CN101391862B - Biological chip glass slide and preparation thereof - Google Patents

Biological chip glass slide and preparation thereof Download PDF

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Publication number
CN101391862B
CN101391862B CN200710132686XA CN200710132686A CN101391862B CN 101391862 B CN101391862 B CN 101391862B CN 200710132686X A CN200710132686X A CN 200710132686XA CN 200710132686 A CN200710132686 A CN 200710132686A CN 101391862 B CN101391862 B CN 101391862B
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percent
weight percent
glass slide
biological chip
chip glass
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CN200710132686XA
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CN101391862A (en
Inventor
吴堂明
潘能科
陆冬雷
张必达
江晨亮
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Jiangsu Sanlian Bioengineering Co.,Ltd.
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JIANGSU SANLIAN BIOENGINEERING CO Ltd
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Abstract

The invention discloses a glass slide of a biochip and a manufacture method thereof. The technical proposal is as follows: the glass slide of the biochip consists of the following ingredients by weight percentage: 44.7 percent to 50.9 percent of quartz sand, 28.9 percent to 46.25 percent of sodium carbonate, 0.1 percent to 12 percent of MnO2, 1 percent to 5 percent of borax, 0.1 percent to 10 percent of NaNO3, 0.1 percent to 2 percent of carbon powder, 0.1 percent to 2 percent of arsenic trioxide and 0.1 percent to 2 percent of K2CO3. The manufacture method of the glass slide of the biochip comprises the following steps: (1) under the condition of room temperature, the quartz sand and the sodium carbonate are mixed to be uniform firstly; (2) MnO2, the borax, NaNO3, the carbon powder, the arsenic trioxide and K2CO3 are added and stirred to be uniform; (3) the obtained mixture is thrown into a furnace, is heated, melted into slurry and stirred continuously for 1 hour; (4) the temperatureis fast cooled to 25 to 40 DEG C; (5) slicing is carried out; and (6) polishing is carried out and then the glass slide of the biochip is formed. The film base of the background value of the glass slide is superior to the film base of the common glass slide obviously, and as to the signal strength, the average signal strength of the glass slide is superior to the common glass slide.

