CA2224674A1 - Immunoassay and kit with two reagents that are cross-linked if they adhere to an analyte - Google Patents

Immunoassay and kit with two reagents that are cross-linked if they adhere to an analyte

Info

Publication number
CA2224674A1
CA2224674A1 CA002224674A CA2224674A CA2224674A1 CA 2224674 A1 CA2224674 A1 CA 2224674A1 CA 002224674 A CA002224674 A CA 002224674A CA 2224674 A CA2224674 A CA 2224674A CA 2224674 A1 CA2224674 A1 CA 2224674A1
Authority
CA
Canada
Prior art keywords
cross
immunoassay
linked
kit
analyte
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002224674A
Other languages
French (fr)
Other versions
CA2224674C (en
Inventor
Ulf Landegren
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olink AB
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2224674A1 publication Critical patent/CA2224674A1/en
Application granted granted Critical
Publication of CA2224674C publication Critical patent/CA2224674C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Abstract

The present invention relates to an immunological test kit and immunoassay using a first immobilized antibody having affinity for a specific antigen. The invention is characterized by a second and third antibody being specific for different determinants of the antigen and modified with cross-linkable oligonucleotides. For detection, the oligonucleotides are amplified, whereby only such oligonucleotides will be amplified which have been cross-linked to each other. In this way unspecific background is avoided and detection is possible down to single molecules.
CA002224674A 1995-06-16 1996-06-14 Immunoassay and kit with two reagents that are cross-linked if they adhere to an analyte Expired - Lifetime CA2224674C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE9502196-0 1995-06-16
SE9502196A SE504798C2 (en) 1995-06-16 1995-06-16 Immunoassay and test kits with two reagents that can be cross-linked if adsorbed to the analyte
PCT/SE1996/000779 WO1997000446A1 (en) 1995-06-16 1996-06-14 Immunoassay and kit with two reagents that are cross-linked if they adhere to an analyte

Publications (2)

Publication Number Publication Date
CA2224674A1 true CA2224674A1 (en) 1997-01-03
CA2224674C CA2224674C (en) 2009-02-24

Family

ID=20398643

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002224674A Expired - Lifetime CA2224674C (en) 1995-06-16 1996-06-14 Immunoassay and kit with two reagents that are cross-linked if they adhere to an analyte

Country Status (8)

Country Link
US (2) US6878515B1 (en)
EP (1) EP0832431B1 (en)
JP (1) JP4091113B2 (en)
AU (1) AU702125B2 (en)
CA (1) CA2224674C (en)
DE (1) DE69614539T2 (en)
SE (1) SE504798C2 (en)
WO (1) WO1997000446A1 (en)

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Also Published As

Publication number Publication date
DE69614539D1 (en) 2001-09-20
EP0832431A1 (en) 1998-04-01
SE9502196D0 (en) 1995-06-16
JPH11508040A (en) 1999-07-13
AU6143996A (en) 1997-01-15
US20050233351A1 (en) 2005-10-20
AU702125B2 (en) 1999-02-11
JP4091113B2 (en) 2008-05-28
SE9502196L (en) 1996-12-17
WO1997000446A1 (en) 1997-01-03
SE504798C2 (en) 1997-04-28
CA2224674C (en) 2009-02-24
EP0832431B1 (en) 2001-08-16
US6878515B1 (en) 2005-04-12
DE69614539T2 (en) 2002-05-23

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