CA2205006A1 - Modified green fluorescent proteins - Google Patents

Modified green fluorescent proteins

Info

Publication number
CA2205006A1
CA2205006A1 CA002205006A CA2205006A CA2205006A1 CA 2205006 A1 CA2205006 A1 CA 2205006A1 CA 002205006 A CA002205006 A CA 002205006A CA 2205006 A CA2205006 A CA 2205006A CA 2205006 A1 CA2205006 A1 CA 2205006A1
Authority
CA
Canada
Prior art keywords
wild
gfp
product derived
modifications
modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002205006A
Other languages
French (fr)
Other versions
CA2205006C (en
Inventor
Roger Y. Tsien
Roger Heim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
Original Assignee
Roger Y. Tsien
Roger Heim
The Regents Of The University Of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23322562&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2205006(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Roger Y. Tsien, Roger Heim, The Regents Of The University Of California filed Critical Roger Y. Tsien
Priority to CA002343586A priority Critical patent/CA2343586C/en
Publication of CA2205006A1 publication Critical patent/CA2205006A1/en
Application granted granted Critical
Publication of CA2205006C publication Critical patent/CA2205006C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/43504—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates
    • G01N2333/43595—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/855—Proteins from animals other than mammals or birds

Abstract

Modifications in the sequence of Aequorea wild-type GFP provide products having markedly different excitation and emission spectra from corresponding products from wild-type GFP. In one class of modifications, the product derived from the modified GFP exhibits an alteration in the ratio of two main excitation peaks observed with the product derived from wild-type GFP. In another class, the product derived from the modified GFP fluoresces at a shorter wavelength than the corresponding product from wild-type GFP. In yet another class of modifications, the product derived from the modified GFP
exhibits only a single excitation peak and enhanced emission relative to the product derived from wild-type GFP.
CA002205006A 1994-11-10 1995-11-13 Modified green fluorescent proteins Expired - Lifetime CA2205006C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002343586A CA2343586C (en) 1994-11-10 1995-11-13 Modified green fluorescent proteins

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/337,915 US5625048A (en) 1994-11-10 1994-11-10 Modified green fluorescent proteins
US08/337,915 1994-11-10
PCT/US1995/014692 WO1996023810A1 (en) 1994-11-10 1995-11-13 Modified green fluorescenct proteins

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002343586A Division CA2343586C (en) 1994-11-10 1995-11-13 Modified green fluorescent proteins

Publications (2)

Publication Number Publication Date
CA2205006A1 true CA2205006A1 (en) 1996-08-08
CA2205006C CA2205006C (en) 2001-07-24

Family

ID=23322562

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002205006A Expired - Lifetime CA2205006C (en) 1994-11-10 1995-11-13 Modified green fluorescent proteins

Country Status (12)

Country Link
US (4) US5625048A (en)
EP (2) EP1104769B1 (en)
JP (1) JP3283523B2 (en)
AT (1) ATE201878T1 (en)
AU (1) AU702205B2 (en)
CA (1) CA2205006C (en)
DE (1) DE69521219T2 (en)
DK (2) DK0804457T3 (en)
ES (1) ES2159650T3 (en)
GR (1) GR3036264T3 (en)
PT (1) PT804457E (en)
WO (1) WO1996023810A1 (en)

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