CA2227342A1 - Screening methods for enzymes and enzyme kits - Google Patents

Screening methods for enzymes and enzyme kits

Info

Publication number
CA2227342A1
CA2227342A1 CA002227342A CA2227342A CA2227342A1 CA 2227342 A1 CA2227342 A1 CA 2227342A1 CA 002227342 A CA002227342 A CA 002227342A CA 2227342 A CA2227342 A CA 2227342A CA 2227342 A1 CA2227342 A1 CA 2227342A1
Authority
CA
Canada
Prior art keywords
dna
clones
screening
enzyme
library
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002227342A
Other languages
French (fr)
Other versions
CA2227342C (en
Inventor
Jay M. Short
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BP Corp North America Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/503,606 external-priority patent/US6004788A/en
Application filed by Individual filed Critical Individual
Publication of CA2227342A1 publication Critical patent/CA2227342A1/en
Application granted granted Critical
Publication of CA2227342C publication Critical patent/CA2227342C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions

Abstract

Recombinant enzyme libraries and kits where a plurality of enzymes are each characterized by different physical and/or chemical characteristics and classified by common characteristics. The characteristics are determined by screening of recombinant enzymes expressed by a DNA library produced from various microorganisms. Also disclosed is a process for identifying clones of a recombinant library which express a protein with a desired activity by screening a library of expression clones randomly produced from DNA of at least one microorganism, said screening being effected on expression products of said clones to thereby identify clones which express a protein with a desired activity. Also disclosed is a process of screening clones having DNA from an uncultivated microorganism for a specified protein activity by screening for a specified protein activity in a library of clones prepared by (i) recovering DNA from a DNA population derived from at least one uncultivated microorganism; and (ii) transforming a host with recovered DNA to produce a library of clones which is screened for the specified protein activity.

Claims (13)

1. An enzyme kit, comprising:
a package said package containing at least three different recombinant enzymes, each of said at least three different recombinant enzymes having at least two enzyme characteristics in common, one of said at least two common enzyme characteristics being a chemical characteristic and the other of said at least two common enzyme characteristics being a physical characteristic.
2. A process for obtaining a recombinant enzyme library, comprising:
screening recombinant proteins produced by a plurality of expression clones, each of which contains a polynucleotide derived from a microorganism, said screening being effected to determine the clones which produce recombinant enzymes and to determine a plurality of different enzyme characteristics for the recombinant enzymes. and classifying the recombinant enzymes by said enzyme characteristics.
3. The process of Claim 2 wherein said screening includes screening said recombinant enzymes for a chemical characteristic, and rescreening recombinant enzymes having said chemical characteristic for a second chemical characteristic.
4. A process for producing an enzyme screening kit. comprising:
placing in a package at least three different selected enzymes, said enzymes being selected from a recombinant enzyme library having a plurality of differentenzymes having different enzyme characteristics, said enzymes of said library being classified by said enzyme characteristics, each of said at least three different selected enzymes each having at least two common enzyme characteristics.
5. A process for identifying clones of a recombinant library which express a protein with a desired activity, comprising:
screening in the liquid phase a library of expression clones randomly produced from DNA of at least one microorganism, said screening being effected on expression products of said clones to thereby identify clones which express a protein with a desired activity.
6. The process of claim 5 wherein the DNA from the library of expression clones is produced is gene cluster DNA.
7. The process of claim 5 wherein said protein is an enzyme.
8. A process of screening clones having DNA from an uncultivated microorganism for a specified protein activity, which process comprises:
screening for a specified protein activity in a library of clones prepared by (i) recovering DNA from a DNA population derived from at least one uncultivated microorganism; and (ii) transforming a host with recovered DNA to produce a library of clones which is screened for the specified protein activity.
9. The process of claim 8 wherein the recovered DNA is amplified.
10. The process of claim 8 wherein the recovered DNA is ligated into a vector.
11. The process of claim 10 wherein the vector into which the recovered DNA is ligated comprises at least one DNA sequence capable of regulating production of a detectable enzyme activity from said recovered DNA.
12. The process of claim 8 wherein the vector into which the recovered DNA has been ligated is used to transform a host.
13. The process of claim 8 wherein the protein is an enzyme.
CA2227342A 1995-07-18 1996-07-17 Screening methods for enzymes and enzyme kits Expired - Lifetime CA2227342C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US08/503,606 1995-07-18
US08/503,606 US6004788A (en) 1995-07-18 1995-07-18 Enzyme kits and libraries
US56899495A 1995-12-07 1995-12-07
US08/568,994 1995-12-07
US08/657,409 US5958672A (en) 1995-07-18 1996-06-03 Protein activity screening of clones having DNA from uncultivated microorganisms
US08/657,904 1996-06-03
PCT/US1996/011854 WO1997004077A1 (en) 1995-07-18 1996-07-17 Screening methods for enzymes and enzyme kits

