CA2139670A1 - Process for immobilizing enzymes to the cell wall of a microbial cell by producing a fusion protein - Google Patents
Process for immobilizing enzymes to the cell wall of a microbial cell by producing a fusion proteinInfo
- Publication number
- CA2139670A1 CA2139670A1 CA002139670A CA2139670A CA2139670A1 CA 2139670 A1 CA2139670 A1 CA 2139670A1 CA 002139670 A CA002139670 A CA 002139670A CA 2139670 A CA2139670 A CA 2139670A CA 2139670 A1 CA2139670 A1 CA 2139670A1
- Authority
- CA
- Canada
- Prior art keywords
- cell wall
- enzyme
- cell
- protein
- producing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
- C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/16—Enzymes or microbial cells immobilised on or in a biological cell
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
Abstract
A method is provided for immobilizing an enzyme, comprising immobilizing the enzyme or a functional part thereof to the cell wall of a microbial cell using recombinant DNA techniques. The enzyme is immobilized by linking it to the C-terminal part of a protein that ensures anchoring in the cell wall. Also provided is a recombinant polynucleotide comprising a structural gene encoding an enzyme protein, a part of a gene encoding the C-terminal part of a protein capable of anchoring in a eukaryotic or prokariotic cell wall, as well as a signal sequence, in addition to a chimeric protein encoded by the recombinant polynucleotide and a vector and a microorganism containing the polynucleotide. The microorganism is suitable for carrying out enzymatic processes on an industrial scale.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP92202080.5 | 1992-07-08 | ||
EP92202080 | 1992-07-08 | ||
EP92203899 | 1992-12-14 | ||
EP92203899.7 | 1992-12-14 | ||
PCT/EP1993/001763 WO1994001567A1 (en) | 1992-07-08 | 1993-07-07 | Process for immobilizing enzymes to the cell wall of a microbial cell by producing a fusion protein |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2139670A1 true CA2139670A1 (en) | 1994-01-20 |
CA2139670C CA2139670C (en) | 2005-09-27 |
Family
ID=26131543
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002139670A Expired - Lifetime CA2139670C (en) | 1992-07-08 | 1993-07-07 | Process for immobilizing enzymes to the cell wall of a microbial cell by producing a fusion protein |
Country Status (16)
Country | Link |
---|---|
US (1) | US6027910A (en) |
EP (1) | EP0673427B1 (en) |
JP (2) | JP3681385B2 (en) |
KR (1) | KR950702634A (en) |
AT (1) | ATE236257T1 (en) |
AU (1) | AU685057B2 (en) |
CA (1) | CA2139670C (en) |
DE (1) | DE69332829T2 (en) |
DK (1) | DK0673427T3 (en) |
ES (1) | ES2196009T3 (en) |
FI (1) | FI950042A (en) |
NZ (1) | NZ254108A (en) |
OA (1) | OA10123A (en) |
PT (1) | PT673427E (en) |
RU (1) | RU95105899A (en) |
WO (1) | WO1994001567A1 (en) |
Families Citing this family (39)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5866344A (en) * | 1991-11-15 | 1999-02-02 | Board Of Regents, The University Of Texas System | Antibody selection methods using cell surface expressed libraries |
US20030036092A1 (en) * | 1991-11-15 | 2003-02-20 | Board Of Regents, The University Of Texas System | Directed evolution of enzymes and antibodies |
ZA94916B (en) * | 1993-02-10 | 1995-08-10 | Unilever Plc | Immobilized proteins with specific binding capacities and their use in processes and products |
KR100227405B1 (en) * | 1993-02-26 | 1999-12-01 | . | Yeast agglutination genes and yeast containing them |
GB9423367D0 (en) * | 1994-11-18 | 1995-01-11 | Wellcome Found | Enzyme prodrug therapy |
