CA2079454A1 - Steroid 5.alpha.-reductases - Google Patents

Steroid 5.alpha.-reductases

Info

Publication number
CA2079454A1
CA2079454A1 CA002079454A CA2079454A CA2079454A1 CA 2079454 A1 CA2079454 A1 CA 2079454A1 CA 002079454 A CA002079454 A CA 002079454A CA 2079454 A CA2079454 A CA 2079454A CA 2079454 A1 CA2079454 A1 CA 2079454A1
Authority
CA
Canada
Prior art keywords
preparation
reductases
alpha
steroid
enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002079454A
Other languages
French (fr)
Other versions
CA2079454C (en
Inventor
Stefan Andersson
David W. Russell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Texas System
Original Assignee
Stefan Andersson
David W. Russell
The Board Of Regents Of The University Of Texas System
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stefan Andersson, David W. Russell, The Board Of Regents Of The University Of Texas System filed Critical Stefan Andersson
Publication of CA2079454A1 publication Critical patent/CA2079454A1/en
Application granted granted Critical
Publication of CA2079454C publication Critical patent/CA2079454C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
    • C12Y103/01022—3-Oxo-5alpha-steroid 4-dehydrogenase (NADP+) (1.3.1.22), i.e. cortisone alpha-reductase

Abstract

Disclosed are methods and compositions for the preparation of human steroid 5.alpha.-reductases by recombinant means, as well as for the use of these enzymes in screening assays for the identification of compounds which have the ability to inhibit or otherwise alter the enzymatic function of these enzymes. In particular embodiments, methods and compositions are disclosed for the preparation of steroid 5.alpha.-reductases from human sources, as well as DNA sequences which encode these enzymes. These se-quences may be used directly in the preparation of genetic constructs, or may be employed in the preparation of hybridization probes for the selection of enzyme-encoding sequences from other sources.
CA002079454A 1990-04-30 1991-04-25 Steroid 5.alpha.-reductases Expired - Fee Related CA2079454C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US51766190A 1990-04-30 1990-04-30
US517,661 1990-04-30
PCT/US1991/002866 WO1991017251A1 (en) 1990-04-30 1991-04-25 STEROID 5α-REDUCTASES

Publications (2)

Publication Number Publication Date
CA2079454A1 true CA2079454A1 (en) 1991-10-31
CA2079454C CA2079454C (en) 2002-02-26

Family

ID=24060703

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002079454A Expired - Fee Related CA2079454C (en) 1990-04-30 1991-04-25 Steroid 5.alpha.-reductases

Country Status (8)