Publications (2)

Publication Number Publication Date
CA2227342A1 true CA2227342A1 (en) 1997-02-06
CA2227342C CA2227342C (en) 2011-03-29

Family

ID=27054566

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2227342A Expired - Lifetime CA2227342C (en) 1995-07-18 1996-07-17 Screening methods for enzymes and enzyme kits

Country Status (10)

Country Link
US (8) US5958672A (en)
EP (2) EP1696025B1 (en)
JP (1) JP2002514894A (en)
AT (2) ATE465246T1 (en)
AU (1) AU6547796A (en)
CA (1) CA2227342C (en)
DE (2) DE69638168D1 (en)
DK (2) DK0839185T3 (en)
ES (1) ES2277346T3 (en)
WO (1) WO1997004077A1 (en)

Families Citing this family (173)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6495348B1 (en) 1993-10-07 2002-12-17 Regents Of The University Of Minnesota Mitomycin biosynthetic gene cluster
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US20060257890A1 (en) * 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6168919B1 (en) * 1996-07-17 2001-01-02 Diversa Corporation Screening methods for enzymes and enzyme kits
US6057103A (en) * 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
US7018793B1 (en) * 1995-12-07 2006-03-28 Diversa Corporation Combinatorial screening of mixed populations of organisms
US6794127B1 (en) * 1997-06-16 2004-09-21 Diversa Corporation Capillary array-based sample screening
US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
DE69631787T2 (en) * 1995-12-07 2005-03-10 Diversa Corp., San Diego PROCESS FOR SCREENING ENZYMATIC ACTIVITY
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6096548A (en) * 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US6242211B1 (en) 1996-04-24 2001-06-05 Terragen Discovery, Inc. Methods for generating and screening novel metabolic pathways
US7883872B2 (en) * 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US5811381A (en) * 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
US20070009930A1 (en) * 1996-12-18 2007-01-11 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6399789B1 (en) * 1996-12-18 2002-06-04 Kosan Biosciences, Inc. Multi-plasmid method for preparing large libraries of polyketides and non-ribosomal peptides
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US7148054B2 (en) * 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
IL130635A0 (en) 1997-01-17 2000-06-01 Maxygen Inc Evolution of whole cells and organisms by recursive sequence recombination
US6159687A (en) * 1997-03-18 2000-12-12 Novo Nordisk A/S Methods for generating recombined polynucleotides
US6159688A (en) 1997-03-18 2000-12-12 Novo Nordisk A/S Methods of producing polynucleotide variants
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
US20030013115A1 (en) * 1997-06-16 2003-01-16 Diversa Corporation, A Delaware Corporation Capillary array-based sample screening
US20020015997A1 (en) * 1997-06-16 2002-02-07 Lafferty William Michael Capillary array-based sample screening
WO1999015897A1 (en) * 1997-09-19 1999-04-01 Chiron Corporation Subtractive protein screening for gene identification
US6833242B2 (en) * 1997-09-23 2004-12-21 California Institute Of Technology Methods for detecting and sorting polynucleotides based on size
US6846627B2 (en) * 1997-10-15 2005-01-25 Diversa Corporation Screening for novel compounds which regulate biological interactions
US6261842B1 (en) * 1997-10-23 2001-07-17 Wisconsin Alumni Research Foundation Microorganism genomics, compositions and methods related thereto
EP1030861A4 (en) 1997-10-31 2001-09-05 Maxygen Inc Modification of virus tropism and host range by viral genome shuffling
AU746786B2 (en) 1997-12-08 2002-05-02 California Institute Of Technology Method for creating polynucleotide and polypeptide sequences
US7390619B1 (en) 1998-02-11 2008-06-24 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
US6541011B2 (en) 1998-02-11 2003-04-01 Maxygen, Inc. Antigen library immunization
EP1073670A1 (en) 1998-05-01 2001-02-07 Maxygen, Inc. Optimization of pest resistance genes using dna shuffling
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
WO2000009727A2 (en) * 1998-08-12 2000-02-24 Maxygen, Inc. Dna shuffling to produce herbicide selective crops
US20060242731A1 (en) * 1998-08-12 2006-10-26 Venkiteswaran Subramanian DNA shuffling to produce herbicide selective crops
JP2002522072A (en) 1998-08-12 2002-07-23 マキシジェン， インコーポレイテッド DNA shuffling of monooxygenase gene for production of industrial chemicals.
ES2237159T3 (en) * 1998-10-06 2005-07-16 Mark Aaron Emalfarb TRANSFORMATION SYSTEM IN THE FIELD OF FILAMENT MICOTIC FUNGI IN CHRYSOSPORIUM.
JP2002526107A (en) 1998-10-07 2002-08-20 マキシジェン， インコーポレイテッド DNA shuffling to generate nucleic acids for mycotoxin detoxification
US6368793B1 (en) * 1998-10-14 2002-04-09 Microgenomics, Inc. Metabolic selection methods
WO2000028018A1 (en) 1998-11-10 2000-05-18 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimization of plant phenotypes
US6438561B1 (en) * 1998-11-19 2002-08-20 Navigation Technologies Corp. Method and system for using real-time traffic broadcasts with navigation systems
US20040005673A1 (en) * 2001-06-29 2004-01-08 Kevin Jarrell System for manipulating nucleic acids
CA2360011A1 (en) * 1999-01-05 2000-07-13 Trustees Of Boston University Ordered gene assembly
WO2000042559A1 (en) * 1999-01-18 2000-07-20 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US20030054390A1 (en) * 1999-01-19 2003-03-20 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6917882B2 (en) * 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
US20070065838A1 (en) * 1999-01-19 2007-03-22 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US7024312B1 (en) * 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
EP1108783A3 (en) 1999-01-19 2001-09-05 Maxygen, Inc. Oligonucleotide-mediated nucleic acid recombination
CA2361384A1 (en) 1999-02-11 2000-08-17 Sun Ai Raillard High throughput mass spectrometry
JP3399518B2 (en) * 1999-03-03 2003-04-21 インターナショナル・ビジネス・マシーンズ・コーポレーション Semiconductor structure and method of manufacturing the same
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
CA2362737A1 (en) 1999-03-05 2000-09-08 Maxygen, Inc. Recombination of insertion modified nucleic acids
WO2001004287A1 (en) * 1999-07-07 2001-01-18 Maxygen Aps A method for preparing modified polypeptides
US20040002474A1 (en) * 1999-10-07 2004-01-01 Maxygen Inc. IFN-alpha homologues
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US7115712B1 (en) * 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
US20040002147A1 (en) 1999-12-29 2004-01-01 Desantis Grace Nitrilases
WO2001051663A2 (en) * 2000-01-11 2001-07-19 Maxygen, Inc. Integrated systems and methods for diversity generation and screening
WO2001059071A2 (en) 2000-02-09 2001-08-16 Genvec, Inc. Methods of preparing and using a viral vector library
WO2001064864A2 (en) * 2000-02-28 2001-09-07 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
WO2001073000A2 (en) * 2000-03-24 2001-10-04 Maxygen, Inc. Methods for modulating cellular and organismal phenotypes
US7122330B2 (en) * 2000-04-13 2006-10-17 Mark Aaron Emalfarb High-throughput screening of expressed DNA libraries in filamentous fungi
US20010047029A1 (en) * 2000-04-26 2001-11-29 Handelsman Jo E. Triaryl cation antibiotics from environmental DNA
JP2003534806A (en) * 2000-05-31 2003-11-25 ジェンベク、インコーポレイティッド Methods and compositions for targeting adenovirus vectors
WO2002000897A2 (en) * 2000-06-23 2002-01-03 Maxygen, Inc. Novel chimeric promoters
AU2001272978A1 (en) * 2000-06-23 2002-01-08 Maxygen, Inc. Novel co-stimulatory molecules
AU2001273057A1 (en) * 2000-06-27 2002-01-08 Fluidigm Corporation A microfluidic design automation method and system
