12 | Erickson Lennart G | Biological sludge-energy recycling method |
| CN101509016A (en) * | 2009-03-23 | 2009-08-19 | 西南大学 | Method for diphasic fermentation of generating matter energy by using agricultural product castoff such as tangerine hull slag |
| CN102992836A (en) * | 2012-10-10 | 2013-03-27 | 肥西县农业技术推广中心 | Method for preparing medicinal fertilizer special for corns |
| CN103467148A (en) * | 2013-09-07 | 2013-12-25 | 李拓彬 | Organic fertilizer prepared from sludge |
| CN103467149A (en) * | 2013-09-17 | 2013-12-25 | 厦门绿标生物科技有限公司 | Method for processing sludge by using complex microbial agent |
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