Country Link
US (2) US5422262A (en)
EP (1) EP0528906B1 (en)
JP (1) JP3294844B2 (en)
AT (1) ATE129013T1 (en)
AU (1) AU642501B2 (en)
CA (1) CA2079454C (en)
DE (1) DE69113795T2 (en)
WO (1) WO1991017251A1 (en)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU642501B2 (en) * 1990-04-30 1993-10-21 Board Of Regents, The University Of Texas System Steroid 5alpha-reductases
US5576191A (en) * 1994-06-17 1996-11-19 Immunex Corporation Cytokine that binds ST2
US5656613A (en) * 1995-01-04 1997-08-12 Merck & Co., Inc. Treatment of hyperandrogenic conditions
US5834248A (en) * 1995-02-10 1998-11-10 Millennium Pharmaceuticals Inc. Compositions and methods using rchd534, a gene uregulated by shear stress
US6359194B1 (en) * 1995-02-10 2002-03-19 Millennium Pharmaceuticals, Inc. Compositions and methods for the treatment and diagnosis of cardiovascular disease
US20020170077A1 (en) * 1995-02-10 2002-11-14 Millennium Pharmaceuticals, Inc. Compositions and methods for the treatment and diagnosis of cardiovascular disease
IT1276662B1 (en) * 1995-04-04 1997-11-03 Uni Degli Studi Camerino POLYNUCLEOTIDIC VACCINES
WO1997020930A1 (en) * 1995-12-07 1997-06-12 Baylor College Of Medicine Urogenital sinus derived growth factor nucleotide and amino acid sequences
EP0904277A4 (en) 1996-02-16 2003-01-22 Millennium Pharm Inc Compositions and methods for the treatment and diagnosis of cardiovascular disease
WO1997038728A1 (en) * 1996-04-15 1997-10-23 Dyad Pharmaceutical Corporation Combination therapy for androgenic alopecia with antisense oligonucleotides and minoxidil
US6143950A (en) * 1996-04-18 2000-11-07 The Salk Institute For Biological Studies Plant steroid 5α reductase, DET2
ATE301674T1 (en) * 1996-05-15 2005-08-15 Altana Pharma Ag METHOD FOR THE SEPARATION AND DETERMINATION OF HYDROPHOBIC PROTEINS BY THIN LAYER CHROMATOGRAPHY
WO1999037986A2 (en) 1998-01-23 1999-07-29 University Of Southern California Androgen-metabolic gene mutations and prostate cancer risk
CA2330611A1 (en) 1998-05-22 1999-12-02 The Board Of Trustees Of The Leland Stanford Junior University Bifunctional molecules and therapies based thereon
WO2001079504A1 (en) * 2000-04-13 2001-10-25 Millennium Pharmaceuticals, Inc. 23155 NOVEL PROTEIN HUMAN 5-α REDUCTASES AND USES THEREFOR
US20020177566A1 (en) * 2000-04-25 2002-11-28 David Pritchard Nucleic acid sequences associated with baldness
US6469150B1 (en) * 2000-05-22 2002-10-22 Pharmacopeia, Inc. Cloning and characterization of genes encoding bradykinin B1 receptor homologues from five mammalian species
EP1352054A2 (en) 2000-10-30 2003-10-15 Pharmacia Corporation Aspergillus ochraceus 11 alpha hydrolase and oxidoreductase
IL155691A0 (en) * 2000-11-09 2003-11-23 Astrazeneca Ab Oral pharmaceutical composition containing a block copolymer
JP2002236307A (en) * 2001-02-09 2002-08-23 Asahi Optical Co Ltd Zoom stroboscopic device
US7635598B2 (en) * 2004-07-08 2009-12-22 Biosearch Technologies, Inc. Inducible fluorescence assay
US20070036740A1 (en) * 2004-10-06 2007-02-15 Reed Kenneth C Modulation of hair growth
EP3827747A1 (en) 2005-04-28 2021-06-02 Otsuka Pharmaceutical Co., Ltd. Pharma-informatics system
EP1945240B1 (en) 2005-09-16 2016-12-28 Raptor Pharmaceutical Inc Compositions comprising receptor-associated protein (rap) variants specific for cr-containing proteins and uses thereof
FR2903999B1 (en) * 2006-07-19 2008-09-05 Galderma Res & Dev S N C Snc MODULATORS OF SC4MOL IN THE TREATMENT OF ACNE OR HYPERSEBORRHEA
EP2398500B1 (en) 2009-02-20 2019-03-13 2-BBB Medicines B.V. Glutathione-based drug delivery system
US20120156138A1 (en) * 2009-04-14 2012-06-21 Smith Larry J Methods and Compositions for the Treatment of Medical Conditions Involving Cellular Reprogramming
SG10201402069PA (en) 2009-05-06 2014-07-30 Lab Skin Care Inc Dermal delivery compositions comprising active agent-calcium phosphate particle complexes and methods of using the same

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU642501B2 (en) * 1990-04-30 1993-10-21 Board Of Regents, The University Of Texas System Steroid 5alpha-reductases

Also Published As

Publication number Publication date
AU642501B2 (en) 1993-10-21
DE69113795T2 (en) 1996-03-28
EP0528906A1 (en) 1993-03-03
JPH05507405A (en) 1993-10-28
US5422262A (en) 1995-06-06
CA2079454C (en) 2002-02-26
ATE129013T1 (en) 1995-10-15
JP3294844B2 (en) 2002-06-24
DE69113795D1 (en) 1995-11-16
US5679521A (en) 1997-10-21
AU7857991A (en) 1991-11-27
EP0528906B1 (en) 1995-10-11
WO1991017251A1 (en) 1991-11-14

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