US20020072097A1 (en) * 2000-07-07 2002-06-13 Delcardayre Stephen Molecular breeding of transposable elements
US6858422B2 (en) * 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
US7435562B2 (en) 2000-07-21 2008-10-14 Modular Genetics, Inc. Modular vector systems
EP1373889A2 (en) * 2000-07-31 2004-01-02 Maxygen, Inc. Biosensors, reagents and diagnostic applications of directed evolution
AU2001280968A1 (en) * 2000-07-31 2002-02-13 Menzel, Rolf Compositions and methods for directed gene assembly
US6720168B2 (en) 2000-08-04 2004-04-13 Genencor International, Inc. 2,5-DKG permeases
BR0113032A (en) * 2000-08-04 2006-02-21 Genencor Int accentuation of industrial production increasing substrate transport
EP1317535A2 (en) * 2000-08-24 2003-06-11 Maxygen, Inc. Constructs and their use in metabolic pathway engineering
US20020086313A1 (en) * 2000-09-25 2002-07-04 Kilbane John J. Application of bioinformatics for direct study of unculturable microorganisms
JP2005501217A (en) * 2000-10-10 2005-01-13 ディベルサ コーポレーション High-throughput or capillary-based screening for bioactivity or biomolecules
US7202070B2 (en) * 2000-10-31 2007-04-10 Biocatalytics, Inc. Method for reductive amination of a ketone using a mutated enzyme
DE10062086A1 (en) * 2000-12-13 2002-07-04 Wella Ag Agent and method of dyeing keratin fibers
AU2002227883A1 (en) * 2001-01-25 2002-08-06 Evolva Biotech A/S A library of a collection of cells
US8008459B2 (en) * 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
EP1384022A4 (en) 2001-04-06 2004-08-04 California Inst Of Techn Nucleic acid amplification utilizing microfluidic devices
IL158419A0 (en) 2001-04-19 2004-05-12 Scripps Research Inst Methods and composition for the production of orthoganal trna-aminoacyl trna synthetase pairs
EP2327767B1 (en) 2001-06-21 2015-04-29 BASF Enzymes LLC Nitrilases
US7229811B2 (en) * 2001-08-03 2007-06-12 Genencor International, Inc. 2,5-diketo-D-gluconic acid (2,5-DKG) permeases
US20070298474A1 (en) * 2001-08-03 2007-12-27 Manoj Kumar Enhanced 2-Keto-L-Gulonic acid production
EP1578987A2 (en) * 2001-08-03 2005-09-28 Diversa Corporation Epoxide hydrolases, nucleic acids encoding them and methods for making and using them
GB0125043D0 (en) * 2001-10-17 2001-12-12 Biotica Tech Ltd Production, detection and use of transformant cells
AU2002366523A1 (en) * 2001-12-05 2003-06-23 E-Tenna Corporation Capacitively-loaded bent-wire monopole on an artificial magnetic conductor
DE10163748A1 (en) * 2001-12-21 2003-07-17 Henkel Kgaa New glycosyl hydrolases
JP4472345B2 (en) * 2002-01-25 2010-06-02 エヴォルヴァ・リミテッド Multi-parameter screening and evolution to multi-functional small molecule producing cells
MXPA04007886A (en) 2002-02-13 2004-10-15 Dow Global Technologies Inc Over-expression of extremozyme genes in pseudomonads and closely related bacteria.
WO2003072054A2 (en) * 2002-02-25 2003-09-04 Cabot Corporation Custom ligand design for biomolecular filtration and purification for bioseperation
WO2003085379A2 (en) 2002-04-01 2003-10-16 Fluidigm Corporation Microfluidic particle-analysis systems
US7312085B2 (en) * 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
BRPI0309391B1 (en) 2002-04-19 2018-10-30 Dsm Food Specialties B V vector comprising nucleic acid encoding phospholipase activity polypeptide, transformed cell, protein preparation, preparation method and related methods
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
BRPI0312121B1 (en) 2002-06-14 2019-10-01 Verenium Corporation PREPARATION OF PROTEIN, PROPERTY POLYPEPTIDE AND METHODS FOR PREPARING AND USING THEM
JP2005531001A (en) * 2002-06-24 2005-10-13 フルイディグム コーポレイション Recirculating fluid network and use thereof
WO2004016791A1 (en) * 2002-08-01 2004-02-26 Evolva Ltd Methods of mixing large numbers of heterologous genes
EP2275536A1 (en) 2002-08-06 2011-01-19 Verdia, Inc. AP1 amine oxidase variants
