CN103645130B - Visible component High-throughput quantitative analysis method in human or animal's excretion thing - Google Patents
Visible component High-throughput quantitative analysis method in human or animal's excretion thing Download PDFInfo
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- CN103645130B CN103645130B CN201310532007.3A CN201310532007A CN103645130B CN 103645130 B CN103645130 B CN 103645130B CN 201310532007 A CN201310532007 A CN 201310532007A CN 103645130 B CN103645130 B CN 103645130B
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Abstract
The present invention relates to a kind of visible component High-throughput quantitative analysis method in human or animal's excretion thing, it includes that limitation samples, quantitatively dilutes, stirs, measures, injects the step such as counting chamber, image acquisition analysis, the advantage of present invention maximum is can be with quantitative analysis, can definitely measure the visible component quantity in human or animal's excretion thing unit volume or Unit Weight, its result is accurate, can avoid the uncertainty of tradition sampling and analyzing method.
Description
Technical field
The present invention relates to medical inspection field, particularly relate to visible component high-throughput quantification in a kind of human or animal's excretion thing
Analysis method.
Background technology
Collection and check analysis process currently for human or animal's excretion thing are as follows: take a part of excretion thing and be put into appearance
In device, in carton or plastic cup, control laboratory sent into by container by collection personnel, and 2 diluents are dropped on slide by reviewer,
Take the sample of Semen phaseoli radiati size from container with cotton swab or little bamboo chip again to be coated on slide, stir, then put and see under the microscope
Examine.Observe the character of science such as the color of sample, hardness, mucus simultaneously, observe the quantity of worm's ovum with low power lens, as protozoon, hook worm,
The egg counts of ascarid, then observe the visible component in sample with high power lens, such as erythrocyte, leukocyte, mould mycelia or special knot
Brilliant quantity.As the situation of occulting blood need to be checked, then use the sample drop after taking above dilution on reagent paper, observe the change feelings of reagent paper
Condition.Then providing survey report according to the result of above-mentioned observation, the content of survey report includes the character of sample, such as color, hard
Degree, mucus, also include the microscopy result of sample, as containing how many worm's ovums, how many erythrocyte etc., and the test knot occulted blood
Really.Above the collection of human or animal's excretion thing and check analysis process are existed bio-safety hidden danger, operate unhygienic, abnormal smells from the patient is big
Etc. shortcoming, testing result is unstable, it is difficult to the assay that repetition is same, and same sample is carried out multi collect inspection
Data, deviation is very big, and operating process can not standardization.
Summary of the invention
It is an object of the invention to overcome existing methodological deficiency, it is provided that one meets bio-safety standard, operated
Visible component High-throughput quantitative analysis method in journey standardization, testing result human or animal's excretion thing accurately.
The present invention is achieved by the following technical programs: visible component high-throughput quantification in human or animal's excretion thing
Analysis method, it is characterised in that: it comprises the following steps:
(1) human or animal's excretion thing of limitation is taken with sampler, as measuring samples；
(2) take a known volume or the container of weight, quantitative diluent and measuring samples are joined in this container；
(3) diluent is stirred with measuring samples, form suspension；
(4) accurately measure adding diluent volume of a container or weight with measuring samples by measuring instrument；
(5) take one block of detection plate, be provided with the counting chamber of a known depth in the middle part of detection plate, suspension is filled counting
Pond；
(6) counting chamber filling suspension is placed under the microscope of known visual field area and carries out image acquisition analysis.
Further, the sampler in described step (1) is that a lower end sets vaned sampling rod, and described blade has many
Individual, it is bound up in centre, in air vane lamellar, interval forms the area of space of fixed size from each other, will take
Lining bar inserts in human or animal's excretion thing, rotates sampling rod, the region that excretion thing is i.e. adopted between blade.
Further, the stirring in described step (3) is that the mode using magnetic agitation is carried out, and is first added by magnetic bodies
Enter to install in the container of diluent and measuring samples, then in the additional magnetic field being continually changing of container, make magnetic bodies exist
Constantly rotate under variable magnetic force effect, make diluent and measuring samples be sufficiently mixed uniformly, form suspension.
