CA2156066A1 - An enzyme feed additive and animal feed including it - Google Patents

An enzyme feed additive and animal feed including it

Info

Publication number
CA2156066A1
CA2156066A1 CA002156066A CA2156066A CA2156066A1 CA 2156066 A1 CA2156066 A1 CA 2156066A1 CA 002156066 A CA002156066 A CA 002156066A CA 2156066 A CA2156066 A CA 2156066A CA 2156066 A1 CA2156066 A1 CA 2156066A1
Authority
CA
Canada
Prior art keywords
feed
additive
enzyme
endoglucanases
cellobiohydrolase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002156066A
Other languages
French (fr)
Other versions
CA2156066C (en
Inventor
Michael Richard Bedford
Andrew John Morgan
Timothy Fowler
Kathleen A. Clarkson
Michael Ward
Katherine D. Collier
Edmund Larenas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco UK Ltd
Danisco US Inc
Original Assignee
Michael Richard Bedford
Andrew John Morgan
Timothy Fowler
Kathleen A. Clarkson
Michael Ward
Katherine D. Collier
Edmund Larenas
Finnfeeds International Limited
Genencor International, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22617873&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2156066(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Michael Richard Bedford, Andrew John Morgan, Timothy Fowler, Kathleen A. Clarkson, Michael Ward, Katherine D. Collier, Edmund Larenas, Finnfeeds International Limited, Genencor International, Inc. filed Critical Michael Richard Bedford
Publication of CA2156066A1 publication Critical patent/CA2156066A1/en
Application granted granted Critical
Publication of CA2156066C publication Critical patent/CA2156066C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

Abstract

The use is provided of a composition as a feed additive which comprises one or more endoglucanases, and 0-20 % by weight, based upon the content of cellulase proteins in the composition, of a cellobiohydrolase. The endoglycanases may be one or more of EGI, EGII, EGIII and any functionally active derivative of any thereof. Such endoglucanases may be obtained from a genetically modified strain of the fungus Trichoderma. Also provided is an enzyme-based feed additive which comprises EGI and/or EGII which lack the cellulose binding domain, and 0-20 % by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. A further enzyme-based feed additive is provided which comprises a cereal-based carrier, one or more endoglucanases, and 0-20 % by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. Such enzyme-based feed additives can be incorporated into a cereal-based feed which includes one or more of barley, wheat, triticale, rye and maize. The feed additive has the advantage of improving the feed conversion ratio and/or increasing the digestibility of a cereal-based feed in which it is included.
CA002156066A 1993-12-17 1994-12-19 An enzyme feed additive and animal feed including it Expired - Lifetime CA2156066C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/169,948 US5861271A (en) 1993-12-17 1993-12-17 Cellulase enzymes and systems for their expressions
US08/169,948 1993-12-17
PCT/EP1994/004212 WO1995016360A1 (en) 1993-12-17 1994-12-19 An enzyme feed additive and animal feed including it

Publications (2)

Publication Number Publication Date
CA2156066A1 true CA2156066A1 (en) 1995-06-22
CA2156066C CA2156066C (en) 2005-07-12

Family

ID=22617873

Family Applications (2)

Application Number Title Priority Date Filing Date
CA002156066A Expired - Lifetime CA2156066C (en) 1993-12-17 1994-12-19 An enzyme feed additive and animal feed including it
CA002178636A Abandoned CA2178636A1 (en) 1993-12-17 1994-12-19 Novel cellulase enzymes and systems for their expression

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA002178636A Abandoned CA2178636A1 (en) 1993-12-17 1994-12-19 Novel cellulase enzymes and systems for their expression

Country Status (16)

Country Link
US (3) US5861271A (en)
EP (3) EP1700524B1 (en)
JP (1) JPH09506514A (en)
CN (1) CN1093379C (en)
AT (3) ATE203373T1 (en)
AU (1) AU685210B2 (en)
CA (2) CA2156066C (en)
DE (3) DE69427812T2 (en)
DK (2) DK0684770T3 (en)
ES (1) ES2160694T3 (en)
FI (2) FI116965B (en)
HK (1) HK1097163A1 (en)
NO (1) NO314562B1 (en)
NZ (1) NZ278373A (en)
PT (1) PT684770E (en)
WO (2) WO1995016360A1 (en)

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