ATE471981T1 (en) * 2002-08-20 2010-07-15 B R A I N Biotechnology Res An ISOLATION AND CLONING OF DNA FROM UNCULTIVATED ORGANISMS
WO2004028955A2 (en) 2002-09-25 2004-04-08 California Institute Of Technology Microfluidic large scale integration
US8871446B2 (en) 2002-10-02 2014-10-28 California Institute Of Technology Microfluidic nucleic acid analysis
EP2062976B1 (en) 2002-10-16 2011-09-28 The Scripps Research Institute Site specific incorporation of keto amino acids into proteins
US8158768B2 (en) 2002-12-23 2012-04-17 Dynavax Technologies Corporation Immunostimulatory sequence oligonucleotides and methods of using the same
EP2194133B1 (en) 2003-03-06 2015-12-02 BASF Enzymes LLC Amylases, nucleic acids encoding them and methods for making and using them
MX295545B (en) 2003-03-07 2012-02-03 Diversa Corp Hydrolases, nucleic acids encoding them and mehods for making and using them.
MXPA05010681A (en) 2003-04-04 2005-12-15 Diversa Corp Pectate lyases, nucleic acids encoding them and methods for making and using them.
SG155063A1 (en) 2003-04-29 2009-09-30 Pioneer Hi Bred Int Novel glyphosate-n-acetyltransferase (gat) genes
US7960148B2 (en) 2003-07-02 2011-06-14 Verenium Corporation Glucanases, nucleic acids encoding them and methods for making and using them
WO2005012550A2 (en) * 2003-07-31 2005-02-10 Diversa Corporation Screening methods and libraries of trace amounts of dna from uncultivated microorganisms
WO2005021714A2 (en) 2003-08-11 2005-03-10 Diversa Corporation Laccases, nucleic acids encoding them and methods for making and using them
US20060094033A1 (en) * 2004-05-21 2006-05-04 Carl Abulencia Screening methods and libraries of trace amounts of DNA from uncultivated microorganisms
ES2461857T3 (en) 2004-06-16 2014-05-21 Dsm Ip Assets B.V. Compositions and methods for enzymatic discoloration of chlorophyll
US20050282155A1 (en) * 2004-06-17 2005-12-22 Lucigen Corporation Viral libraries from uncultivated viruses and polypeptides produced therefrom
US20080193912A1 (en) * 2004-08-03 2008-08-14 Yiu-Lian Fong Compositions and Methods for Preparation of Nucleic Acids from Microbial Samples
DE102004046116A1 (en) * 2004-09-23 2006-04-06 Henkel Kgaa Pullulanases from psychrophilic organisms
JP5135504B2 (en) 2004-10-27 2013-02-06 ザ スクリプス リサーチ インスティチュート Orthogonal translation components for in vivo incorporation of unnatural amino acids
ATE540110T1 (en) 2004-11-11 2012-01-15 Modular Genetics Inc OLIGONUCLEOTIDE LADDER CONSTRUCTION AND SYSTEM FOR GENERATING MOLECULAR DIVERSITY
US20090038023A1 (en) 2005-03-10 2009-02-05 Verenium Corporation Lyase Enzymes, Nucleic Acids Encoding Them and Methods For Making and Using Them
AU2006227965B2 (en) 2005-03-15 2013-01-31 Bp Corporation North America Inc. Cellulases, nucleic acids encoding them and methods for making and using them
MX348154B (en) 2005-06-21 2017-05-30 Xoma (Us) Llc IL-1ß BINDING ANTIBODIES AND FRAGMENTS THEREOF.
EP2216403A3 (en) 2006-02-02 2010-11-24 Verenium Corporation Esterases and related nucleic acids and methods
WO2007092475A2 (en) * 2006-02-06 2007-08-16 Franklin And Marshall College Site-specific incorporation of fluorinated amino acids into proteins
DK1989301T3 (en) 2006-02-10 2015-01-05 Bp Corp North America Inc Cellulolytic enzymes, nucleic acids encoding them, and methods for making and using them
CA2638801C (en) 2006-02-14 2016-12-13 Verenium Corporation Xylanases, nucleic acids encoding them and methods for making and using them
MX2008011477A (en) 2006-03-07 2008-09-23 Cargill Inc Aldolases, nucleic acids encoding them and methods for making and using them.
EA019971B1 (en) 2006-03-07 2014-07-30 Верениум Корпорейшн Aldolases, nucleic acids encoding them and methods for making and using them
WO2007136833A2 (en) * 2006-05-19 2007-11-29 Codon Devices, Inc. Methods and compositions for aptamer production and uses thereof
