CN102174536B - 用于重组腺病毒的包装细胞 - Google Patents
用于重组腺病毒的包装细胞 Download PDFInfo
- Publication number
- CN102174536B CN102174536B CN2011100542020A CN201110054202A CN102174536B CN 102174536 B CN102174536 B CN 102174536B CN 2011100542020 A CN2011100542020 A CN 2011100542020A CN 201110054202 A CN201110054202 A CN 201110054202A CN 102174536 B CN102174536 B CN 102174536B
- Authority
- CN
- China
- Prior art keywords
- cell
- sequence
- adenovirus
- genome
- fragment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
- C12N15/861—Adenoviral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10341—Use of virus, viral particle or viral elements as a vector
- C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10351—Methods of production or purification of viral material
- C12N2710/10352—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/38—Vector systems having a special element relevant for transcription being a stuffer
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
构建体 | 用量 | 培养皿数 | 转化灶/培养皿 |
pIG.E1A.E1B | 20μgr | 2 | 31 |
pIG.E1A | 20μgr | 2 | 0 |
pIG.E1A+pE1B | 每个10μgr | 4 | 15 |
pIG.E1AB21 | 20μgr | 2 | 11.5 |
pIG.E1A B21+pE1B | 每个10μgr | 4 | 10 |
AdApt.eGFP | 20μgr | 1 | 0 |
Claims (9)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EPPCT/EP03/50679 | 2003-10-02 | ||
EP0350679 | 2003-10-02 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2004800286959A Division CN1863918B (zh) | 2003-10-02 | 2004-10-04 | 用于重组腺病毒的包装细胞 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN102174536A CN102174536A (zh) | 2011-09-07 |
CN102174536B true CN102174536B (zh) | 2013-02-13 |
Family
ID=34400432
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2004800286959A Expired - Fee Related CN1863918B (zh) | 2003-10-02 | 2004-10-04 | 用于重组腺病毒的包装细胞 |
CN2011100542020A Expired - Fee Related CN102174536B (zh) | 2003-10-02 | 2004-10-04 | 用于重组腺病毒的包装细胞 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2004800286959A Expired - Fee Related CN1863918B (zh) | 2003-10-02 | 2004-10-04 | 用于重组腺病毒的包装细胞 |
Country Status (16)
Country | Link |
---|---|
US (2) | US7816104B2 (zh) |
EP (1) | EP1670925B1 (zh) |
JP (1) | JP4749334B2 (zh) |
KR (1) | KR101203817B1 (zh) |
CN (2) | CN1863918B (zh) |
AU (1) | AU2004278517B2 (zh) |
BR (1) | BRPI0414670A (zh) |
CA (1) | CA2538025C (zh) |
DK (1) | DK1670925T3 (zh) |
EA (1) | EA010924B1 (zh) |
HK (1) | HK1084974A1 (zh) |
HR (1) | HRP20130616T1 (zh) |
IL (2) | IL174489A (zh) |
NZ (1) | NZ545501A (zh) |
SI (1) | SI1670925T1 (zh) |
WO (1) | WO2005033320A1 (zh) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6492169B1 (en) | 1999-05-18 | 2002-12-10 | Crucell Holland, B.V. | Complementing cell lines |
DK1670925T3 (da) | 2003-10-02 | 2013-07-08 | Crucell Holland Bv | Pakningsceller til rekombinat adenovirus |
US20090110695A1 (en) * | 2006-03-27 | 2009-04-30 | Menzo Jans Emko Havenga | Compositions Comprising a Recombinant Adenovirus and an Adjuvant |
WO2008134720A2 (en) * | 2007-04-30 | 2008-11-06 | Medtronic, Inc. | Inert dna sequences for efficient viral packaging and methods of use |
WO2010060719A1 (en) * | 2008-11-03 | 2010-06-03 | Crucell Holland B.V. | Method for the production of adenoviral vectors |
KR101427200B1 (ko) * | 2011-11-24 | 2014-08-07 | 주식회사 바이로메드 | 아데노바이러스 생산 신규 세포주 및 그의 용도 |
WO2013079473A1 (en) | 2011-11-28 | 2013-06-06 | Crucell Holland B.V. | Influenza virus vaccines and uses thereof |
MY169331A (en) * | 2012-03-22 | 2019-03-21 | Janssen Vaccines & Prevention Bv | Vaccine against rsv |
CN110832072B (zh) | 2017-04-21 | 2023-11-07 | 真基因太科公司 | 用于生产非复制型腺病毒的细胞系以及制备所述细胞系的方法 |
CN108342362A (zh) * | 2018-01-31 | 2018-07-31 | 武汉枢密脑科学技术有限公司 | 一种用于扩增重组犬腺病毒cav2的稳定细胞系mdck及其构建方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5994128A (en) * | 1995-06-15 | 1999-11-30 | Introgene B.V. | Packaging systems for human recombinant adenovirus to be used in gene therapy |
