CA2240609A1 - A method for generating hypermutable organisms - Google Patents

A method for generating hypermutable organisms Download PDF

Info

Publication number
CA2240609A1
CA2240609A1 CA002240609A CA2240609A CA2240609A1 CA 2240609 A1 CA2240609 A1 CA 2240609A1 CA 002240609 A CA002240609 A CA 002240609A CA 2240609 A CA2240609 A CA 2240609A CA 2240609 A1 CA2240609 A1 CA 2240609A1
Authority
CA
Canada
Prior art keywords
organisms
cells
genes
generating hypermutable
hypermutable organisms
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002240609A
Other languages
French (fr)
Other versions
CA2240609C (en
Inventor
Nicholas Nicolaides
Bert Vogelstein
Kenneth W. Kinzler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Johns Hopkins University
Original Assignee
Johns Hopkins University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johns Hopkins University filed Critical Johns Hopkins University
Publication of CA2240609A1 publication Critical patent/CA2240609A1/en
Application granted granted Critical
Publication of CA2240609C publication Critical patent/CA2240609C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)

Abstract

Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into cells and transgenic animals, new cell lines and animal varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation.
CA002240609A 1998-04-14 1998-08-11 A method for generating hypermutable organisms Expired - Lifetime CA2240609C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/059,461 US6146894A (en) 1998-04-14 1998-04-14 Method for generating hypermutable organisms
US09/059,461 1998-04-14

Publications (2)

Publication Number Publication Date
CA2240609A1 true CA2240609A1 (en) 1999-10-14
CA2240609C CA2240609C (en) 2009-02-03

Family

ID=22023099

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002240609A Expired - Lifetime CA2240609C (en) 1998-04-14 1998-08-11 A method for generating hypermutable organisms

Country Status (2)

Country Link
US (3) US6146894A (en)
CA (1) CA2240609C (en)

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001062945A1 (en) * 2000-02-23 2001-08-30 The Johns Hopkins University Methods for generating hypermutable yeast
WO2002024890A2 (en) * 2000-09-18 2002-03-28 Pioneer Hi-Bred International, Inc. Rice mlh1 ortholog and uses thereof
WO2002038750A1 (en) 2000-11-07 2002-05-16 Morphotek Inc. Methods for isolating novel antimicrobial agents from hypermutable mammalian cells
WO2002037967A1 (en) 2000-11-07 2002-05-16 Morphotek Inc. Methods for generating genetically altered antibody-producing cell lines with improved antibody characteristics
US6576468B1 (en) 2000-11-07 2003-06-10 Morphotek, Inc. Methods for obtaining microbe-resistant mammalian cells from hypermutable mammalian cells
EP1363930A1 (en) * 2000-11-14 2003-11-26 Morphotek Inc. A method for generating genetically altered antigens
WO2001088192A3 (en) * 2000-05-12 2004-02-26 Univ Johns Hopkins A method for generating hypermutable organisms
US6737268B1 (en) 2000-11-14 2004-05-18 Morphotek, Inc. Method for generating genetically altered antigens
US6808894B1 (en) 2000-11-07 2004-10-26 Morphotek, Inc. Methods for generating genetically altered antibody producing cell lines with improved antibody characteristics
US6982169B2 (en) 2001-01-15 2006-01-03 Morphotek, Inc. Chemical inhibitors of mismatch repair
US7026119B2 (en) 2000-02-11 2006-04-11 Morphotek, Inc. Methods for generating hypermutable microbes
US7223598B2 (en) 2000-11-07 2007-05-29 Morphotek, Inc. Methods for isolating novel antimicrobial agents from hypermutable mammalian cells
US7235643B2 (en) 2000-11-07 2007-06-26 Morphotek, Inc. Antibodies and methods for generating genetically altered antibodies with high affinity
EP1282708B1 (en) * 2000-02-18 2008-07-02 Morphotek, Inc. A method for generating hypermutable plants
US7754450B2 (en) 2002-11-15 2010-07-13 Morphotek, Inc. Methods of generating high-production of antibodies from hybridomas created by in vitro immunization
US7807416B2 (en) 2003-07-29 2010-10-05 Morphotek, Inc. Antibodies and methods for generating genetically altered antibodies with enhanced effector function