Description

Biological chip glass carrier and preparation method thereof
Technical field
The present invention relates to a kind of biological chip glass carrier and preparation method thereof.
Background technology
The present biological chip glass carrier of making, normal based on transparent slide glass and quartzy slide glass, though being used for the biochip making, these two kinds of slide glasss can accomplish good homogeneity, but shortcoming is also clearly, one, be difficult to reach very low background (also is to obtain even if can reach) under the prerequisite of sacrificing echo signal intensity; Two, frame effect obvious (chemoluminescence ELISA) influences the analysis of edge data.
Summary of the invention
Technical problem to be solved by this invention provides biological chip glass carrier of a kind of shortcoming that can overcome prior art and preparation method thereof.
Technical scheme of the present invention comprises that biological chip glass carrier is made up of following component by weight percentage:
The quartz sand of 44.7%-50.9%, the yellow soda ash of 28.9%-46.25%, the MnO of 0.1-12% 2, the borax of 1-5%, the NaNO of 0.1-10% 3, the carbon dust of 0.1-2%, the white arsenic of 0.1-2%, the K of 0.1-2% 2CO 3
The quartz sand weight percent also can be 46.5%-48.5%, and the yellow soda ash weight percent also can be 35%-40%, MnO 2The micro mist weight percent also can be 2-6%, and the borax weight percent also can be 2-4%, NaNO 3Weight percent also can be 2-6%, and the carbon dust weight percent also can be 0.5-1%, and the white arsenic weight percent also can be 0.5-1%, K 2CO 3Weight percent also can be 0.5-1%.
The making of above-mentioned biological chip glass carrier comprises the steps:
(1) under the room temperature condition, earlier with quartz sand and yellow soda ash mixing;
(2) add MnO2, borax, NaNO3, carbon dust, white arsenic, K2CO3, stirring and evenly mixing;
(3) above-mentioned compound is dropped into smelting furnace, heating is melt into pulpous state, and continuously stirring 1 hour;
(4) be cooled to 25-40 ℃ rapidly;
(5) section;
(6) polishing, moulding.
Beneficial effect
In order to overcome the negative impact of transparent slide glass, guaranteeing under the constant prerequisite of slide glass binding site, the present invention is implemented in and changes the light transmission of glass to reach the effect that reduces background and eliminate the frame effect, the MnO of purple when guaranteeing enough binding abilities by add special compound in the glass of high temperature melting 2Greatly reduce the light transmission of glass, reach the purpose that reduces background and eliminate the frame effect, and quartz sand, the add-on of yellow soda ash directly influences the binding site of glass surface, NaNO 3, white arsenic has then guaranteed the homogeneity of glass.
The biochip frame effect that common slide glass is made is obvious, and the point data of the edge that keeps to the side can't be analyzed; And of the present invention base do not have the frame effect substantially, and advantage is obvious.The background aspect, the average common slide glass of background values is about 70, sheet base 55 of the present invention, the background values of sheet base of the present invention obviously is better than common slide glass sheet base.React under identical conditions the strength of signal aspect, and common slide glass average signal strength is about 850, and slide glass average signal strength of the present invention is better than common slide glass about 900.
Embodiment
By embodiment and test result technical scheme of the present invention is further described.
Embodiment 1
(1) under the room temperature condition, earlier be that 47.8% quartz sand and weight percent are 28.9% yellow soda ash mixing with weight percent;
(2) add 12% MnO2,1% borax, 10% NaNO3,0.1% carbon dust, 0.1% white arsenic, 0.1% K2CO3, stirring and evenly mixing again;
(3) above-mentioned compound is dropped into smelting furnace, heating is melt into pulpous state, and continuously stirring 1 hour;
(4) be cooled to 30 ℃ rapidly;
(5) section;
(6) polishing, moulding.
Embodiment 2
(1) under the room temperature condition, earlier be that 50.9% quartz sand and weight percent are 30.6% yellow soda ash mixing with weight percent;
(2) add 10% MnO2,2% borax, 5% NaNO3,0.5% carbon dust, 0.5% white arsenic, 0.5% K2CO3, stirring and evenly mixing again;