US6696251B1 (en) | 1996-05-31 | 2004-02-24 | Board Of Trustees Of The University Of Illinois | Yeast cell surface display of proteins and uses thereof |
US6300065B1 (en) | 1996-05-31 | 2001-10-09 | Board Of Trustees Of The University Of Illinois | Yeast cell surface display of proteins and uses thereof |
US6699658B1 (en) | 1996-05-31 | 2004-03-02 | Board Of Trustees Of The University Of Illinois | Yeast cell surface display of proteins and uses thereof |
US6759243B2 (en) | 1998-01-20 | 2004-07-06 | Board Of Trustees Of The University Of Illinois | High affinity TCR proteins and methods |
WO2000058342A1 (en) | 1999-03-25 | 2000-10-05 | Valtion Teknillinen Tutkimuskeskus | Process for partitioning of proteins |
JP2003284556A (en) * | 1999-11-19 | 2003-10-07 | Toyota Motor Corp | Gene expressing protein capable of capturing metal |
ATE428733T1 (en) | 2000-03-14 | 2009-05-15 | Unilever Nv | VARIABLE DOMAIN OF THE HEAVY CHAIN OF AN ANTIBODY TO HUMAN NUTRITIONAL LIPASES AND THEIR USES |
DE10016279A1 (en) * | 2000-04-03 | 2001-10-04 | Henkel Kgaa | Enzymatic stain |
EP1283261B1 (en) * | 2000-05-01 | 2008-08-06 | Daiichi Sankyo Company, Limited | Method of screening drug acting on cell wall |
US7063738B2 (en) * | 2000-05-15 | 2006-06-20 | Schlumberger Technology Corporation | Permeable cements |
BR0112263A (en) | 2000-07-07 | 2003-06-24 | Eisai Co Ltd | Fungus Cell Wall Synthesis Gene |
AU2002220275A1 (en) * | 2000-12-08 | 2002-06-18 | The Board Of Trustees Of The University Of Illinois | Mutated class ii major histocompatibility proteins |
DK1380592T3 (en) * | 2001-04-19 | 2009-01-26 | Kansai Chem Eng | Cell surface layer binding protein and its use |
DE60232071D1 (en) | 2001-06-11 | 2009-06-04 | Applied Nanosystems Bv | METHOD OF BINDING ACMA-TYPE PROTEIN ANCHOR FUSIONS ON MICRO-ORGANISMS OF CELL WALL MATERIALS |
ES2327905T3 (en) | 2001-10-01 | 2009-11-05 | Dyax Corp. | MULTI-CHAIN EUCARIOT PRESENTATION VECTORS AND USES OF THE SAME. |
CA2464396A1 (en) | 2001-10-22 | 2003-05-01 | Daiichi Pharmaceutical Co., Ltd. | Method for screening agent acting on cell wall |
US20040067532A1 (en) | 2002-08-12 | 2004-04-08 | Genetastix Corporation | High throughput generation and affinity maturation of humanized antibody |
US7563600B2 (en) | 2002-09-12 | 2009-07-21 | Combimatrix Corporation | Microarray synthesis and assembly of gene-length polynucleotides |
EP1689348B1 (en) | 2003-12-05 | 2013-05-15 | Children's Hospital Medical Center | Oligosaccharide compositions and use thereof in the treatment of infection |
CA2614181A1 (en) * | 2005-07-01 | 2007-01-11 | Medimmune, Inc. | An integrated approach for generating multidomain protein therapeutics |
AU2006267039A1 (en) * | 2005-07-12 | 2007-01-18 | Codon Devices, Inc. | Compositions and methods for biocatalytic engineering |
WO2007047578A2 (en) | 2005-10-14 | 2007-04-26 | Medimmune, Inc. | Cell display of antibody libraries |
US20070231805A1 (en) * | 2006-03-31 | 2007-10-04 | Baynes Brian M | Nucleic acid assembly optimization using clamped mismatch binding proteins |
US20080138663A1 (en) * | 2006-12-12 | 2008-06-12 | Canon Kabushiki Kaisha | Microbial electrode and fuel cell and sensor using the same |
US20110076752A1 (en) * | 2007-02-09 | 2011-03-31 | Medimmune, Llc | Antibody library display by yeast cell plasma membrane |
EP2617827A1 (en) | 2007-03-26 | 2013-07-24 | Celexion, LLC | Method for displaying engineered proteins on a cell surface |