WO2002040665A2 (en) * | 2000-11-15 | 2002-05-23 | Crucell Holland B.V. | Complementing cell lines |
WO2003031633A2 (de) * | 2001-10-04 | 2003-04-17 | Probiogen Ag | Adenovirales vektorsystem |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4889806A (en) * | 1987-04-15 | 1989-12-26 | Washington University | Large DNA cloning system based on yeast artificial chromosomes |
US5610053A (en) | 1993-04-07 | 1997-03-11 | The United States Of America As Represented By The Department Of Health And Human Services | DNA sequence which acts as a chromatin insulator element to protect expressed genes from cis-acting regulatory sequences in mammalian cells |
US5834256A (en) * | 1993-06-11 | 1998-11-10 | Cell Genesys, Inc. | Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells |
US5820868A (en) | 1993-12-09 | 1998-10-13 | Veterinary Infectious Disease Organization | Recombinant protein production in bovine adenovirus expression vector system |
ATE336587T1 (de) | 1994-06-10 | 2006-09-15 | Genvec Inc | Adenoviren-vektor systeme und zelllinien |
ES2258265T3 (es) | 1994-11-28 | 2006-08-16 | Cell Genesys Inc. | Vectores de replicacion con especificidad tisular. |
WO1996018418A1 (en) | 1994-12-12 | 1996-06-20 | Genetic Therapy, Inc. | Improved adenoviral vectors and producer cells |
SI1161548T2 (sl) | 1999-04-15 | 2010-02-26 | Crucell Holland Bv | Priprava rekombinantnega proteina v humani celici z uporabo sekvenc, ki kodirajo adenovirusni E1 protein |
US6558948B1 (en) | 1999-11-23 | 2003-05-06 | Stefan Kochanek | Permanent amniocytic cell line, its production and use for the production of gene transfer vectors |
EP1103610A1 (en) | 1999-11-26 | 2001-05-30 | Introgene B.V. | Production of vaccines from immortalised mammalian cell lines |
DE60236331D1 (de) | 2001-07-04 | 2010-06-17 | Chromagenics Bv | DNS-Sequenzen mit Anti-Repressor-Aktivität |
US7026164B2 (en) * | 2003-07-03 | 2006-04-11 | Cell Genesys, Inc. | Adenovirus packaging cell lines |
DK1670925T3 (da) | 2003-10-02 | 2013-07-08 | Crucell Holland Bv | Pakningsceller til rekombinat adenovirus |
-
2004
- 2004-10-04 DK DK04791140.9T patent/DK1670925T3/da active
- 2004-10-04 CN CN2004800286959A patent/CN1863918B/zh not_active Expired - Fee Related
- 2004-10-04 EA EA200600693A patent/EA010924B1/ru not_active IP Right Cessation
- 2004-10-04 CN CN2011100542020A patent/CN102174536B/zh not_active Expired - Fee Related
- 2004-10-04 BR BRPI0414670-0A patent/BRPI0414670A/pt not_active Application Discontinuation
- 2004-10-04 AU AU2004278517A patent/AU2004278517B2/en not_active Ceased
- 2004-10-04 NZ NZ545501A patent/NZ545501A/en not_active IP Right Cessation
- 2004-10-04 EP EP04791140.9A patent/EP1670925B1/en active Active
- 2004-10-04 JP JP2006530273A patent/JP4749334B2/ja not_active Expired - Fee Related
- 2004-10-04 CA CA2538025A patent/CA2538025C/en not_active Expired - Fee Related
- 2004-10-04 WO PCT/EP2004/052428 patent/WO2005033320A1/en active Application Filing
- 2004-10-04 KR KR1020067006263A patent/KR101203817B1/ko active IP Right Grant
- 2004-10-04 SI SI200432052T patent/SI1670925T1/sl unknown
-
2006
- 2006-03-20 US US11/384,850 patent/US7816104B2/en not_active Expired - Fee Related
- 2006-03-22 IL IL174489A patent/IL174489A/en active IP Right Grant
- 2006-06-22 HK HK06107148.0A patent/HK1084974A1/xx not_active IP Right Cessation
-
2010
- 2010-07-14 US US12/804,179 patent/US8114637B2/en not_active Expired - Fee Related
-
2013
- 2013-07-02 HR HRP20130616TT patent/HRP20130616T1/hr unknown
- 2013-07-18 IL IL227543A patent/IL227543A/en active IP Right Grant
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5994128A (en) * | 1995-06-15 | 1999-11-30 | Introgene B.V. | Packaging systems for human recombinant adenovirus to be used in gene therapy |
WO2002040665A2 (en) * | 2000-11-15 | 2002-05-23 | Crucell Holland B.V. | Complementing cell lines |
WO2003031633A2 (de) * | 2001-10-04 | 2003-04-17 | Probiogen Ag | Adenovirales vektorsystem |