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6146894A (en) * 1998-04-14 2000-11-14 The Johns Hopkins University Method for generating hypermutable organisms
US7122339B2 (en) * 1998-10-09 2006-10-17 Medical Research Council Method for generating diversity
EP1430123B1 (en) * 2000-05-17 2018-01-03 The Johns Hopkins University A method for generating hypermutable organisms
JP5036119B2 (en) * 2001-01-15 2012-09-26 モーフオテク・インコーポレーテツド Chemical inhibitors of mismatch repair
WO2002059331A1 (en) * 2001-01-24 2002-08-01 Novozymes A/S In vivo mutation and recombination in filamentous fungi
EP1417329A4 (en) * 2001-07-25 2005-05-11 Morphotek Inc Methods for generating antibiotic resistant microbes and novel antibiotics
US20030068808A1 (en) * 2001-07-25 2003-04-10 Nicolaides Nicholas C. Methods for generating antibiotic resistant microbes and novel antibiotics
WO2003031937A2 (en) * 2001-10-12 2003-04-17 Morphotek, Inc. Genetic hypermutability of plants for gene discovery and diagnosis
EP1474522B1 (en) * 2002-01-18 2012-03-07 Morphotek, Inc. A method for generating engineered cells for locus specific gene regulation and analysis
CA2476480A1 (en) * 2002-02-21 2003-09-04 Morphotek Inc. Methods of making hypermutable cells using pmsr homologs
EP1476577B1 (en) * 2002-02-21 2009-04-15 Morphotek Inc. Regulated vectors for controlling dna hypermutability in eukaryotic cells
WO2003104451A2 (en) * 2002-06-06 2003-12-18 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Modifying dna recombination and repair
CA2493679A1 (en) * 2002-07-19 2004-01-29 Morphotek, Inc. Methods for generating enhanced antibody-producing cell lines with improved growth characteristics
EP1626991A2 (en) * 2003-05-23 2006-02-22 Morphotek, Inc. Anti alpha-folate-receptor-tetramer antibodies
US7332584B2 (en) * 2003-12-08 2008-02-19 Morphotek, Inc. Antibodies that specifically bind PMS2
AU2005214331B2 (en) 2004-02-12 2011-09-15 Eisai, Inc. Monoclonal antibodies that specifically bind to folate receptor alpha
AU2005311660B2 (en) * 2004-12-03 2011-07-07 Morphotek, Inc. Use of endosialin binding proteins to isolate endosialin positive cells
AU2006223301B2 (en) * 2005-03-10 2010-11-04 Eisai, Inc. Anti-mesothelin antibodies
CA2607444C (en) * 2005-04-22 2015-03-10 Morphotek, Inc. Antibodies with immune effector activity and that internalize in folate receptor alpha-positive cells
AU2006241235B2 (en) * 2005-04-22 2012-04-05 Eisai, Inc. Antibodies with immune effector activity and that internalize in endosialin-positive cells
WO2007121396A2 (en) * 2006-04-17 2007-10-25 Morphotek, Inc. Whole genome evolution technology applied to improve protein and antibody yields by cells
US20090068741A1 (en) * 2007-01-17 2009-03-12 Invitrogen Corporation Methods and Compositions for Improving the Health of Cells in Culture
EP2126105A4 (en) 2007-02-20 2010-11-03 Anaptysbio Inc Somatic hypermutation systems
SI2137217T1 (en) 2007-04-05 2014-07-31 Morphotek, Inc. Methods for inhibiting the binding of endosialin to ligands
EP2257572A1 (en) * 2008-03-27 2010-12-08 The Government of the United States of America as represented by The Secretary of the Department of Health and Human Services Human anti-mesothelin monoclonal antibodies
WO2009124109A1 (en) 2008-04-04 2009-10-08 The Government Of The U.S. A. As Represented By The Secretary Of The Dept. Of Health &Human Services Human monoclonal antibodies specific for cd22
EP2814969A4 (en) * 2012-02-10 2016-02-17 Univ Minnesota Dna assimilation