US8877688B2 (en) * | 2007-09-14 | 2014-11-04 | Adimab, Llc | Rationally designed, synthetic antibody libraries and uses therefor |
DK3124497T3 (en) | 2007-09-14 | 2020-05-11 | Adimab Llc | RATIONALE DESIGNED SYNTHETIC ANTIBODY LIBRARIES AND APPLICATIONS THEREOF |
EA201100943A1 (en) | 2008-12-16 | 2012-05-30 | Новартис Аг | YEAST DISPLAY SYSTEMS |
JP6266343B2 (en) | 2010-07-16 | 2018-01-24 | アディマブ, エルエルシー | Antibody library |
US8759044B2 (en) | 2011-03-23 | 2014-06-24 | Butamax Advanced Biofuels Llc | In situ expression of lipase for enzymatic production of alcohol esters during fermentation |
US8765425B2 (en) | 2011-03-23 | 2014-07-01 | Butamax Advanced Biofuels Llc | In situ expression of lipase for enzymatic production of alcohol esters during fermentation |
WO2022162043A1 (en) | 2021-01-28 | 2022-08-04 | Novozymes A/S | Lipase with low malodor generation |
WO2023203080A1 (en) | 2022-04-20 | 2023-10-26 | Novozymes A/S | Process for producing free fatty acids |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0398944A4 (en) * | 1988-02-05 | 1991-09-11 | Commonwealth Scientific And Industrial Research Organisation | Gene expression system (particularly for rotavirus vp7 protein) involving a foreign signal peptide and optionally a transmembrane anchor sequence |
CA2074089C (en) * | 1990-01-23 | 2001-04-10 | Tse-Wen Chang | Extracellular segments of human e immunoglobulin anchoring peptides and antibodies specific therefor |
US5348867A (en) * | 1991-11-15 | 1994-09-20 | George Georgiou | Expression of proteins on bacterial surface |
-
1993
- 1993-07-07 PT PT93915827T patent/PT673427E/en unknown
- 1993-07-07 DE DE69332829T patent/DE69332829T2/en not_active Expired - Lifetime
- 1993-07-07 JP JP50295294A patent/JP3681385B2/en not_active Expired - Lifetime
- 1993-07-07 DK DK93915827T patent/DK0673427T3/en active
- 1993-07-07 AU AU45653/93A patent/AU685057B2/en not_active Expired
- 1993-07-07 KR KR1019950700062A patent/KR950702634A/en not_active Application Discontinuation
- 1993-07-07 ES ES93915827T patent/ES2196009T3/en not_active Expired - Lifetime
- 1993-07-07 AT AT93915827T patent/ATE236257T1/en active
- 1993-07-07 CA CA002139670A patent/CA2139670C/en not_active Expired - Lifetime
- 1993-07-07 US US08/362,525 patent/US6027910A/en not_active Expired - Lifetime
- 1993-07-07 NZ NZ254108A patent/NZ254108A/en unknown
- 1993-07-07 WO PCT/EP1993/001763 patent/WO1994001567A1/en active IP Right Grant
- 1993-07-07 EP EP93915827A patent/EP0673427B1/en not_active Expired - Lifetime
- 1993-07-07 RU RU95105899/13A patent/RU95105899A/en unknown
-
1995
- 1995-01-04 FI FI950042A patent/FI950042A/en unknown
- 1995-01-06 OA OA60600A patent/OA10123A/en unknown
-
2004
- 2004-04-12 JP JP2004117071A patent/JP2004254700A/en active Pending
Also Published As
Publication number | Publication date |
---|---|
AU685057B2 (en) | 1998-01-15 |
DK0673427T3 (en) | 2003-07-21 |
JP3681385B2 (en) | 2005-08-10 |
DE69332829D1 (en) | 2003-05-08 |
DE69332829T2 (en) | 2004-01-22 |
FI950042A0 (en) | 1995-01-04 |
CA2139670C (en) | 2005-09-27 |
OA10123A (en) | 1996-12-18 |
ES2196009T3 (en) | 2003-12-16 |
NZ254108A (en) | 1995-12-21 |
JPH07508652A (en) | 1995-09-28 |
KR950702634A (en) | 1995-07-29 |
EP0673427A1 (en) | 1995-09-27 |
FI950042A (en) | 1995-01-24 |
US6027910A (en) | 2000-02-22 |
WO1994001567A1 (en) | 1994-01-20 |
EP0673427B1 (en) | 2003-04-02 |
AU4565393A (en) | 1994-01-31 |
JP2004254700A (en) | 2004-09-16 |
ATE236257T1 (en) | 2003-04-15 |
PT673427E (en) | 2003-08-29 |
RU95105899A (en) | 1997-03-20 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request |