CN103397043A (en) 2006-08-04 2013-11-20 维莱尼姆公司 Glucanases, nucleic acids encoding same and method for making and using same
CA2663001A1 (en) 2006-09-21 2008-03-27 Verenium Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
DK2617814T3 (en) 2006-09-21 2016-02-22 Basf Enzymes Llc Phytases, nucleic acids encoding them, and methods of producing and using them
US8680252B2 (en) 2006-12-10 2014-03-25 Dyadic International (Usa), Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
US9862956B2 (en) 2006-12-10 2018-01-09 Danisco Us Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
EP2479267B1 (en) 2006-12-21 2016-12-21 BASF Enzymes LLC Amylases and glucoamylases, nucleic acids encoding them and methods for making and using them
AR065544A1 (en) 2007-01-30 2009-06-17 Verenium Corp ENZYMES FOR THE TREATMENT OF NUCLEIC ACID LIGNOCELLULOSICS THAT CODE AND METHODS TO PREPARE AND USE THEM
US9217164B2 (en) 2007-03-01 2015-12-22 Basf Enzymes Llc Nitrilases, nucleic acids encoding them and methods for making and using them
US8551751B2 (en) * 2007-09-07 2013-10-08 Dyadic International, Inc. BX11 enzymes having xylosidase activity
MX2010003600A (en) 2007-10-03 2010-12-14 Verenium Corp Xylanases, nucleic acids encoding them and methods for making and using them.
EP2238261B1 (en) 2008-01-03 2013-12-04 Verenium Corporation Isomerases, nucleic acids encoding them and methods for making and using them
US8709772B2 (en) 2008-01-03 2014-04-29 Verenium Corporation Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them
US8357503B2 (en) 2008-08-29 2013-01-22 Bunge Oils, Inc. Hydrolases, nucleic acids encoding them and methods for making and using them
US8198062B2 (en) 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
US8153391B2 (en) 2008-08-29 2012-04-10 Bunge Oils, Inc. Hydrolases, nucleic acids encoding them and methods for making and using them
MX357314B (en) 2008-09-26 2018-07-04 Ambrx Inc Non-natural amino acid replication-dependent microorganisms and vaccines.
NZ596459A (en) 2009-05-21 2013-11-29 Verenium Corp Phytases, nucleic acids encoding them and methods for making and using them
UA109884C2 (en) 2009-10-16 2015-10-26 A POLYPEPTIDE THAT HAS THE ACTIVITY OF THE PHOSPHATIDYLINOSYTOL-SPECIFIC PHOSPHOLIPASE C, NUCLEIC ACID, AND METHOD OF METHOD
UA111708C2 (en) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. METHOD OF OIL REFINING
US9187762B2 (en) 2010-08-13 2015-11-17 Pioneer Hi-Bred International, Inc. Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (HPPD) activity
WO2012106579A1 (en) 2011-02-03 2012-08-09 Bristol-Myers Squibb Company Amino acid dehydrogenase and its use in preparing amino acids from keto acids
US20130244292A1 (en) 2012-03-16 2013-09-19 Bp Corporation North America Inc. Polypeptides Having Endoglucanase Activity
CA2872096A1 (en) 2012-05-04 2013-11-07 E. I. Du Pont De Nemours And Company Compositions and methods comprising sequences having meganuclease activity
BR112015001344A2 (en) 2012-07-24 2017-07-04 Bp Corp North America Inc xylose isomerases and their uses
AU2014236162A1 (en) 2013-03-14 2015-09-17 Arzeda Corp. Compositions having dicamba decarboxylase activity and methods of use
US20140289906A1 (en) 2013-03-14 2014-09-25 Pioneer Hi-Bred International, Inc. Compositions Having Dicamba Decarboxylase Activity and Methods of Use
US10345289B2 (en) * 2013-04-18 2019-07-09 The Board Of Trustees Of The University Of Illinois Method and apparatus for analyzing a target material
US9580758B2 (en) 2013-11-12 2017-02-28 Luc Montagnier System and method for the detection and treatment of infection by a microbial agent associated with HIV infection
US11608516B2 (en) 2015-04-15 2023-03-21 Ecolab Usa Inc. Method for determination of diversity and viability thresholds used to assess microorganisms in process samples

