CA2401727A1 - Thermophilic dna polymerases from thermoactinomyces vulgaris - Google Patents

Thermophilic dna polymerases from thermoactinomyces vulgaris Download PDF

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Publication number
CA2401727A1
CA2401727A1 CA002401727A CA2401727A CA2401727A1 CA 2401727 A1 CA2401727 A1 CA 2401727A1 CA 002401727 A CA002401727 A CA 002401727A CA 2401727 A CA2401727 A CA 2401727A CA 2401727 A1 CA2401727 A1 CA 2401727A1
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CA
Canada
Prior art keywords
dna polymerases
present
vulgaris
thermoactinomyces vulgaris
thermophilic dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002401727A
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French (fr)
Other versions
CA2401727C (en
Inventor
Trent Gu
Fen Huang
James Robert Hartnett
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corporation
Trent Gu
Fen Huang
James Robert Hartnett
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corporation, Trent Gu, Fen Huang, James Robert Hartnett filed Critical Promega Corporation
Publication of CA2401727A1 publication Critical patent/CA2401727A1/en
Application granted granted Critical
Publication of CA2401727C publication Critical patent/CA2401727C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase

Abstract

The present invention provides compositions comprising thermostable DNA
polymerases derived from hyperthermophilic eubacteria. In particular, the present invention comprises thermostable DNA
polymerases from the hyperthermophilic eubacterial species Thermoactinomyces vulgaris. The present invention also provides methods for utilizing naturally-occurring and non-naturally-occurring forms of T. vulgaris DNA polymerase in sequencing, reverse transcription, and amplification reactions.
CA002401727A 2000-03-02 2001-02-28 Thermophilic dna polymerases from thermoactinomyces vulgaris Expired - Fee Related CA2401727C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/517,439 US6632645B1 (en) 2000-03-02 2000-03-02 Thermophilic DNA polymerases from Thermoactinomyces vulgaris
US09/517,439 2000-03-02
PCT/US2001/006436 WO2001064838A2 (en) 2000-03-02 2001-02-28 Thermophilic dna polymerases from thermoactinomyces vulgaris

Publications (2)

Publication Number Publication Date
CA2401727A1 true CA2401727A1 (en) 2001-09-07
CA2401727C CA2401727C (en) 2009-04-28

Family

ID=24059801

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002401727A Expired - Fee Related CA2401727C (en) 2000-03-02 2001-02-28 Thermophilic dna polymerases from thermoactinomyces vulgaris

Country Status (6)

Country Link
US (3) US6632645B1 (en)
EP (1) EP1259529A4 (en)
JP (1) JP2003525603A (en)
AU (2) AU4185501A (en)
CA (1) CA2401727C (en)
WO (1) WO2001064838A2 (en)

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JP2008500818A (en) * 2004-04-06 2008-01-17 ストラタジーン カリフォルニア Compositions and methods for reverse transcription
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7772390B1 (en) 2006-07-18 2010-08-10 The Regents Of The University Of California Lipid mediated nucleic acid synthesis
CN101948853B (en) * 2010-09-07 2012-01-11 广州华峰生物科技有限公司 Thermophilic fat bacillus DNA polymerase
WO2012146260A1 (en) * 2011-04-23 2012-11-01 Biolytix Ag Production and use of proteins in molecular biology
US9447388B2 (en) * 2011-07-12 2016-09-20 Kyushu University, National University Corporation DNA polymerases
WO2014161712A1 (en) * 2013-04-05 2014-10-09 Bioron Gmbh Novel dna-ploymerases

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Also Published As

Publication number Publication date
AU2001241855B2 (en) 2005-07-07
WO2001064838A2 (en) 2001-09-07
US20070082333A1 (en) 2007-04-12
EP1259529A4 (en) 2005-04-13
AU4185501A (en) 2001-09-12
WO2001064838A3 (en) 2002-03-07
EP1259529A2 (en) 2002-11-27
CA2401727C (en) 2009-04-28
JP2003525603A (en) 2003-09-02
US6632645B1 (en) 2003-10-14
US20070172879A1 (en) 2007-07-26
US7214522B2 (en) 2007-05-08

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