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6191268B1 (en) 1993-12-17 2001-02-20 Dana-Farber Cancer Institute Compositions and methods relating to DNA mismatch repair genes
US5843757A (en) 1995-08-24 1998-12-01 The Johns Hopkins University Human JTV1 gene overlaps PMS2 gene
US5907079A (en) 1996-01-18 1999-05-25 Amgen Canada Inc. MSH2 disrupted mice develop lymphomas
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
AUPO974597A0 (en) 1997-10-10 1997-11-06 Rhone-Poulenc Agro Methods for obtaining plant varieties
US6146894A (en) 1998-04-14 2000-11-14 The Johns Hopkins University Method for generating hypermutable organisms

Cited By (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7026119B2 (en) 2000-02-11 2006-04-11 Morphotek, Inc. Methods for generating hypermutable microbes
US7892832B2 (en) 2000-02-11 2011-02-22 The Johns Hopkins University Methods for generating hypermutable microbes
US7695969B2 (en) 2000-02-11 2010-04-13 The Johns Hopkins University Methods for generating hypermutable microbes
EP1282708B1 (en) * 2000-02-18 2008-07-02 Morphotek, Inc. A method for generating hypermutable plants
US6921666B2 (en) 2000-02-23 2005-07-26 The Johns Hopkins University Methods for generating hypermutable yeast
US7759121B2 (en) 2000-02-23 2010-07-20 The John Hopkins University Methods for generating hypermutable yeast
US7514216B2 (en) 2000-02-23 2009-04-07 The Johns Hopkins University Methods for generating hypermutable yeast
US6656736B2 (en) 2000-02-23 2003-12-02 The Johns Hopkins University Methods for generating hypermutable yeast
WO2001062945A1 (en) * 2000-02-23 2001-08-30 The Johns Hopkins University Methods for generating hypermutable yeast
US6825038B2 (en) 2000-05-12 2004-11-30 The Johns Hopkins University Method for generating hypermutable organisms
WO2001088192A3 (en) * 2000-05-12 2004-02-26 Univ Johns Hopkins A method for generating hypermutable organisms
US7319036B2 (en) 2000-05-12 2008-01-15 The Johns Hopkins University School Of Medicine Method for generating hypermutable organisms
WO2002024890A2 (en) * 2000-09-18 2002-03-28 Pioneer Hi-Bred International, Inc. Rice mlh1 ortholog and uses thereof
WO2002024890A3 (en) * 2000-09-18 2003-01-30 Pioneer Hi Bred Int Rice mlh1 ortholog and uses thereof
EP1343879A4 (en) * 2000-11-07 2006-03-15 Morphotek Inc Methods for isolating novel antimicrobial agents from hypermutable mammalian cells
US6576468B1 (en) 2000-11-07 2003-06-10 Morphotek, Inc. Methods for obtaining microbe-resistant mammalian cells from hypermutable mammalian cells
JP2005502302A (en) * 2000-11-07 2005-01-27 モーフオテク・インコーポレーテツド Method for isolating novel antimicrobial agents from hypermutable mammalian cells
US7939324B2 (en) 2000-11-07 2011-05-10 Morphotek, Inc. Methods for isolating novel antimicrobial agents from hypermutable mammalian cells
JP2004529613A (en) * 2000-11-07 2004-09-30 モーフオテク・インコーポレーテツド Method for generating a genetically modified antibody producing cell line with improved antibody properties
EP1345495A4 (en) * 2000-11-07 2005-08-17 Morphotek Inc Methods for generating genetically altered antibody-producing cell lines with improved antibody characteristics
EP1343879A1 (en) * 2000-11-07 2003-09-17 Morphotek Inc. Methods for isolating novel antimicrobial agents from hypermutable mammalian cells
US7671179B2 (en) 2000-11-07 2010-03-02 Morphotek, Inc. Antibodies and methods for generating genetically altered antibodies with high affinity
EP1345495A1 (en) * 2000-11-07 2003-09-24 Morphotek Inc. Methods for generating genetically altered antibody-producing cell lines with improved antibody characteristics
US7223598B2 (en) 2000-11-07 2007-05-29 Morphotek, Inc. Methods for isolating novel antimicrobial agents from hypermutable mammalian cells
US7235643B2 (en) 2000-11-07 2007-06-26 Morphotek, Inc. Antibodies and methods for generating genetically altered antibodies with high affinity
US6808894B1 (en) 2000-11-07 2004-10-26 Morphotek, Inc. Methods for generating genetically altered antibody producing cell lines with improved antibody characteristics
WO2002037967A1 (en) 2000-11-07 2002-05-16 Morphotek Inc. Methods for generating genetically altered antibody-producing cell lines with improved antibody characteristics
EP2039251A1 (en) * 2000-11-07 2009-03-25 Morphotek, Inc. Methods for generating genetically altered antibody-producing cell lines with improved antibody characteristics
WO2002038750A1 (en) 2000-11-07 2002-05-16 Morphotek Inc. Methods for isolating novel antimicrobial agents from hypermutable mammalian cells
EP1363930A4 (en) * 2000-11-14 2004-04-14 Morphotek Inc A method for generating genetically altered antigens
EP1363930A1 (en) * 2000-11-14 2003-11-26 Morphotek Inc. A method for generating genetically altered antigens
US6737268B1 (en) 2000-11-14 2004-05-18 Morphotek, Inc. Method for generating genetically altered antigens
JP2005509399A (en) * 2000-11-14 2005-04-14 モーフオテク・インコーポレーテツド Generating a genetically modified antigen
US6982169B2 (en) 2001-01-15 2006-01-03 Morphotek, Inc. Chemical inhibitors of mismatch repair
US8105836B2 (en) 2001-01-15 2012-01-31 Morphotek, Inc. Chemical inhibitors of mismatch repair
US7754450B2 (en) 2002-11-15 2010-07-13 Morphotek, Inc. Methods of generating high-production of antibodies from hybridomas created by in vitro immunization
US8445229B2 (en) 2002-11-15 2013-05-21 Morphotek, Inc. Methods of generating high-production of antibodies from hybridomas created by in vitro immunization
US7807416B2 (en) 2003-07-29 2010-10-05 Morphotek, Inc. Antibodies and methods for generating genetically altered antibodies with enhanced effector function