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4675285A (en) * 1984-09-19 1987-06-23 Genetics Institute, Inc. Method for identification and isolation of DNA encoding a desired protein
JPH02238882A (en) * 1989-03-13 1990-09-21 Takara Shuzo Co Ltd Synthesis of cdna and kit therefor
WO1991016427A1 (en) * 1990-04-24 1991-10-31 Stratagene Methods for phenotype creation from multiple gene populations
US5352778A (en) * 1990-04-26 1994-10-04 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
AU651419B2 (en) 1990-12-18 1994-07-21 Elf France Bitumen/polymer blend giving bitumen/polymer compositions with very low heat sensitivity for use in surfacing
US5316935A (en) * 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
JPH11504218A (en) * 1995-04-24 1999-04-20 クロマゾーム コーポレーション Methods for generating and screening new metabolic pathways
US5654150A (en) * 1995-06-07 1997-08-05 President And Fellows Of Harvard College Method of expression cloning
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US6033883A (en) 1996-12-18 2000-03-07 Kosan Biosciences, Inc. Production of polyketides in bacteria and yeast
US6117659A (en) 1997-04-30 2000-09-12 Kosan Biosciences, Inc. Recombinant narbonolide polyketide synthase
US6090601A (en) 1998-01-23 2000-07-18 Kosan Bioscience Sorangium polyketide synthase
US6150513A (en) 1998-09-16 2000-11-21 Kosan Biosciences, Inc. Polyketide synthase enzymes and recombinant DNA constructs therefor
US6177262B1 (en) 1998-09-22 2001-01-23 Kosan Biosciences, Inc. Recombinant host cells for the production of polyketides