(3) above-mentioned compound is dropped into smelting furnace, heating is melt into pulpous state, and continuously stirring 1 hour;
(4) be cooled to 35 ℃ rapidly;
(5) section;
(6) polishing, moulding.
Embodiment 3
(1) under the room temperature condition, earlier be that 50.9% quartz sand and weight percent are 30.6% yellow soda ash mixing with weight percent;
(2) MnO2 of adding 8%, 5% borax, 2.5% NaNO3,1% carbon dust, 1% white arsenic, 1% K2CO3, stirring and evenly mixing;
(3) above-mentioned compound is dropped into smelting furnace, heating is melt into pulpous state, and continuously stirring 1 hour;
(4) be cooled to 40 ℃ rapidly;
(5) section;
(6) polishing, moulding.
Embodiment 4
(1) under the room temperature condition, earlier be that 48.2% quartz sand and weight percent are 38.7% yellow soda ash mixing with weight percent;
(2) add 6% MnO2,1% borax, 0.1% NaNO3,2% carbon dust, 2% white arsenic, 2% K2CO3, stirring and evenly mixing again;
(3) above-mentioned compound is dropped into smelting furnace, heating is melt into pulpous state, and continuously stirring 1 hour;
(4) be cooled to 25 ℃ rapidly;
(5) section;
(6) polishing, moulding.
Embodiment 5
(1) under the room temperature condition, earlier be that 46.5% quartz sand and weight percent are 37.2% yellow soda ash mixing with weight percent;
(2) add 4% MnO2,2% borax, 10% NaNO3,0.1% carbon dust, 0.1% white arsenic, 0.1% K2CO3, stirring and evenly mixing again;
(3) above-mentioned compound is dropped into smelting furnace, heating is melt into pulpous state, and continuously stirring 1 hour;
(4) be cooled to 28 ℃ rapidly;
(5) section;
(6) polishing, moulding.
Embodiment 6
(1) under the room temperature condition, earlier be that 48.3% quartz sand and weight percent are 38.7% yellow soda ash mixing with weight percent;
(2) add 2% MnO2,5% borax, 5% NaNO3,0.1% carbon dust, 0.5% white arsenic, 0.5% K2CO3, stirring and evenly mixing again;
(3) above-mentioned compound is dropped into smelting furnace, heating is melt into pulpous state, and continuously stirring 1 hour;
(4) be cooled to 30 ℃ rapidly;
(5) section;
(6) polishing, moulding.
Embodiment 7
(1) under the room temperature condition, earlier be that 46.25% quartz sand and weight percent are 46.25% yellow soda ash mixing with weight percent;
(2) add 1% MnO2,1% borax, 2.5% NaNO3,1% carbon dust, 1% white arsenic, 1% K2CO3, stirring and evenly mixing again;
(3) above-mentioned compound is dropped into smelting furnace, heating is melt into pulpous state, and continuously stirring 1 hour;
(4) be cooled to 27 ℃ rapidly;
(5) section;
(6) polishing, moulding.
Embodiment 8
(1) under the room temperature condition, earlier be that 45.7% quartz sand and weight percent are 45.7% yellow soda ash mixing with weight percent;
(2) MnO2 of adding 0.5%, 2% borax, 0.1% NaNO3,2% carbon dust, 2% white arsenic, 2% K2CO3, stirring and evenly mixing;
(3) above-mentioned compound is dropped into smelting furnace, heating is melt into pulpous state, and continuously stirring 1 hour;
(4) be cooled to 38 ℃ rapidly;
(5) section;
(6) polishing, moulding.
Embodiment 9
(1) under the room temperature condition, earlier be that 44.7% quartz sand and weight percent are 44.7% yellow soda ash mixing with weight percent;
(2) MnO2 of adding 0.1%, 5% borax, 5% NaNO3,0.5% carbon dust, 0.1% white arsenic, 0.2% K2CO3, stirring and evenly mixing;
(3) above-mentioned compound is dropped into smelting furnace, heating is melt into pulpous state, and continuously stirring 1 hour;
(4) be cooled to 32 ℃ rapidly;
(5) section;
(6) polishing, moulding.
Following table 1 is the part of detecting result of embodiment of the invention 1-9
Table 1: test result
The raw material that is used in the table test is respectively:
Ankyrin: AFP (Alpha-Fetoprotein) antibody (the rich match in Henan biological production)
Signal antibody: horseradish peroxidase-labeled AFP antibody (the rich match in Henan biological production)
Encapsulant: calf serum (the general imperial biological production in Shanghai)
Reaction washing lotion: 0.1mol/l Tris-Hcl (biological production of U.S. season of Shanghai)
Luminous substrate: SuperSignal ELISA Femto Maximum Sensitivity Substrate (U.S. pierce production)
Effect of the present invention as can be seen from the table is good, and not only signal is strong, and background values is low, does not have the frame effect substantially, is very suitable for the making of biochip.