Also Published As
Publication number | Publication date |
---|---|
EA010924B1 (ru) | 2008-12-30 |
HK1084974A1 (en) | 2006-08-11 |
US20060183232A1 (en) | 2006-08-17 |
DK1670925T3 (da) | 2013-07-08 |
JP4749334B2 (ja) | 2011-08-17 |
EP1670925B1 (en) | 2013-05-01 |
IL227543A (en) | 2015-08-31 |
WO2005033320A1 (en) | 2005-04-14 |
US8114637B2 (en) | 2012-02-14 |
HRP20130616T1 (en) | 2013-08-31 |
KR101203817B1 (ko) | 2012-11-23 |
EA200600693A1 (ru) | 2006-10-27 |
SI1670925T1 (sl) | 2013-10-30 |
CA2538025C (en) | 2012-07-17 |
CN102174536A (zh) | 2011-09-07 |
CN1863918A (zh) | 2006-11-15 |
CN1863918B (zh) | 2011-03-30 |
NZ545501A (en) | 2010-12-24 |
EP1670925A1 (en) | 2006-06-21 |
AU2004278517A1 (en) | 2005-04-14 |
BRPI0414670A (pt) | 2006-11-21 |
AU2004278517B2 (en) | 2010-04-01 |
IL174489A0 (en) | 2006-08-01 |
US7816104B2 (en) | 2010-10-19 |
US20100311172A1 (en) | 2010-12-09 |
KR20060095557A (ko) | 2006-08-31 |
CA2538025A1 (en) | 2005-04-14 |
IL174489A (en) | 2013-10-31 |
JP2007507216A (ja) | 2007-03-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US9228205B2 (en) | Complementing cell lines | |
CN100374574C (zh) | 稳定的腺病毒载体及其增殖方法 | |
US8114637B2 (en) | Packaging cells for recombinant adenovirus | |
CN100497639C (zh) | 生产腺病毒载体的手段和方法 | |
Lusky et al. | In vitro and in vivo biology of recombinant adenovirus vectors with E1, E1/E2A, or E1/E4 deleted | |
CA2590943C (en) | Cell lines for production of replication-defective adenovirus | |
Dormond et al. | From the first to the third generation adenoviral vector: what parameters are governing the production yield? | |
Southgate et al. | Gene transfer into neural cells in vitro using adenoviral vectors | |
Zou et al. | ☆ DNA assembly technique simplifies the construction of infectious clone of fowl adenovirus | |
CA2461579C (en) | Porcine adenovirus e1 region | |
Ruzsics et al. | Transposon-Assisted Cloning and Traceless Mutagenesis of Adenoviruses: Development of a Novel Vector Based on SpeciesD | |
Wang et al. | Episomal segregation of the adenovirus enhancer sequence by conditional genome rearrangement abrogates late viral gene expression | |
Both | Xenogenic adenoviral vectors | |
Baxi et al. | Mutational analysis of early region 4 of bovine adenovirus type 3 | |
Wu et al. | Generation of a replication-deficient recombinant human adenovirus type 35 vector using bacteria-mediated homologous recombination | |
Li et al. | A recombinant replication-defective human adenovirus type 3: a vaccine candidate | |
Reddy et al. | Optimization of bovine coronavirus hemagglutinin-estrase glycoprotein expression in E3 deleted bovine adenovirus-3 | |
CN109762842B (zh) | 复制型重组人41型腺病毒载体系统及其应用 | |
Csaky | Preparation of recombinant adenoviruses | |
US7687615B2 (en) | PAV regions for encapsidation and E1 transcriptional control | |
Class et al. | Patent application title: COMPLEMENTING CELL LINES Inventors: Ronald Vogels (Linschoten, NL) Menzo Jans Emco Havenga (Alphen A/d Rijn, NL) Majid Mehtall (Plobsheim, FR) | |
CN102002484A (zh) | 一种腺病毒-甲病毒杂合病毒的制备及其应用 | |
Cultured | Epstein-Barr Virus Hybrid− An Adenovirus |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CI01 | Publication of corrected invention patent application |
Correction item: Inventor Name Correct: Zuijdgeest David A. T. M. False: Zuijdgeest David A. T. M. Number: 07 Volume: 29 |
|
CI03 | Correction of invention patent |
Correction item: Inventor Name Correct: Zuijdgeest David A. T. M. False: Zuijdgeest David A. T. M. Number: 07 Page: The title page Volume: 29 |
|
ERR | Gazette correction |
Free format text: CORRECT: INVENTOR NAME; FROM: DAVID A. T. M. . ZUIJDGEEST TO: DAVID A. T. M. ZUIJDGEEST |
|
RECT | Rectification | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130213 Termination date: 20211004 |
|
CF01 | Termination of patent right due to non-payment of annual fee |