Also Published As

Publication number Publication date
CA2240609C (en) 2009-02-03
US6146894A (en) 2000-11-14
US7297837B1 (en) 2007-11-20
US20080313754A1 (en) 2008-12-18
US7829338B2 (en) 2010-11-09

Similar Documents

Publication Publication Date Title
CA2240609A1 (en) A method for generating hypermutable organisms
WO2001088192A3 (en) A method for generating hypermutable organisms
Jablonka et al. ‘Lamarckian’mechanisms in Darwinian evolution
NZ520338A (en) Method of producing an undifferentiated avian cell culture using avian primordial germ cells
EP0773288A3 (en) Chimeric animal and method for producing the same
HK1084417A1 (en) Progenitor cells from wharton's jelly of human umbilical cord
AU2001226023A1 (en) A method for generating hypermutable plants
AU6755096A (en) Mammalian artificial chromosomes
AU1595195A (en) Embryonic stem cells as nuclear donors and nuclear transfer techniques to produce chimeric and transgenic animals
IL110781A0 (en) Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
AU6118998A (en) Pluripotent cells having disrupted intrinsic genes
ATE501635T1 (en) MISMATCH REPAIR CHEMICAL INHIBITORS
DK0842289T3 (en) Homologous recombination in eukaryotic cells with inactivated mismatch repair
CA2179656A1 (en) Chromosomal expression of heterologous genes in bacterial cells
AU5314294A (en) Improvements in or relating to organic compounds
GB9918061D0 (en) Modified plants
HUP0001941A2 (en) Methods for production of hybrid wheat
AU6560200A (en) Method for culturing and/or treating cells
ATE360687T1 (en) HIGH EFFICIENCY METHODS AND COMPOSITIONS FOR INTEGRATING EXOGENEOUS DNA INTO GENOMIC SPERM DNA
EP2289308A3 (en) Breeding plants
WO1998051164A1 (en) Feed composition for broilers and method for breeding broilers
NO972277D0 (en) Process and cell for the production of variant nisin
AU2001216030A1 (en) A method for generating genetically altered antigens
EP1474522A4 (en) A method for generating engineered cells for locus specific gene regulation and analysis
CN1263704A (en) Method for breeding tetraploid fish

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20180813