CA2186021A1 - Purified dna polymerase from bacillus stearothermophilus - Google Patents
Purified dna polymerase from bacillus stearothermophilusInfo
- Publication number
- CA2186021A1 CA2186021A1 CA002186021A CA2186021A CA2186021A1 CA 2186021 A1 CA2186021 A1 CA 2186021A1 CA 002186021 A CA002186021 A CA 002186021A CA 2186021 A CA2186021 A CA 2186021A CA 2186021 A1 CA2186021 A1 CA 2186021A1
- Authority
- CA
- Canada
- Prior art keywords
- dna polymerase
- bacillus stearothermophilus
- purified dna
- methods
- enzymes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Abstract
Composition and methods for the expression of recombinant DNA polymerase enzymes derived from Bacillus stearothermophilus.
The present invention also concerns methods for purifying recombinant Bst DNA polymerase enzymes, compositions containing the purified enzymes in a form suitable for conducting biochemical reactions, and methods for using the purified enzymes.
The present invention also concerns methods for purifying recombinant Bst DNA polymerase enzymes, compositions containing the purified enzymes in a form suitable for conducting biochemical reactions, and methods for using the purified enzymes.
Applications Claiming Priority (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US22261294A | 1994-04-01 | 1994-04-01 | |
US30741094A | 1994-09-16 | 1994-09-16 | |
US08/394,232 | 1995-02-24 | ||
US08/307,410 | 1995-02-24 | ||
US08/394,232 US6100078A (en) | 1994-04-01 | 1995-02-24 | Purified DNA polymerase from bacillus stearothermophilus ATCC 12980 |
US08/222,612 | 1995-02-24 | ||
PCT/US1995/004080 WO1995027067A1 (en) | 1994-04-01 | 1995-03-30 | Purified dna polymerase from bacillus stearothermophilus |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2186021A1 true CA2186021A1 (en) | 1995-10-12 |
CA2186021C CA2186021C (en) | 2002-07-09 |
Family
ID=27397117
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002186021A Expired - Fee Related CA2186021C (en) | 1994-04-01 | 1995-03-30 | Purified dna polymerase from bacillus stearothermophilus |
Country Status (6)
Country | Link |
---|---|
US (3) | US6100078A (en) |
EP (1) | EP0699760A1 (en) |
JP (1) | JPH09511643A (en) |
AU (1) | AU694952B2 (en) |
CA (1) | CA2186021C (en) |
WO (1) | WO1995027067A1 (en) |
Families Citing this family (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5814506A (en) * | 1995-08-02 | 1998-09-29 | New England Biolabs, Inc. | Over-expression and purification of a truncated thermostable DNA polymerase by protein fusion |
CA2210506C (en) * | 1996-09-23 | 2001-04-17 | Becton, Dickinson And Company | Thermostable dna polymerase from bacillus pallidus |
US7556958B1 (en) * | 1997-04-08 | 2009-07-07 | The Rockefeller University | Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof |
SG86311A1 (en) * | 1997-04-10 | 2002-02-19 | Univ Singapore | Bacillus stearothermophilus dna polymerase i (klenow) clones with reduced 3'-to-5' exonuclease activity |
EP0921196A1 (en) | 1997-12-02 | 1999-06-09 | Roche Diagnostics GmbH | Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction |
US6703211B1 (en) | 1998-03-13 | 2004-03-09 | Promega Corporation | Cellular detection by providing high energy phosphate donor other than ADP to produce ATP |
US6268146B1 (en) | 1998-03-13 | 2001-07-31 | Promega Corporation | Analytical methods and materials for nucleic acid detection |
US6391551B1 (en) | 1998-03-13 | 2002-05-21 | Promega Corporation | Detection of nucleic acid hybrids |
US6235480B1 (en) | 1998-03-13 | 2001-05-22 | Promega Corporation | Detection of nucleic acid hybrids |
US6335162B1 (en) | 1998-03-13 | 2002-01-01 | Promega Corporation | Nucleic acid detection |
US6270974B1 (en) | 1998-03-13 | 2001-08-07 | Promega Corporation | Exogenous nucleic acid detection |
US7090975B2 (en) | 1998-03-13 | 2006-08-15 | Promega Corporation | Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection |
US6312902B1 (en) | 1998-03-13 | 2001-11-06 | Promega Corporation | Nucleic acid detection |
US6270973B1 (en) | 1998-03-13 | 2001-08-07 | Promega Corporation | Multiplex method for nucleic acid detection |
CA2374494A1 (en) * | 1999-05-22 | 2000-11-30 | Epicentre Technologies Corporation | Reverse transcription activity from bacillus stearothermophilus dna polymerase in the presence of magnesium |
US6436677B1 (en) * | 2000-03-02 | 2002-08-20 | Promega Corporation | Method of reverse transcription |
US6632645B1 (en) * | 2000-03-02 | 2003-10-14 | Promega Corporation | Thermophilic DNA polymerases from Thermoactinomyces vulgaris |
WO2002101004A2 (en) * | 2001-06-08 | 2002-12-19 | Shanghai Mendel Dna Center Co., Ltd | Low-temperature cycle extension of dna with high priming specificity |
CA2460546A1 (en) * | 2001-09-14 | 2003-03-27 | Invitrogen Corporation | Dna polymerases and mutants thereof |