Preferably, the measuring instrument described in described step (4) is CCD scanning sensor, with CCD scanning sensor to appearance
Suspension volume in device is accurately measured.
Preferably, the measuring instrument described in described step (4) is analytical balance, with analytical balance to comprising suspension
Container carries out accurate weighing.
Further, the detection plate in described step (5) is continuous flow detection plate, and described continuous flow detection plate includes
Inlet tube, counting chamber and outlet, described inlet tube and outlet be located at counting chamber two ends, and connect with counting chamber, described meter
The degree of depth in number pond is known numeric value, and suspension enters counting chamber by inlet tube, carries out image acquisition under the microscope.
Preferably, above-mentioned steps (1)～(6) are to carry out in the closed system of normal temperature and pressure.
The positive effect of the present invention is:
(1) compared with existing analysis method, the advantage of present invention maximum is can definitely measure people with quantitative analysis
Or animal excretion thing unit volume is interior or the visible component quantity of Unit Weight, its result degree of accuracy is high, error is little, representative
By force, the uncertainty of tradition sampling and analyzing method, the one-sidedness of analysis result can be avoided, prevent mistaken diagnosis.
(2) the inventive method process is rigorous, has quantitative, it is possible to achieve scientific, the standardization of sample analysis process.
(3) present invention uses sampling rod to sample, and the bottom of sampling rod is the sampling head of air vane chip, and operator only need
Rotating sampling rod, the region that sample is i.e. adopted between middle, the quantity of sampling is relatively stable, operating process is simple, health, section
Learn.
(4) present invention uses magnetic agitation, in the additional magnetic field being continually changing of container, makes magnetic bodies at change magnetic
Constantly rotate under power effect, make diluent and measuring samples be sufficiently mixed uniformly, form suspension.This alr mode and tradition
Stir with spillikin or little rod and compare, there is the remarkable advantages such as mixing speed is fast, mixing effect is good.
(5) present invention sampling, dilution, magnetic agitation, counting chamber are observed, and speed is fast,！Compared with traditional method, have bright
Aobvious high flux advantage.
(6) whole experiment detection process is carried out in closed system, odorlessness, pollution-free, beneficially laboratory biological
Protection, meets bio-safety requirement.
Accompanying drawing explanation
Fig. 1 is the structural representation of the sampling rod in the embodiment of the present invention 1.
Fig. 2 is the structural representation of the continuous flow detection plate in the embodiment of the present invention 1.
Fig. 3 is the structural representation of the fixed detection plate in the embodiment of the present invention 2.
In accompanying drawing: 1 sampling rod, 2 blades, 3 centre bores, 4 inlet tubes, 5 counting chambers, 6 outlets
Pipe, 7 fixed detection plates, 8 detection cells.
Detailed description of the invention
The specific implementation process of the inventive method is described with embodiment below, is explanation of the invention rather than right
The restriction of the present invention, protection scope of the present invention is not limited only to this.Improvement that any principle according to the present invention, method are done,
Variation, within all including protection scope of the present invention.
Embodiment 1
As a example by the excretion thing of following employment, use the metering system of CCD scanning sensor that the inventive method is made further
Explanation.
(1) sampler, as it is shown in figure 1, sampler is the sampling rod 1 that a lower end is provided with blade 2, sampling rod 1 are taken
The blade 2 of lower end has multiple, is bound up in centre, and in air vane lamellar, interval forms fixed size from each other
Area of space；Sampling rod centre position is provided with a centre bore 3, is used for adding diluent, and centre bore 3 sets in the intersection of blade
Having multiple outlet, described outlet is for being uniformly distributed.Being inserted by sampling rod 1 in the excretion thing of people, rotate sampling rod 1, excretion thing is i.e.
The region adopted between blade.Owing to the region between the blade 2 of sampling rod 1 lower end is fixing, through meter when being design
Calculating, so the measuring samples gathered is a finite quantity every time, its numerical value is within the scope of design load.In the present embodiment,
Assume that the measuring samples that sampling rod 1 is gathered is V1.
(2) taking a sectional area is the elliptical vessel of S, is vertically disposed in ellipse by the sampling rod 1 gathering measuring samples
Above bulge, then the diluent that volume is V2 is added in the centre bore 3 of sampling rod 1, by centre bore 3 to sampling rod 1
Measuring samples between blade is rinsed, and makes measuring samples and the diluent that volume is V2 join in elliptical vessel.
(3) diluent is stirred with measuring samples, form suspension.Described alr mode is magnetic agitation, by body
Amass the little bar magnet for V3 to put in elliptical vessel.Again in the additional magnetic field being continually changing of container, make little bar magnet in change
Constantly rotating under magneticaction, mixing time is about 1～5 minute, makes diluent and measuring samples be sufficiently mixed uniformly, is formed mixed
Suspension.This alr mode with traditional stir with spillikin or little rod compared with, there is health, mixing speed is fast, mixing effect good
Deng remarkable advantage.
(4) height amassed as the suspension in the elliptical vessel of S with CCD scanning sensor pair cross-section is accurately swept
Retouch.Assume that the height value that scanning obtains is H, then we just can calculate the volume of suspension is V4=S*H-V3, also the most permissible
Accurately calculate the volume V1=V4-V2=S*H-V3-V2 of sample to be detected in container, thus calculate the concentration of suspension.
(5) taking one block of detection plate, as in figure 2 it is shown, detection plate is continuous flow detection plate, continuous flow detection plate includes
Inlet tube 4, counting chamber 5 and outlet 6, described inlet tube 4 and outlet 6 be located at counting chamber 5 two ends, and connect with counting chamber 5,
The degree of depth of described counting chamber 5 is H2, and suspension fills counting chamber 5 by inlet tube.
(6) counting chamber 5 filling suspension be placed under the microscope that visual field area is S2, carry out image acquisition analysis,
It is known that erythrocyte, leukocyte, worm's ovum, mould mycelia or special crystallization etc. contained by the suspension that volume is V5=S2*H2
The quantity of visible component, then we the most just can calculate the quantity of visible component contained by suspension in unit volume.Root again
According to the concentration of calculated suspension in step (4), it is possible to calculate definitely and do not add measuring samples unit bodies before diluent
The quantity of contained visible component in long-pending, thus provide quantitative testing report accurately.
Above-mentioned steps (1)～(6) are to carry out in the closed system of normal temperature and pressure, odorlessness, pollution-free, are beneficial to experiment
Room biological protection, meets bio-safety requirement.
Embodiment 2
Below in the way of Weighing, the inventive method is further elaborated.
(1) sampler by a limitation gathers the excretion thing of people, as measuring samples.
(2) taking a weight is the container of G1, measuring samples and the diluent that weight is G2 is added in container.
(3) diluent is stirred with measuring samples, form suspension.Described alr mode is common manual stirring,
Stirring rod being put in container and be stirred continuously, mixing time is about 5 minutes, makes diluent and measuring samples be sufficiently mixed uniformly,
Form suspension.
(4) with 100,000/analytical balance, the container equipped with suspension is precisely weighed, it is assumed that the weight of weighing
G3.Just the weight that can thus calculate the sample to be detected in container is G4=G3-G2-G1, thus calculates the dense of suspension
Degree.
(5) one block of detection plate, as it is shown on figure 3, described detection plate is fixed detection plate 7, described fixed detection plate 7 are taken
Middle part is provided with the detection cell 8 that known depth is H2, and suspension fills detection cell 8.
(6) detection cell 8 filling suspension being placed on a known visual field area is the basis of microscopic observation of S2, it is possible to
Know that contained by the suspension that volume is V5=S2*H2, erythrocyte, leukocyte, worm's ovum, mould mycelia or special crystallization etc. have formation
Part quantity, then we the most just can calculate the quantity of visible component contained by suspension in unit volume.Further according to step
(4) concentration of calculated suspension in, it is possible to calculate definitely and do not add before diluent institute in measuring samples Unit Weight
Quantity containing visible component, thus provide quantitative testing report accurately.
Above-mentioned steps (1)～(6) are to carry out in the closed system of normal temperature and pressure, odorlessness, pollution-free, are beneficial to experiment
Room biological protection, meets bio-safety requirement.
Both examples above is explanation of the invention, and non-limiting, is more than PART.It should be noted that
It is, it will be apparent to those skilled in the art that the simple transformation carried out according to ultimate principle and the structure of the inventive method and improvement,
Within being included in protection scope of the present invention.
Claims (1)
1. visible component High-throughput quantitative analysis method in human or animal's excretion thing, it comprises the following steps: take limit with sampler
Human or animal's excretion thing of amount, as measuring samples；Take a known volume or the container of weight, by quantitative diluent and treating
Sample product join in this container；Diluent is stirred with measuring samples, forms suspension；By measuring instrument to
The volume of a container or the weight that add diluent and measuring samples are accurately measured；Take one block of detection plate, set in the middle part of detection plate
There is the counting chamber of a known depth, suspension is filled counting chamber；The counting chamber filling suspension is placed on known sight plane
Image acquisition analysis is carried out under long-pending microscope, it is characterised in that: described sampler is that a lower end sets vaned sampling rod,
Described blade has multiple, is bound up in centre, and in air vane lamellar, interval forms the sky of fixed size from each other
Between region, sampling rod is inserted in human or animal's excretion thing, rotate sampling rod, the region that excretion thing is i.e. adopted between blade.
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| CN201310532007.3A CN103645130B (en) | 2013-11-03 | 2013-11-03 | Visible component High-throughput quantitative analysis method in human or animal's excretion thing |
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| CN201310532007.3A CN103645130B (en) | 2013-11-03 | 2013-11-03 | Visible component High-throughput quantitative analysis method in human or animal's excretion thing |
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| CN103645130B true CN103645130B (en) | 2016-08-17 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104166005B (en) * | 2014-08-20 | 2015-09-02 | 湖南欧杰生物科技发展有限公司 | A kind of full-automatic excretion thing detector |
| CN105092283B (en) * | 2015-06-10 | 2018-09-25 | 广州市康立明生物科技有限责任公司 | A kind of sampling rod for stool sampling device |
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| CN101487845A (en) * | 2009-02-19 | 2009-07-22 | 上海吉鸿生物科技有限公司 | Automatic detection instrument for excrement sample |
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| CN102224260A (en) * | 2008-09-24 | 2011-10-19 | 施特劳斯控股公司 | Kits and devices for detecting analytes |
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| CN203259449U (en) * | 2013-04-26 | 2013-10-30 | 江西金洹生物科技有限公司 | Flow counting chamber with variable thickness |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3263729B2 (en) * | 1999-09-03 | 2002-03-11 | 独立行政法人産業技術総合研究所 | Apparatus and method for measuring particles in liquid |
| US20050273271A1 (en) * | 2004-04-05 | 2005-12-08 | Aibing Rao | Method of characterizing cell shape |
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Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US5356793A (en) * | 1990-03-15 | 1994-10-18 | Nitta Gelatin Inc. | Method for testing the sensitivity of anticancer drug |
| US5848177A (en) * | 1994-12-29 | 1998-12-08 | Board Of Trustees Operating Michigan State University | Method and system for detection of biological materials using fractal dimensions |
| CN102224260A (en) * | 2008-09-24 | 2011-10-19 | 施特劳斯控股公司 | Kits and devices for detecting analytes |
| CN101637668A (en) * | 2009-01-14 | 2010-02-03 | 中山大学 | Device and method for combined use of molecular imprinting solid phase microextraction and hollow fiber liquid phase microextraction, and application thereof |
| CN101487845A (en) * | 2009-02-19 | 2009-07-22 | 上海吉鸿生物科技有限公司 | Automatic detection instrument for excrement sample |
| CN202939053U (en) * | 2012-10-31 | 2013-05-15 | 屈雅川 | Simple multifunctional disposable stool sampler |
| CN203132980U (en) * | 2013-02-27 | 2013-08-14 | 济南华天恒达科技有限公司 | Medical microscope counting pool |
| CN203259449U (en) * | 2013-04-26 | 2013-10-30 | 江西金洹生物科技有限公司 | Flow counting chamber with variable thickness |
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