Also Published As

Publication number Publication date
CA2227342C (en) 2011-03-29
ATE465246T1 (en) 2010-05-15
US6677115B2 (en) 2004-01-13
DK0839185T3 (en) 2007-02-19
US20060068493A1 (en) 2006-03-30
EP0839185B1 (en) 2006-11-22
US20040029173A1 (en) 2004-02-12
EP0839185A1 (en) 1998-05-06
US20040005587A1 (en) 2004-01-08
DE69636721T2 (en) 2008-01-24
US6849395B2 (en) 2005-02-01
EP1696025B1 (en) 2010-04-21
EP1696025A2 (en) 2006-08-30
US20020086279A1 (en) 2002-07-04
JP2002514894A (en) 2002-05-21
WO1997004077A1 (en) 1997-02-06
AU6547796A (en) 1997-02-18
US6528249B1 (en) 2003-03-04
US20020172944A1 (en) 2002-11-21
DE69636721D1 (en) 2007-01-04
US6280926B1 (en) 2001-08-28
EP1696025A3 (en) 2006-09-13
DK1696025T3 (en) 2010-07-19
EP0839185A4 (en) 2002-10-30
ATE346148T1 (en) 2006-12-15
ES2277346T3 (en) 2007-07-01
DE69638168D1 (en) 2010-06-02
US5958672A (en) 1999-09-28

Similar Documents

Publication Publication Date Title
CA2227342A1 (en) Screening methods for enzymes and enzyme kits
Attardi Animal mitochondrial DNA: an extreme example of genetic economy
SG173213A1 (en) Identification of genes
AU4965499A (en) Method of using an improved peptide nucleic acid universal library to optimize dna sequence hybridation
EP0777750A4 (en) High throughput screening method for sequences or genetic alterations in nucleic acids
AU6414196A (en) A modified enzyme with lipolytic activity
DE69938722D1 (en) METHOD FOR THE PRODUCTION OF HETEROLOGIC POLYPEPTIDES IN TRICHOTHECENE LACK OF FILAMENT SOLVENTS MUSHROOMS
Takakura et al. NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae.
NO20010300D0 (en) New methods for identifying ligand and target biomolecules
AU3634697A (en) Systems for the mass production of proteins or peptides by microorganisms of the genus (humicola)
WO1998024906A3 (en) Isolated mammalian monocyte cell genes; related reagents
White et al. Genetic consequences of transfection with heteroduplex bacteriophage λ DNA
Clowes Investigation of the genetics of cysteineless mutants of Salmonella typhimurium by transduction
Alikhanian et al. Recombination in Actinomyces aureofaciens
CA2204743A1 (en) D-pantolactone hydrolase and gene encoding the same
Jenness et al. Genetic characterization of the folding domains of the catalytic chains in aspartate transcarbamoylase.
WO1997010366A3 (en) High throughput screening method for sequences or genetic alterations in nucleic acids
Makins et al. Intergeneric cosynthesis of penicillin by strains of Penicillium chrysogenum, P. chrysogenum/notatum and Aspergillus nidulans
Merrick Hybridization and selection for increased penicillin titre in wild-type isolates of Aspergillus nidulans
Leckie et al. A structural gene for Neurospora crassa isocitrate lyase
Dales et al. The location and analysis of two heterokaryon incompatibility (het) loci in strains of Aspergillus nidulans
Matur et al. The use of step enzymes as markers during meiosis and ascospore formation in Saccharomyces cerevisiae
WO2002029101A3 (en) Methods for cloning, sequencing and expressing genes from complex microbial populations
CN108588202A (en) A kind of unicellular full genome amplification method
Brammar Independent expression of the A gene of the tryptophan operon of Escherichia coli during tryptophan starvation

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20160718