US20030180741A1 (en) | 2001-12-21 | 2003-09-25 | Holly Hogrefe | High fidelity DNA polymerase compositions and uses therefor |
US7148049B2 (en) | 2002-04-02 | 2006-12-12 | Roche Molecular Systems, Inc. | Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity |
WO2005030995A1 (en) * | 2003-09-23 | 2005-04-07 | University Of Missouri | Methods of synthesizing polynucleotides using thermostable enzymes |
US7179206B2 (en) * | 2004-03-11 | 2007-02-20 | Home Stretch Products, Inc. | Non-weight bearing foot and leg exercising apparatus |
US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
CA2621146A1 (en) * | 2005-09-06 | 2007-03-15 | Gen-Probe Incorporated | Methods, compositions and kits for isothermal amplification of nucleic acids |
JP2009048280A (en) * | 2007-08-15 | 2009-03-05 | Nikon Corp | Mechanism analysis program |
ATE497022T1 (en) * | 2007-08-15 | 2011-02-15 | Fujifilm Corp | METHOD FOR AMPLIFICATION OF NUCLEIC ACID |
US9868945B2 (en) * | 2011-02-08 | 2018-01-16 | Life Technologies Corporation | Linking methods, compositions, systems, kits and apparatuses |
WO2014158628A1 (en) | 2013-03-14 | 2014-10-02 | Hologic, Inc. | Compositions and methods for analysis of nucleic acid molecules |
EP3841223A1 (en) | 2018-08-08 | 2021-06-30 | Gen-Probe Incorporated | Compositions, methods and kits for detecting mycoplasma genitalium |
AU2020335996A1 (en) | 2019-08-23 | 2022-03-31 | Gen-Probe Incorporated | Compositions, methods and kits for detecting Treponema pallidum |
CA3153514A1 (en) | 2019-09-05 | 2021-03-11 | Gen-Probe Incorporated | Detection of chlamydia trachomatis nucleic acid variants |
CN117187210B (en) * | 2023-11-02 | 2024-01-23 | 广州达安基因股份有限公司 | Mutant Bst DNA polymerase large fragment and preparation method thereof |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SU1311251A1 (en) * | 1985-07-04 | 1988-01-30 | Ленинградский Институт Ядерной Физики Им.Б.П.Константинова | Strain bacillus starothermophilus b-3361 - producer of thermophil dna-polymerase and method of producing thermophil dna-polymerase |
US4889818A (en) * | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
US5079352A (en) * | 1986-08-22 | 1992-01-07 | Cetus Corporation | Purified thermostable enzyme |
US5466591A (en) * | 1986-08-22 | 1995-11-14 | Hoffmann-La Roche Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
US5322785A (en) * | 1990-04-26 | 1994-06-21 | New England Biolabs, Inc. | Purified thermostable DNA polymerase obtainable from thermococcus litoralis |
DK0550696T3 (en) * | 1990-09-28 | 2001-03-19 | Hoffmann La Roche | Purified thermostable nucleic acid polymerase enzyme from Thermosipho africanus |
AU8906091A (en) * | 1990-10-05 | 1992-04-28 | Wayne M. Barnes | Thermostable dna polymerase |
US6083686A (en) * | 1990-10-26 | 2000-07-04 | Johnson & Johnson Clinical Diagnostic Systems, Inc. | Increased production of Thermus aquaticus DNA polymerase in E. coli |
US5455166A (en) * | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
EP1233061A3 (en) * | 1991-06-03 | 2002-09-04 | Takara Shuzo Co., Ltd. | A method for cloning of a gene for pol I type DNA polymerase |
DE547359T1 (en) * | 1991-12-18 | 1993-11-25 | New England Biolabs Inc | Purified thermostable DNA polymerase from Pyrococcus species. |
DE547920T1 (en) * | 1991-12-18 | 1994-02-03 | New England Biolabs Inc | Archaebacteria recombinant thermostable DNA polymerase. |
JP3046142B2 (en) * | 1992-04-27 | 2000-05-29 | 寳酒造株式会社 | DNA polymerase gene |
WO1994016107A1 (en) * | 1993-01-12 | 1994-07-21 | Thomas Jefferson University | DNA SEQUENCING WITH Bst POLYMERASE |
KR100189229B1 (en) * | 1993-07-23 | 1999-06-01 | 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 | Methods for enhancing nucleic acid amplification |
-
1995
- 1995-02-24 US US08/394,232 patent/US6100078A/en not_active Expired - Lifetime
- 1995-03-30 WO PCT/US1995/004080 patent/WO1995027067A1/en active Application Filing
- 1995-03-30 AU AU22771/95A patent/AU694952B2/en not_active Ceased
- 1995-03-30 JP JP7525895A patent/JPH09511643A/en active Pending
- 1995-03-30 CA CA002186021A patent/CA2186021C/en not_active Expired - Fee Related
- 1995-04-03 EP EP95104961A patent/EP0699760A1/en not_active Withdrawn
- 1995-05-08 US US08/436,664 patent/US5874282A/en not_active Expired - Fee Related
-
1998
- 1998-08-18 US US09/135,642 patent/US6066483A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US6100078A (en) | 2000-08-08 |
CA2186021C (en) | 2002-07-09 |
US6066483A (en) | 2000-05-23 |
AU2277195A (en) | 1995-10-23 |
WO1995027067A1 (en) | 1995-10-12 |
EP0699760A1 (en) | 1996-03-06 |
AU694952B2 (en) | 1998-08-06 |
US5874282A (en) | 1999-02-23 |
JPH09511643A (en) | 1997-11-25 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |