CA2244964A1 - Detection of nucleic acids by fluorescence quenching - Google Patents

Detection of nucleic acids by fluorescence quenching

Info

Publication number
CA2244964A1
CA2244964A1 CA002244964A CA2244964A CA2244964A1 CA 2244964 A1 CA2244964 A1 CA 2244964A1 CA 002244964 A CA002244964 A CA 002244964A CA 2244964 A CA2244964 A CA 2244964A CA 2244964 A1 CA2244964 A1 CA 2244964A1
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CA
Canada
Prior art keywords
oligonucleotide
donor
stranded
partially
oligonucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002244964A
Other languages
French (fr)
Other versions
CA2244964C (en
Inventor
James G. Nadeau
Helen V. Hsieh
J. Bruce Pitner
C. Preston Linn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
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Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Publication of CA2244964A1 publication Critical patent/CA2244964A1/en
Application granted granted Critical
Publication of CA2244964C publication Critical patent/CA2244964C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Saccharide Compounds (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Detector nucleic acids are employed for detection of nucleic acid target sequences by fluorescence quenching mechanisms. The detector nucleic acid comprises at least two oligonucleotides and is partially single-stranded and partially double-stranded. One of the two dyes of a donor/acceptor dye pair is linked to the first oligonucleotide and the other is linked to a second oligonucleotide such that they are in close spatial proximity when the first and second oligonucleotides are base-paired and donor fluorescence is quenched. A single second oligonucleotide may be hybridized to the first oligonucleotide or multiple second oligonucleotides may be hybridized to the first oligonucleotide and to each other, forming a junction structure comprising multiple donor/acceptor dye pairs. The detector oligonucleotide retains its partially single-stranded and partially double-stranded conformation in the absence of target. In the presence of target, however, the second oligonucleotide(s) of the detector nucleic acid is/are completely or partially displaced from the first, increasing the distance between the donor and acceptor dyes and causing a change in fluorescence which may be detected as an indication of the presence of the target sequence.
CA002244964A 1997-09-23 1998-09-22 Detection of nucleic acids by fluorescence quenching Expired - Fee Related CA2244964C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/933,749 US5935791A (en) 1997-09-23 1997-09-23 Detection of nucleic acids by fluorescence quenching
US08/933,749 1997-09-23

Publications (2)

Publication Number Publication Date
CA2244964A1 true CA2244964A1 (en) 1999-03-23
CA2244964C CA2244964C (en) 2002-12-31

Family

ID=25464442

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002244964A Expired - Fee Related CA2244964C (en) 1997-09-23 1998-09-22 Detection of nucleic acids by fluorescence quenching

Country Status (5)

Country Link
US (3) US5935791A (en)
EP (1) EP0909823B1 (en)
JP (1) JPH11155598A (en)
CA (1) CA2244964C (en)
DE (1) DE69836885T2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6815164B2 (en) 2000-10-06 2004-11-09 Nugen Technologies, Inc. Methods and probes for detection and/or quantification of nucleic acid sequences
US7858302B2 (en) 2002-03-12 2010-12-28 Enzo Life Sciences, Inc. Processes for incorporating nucleic acid sequences into an analyte or library of analytes
CN105803074A (en) * 2016-04-12 2016-07-27 浙江大学 Primer-type nucleic acid fluorescent probe subjected to two-way strand displacement
US10144957B2 (en) 2002-03-12 2018-12-04 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes

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US8247179B2 (en) 2002-03-12 2012-08-21 Enzo Life Sciences, Inc. Processes for quantitative or qualitative detection of single-stranded nucleic acids
US10144957B2 (en) 2002-03-12 2018-12-04 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
CN105803074A (en) * 2016-04-12 2016-07-27 浙江大学 Primer-type nucleic acid fluorescent probe subjected to two-way strand displacement

Also Published As

Publication number Publication date
CA2244964C (en) 2002-12-31
DE69836885T2 (en) 2007-06-14
EP0909823A3 (en) 2001-12-12
EP0909823B1 (en) 2007-01-17
EP0909823A2 (en) 1999-04-21
US5935791A (en) 1999-08-10
US6130047A (en) 2000-10-10
JPH11155598A (en) 1999-06-15
DE69836885D1 (en) 2007-03-08
US6261784B1 (en) 2001-07-17

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