Claims (10)

1. biological chip glass carrier, form by following component by weight percentage:
The quartz sand of 44.7%-50.9%, the yellow soda ash of 28.9%-46.25%, the MnO of 0.1-12% 2, the borax of 1-5%, the NaNO of 0.1-10% 3, the carbon dust of 0.1-2%, the white arsenic of 0.1-2%, the K of 0.1-2% 2CO 3,
It makes as follows:
(1) under the room temperature condition, with quartz sand and yellow soda ash mixing;
(2) add MnO 2, borax, NaNO 3, carbon dust, white arsenic, K 2CO 3, stirring and evenly mixing;
(3) above-mentioned compound is dropped into smelting furnace, heating is melt into pulpous state, and continuously stirring 1 hour;
(4) be cooled to 25-40 ℃ rapidly;
(5) section;
(6) polishing, moulding.
2. biological chip glass carrier according to claim 1 is characterized in that: the quartz sand weight percent is 46.5%-48.5%.
3. biological chip glass carrier according to claim 1 is characterized in that: the yellow soda ash weight percent is 35%-40%.
4. biological chip glass carrier according to claim 1 is characterized in that: MnO 2Weight percent is 2-6%.
5. biological chip glass carrier according to claim 1 is characterized in that: the borax weight percent is 2-4%.
6. biological chip glass carrier according to claim 1 is characterized in that: NaNO 3Weight percent is 2-6%.
7. biological chip glass carrier according to claim 1 is characterized in that: the carbon dust weight percent is 0.5-1%.
8. biological chip glass carrier according to claim 1 is characterized in that: the white arsenic weight percent is 0.5-1%.
9. biological chip glass carrier according to claim 1 is characterized in that: K 2CO 3Weight percent is 0.5-1%.
10. the making method of a biological chip glass carrier according to claim 1, it comprises the steps:
(1) under the room temperature condition, with quartz sand and yellow soda ash mixing;
(2) add MnO 2, borax, NaNO 3, carbon dust, white arsenic, K 2CO 3, stirring and evenly mixing;
(3) above-mentioned compound is dropped into smelting furnace, heating is melt into pulpous state, and continuously stirring 1 hour;
(4) be cooled to 25-40 ℃ rapidly;
(5) section;
(6) polishing, moulding.
CN200710132686XA 2007-09-18 2007-09-18 Biological chip glass slide and preparation thereof Active CN101391862B (en)

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CN101391862B true CN101391862B (en) 2010-06-09

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102775064A (en) * 2012-02-16 2012-11-14 湖北晶彩玻璃有限公司 Production recipe of purple true color glass bottle

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3647506A (en) * 1967-07-14 1972-03-07 Forschungsgemeinschaft Fur Tec Method of and apparatus for burning graduations symbols and lettering into glass articles
US5446007A (en) * 1994-05-10 1995-08-29 Schott Glass Technologies, Inc. Brown contrast enhancement glass
CN1263161A (en) * 1999-12-29 2000-08-16 中国科学院上海冶金研究所 cDNA microarray chip, preparation method and its application
CN1569707A (en) * 2004-05-01 2005-01-26 张红俭 Novel wine bottle and its preparing process
CN1935723A (en) * 2005-09-20 2007-03-28 徐志国 Solution for increasing adhesion and affinity of glass slide

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3647506A (en) * 1967-07-14 1972-03-07 Forschungsgemeinschaft Fur Tec Method of and apparatus for burning graduations symbols and lettering into glass articles
US5446007A (en) * 1994-05-10 1995-08-29 Schott Glass Technologies, Inc. Brown contrast enhancement glass
CN1263161A (en) * 1999-12-29 2000-08-16 中国科学院上海冶金研究所 cDNA microarray chip, preparation method and its application
CN1569707A (en) * 2004-05-01 2005-01-26 张红俭 Novel wine bottle and its preparing process
CN1935723A (en) * 2005-09-20 2007-03-28 徐志国 Solution for increasing adhesion and affinity of glass slide

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Denomination of invention: Biological chip glass carrier and preparation thereof

Effective date of registration: 20190726

Granted publication date: 20100609

Pledgee: Agricultural Bank of China Limited by Share Ltd Wuxi science and Technology Branch

Pledgor: Jiangsu Sanlian Bioengineering Co., Ltd.

Registration number: 2019990000792

PC01 Cancellation of the registration of the contract for pledge of patent right
PC01 Cancellation of the registration of the contract for pledge of patent right

Date of cancellation: 20200810

Granted publication date: 20100609

Pledgee: Agricultural Bank of China Limited by Share Ltd. Wuxi science and Technology Branch

Pledgor: SUNLANT BIOLOGICAL ENGINEERING Co.,Ltd.

Registration number: 2019990000792

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CP03 Change of name, title or address

Address after: No. 35-305, Changjiang South Road, Xinwu District, Wuxi, Jiangsu 214111

Patentee after: Jiangsu Sanlian Bioengineering Co.,Ltd.

Address before: 214101 Wuxi New District, Jiangsu Province, Xingyuan South Road, Southeast University, Wuxi Campus

Patentee before: SUNLANT BIOLOGICAL ENGINEERING CO.,LTD.